Aplica??o de t?cnicas independentes de cultivo na detec??o de bact?rias de import?ncia agropecu?ria

• Autor(a) principal: Carvalho, Bruno Oliveira de
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
• Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/2320
• topic: Mastitis; Soils; Independent cultive techniques; Mastite; Solos; Paenibacillus larvae; DGGE; Independente de cultivo; Agronomia

The knowledge of bacterial genetic information has enable advances in many areas allowing detecting microorganisms and evaluating the production of virulence factors and resistance in certain populations. This knowledge allows the detection of microorganisms, as well as the evaluation of virulence and resistance factors production in certain populations. More recently, molecular diagnosis based on the detection of specific fragments from these agents has been implemented in no viable bacterial cultivation environments or hampered by biotic or abiotic conditions. Besides specific agent detection, bacterial diversity analysis might be an important criterion, such as assessing soil quality. Cultivation has been the most common way for evaluating bacterial diversity. However, most of soil bacteria are not cultivable; therefore, the employment of molecular tools has been increasingly used on this sort of analysis. This survey aimed standardizing the use of cultivation independent techniques for granting three agriculture sectors demands. The first chapter standardized the employment of DGGE technique for analyzing milk bacterial diversity from mastitis and healthy teats belonging to the same animal. Results pointed to differences on bacterial profile from both groups. The second chapter evaluated the impact of the employment of drill cuttings with castor beans and cramble pies association as agricultural fertilizers by DGGE analysis. The application of this technique was efficient for evaluating treatments, as well as, confirming the treatments contribution for increasing bacterial diversity in soil samples. The third chapter compared the cultivation technique sensitivity for American Foul Brood (AFB) diagnosis, a bee disease caused by Paenibacillus larvae, to cultivation independent technique by PCR. This survey demonstrated that sensitivity of the technique was greater than that one by P. larvae cultivation technique. Thus, the present survey concluded that cultivation independent techniques were useful for granting agriculture sectors demands.