A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos

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Autor(a) principal: Santos, Raoni da Conceição dos
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/9427
Resumo: A grelina é um hormônio orexigênico produzido principalmente pelo estômago. Além de seu efeito indutor da fome a grelina também afeta uma série de variáveis fisiológicas como o controle neuroendócrino, a função autonômica e cardiovascular, a resposta ao estresse e os comportamentos análogos à ansiedade e depressão. O núcleo paraventricular do hipotálamo (PVN) e a amígdala basolateral (BLA) são núcleos encefálicos importantes na integração de diversos dos efeitos da grelina. Para elucidar os efeitos da grelina sobre a atividade de neurônios do PVN nós utilizamos registros eletrofisiológicos extracelulares e intracelulares em fatias de encéfalo ex vivo, e observamos que a grelina pode aumentar ou diminuir a frequência de disparos dos neurônios do PVN, porém, os efeitos excitatórios são diretos, enquanto os efeitos inibitórios são indiretos. Verificamos também que a grelina afeta a maioria dos neurônios desse núcleo sejam eles pré-autonômicos, neuroendócrinos parvocelulares ou neuroendócrinos magnocelulares; e que a maioria dos neurônios TRH, CRH e OT do PVN é hiperpolarizada pela grelina. Similarmente, realizamos registros intracelulares de neurônios da BLA e verificamos que a grelina é capaz de aumentar ou diminuir o potencial de membrana de neurônios deste núcleo. Em seguida, analisamos as alterações comportamentais causadas pela privação alimentar, um estímulo fisiológico que aumenta a grelina plasmática, e da administração exógena de grelina sobre os comportamentos análogos à ansiedade e sobre a atividade exploratória. Nestes experimentos verificamos que a privação alimentar diminui o comportamento análogo à ansiedade no labirinto em cruz elevado (4,55 ± 0,97 vs 13,82 ± 3,02 %; p = 0,01; teste t não pareado) e não altera a atividade exploratória no campo aberto e no campo aberto modificado. No entanto, a grelina não afetou o comportamento análogo à ansiedade no labirinto em cruz elevado. Em conjunto os resultados descritos nessa tese demonstram que a grelina afeta núcleos encefálicos envolvidos com o controle de diversas funções fisiológicas, e provê um substrato neurobiológico para algumas funções deste hormônio. No entanto, não observamos efeitos comportamentais da grelina, o que sugere a necessidade de estudos futuros para elucidar a participação do hormônio nas respostas comportamentais que medeiam a resposta à situações de déficit calórico, como a privação alimentar.
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spelling Santos, Raoni da Conceição dosReis, Luis CarlosCPF: 484.252.577-00Côrtes, Wellington da SilvaMalvar, David do CarmoTrevenzoli, lsis HaraBorges, Danilo LustrinoCPF: 123.652.537-09http://lattes.cnpq.br/81672797709015382023-12-21T18:39:14Z2023-12-21T18:39:14Z2019-02-15SANTOS, Raoni da Conceição dos. A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos. 2019. 140 f. Tese (Doutorado em Ciências Fisiológicas) - Instituto de Ciências Biológicas e da Saúde, Departamento de Ciências Fisiológicas, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2019.https://rima.ufrrj.br/jspui/handle/20.500.14407/9427A grelina é um hormônio orexigênico produzido principalmente pelo estômago. Além de seu efeito indutor da fome a grelina também afeta uma série de variáveis fisiológicas como o controle neuroendócrino, a função autonômica e cardiovascular, a resposta ao estresse e os comportamentos análogos à ansiedade e depressão. O núcleo paraventricular do hipotálamo (PVN) e a amígdala basolateral (BLA) são núcleos encefálicos importantes na integração de diversos dos efeitos da grelina. Para elucidar os efeitos da grelina sobre a atividade de neurônios do PVN nós utilizamos registros eletrofisiológicos extracelulares e intracelulares em fatias de encéfalo ex vivo, e observamos que a grelina pode aumentar ou diminuir a frequência de disparos dos neurônios do PVN, porém, os efeitos excitatórios são diretos, enquanto os efeitos inibitórios são indiretos. Verificamos também que a grelina afeta a maioria dos neurônios desse núcleo sejam eles pré-autonômicos, neuroendócrinos parvocelulares ou neuroendócrinos magnocelulares; e que a maioria dos neurônios TRH, CRH e OT do PVN é hiperpolarizada pela grelina. Similarmente, realizamos registros intracelulares de neurônios da BLA e verificamos que a grelina é capaz de aumentar ou diminuir o potencial de membrana de neurônios deste núcleo. Em seguida, analisamos as alterações comportamentais causadas pela privação alimentar, um estímulo fisiológico que aumenta a grelina plasmática, e da administração exógena de grelina sobre os comportamentos análogos à ansiedade e sobre a atividade exploratória. Nestes experimentos verificamos que a privação alimentar diminui o comportamento análogo à ansiedade no labirinto em cruz elevado (4,55 ± 0,97 vs 13,82 ± 3,02 %; p = 0,01; teste t não pareado) e não altera a atividade exploratória no campo aberto e no campo aberto modificado. No entanto, a grelina não afetou o comportamento análogo à ansiedade no labirinto em cruz elevado. Em conjunto os resultados descritos nessa tese demonstram que a grelina afeta núcleos encefálicos envolvidos com o controle de diversas funções fisiológicas, e provê um substrato neurobiológico para algumas funções deste hormônio. No entanto, não observamos efeitos comportamentais da grelina, o que sugere a necessidade de estudos futuros para elucidar a participação do hormônio nas respostas comportamentais que medeiam a resposta à situações de déficit calórico, como a privação alimentar.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorGhrelin is an orexigenic hormone produced by the stomach. Additional to its effects on food intake, ghrelin also affects a series of physiological variables, such as the neuroendocrine control, autonomic and cardiovascular function, the response to stress, and anxiety-like and depression-like behaviors. The paraventricular hypothalamic nucleus (PVN) and the basolateral amygdala (BLA) are brain nuclei involved in the integration of several of ghrelin’s effects. To elucidate the effects of ghrelin on the activity of PVN neurons we used extracellular and intracellular electrophysiological recordings in ex vivo brain slices and observed that ghrelin may increase or decrease the spike frequency of PVN neurons; however, the excitatory effects are direct while the inhibitory effects are indirect. We also observed that ghrelin affects the majority of neurons in this nucleus, whether they are pre-autonomic, neuroendocrine parvocellular or neuroendocrine magnocellular; and that the most TRH, CRH and OT PVN neurons are hyperpolarized in response to ghrelin. Similarly, we performed intracellular recordings in BLA neurons and showed that ghrelin increases or decreases the membrane potential in neurons in this nucleus. Next, we analyzed the behavioral alterations caused by food deprivation, a physiological stimulus that increases plasma ghrelin, and the exogenous administration of ghrelin on anxiety-like behaviors and exploratory activity. In these experiments we showed that food deprivation decreases anxiety-like behavior in the elevated plus maze (4.55 ± 0.97 vs 13.82 ± 3.02 %; p = 0.01; unpaired t test) and does not change exploratory activity on the open field and modified open field. However, ghrelin did not affect anxiety-like behavior in the elevated plus maze. Taken together the results described in this thesis showed that ghrelin affects brain nuclei involved in the control of several physiological functions, thus providing a neurobiological substrate for ghrelin’s effects for some functions of this hormone. However, we did not observe behavioral alterations caused by ghrelin, which suggests that future studies are necessary to elucidate the participation of ghrelin on the behavioral responses that mediate the response to situations of caloric deficits, such as food deprivation.application/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em Ciências FisiológicasUFRRJBrasilInstituto de Ciências Biológicas e da Saúdegrelinacontrole da ingestão alimentarcomportamento análogo à ansiedadeamígdala basolateralnúcleo paraventricular do hipotálamoghrelinfood intake controlanxiety-like behaviorbasolateral amygdalaparaventricular hypothalamic nucleusFisiologiaFarmacologiaA influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratosThe influence of ghrelin on anxietylike behavior and the activity of paraventricular hypothalamic nucleus and basolateral amygdala neurons in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisABTAHI, S. et al. 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dc.title.por.fl_str_mv A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos
dc.title.alternative.eng.fl_str_mv The influence of ghrelin on anxietylike behavior and the activity of paraventricular hypothalamic nucleus and basolateral amygdala neurons in rats
title A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos
spellingShingle A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos
Santos, Raoni da Conceição dos
grelina
controle da ingestão alimentar
comportamento análogo à ansiedade
amígdala basolateral
núcleo paraventricular do hipotálamo
ghrelin
food intake control
anxiety-like behavior
basolateral amygdala
paraventricular hypothalamic nucleus
Fisiologia
Farmacologia
title_short A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos
title_full A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos
title_fullStr A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos
title_full_unstemmed A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos
title_sort A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos
author Santos, Raoni da Conceição dos
author_facet Santos, Raoni da Conceição dos
author_role author
dc.contributor.author.fl_str_mv Santos, Raoni da Conceição dos
dc.contributor.advisor1.fl_str_mv Reis, Luis Carlos
dc.contributor.advisor1ID.fl_str_mv CPF: 484.252.577-00
dc.contributor.referee1.fl_str_mv Côrtes, Wellington da Silva
dc.contributor.referee2.fl_str_mv Malvar, David do Carmo
dc.contributor.referee3.fl_str_mv Trevenzoli, lsis Hara
dc.contributor.referee4.fl_str_mv Borges, Danilo Lustrino
dc.contributor.authorID.fl_str_mv CPF: 123.652.537-09
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8167279770901538
contributor_str_mv Reis, Luis Carlos
Côrtes, Wellington da Silva
Malvar, David do Carmo
Trevenzoli, lsis Hara
Borges, Danilo Lustrino
dc.subject.por.fl_str_mv grelina
controle da ingestão alimentar
comportamento análogo à ansiedade
amígdala basolateral
núcleo paraventricular do hipotálamo
topic grelina
controle da ingestão alimentar
comportamento análogo à ansiedade
amígdala basolateral
núcleo paraventricular do hipotálamo
ghrelin
food intake control
anxiety-like behavior
basolateral amygdala
paraventricular hypothalamic nucleus
Fisiologia
Farmacologia
dc.subject.eng.fl_str_mv ghrelin
food intake control
anxiety-like behavior
basolateral amygdala
paraventricular hypothalamic nucleus
dc.subject.cnpq.fl_str_mv Fisiologia
Farmacologia
description A grelina é um hormônio orexigênico produzido principalmente pelo estômago. Além de seu efeito indutor da fome a grelina também afeta uma série de variáveis fisiológicas como o controle neuroendócrino, a função autonômica e cardiovascular, a resposta ao estresse e os comportamentos análogos à ansiedade e depressão. O núcleo paraventricular do hipotálamo (PVN) e a amígdala basolateral (BLA) são núcleos encefálicos importantes na integração de diversos dos efeitos da grelina. Para elucidar os efeitos da grelina sobre a atividade de neurônios do PVN nós utilizamos registros eletrofisiológicos extracelulares e intracelulares em fatias de encéfalo ex vivo, e observamos que a grelina pode aumentar ou diminuir a frequência de disparos dos neurônios do PVN, porém, os efeitos excitatórios são diretos, enquanto os efeitos inibitórios são indiretos. Verificamos também que a grelina afeta a maioria dos neurônios desse núcleo sejam eles pré-autonômicos, neuroendócrinos parvocelulares ou neuroendócrinos magnocelulares; e que a maioria dos neurônios TRH, CRH e OT do PVN é hiperpolarizada pela grelina. Similarmente, realizamos registros intracelulares de neurônios da BLA e verificamos que a grelina é capaz de aumentar ou diminuir o potencial de membrana de neurônios deste núcleo. Em seguida, analisamos as alterações comportamentais causadas pela privação alimentar, um estímulo fisiológico que aumenta a grelina plasmática, e da administração exógena de grelina sobre os comportamentos análogos à ansiedade e sobre a atividade exploratória. Nestes experimentos verificamos que a privação alimentar diminui o comportamento análogo à ansiedade no labirinto em cruz elevado (4,55 ± 0,97 vs 13,82 ± 3,02 %; p = 0,01; teste t não pareado) e não altera a atividade exploratória no campo aberto e no campo aberto modificado. No entanto, a grelina não afetou o comportamento análogo à ansiedade no labirinto em cruz elevado. Em conjunto os resultados descritos nessa tese demonstram que a grelina afeta núcleos encefálicos envolvidos com o controle de diversas funções fisiológicas, e provê um substrato neurobiológico para algumas funções deste hormônio. No entanto, não observamos efeitos comportamentais da grelina, o que sugere a necessidade de estudos futuros para elucidar a participação do hormônio nas respostas comportamentais que medeiam a resposta à situações de déficit calórico, como a privação alimentar.
publishDate 2019
dc.date.issued.fl_str_mv 2019-02-15
dc.date.accessioned.fl_str_mv 2023-12-21T18:39:14Z
dc.date.available.fl_str_mv 2023-12-21T18:39:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Raoni da Conceição dos. A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos. 2019. 140 f. Tese (Doutorado em Ciências Fisiológicas) - Instituto de Ciências Biológicas e da Saúde, Departamento de Ciências Fisiológicas, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2019.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/9427
identifier_str_mv SANTOS, Raoni da Conceição dos. A influência da grelina sobre o comportamento análogo à ansiedade e a atividade de neurônios do núcleo paraventricular do hipotálamo e da amígdala basolateral em ratos. 2019. 140 f. Tese (Doutorado em Ciências Fisiológicas) - Instituto de Ciências Biológicas e da Saúde, Departamento de Ciências Fisiológicas, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2019.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/9427
dc.language.iso.fl_str_mv por
language por
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