Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares

Detalhes bibliográficos
Autor(a) principal: Barbosa, Rodrigo Rocha
Data de Publicação: 2015
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/9162
Resumo: A quimiotaxonomia baseada em perfis de hidrocarbonetos cuticulares (HCs) é uma ferramenta taxonômica que tem sido utilizada em insetos desde a década de 70 e sua utilização é baseada em sua espécie-especificidade. Em Diptera, estudos importantes têm sido publicados demonstrando que HCs podem ser utilizados em quimiotaxonomia, sendo úteis inclusive para distinção de espécies crípticas. A família Sarcophagidae possui espécies de importância forense, que são pioneiras na degradação cadavérica e aquelas dos gêneros Peckia Robineau-Desvoidy, 1830 e Oxysarcodexia Townsend, 1917 são encontradas frequentemente em carcaças de animais e cadáveres humanos. Este estudo teve como objetivo descrever, através da cromatografia gasosa acoplada à espectrometria de massas (CG-EM), os perfis dos HCs dos adultos de nove espécies de Sarcophagidae, considerando o sexo e a distribuição geográfica, além de comparar os perfis resultantes com os de outras espécies das famílias Calliphoridae, Muscidae e Stratiomyidae. As extrações dos HCs foram feitas em triplicata usando dez fêmeas e/ou dez machos para cada espécie coletadas em campo. A metodologia de extração e análise seguiu os protocolos anteriormente descritos. Utilizamos o CG-EM do Setor de Agrotóxico do Laboratório de Toxicologia da Fundação Oswaldo Cruz. Foram identificados apenas os HCs com comprimento de cadeia entre 21 e 40 carbonos, por serem os mais comumente encontrados nos insetos. A abundância relativa de cada pico foi obtida calculando sua área em relação à área total da amostra, sendo identificados apenas aqueles representando mais de 0,5%. Ao todo foram identificados 213 compostos classificados como n-alcanos, alcanos metil-ramificados e alcenos entre 22 e 39 carbonos na cadeia principal (CCP). Nos Sarcophagidae foram identificados 164 compostos variando entre 22 e 39 CCP e cada espécie apresentou um número diferente de HCs identificados. Oxysarcodexia thornax foi a espécie com maior quantidade de HCs identificados (48 compostos), contrastando com Peckia (Squamatodes) ingens que teve a menor (26 compostos). Todas as espécies de Sarcophagidae mostraram HCs específicos, como Malacophagomyia filamenta que foi a única que apresentou trimetilalcanos em sua composição, além de ser a com maior quantidade de HCs específicos (14), enquanto que Oxysarcodexia intona apresentou a menor quantidade deles (2). Algumas espécies apresentaram dimorfismo sexual, exemplificado por Ravinia belforti, cujos machos obtiveram 41 HCs com mais de 0,5% de abundância e as fêmeas 32. As populações de O. thornax de diferentes regiões do Brasil apresentaram cromatogramas semelhantes, mas, como esperado, houve oscilações nas abundâncias do HCs compartilhados e alguns poucos específicos por população. Os dois dendrogramas gerados para avaliar a similaridade entre os perfis de HCs das espécies resultaram em diferentes relações. Com a inclusão dos n-alcanos, o dendrograma mostrou boa correlação entre as espécies de Sarcophagidae, mas não entre os Calliphoridae e os Muscidae. No entanto, ao excluir os n-alcanos da análise, as espécies de Sarcophagidae continuaram com boa correlação, as de Calliphoridae e Muscidae ficaram melhor correlacionadas e Stratiomyidae, como esperado, se apresentou como a espécie mais dissimilar. Os resultados se mostraram promissores na utilização desta técnica na identificação de espécies de Sarcophagidae e de outras famílias de importância forense, bem como tornou possível a montagem de um banco de dados com informações acerca da quimiotaxonomia dessas espécies.
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spelling Barbosa, Rodrigo RochaQueiroz, Margareth Maria de Carvalho154.256.762-72http://lattes.cnpq.br/9360088536183208Braga, Marina ViannaNunes, Antônio José MayhéCosta, Janyra OliveiraThyssen, Patrícia JaquelineMello, Rubens Pinto de092.067.357-05http://lattes.cnpq.br/22360692102942862023-12-21T18:35:52Z2023-12-21T18:35:52Z2015-03-25BARBOSA, Rodrigo Rocha. Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares. 2015. 97 f. Tese (Doutorado em Biologia Animal) - Recursos Florestais e Engenharia Florestal Universidade Federal Rural do Rio de Janeiro, Seropédica, 2015.https://rima.ufrrj.br/jspui/handle/20.500.14407/9162A quimiotaxonomia baseada em perfis de hidrocarbonetos cuticulares (HCs) é uma ferramenta taxonômica que tem sido utilizada em insetos desde a década de 70 e sua utilização é baseada em sua espécie-especificidade. Em Diptera, estudos importantes têm sido publicados demonstrando que HCs podem ser utilizados em quimiotaxonomia, sendo úteis inclusive para distinção de espécies crípticas. A família Sarcophagidae possui espécies de importância forense, que são pioneiras na degradação cadavérica e aquelas dos gêneros Peckia Robineau-Desvoidy, 1830 e Oxysarcodexia Townsend, 1917 são encontradas frequentemente em carcaças de animais e cadáveres humanos. Este estudo teve como objetivo descrever, através da cromatografia gasosa acoplada à espectrometria de massas (CG-EM), os perfis dos HCs dos adultos de nove espécies de Sarcophagidae, considerando o sexo e a distribuição geográfica, além de comparar os perfis resultantes com os de outras espécies das famílias Calliphoridae, Muscidae e Stratiomyidae. As extrações dos HCs foram feitas em triplicata usando dez fêmeas e/ou dez machos para cada espécie coletadas em campo. A metodologia de extração e análise seguiu os protocolos anteriormente descritos. Utilizamos o CG-EM do Setor de Agrotóxico do Laboratório de Toxicologia da Fundação Oswaldo Cruz. Foram identificados apenas os HCs com comprimento de cadeia entre 21 e 40 carbonos, por serem os mais comumente encontrados nos insetos. A abundância relativa de cada pico foi obtida calculando sua área em relação à área total da amostra, sendo identificados apenas aqueles representando mais de 0,5%. Ao todo foram identificados 213 compostos classificados como n-alcanos, alcanos metil-ramificados e alcenos entre 22 e 39 carbonos na cadeia principal (CCP). Nos Sarcophagidae foram identificados 164 compostos variando entre 22 e 39 CCP e cada espécie apresentou um número diferente de HCs identificados. Oxysarcodexia thornax foi a espécie com maior quantidade de HCs identificados (48 compostos), contrastando com Peckia (Squamatodes) ingens que teve a menor (26 compostos). Todas as espécies de Sarcophagidae mostraram HCs específicos, como Malacophagomyia filamenta que foi a única que apresentou trimetilalcanos em sua composição, além de ser a com maior quantidade de HCs específicos (14), enquanto que Oxysarcodexia intona apresentou a menor quantidade deles (2). Algumas espécies apresentaram dimorfismo sexual, exemplificado por Ravinia belforti, cujos machos obtiveram 41 HCs com mais de 0,5% de abundância e as fêmeas 32. As populações de O. thornax de diferentes regiões do Brasil apresentaram cromatogramas semelhantes, mas, como esperado, houve oscilações nas abundâncias do HCs compartilhados e alguns poucos específicos por população. Os dois dendrogramas gerados para avaliar a similaridade entre os perfis de HCs das espécies resultaram em diferentes relações. Com a inclusão dos n-alcanos, o dendrograma mostrou boa correlação entre as espécies de Sarcophagidae, mas não entre os Calliphoridae e os Muscidae. No entanto, ao excluir os n-alcanos da análise, as espécies de Sarcophagidae continuaram com boa correlação, as de Calliphoridae e Muscidae ficaram melhor correlacionadas e Stratiomyidae, como esperado, se apresentou como a espécie mais dissimilar. Os resultados se mostraram promissores na utilização desta técnica na identificação de espécies de Sarcophagidae e de outras famílias de importância forense, bem como tornou possível a montagem de um banco de dados com informações acerca da quimiotaxonomia dessas espécies.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES, Brasil.The chemotaxonomy based on cuticular hydrocarbon profiles (CHs) is a taxonomic tool that has been used for insects since the 1970's and their use is based on the species-specificity. In Diptera, significant studies have been published showing that CHs can be used in chemotaxonomy, being useful even for distinction of cryptic species. The Sarcophagidae family include species of forensic importance, which are pioneers in cadaveric degradation and those of genres Peckia Robineau-Desvoidy, 1830 and Oxysarcodexia Townsend, 1917 are often found in animal carcasses and human corpses. This study aimed to describe, by gas chromatography coupled tomass spectrometry (GC-MS), the profiles of the CHs of adults from nine species of the family Sarcophagidae, considering gender and geographical distribution, and compare the resulting profiles with the other species of the families Calliphoridae, Muscidae and Stratiomyidae. The extraction of CHs were made in triplicate using ten female and/or ten males for every species collected in the field. The methodology used for the extraction and analysis followed previously described protocol. We used the GC-MS from the Laboratório de Toxicologia of the Fundação Oswaldo Cruz. Only the CHs with chain length ranging from 21 to 40 carbons were identified, since these are the most commonly found in insects. The relative abundance of each peak was obtained by calculating its area in relation to the total area of the sample, and only those with more than 0.5% were identified. Altogether, 213 compounds were identified and classified as n-alkanes, methyl-branched alkanes and alkenes ranging from 22 to 39 carbons in the main chain (CMC). In Sarcophagidae 164 compounds were identified ranging from 22 to 39 CMC and each species had a different number of identified CHs. Oxysarcodexia thornax was the species with the largest number of identified CHs (48 compounds), contrasting with Peckia (Squamatodes) ingens with the fewest (26 compounds). All species of Sarcophagidae presented specific HCs, as Malacophagomyia filamenta, which was the only that presented trimethylalkanes in its profile as well as having the largest number of specific CHs (14), while Oxysarcodexia intona had the lowest amount (2). Some species show sexual dimorphism, as Ravinia belforti, whose males presented 41 and females 32 HCs with more than 0.5% of abundance. The populations of O. thornax from different regions of Brazil showed similar chromatograms, but, as expected, there were fluctuations in the abundance of shared CHs and only a few specific per population. The two dendrograms generated to assess the similarity among the HCs profiles of the species resulted in different relationships. When n-alkanes are taken into account, the dendrogram showed good correlation among the species of Sarcophagidae, but not among Calliphoridae and Muscidae. However, when excluding the n-alkanes from the analysis, the species of Sarcophagidae continued with good correlation, the Calliphoridae and Muscidae were better correlated and Stratiomyidae, as expected, was presented as the most dissimilar species. The results showed promising use of this technique for the identification of species from Sarcophagidae,and other forensic important families , making it possible to establish a chemotaxonomic database of these species.application/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em Biologia AnimalUFRRJBrasilInstituto de Ciências Biológicas e da SaúdeCromatografia GasosaEpicutículaEntomologia ForenseGas ChromatographyEpicuticleForensic EntomologyBiologia GeralQuimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticularesChemotaxonomy of necrophagous insects Calliphoridae, Muscidae, Sarcophagidae and Stratiomyidae (Diptera) of forensic potential using cuticular hydrocarbon profiles.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisAKINO, T. 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Cuticular hydrocarbon composition in pupal exuviae for taxonomic differentiation of six necrophagous flies. Journal of Medical Entomology, v. 44, p. 450-456, 2007. ZHU, G. H.; YE, G. Y.; HU, C.; XU, X. H.; LI, K. Development changes of cuticular hydrocarbons in Chrysomya rufifacies larvae: potential for determining larval age, Medical and Veterinary Entomology, v. 20, p. 438–44, 2006. ZHU, G. H.; XU, X. H.; YU, X. J.; ZHANG, Y.; WANG, J. F. Puparial case hydrocarbons of Chrysomya megacephala as an indicator of the postmortem interval. Forensic Science International, v. 169, p. 1-5, 2007.https://tede.ufrrj.br/retrieve/63018/2015%20-%20Rodrigo%20Rocha%20Barbosa.pdf.jpghttps://tede.ufrrj.br/jspui/handle/jspui/4147Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-11-18T11:42:23Z No. of bitstreams: 1 2015 - Rodrigo Rocha Barbosa.pdf: 2342499 bytes, checksum: 5961e31f57d6ce1b51146fc09bffc1cf (MD5)Made available in DSpace on 2020-11-18T11:42:23Z (GMT). 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dc.title.por.fl_str_mv Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares
dc.title.alternative.eng.fl_str_mv Chemotaxonomy of necrophagous insects Calliphoridae, Muscidae, Sarcophagidae and Stratiomyidae (Diptera) of forensic potential using cuticular hydrocarbon profiles.
title Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares
spellingShingle Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares
Barbosa, Rodrigo Rocha
Cromatografia Gasosa
Epicutícula
Entomologia Forense
Gas Chromatography
Epicuticle
Forensic Entomology
Biologia Geral
title_short Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares
title_full Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares
title_fullStr Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares
title_full_unstemmed Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares
title_sort Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares
author Barbosa, Rodrigo Rocha
author_facet Barbosa, Rodrigo Rocha
author_role author
dc.contributor.author.fl_str_mv Barbosa, Rodrigo Rocha
dc.contributor.advisor1.fl_str_mv Queiroz, Margareth Maria de Carvalho
dc.contributor.advisor1ID.fl_str_mv 154.256.762-72
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9360088536183208
dc.contributor.advisor-co1.fl_str_mv Braga, Marina Vianna
dc.contributor.referee1.fl_str_mv Nunes, Antônio José Mayhé
dc.contributor.referee2.fl_str_mv Costa, Janyra Oliveira
dc.contributor.referee3.fl_str_mv Thyssen, Patrícia Jaqueline
dc.contributor.referee4.fl_str_mv Mello, Rubens Pinto de
dc.contributor.authorID.fl_str_mv 092.067.357-05
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2236069210294286
contributor_str_mv Queiroz, Margareth Maria de Carvalho
Braga, Marina Vianna
Nunes, Antônio José Mayhé
Costa, Janyra Oliveira
Thyssen, Patrícia Jaqueline
Mello, Rubens Pinto de
dc.subject.por.fl_str_mv Cromatografia Gasosa
Epicutícula
Entomologia Forense
topic Cromatografia Gasosa
Epicutícula
Entomologia Forense
Gas Chromatography
Epicuticle
Forensic Entomology
Biologia Geral
dc.subject.eng.fl_str_mv Gas Chromatography
Epicuticle
Forensic Entomology
dc.subject.cnpq.fl_str_mv Biologia Geral
description A quimiotaxonomia baseada em perfis de hidrocarbonetos cuticulares (HCs) é uma ferramenta taxonômica que tem sido utilizada em insetos desde a década de 70 e sua utilização é baseada em sua espécie-especificidade. Em Diptera, estudos importantes têm sido publicados demonstrando que HCs podem ser utilizados em quimiotaxonomia, sendo úteis inclusive para distinção de espécies crípticas. A família Sarcophagidae possui espécies de importância forense, que são pioneiras na degradação cadavérica e aquelas dos gêneros Peckia Robineau-Desvoidy, 1830 e Oxysarcodexia Townsend, 1917 são encontradas frequentemente em carcaças de animais e cadáveres humanos. Este estudo teve como objetivo descrever, através da cromatografia gasosa acoplada à espectrometria de massas (CG-EM), os perfis dos HCs dos adultos de nove espécies de Sarcophagidae, considerando o sexo e a distribuição geográfica, além de comparar os perfis resultantes com os de outras espécies das famílias Calliphoridae, Muscidae e Stratiomyidae. As extrações dos HCs foram feitas em triplicata usando dez fêmeas e/ou dez machos para cada espécie coletadas em campo. A metodologia de extração e análise seguiu os protocolos anteriormente descritos. Utilizamos o CG-EM do Setor de Agrotóxico do Laboratório de Toxicologia da Fundação Oswaldo Cruz. Foram identificados apenas os HCs com comprimento de cadeia entre 21 e 40 carbonos, por serem os mais comumente encontrados nos insetos. A abundância relativa de cada pico foi obtida calculando sua área em relação à área total da amostra, sendo identificados apenas aqueles representando mais de 0,5%. Ao todo foram identificados 213 compostos classificados como n-alcanos, alcanos metil-ramificados e alcenos entre 22 e 39 carbonos na cadeia principal (CCP). Nos Sarcophagidae foram identificados 164 compostos variando entre 22 e 39 CCP e cada espécie apresentou um número diferente de HCs identificados. Oxysarcodexia thornax foi a espécie com maior quantidade de HCs identificados (48 compostos), contrastando com Peckia (Squamatodes) ingens que teve a menor (26 compostos). Todas as espécies de Sarcophagidae mostraram HCs específicos, como Malacophagomyia filamenta que foi a única que apresentou trimetilalcanos em sua composição, além de ser a com maior quantidade de HCs específicos (14), enquanto que Oxysarcodexia intona apresentou a menor quantidade deles (2). Algumas espécies apresentaram dimorfismo sexual, exemplificado por Ravinia belforti, cujos machos obtiveram 41 HCs com mais de 0,5% de abundância e as fêmeas 32. As populações de O. thornax de diferentes regiões do Brasil apresentaram cromatogramas semelhantes, mas, como esperado, houve oscilações nas abundâncias do HCs compartilhados e alguns poucos específicos por população. Os dois dendrogramas gerados para avaliar a similaridade entre os perfis de HCs das espécies resultaram em diferentes relações. Com a inclusão dos n-alcanos, o dendrograma mostrou boa correlação entre as espécies de Sarcophagidae, mas não entre os Calliphoridae e os Muscidae. No entanto, ao excluir os n-alcanos da análise, as espécies de Sarcophagidae continuaram com boa correlação, as de Calliphoridae e Muscidae ficaram melhor correlacionadas e Stratiomyidae, como esperado, se apresentou como a espécie mais dissimilar. Os resultados se mostraram promissores na utilização desta técnica na identificação de espécies de Sarcophagidae e de outras famílias de importância forense, bem como tornou possível a montagem de um banco de dados com informações acerca da quimiotaxonomia dessas espécies.
publishDate 2015
dc.date.issued.fl_str_mv 2015-03-25
dc.date.accessioned.fl_str_mv 2023-12-21T18:35:52Z
dc.date.available.fl_str_mv 2023-12-21T18:35:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Rodrigo Rocha. Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares. 2015. 97 f. Tese (Doutorado em Biologia Animal) - Recursos Florestais e Engenharia Florestal Universidade Federal Rural do Rio de Janeiro, Seropédica, 2015.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/9162
identifier_str_mv BARBOSA, Rodrigo Rocha. Quimiotaxonomia de insetos necrófagos Calliphoridae, Muscidae, Sarcophagidae e Stratiomyidae (Diptera) de potencial forense utilizando perfis de hidrocarbonetos cuticulares. 2015. 97 f. Tese (Doutorado em Biologia Animal) - Recursos Florestais e Engenharia Florestal Universidade Federal Rural do Rio de Janeiro, Seropédica, 2015.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/9162
dc.language.iso.fl_str_mv por
language por
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