Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos

Detalhes bibliográficos
Autor(a) principal: Moreira, Rodrigo Mencalha
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/tede/896
Resumo: Brain serotonin (5-HT) depletion during the neonatal period lead a plastic cytoarchitecture change of the serotonergic system influencing directly several brain systems ontogeny development. This dissertation was aimed to elucidate the influence of brain 5-HT depletion during the neonatal period on the metabolic programming and the behavioral expression of adulthood rats. Brain 5-HT depletion was raised with the subcutaneous (s.c) administration of 100 mg. Kg-1 para-chlorophenylalanine (pCPA), a competitive inhibitor of the tryptophan hydroxylase enzyme, from the eighth at sixteenth post-natal days. In order to investigate the influence of pCPA on the subject metabolic programming, during and after treatment, the milk intake and body weight gain from the neonatal period to adulthood were evaluated. Furthermore, evaluating indirectly this influence on the energetic metabolism, the repercussions on the glycemic homeostasis through of the glycemia baseline measurements and under fasting in the adult rat were investigated. Around the ingestive behavior, baseline conditions paradigm and under fasting were made. In these experimental trials, water intake, ration, 2% sucrose and 0.3M NaCl were evaluated. Also, the 0.3 M NaCl intake after sodium depletion (furosemide 20mg. Kg-1, s.c) and water intake after hypertonic stimulus (1.0 M NaCl, s.c) were measured. The behavioral expression was evaluated through models involved with emotional-behavioral signals. In this analysis, the rat s anxiety status was investigated through of open field test, elevated plus-maze (EPM), social interaction and forced swimming. The results showed that treatment with pCPA reduces milk intake (P?0.001) and therefore the body weight gain in the neonatal period (P?0.001), claiming to be smaller until adulthood (P?0.01). The glycemic homeostasis was also affected reflected in lower pCPA-male (P?0.01) and pCPA-female (P?0.001) levels after 24 hours of fasting. About to baseline intake, a higher pCPA-female sodium appetite (P?0.05), related to a greater demand for 2% sucrose solution in both females (P?0,001) were observed. Under fasting, a lower male-pCPA cumulative ration intake at 60 min (P?0.05) and a higher female pCPA at 60 (P?0.05) and 120 min (P?0.01) were observed. Furthermore, after water deprivation, a higher pCPA-female cumulative water intake at 120 min (P?0.05) compared with pCPA-male was rated. Was also observed a greater pCPA-male water intake at 120 and 180 min (P?0.001) after hypertonic stimulus, however, it was not observed significant difference in the natriorexigenic responses in sodium depleted rats. In addition, pCPA male showed a evident anxiolytic activity in EPM test expressed by the higher time spent in the open arm (O.A) (P?0.05), higher head-dipping in the O.A episodes (P?0.001), less S.A.P. (P?0.001) and grooming episodes (P?0.01). Therefore, these results showed that 5-HT depletion during the neonatal period programme the energetic metabolic homeostasis and the behavioral expression of adulthood rats.
id UFRRJ-1_e53c5c1bf8adcd6833032e1858362ee5
oai_identifier_str oai:localhost:tede/896
network_acronym_str UFRRJ-1
network_name_str Biblioteca Digital de Teses e Dissertações da UFRRJ
repository_id_str
spelling Reis, Luis Carlos484.252.577-00http://lattes.cnpq.br/2679836949147357Cortes, Wellington da Silva856.794.707-30http://lattes.cnpq.br/1305510562756172084.380.657-59http://lattes.cnpq.br/5427334834841231Moreira, Rodrigo Mencalha2016-04-28T20:18:30Z2009-03-102008-08-28MOREIRA, Rodrigo Mencalha. Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos. 2008. 69 f. Disserta??o (Mestrado em Medicina Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008.https://tede.ufrrj.br/jspui/handle/tede/896Brain serotonin (5-HT) depletion during the neonatal period lead a plastic cytoarchitecture change of the serotonergic system influencing directly several brain systems ontogeny development. This dissertation was aimed to elucidate the influence of brain 5-HT depletion during the neonatal period on the metabolic programming and the behavioral expression of adulthood rats. Brain 5-HT depletion was raised with the subcutaneous (s.c) administration of 100 mg. Kg-1 para-chlorophenylalanine (pCPA), a competitive inhibitor of the tryptophan hydroxylase enzyme, from the eighth at sixteenth post-natal days. In order to investigate the influence of pCPA on the subject metabolic programming, during and after treatment, the milk intake and body weight gain from the neonatal period to adulthood were evaluated. Furthermore, evaluating indirectly this influence on the energetic metabolism, the repercussions on the glycemic homeostasis through of the glycemia baseline measurements and under fasting in the adult rat were investigated. Around the ingestive behavior, baseline conditions paradigm and under fasting were made. In these experimental trials, water intake, ration, 2% sucrose and 0.3M NaCl were evaluated. Also, the 0.3 M NaCl intake after sodium depletion (furosemide 20mg. Kg-1, s.c) and water intake after hypertonic stimulus (1.0 M NaCl, s.c) were measured. The behavioral expression was evaluated through models involved with emotional-behavioral signals. In this analysis, the rat s anxiety status was investigated through of open field test, elevated plus-maze (EPM), social interaction and forced swimming. The results showed that treatment with pCPA reduces milk intake (P?0.001) and therefore the body weight gain in the neonatal period (P?0.001), claiming to be smaller until adulthood (P?0.01). The glycemic homeostasis was also affected reflected in lower pCPA-male (P?0.01) and pCPA-female (P?0.001) levels after 24 hours of fasting. About to baseline intake, a higher pCPA-female sodium appetite (P?0.05), related to a greater demand for 2% sucrose solution in both females (P?0,001) were observed. Under fasting, a lower male-pCPA cumulative ration intake at 60 min (P?0.05) and a higher female pCPA at 60 (P?0.05) and 120 min (P?0.01) were observed. Furthermore, after water deprivation, a higher pCPA-female cumulative water intake at 120 min (P?0.05) compared with pCPA-male was rated. Was also observed a greater pCPA-male water intake at 120 and 180 min (P?0.001) after hypertonic stimulus, however, it was not observed significant difference in the natriorexigenic responses in sodium depleted rats. In addition, pCPA male showed a evident anxiolytic activity in EPM test expressed by the higher time spent in the open arm (O.A) (P?0.05), higher head-dipping in the O.A episodes (P?0.001), less S.A.P. (P?0.001) and grooming episodes (P?0.01). Therefore, these results showed that 5-HT depletion during the neonatal period programme the energetic metabolic homeostasis and the behavioral expression of adulthood rats.A deple??o cerebral de serotonina (5-HT) durante o per?odo neonatal acarreta uma altera??o pl?stica na citoarquitetura do sistema seroton?rgico influenciando diretamente o desenvolvimento ontog?nico de diversos sistemas cerebrais. Nesta disserta??o objetivou-se elucidar a influ?ncia da deple??o cerebral de 5-HT durante o per?odo neonatal sobre a programa??o metab?lica energ?tica e express?o comportamental de ratos adultos. A deple??o cerebral de 5-HT foi evocada com a administra??o subcut?nea (s.c) de 100 mg. Kg-1, de para-clorofenilalanina (pCPA), um inibidor competitivo da enzima triptofano hidroxilase, do oitavo ao d?cimo sexto dias p?s-natais. A fim de investigar a influ?ncia da pCPA, durante e ap?s o tratamento, sobre a programa??o metab?lica do indiv?duo, foram avaliadas a ingest?o de leite e o ganho de peso corporal do per?odo neonatal ? vida adulta. Ademais, avaliando indiretamente esta influ?ncia sobre o metabolismo energ?tico, foram investigadas as repercuss?es sobre a homeostase glic?mica atrav?s da mensura??o basal e sob jejum da glicemia do rato adulto. Em rela??o ao comportamento ingestivo foram realizados protocolos em condi??es basais e sob jejum. Nestes ensaios experimentais foram avaliadas as ingest?es de ?gua, ra??o, sacarose 2% e NaCl 0,3 M. Outrossim, foram mensuradas as ingest?es de NaCl 0,3 M ap?s deple??o de s?dio (furosemida 20mg. Kg-1, s.c) e de ?gua ap?s est?mulo hipert?nico (NaCl 1,0 M, s.c). A express?o comportamental foi avaliada atrav?s de modelos envolvidos com aspectos afetivo-comportamentais. Nesta an?lise, foi investigado o status de ansiedade dos ratos atrav?s dos testes de campo aberto, labirinto em cruz elevado (LCE), intera??o social e nata??o for?ada. Os resultados demonstraram que o tratamento com pCPA diminui a ingest?o de leite (P?0,001) e conseq?entemente o ganho de peso corporal no per?odo neonatal (P?0,001), sustentando-se menor at? a vida adulta (P?0,01). A homeostase glic?mica tamb?m foi afetada refletida nos menores n?veis glic?micos em machos-pCPA (P?0,01) e f?meas pCPA (P?0,001) ap?s jejum alimentar de 24 horas. Em rela??o ?s ingest?es basais, foi observado um maior apetite por s?dio em f?meas-pCPA (P?0,05), al?m de uma maior procura pela solu??o de sacarose 2% em ambas as f?meas (P?0,001). Sob jejum alimentar, foi observada uma menor ingest?o cumulativa de ra??o em machos-pCPA aos 120 min (P?0,05) e maior ingest?o em f?meas-pCPA aos 60 (P?0,05) e 120 min (P?0,01). Ademais, ap?s jejum h?drico, foi notada uma maior ingest?o cumulativa de ?gua em f?meas-pCPA aos 120 min (P?0,05) em rela??o aos machos-pCPA. Observou-se ainda uma maior ingest?o de ?gua nos machos-pCPA aos 120 e 180 min (P?0,001) ap?s est?mulo hipert?nico, por?m, n?o foi observada diferen?a significativa na resposta natriorexig?nica em ratos s?dio-depletados. Al?m disso, foi observada uma evidente atividade ansiol?tica no LCE expressa pelo maior tempo gasto no bra?o aberto (B.A) (P?0,05), maior n?mero de epis?dios de head-dipping no B.A (P?0,001), menos epis?dios de S.AP. (P?0,001) e grooming (P?0,01). Em resumo, estes resultados demonstram que a deple??o de 5-HT durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos.Made available in DSpace on 2016-04-28T20:18:30Z (GMT). No. of bitstreams: 1 2008 - Rodrigo Mencalha Moreira.pdf: 2348355 bytes, checksum: 249dc9822a474bb30086d8952df6f1f2 (MD5) Previous issue date: 2008-08-28application/pdfhttps://tede.ufrrj.br/retrieve/60299/2008%20-%20Rodrigo%20Mencalha%20Moreira.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas)UFRRJBrasilInstituto de Veterin?riaserotoninadesenvolvimento ontog?nicoprograma??o metab?licaserotoninontogeny developmentmetabolic programmingMedicina Veterin?riaDeple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultosBrain serotonin depletion during neonatal period programme energetic metabolic homeostasis and behavioral expression of adulthood ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2008 - Rodrigo Mencalha Moreira.pdf.jpg2008 - Rodrigo Mencalha Moreira.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/tede/896/3/2008+-+Rodrigo+Mencalha+Moreira.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD53TEXT2008 - Rodrigo Mencalha Moreira.pdf.txt2008 - Rodrigo Mencalha Moreira.pdf.txttext/plain194413http://localhost:8080/tede/bitstream/tede/896/2/2008+-+Rodrigo+Mencalha+Moreira.pdf.txt180e8a597358238f86c7a8c1848c37f5MD52ORIGINAL2008 - Rodrigo Mencalha Moreira.pdf2008 - Rodrigo Mencalha Moreira.pdfapplication/pdf2348101http://localhost:8080/tede/bitstream/tede/896/1/2008+-+Rodrigo+Mencalha+Moreira.pdfe057e587c14df022d512a03e9b1e53b1MD51tede/8962022-06-15 13:14:03.28oai:localhost:tede/896Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-15T16:14:03Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false
dc.title.por.fl_str_mv Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos
dc.title.alternative.eng.fl_str_mv Brain serotonin depletion during neonatal period programme energetic metabolic homeostasis and behavioral expression of adulthood rats
title Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos
spellingShingle Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos
Moreira, Rodrigo Mencalha
serotonina
desenvolvimento ontog?nico
programa??o metab?lica
serotonin
ontogeny development
metabolic programming
Medicina Veterin?ria
title_short Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos
title_full Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos
title_fullStr Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos
title_full_unstemmed Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos
title_sort Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos
author Moreira, Rodrigo Mencalha
author_facet Moreira, Rodrigo Mencalha
author_role author
dc.contributor.advisor1.fl_str_mv Reis, Luis Carlos
dc.contributor.advisor1ID.fl_str_mv 484.252.577-00
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2679836949147357
dc.contributor.advisor-co1.fl_str_mv Cortes, Wellington da Silva
dc.contributor.advisor-co1ID.fl_str_mv 856.794.707-30
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/1305510562756172
dc.contributor.authorID.fl_str_mv 084.380.657-59
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5427334834841231
dc.contributor.author.fl_str_mv Moreira, Rodrigo Mencalha
contributor_str_mv Reis, Luis Carlos
Cortes, Wellington da Silva
dc.subject.por.fl_str_mv serotonina
desenvolvimento ontog?nico
programa??o metab?lica
topic serotonina
desenvolvimento ontog?nico
programa??o metab?lica
serotonin
ontogeny development
metabolic programming
Medicina Veterin?ria
dc.subject.eng.fl_str_mv serotonin
ontogeny development
metabolic programming
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description Brain serotonin (5-HT) depletion during the neonatal period lead a plastic cytoarchitecture change of the serotonergic system influencing directly several brain systems ontogeny development. This dissertation was aimed to elucidate the influence of brain 5-HT depletion during the neonatal period on the metabolic programming and the behavioral expression of adulthood rats. Brain 5-HT depletion was raised with the subcutaneous (s.c) administration of 100 mg. Kg-1 para-chlorophenylalanine (pCPA), a competitive inhibitor of the tryptophan hydroxylase enzyme, from the eighth at sixteenth post-natal days. In order to investigate the influence of pCPA on the subject metabolic programming, during and after treatment, the milk intake and body weight gain from the neonatal period to adulthood were evaluated. Furthermore, evaluating indirectly this influence on the energetic metabolism, the repercussions on the glycemic homeostasis through of the glycemia baseline measurements and under fasting in the adult rat were investigated. Around the ingestive behavior, baseline conditions paradigm and under fasting were made. In these experimental trials, water intake, ration, 2% sucrose and 0.3M NaCl were evaluated. Also, the 0.3 M NaCl intake after sodium depletion (furosemide 20mg. Kg-1, s.c) and water intake after hypertonic stimulus (1.0 M NaCl, s.c) were measured. The behavioral expression was evaluated through models involved with emotional-behavioral signals. In this analysis, the rat s anxiety status was investigated through of open field test, elevated plus-maze (EPM), social interaction and forced swimming. The results showed that treatment with pCPA reduces milk intake (P?0.001) and therefore the body weight gain in the neonatal period (P?0.001), claiming to be smaller until adulthood (P?0.01). The glycemic homeostasis was also affected reflected in lower pCPA-male (P?0.01) and pCPA-female (P?0.001) levels after 24 hours of fasting. About to baseline intake, a higher pCPA-female sodium appetite (P?0.05), related to a greater demand for 2% sucrose solution in both females (P?0,001) were observed. Under fasting, a lower male-pCPA cumulative ration intake at 60 min (P?0.05) and a higher female pCPA at 60 (P?0.05) and 120 min (P?0.01) were observed. Furthermore, after water deprivation, a higher pCPA-female cumulative water intake at 120 min (P?0.05) compared with pCPA-male was rated. Was also observed a greater pCPA-male water intake at 120 and 180 min (P?0.001) after hypertonic stimulus, however, it was not observed significant difference in the natriorexigenic responses in sodium depleted rats. In addition, pCPA male showed a evident anxiolytic activity in EPM test expressed by the higher time spent in the open arm (O.A) (P?0.05), higher head-dipping in the O.A episodes (P?0.001), less S.A.P. (P?0.001) and grooming episodes (P?0.01). Therefore, these results showed that 5-HT depletion during the neonatal period programme the energetic metabolic homeostasis and the behavioral expression of adulthood rats.
publishDate 2008
dc.date.issued.fl_str_mv 2008-08-28
dc.date.available.fl_str_mv 2009-03-10
dc.date.accessioned.fl_str_mv 2016-04-28T20:18:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOREIRA, Rodrigo Mencalha. Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos. 2008. 69 f. Disserta??o (Mestrado em Medicina Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/tede/896
identifier_str_mv MOREIRA, Rodrigo Mencalha. Deple??o cerebral de serotonina durante o per?odo neonatal programa a homeostase metab?lica energ?tica e a express?o comportamental de ratos adultos. 2008. 69 f. Disserta??o (Mestrado em Medicina Veterin?ria) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2008.
url https://tede.ufrrj.br/jspui/handle/tede/896
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.publisher.program.fl_str_mv Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas)
dc.publisher.initials.fl_str_mv UFRRJ
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Veterin?ria
publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ
instname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
instname_str Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron_str UFRRJ
institution UFRRJ
reponame_str Biblioteca Digital de Teses e Dissertações da UFRRJ
collection Biblioteca Digital de Teses e Dissertações da UFRRJ
bitstream.url.fl_str_mv http://localhost:8080/tede/bitstream/tede/896/3/2008+-+Rodrigo+Mencalha+Moreira.pdf.jpg
http://localhost:8080/tede/bitstream/tede/896/2/2008+-+Rodrigo+Mencalha+Moreira.pdf.txt
http://localhost:8080/tede/bitstream/tede/896/1/2008+-+Rodrigo+Mencalha+Moreira.pdf
bitstream.checksum.fl_str_mv cc73c4c239a4c332d642ba1e7c7a9fb2
180e8a597358238f86c7a8c1848c37f5
e057e587c14df022d512a03e9b1e53b1
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)
repository.mail.fl_str_mv bibliot@ufrrj.br||bibliot@ufrrj.br
_version_ 1800313456644063232