“Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.

Detalhes bibliográficos
Autor(a) principal: Berriel, Lidiane Tavares Duarte
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/12013
Resumo: São poucos os criatórios industriais de pato doméstico no Brasil, pois para a população nacional, carne e ovos desta ave ainda são considerados restritos. As criações de subsistência são comuns no país e o comércio de aves vivas, abatidas, ovos e carne ocorrem principalmente entre os pequenos produtores, casas comerciais e feiras livres. Não existe registros ou conhecimento das condições higiênico sanitárias onde essas aves são criadas. A classe das aves está suscetível a se infectarem por três espécies de Cryptosporidium (Cryptosporidium meleagridis, C. baileyi e C. galli), além destas 13 genótipos. O trabalho teve como objetivos: diagnosticar microscopicamente a presença de Cryptosporidium spp. no pato doméstico da família Anatidae (Cairina moschata), provenientes de duas criações rústicas localizadas no Rio de Janeiro; realizar a caracterização genotípica seguida do sequenciamento e análises filogenéticas, comparando com as sequencias obtidas com aquelas sequências depositadas no GenBank. Como metodologia, amostras fecais de patos domésticos (Cairina moschata) foram colhidas em duas criações rústicas (Área A e B), totalizando 118 amostras, sendo 60 amostras da área A e 58 da área B. Os animais da coleta eram aves jovens que não ultrapassaram 30 dias de vida. Inicialmente foi realizada a pesquisa de oocistos Cryptosporidium spp., através da técnica de centrifugação e flutuação em solução saturada de açúcar. No diagnóstico microscópico, de um total de 118 amostras analisadas 20 apresentaram positividade para Cryptosporidium (118/16,95%), sendo estas pertencentes a área A (60/ 33,33%). Para a realização da extração de DNA, foram selecionadas aquelas que apresentavam mais de 20 oocistos de Cryptosporidium spp. por campo microscópico, seguida de metodologias para o diagnostico molecular tendo como alvo o 18S, que posteriormente foram sequenciadas e análises filogenéticas foram feitas. Neste estudo, foi diagnosticada a espécie Cryptosporidium baileyi, apresentando-se mais estreitamente relacionado às espécies gástricas, sendo uma espécie de importância em saúde animal
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spelling Berriel, Lidiane Tavares DuarteBomfim, Teresa Cristina Bergamo do609.928.087-15https://orcid.org/0000-0002-6741-0202http://lattes.cnpq.br/6299581965425007Bomfim, Teresa Cristina Bergamo do609.928.087-15https://orcid.org/0000-0002-6741-0202http://lattes.cnpq.br/6299581965425007Sudré, Adriana Pittellahttps://orcid.org/0000-0002-0386-6278http://lattes.cnpq.br/1820344065898657Figueiredo, Beatriz Brener dehttps://orcid.org/0000-0002-2699-6884http://lattes.cnpq.br/2863484783864410105.064.327-50http://lattes.cnpq.br/45869235643977262023-12-22T02:00:44Z2023-12-22T02:00:44Z2017-03-30BERRIEL, Lidiane Tavares Duarte. “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”. 2017. 77 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro ,Rio de Janeiro,Seropédica, 2017.https://rima.ufrrj.br/jspui/handle/20.500.14407/12013São poucos os criatórios industriais de pato doméstico no Brasil, pois para a população nacional, carne e ovos desta ave ainda são considerados restritos. As criações de subsistência são comuns no país e o comércio de aves vivas, abatidas, ovos e carne ocorrem principalmente entre os pequenos produtores, casas comerciais e feiras livres. Não existe registros ou conhecimento das condições higiênico sanitárias onde essas aves são criadas. A classe das aves está suscetível a se infectarem por três espécies de Cryptosporidium (Cryptosporidium meleagridis, C. baileyi e C. galli), além destas 13 genótipos. O trabalho teve como objetivos: diagnosticar microscopicamente a presença de Cryptosporidium spp. no pato doméstico da família Anatidae (Cairina moschata), provenientes de duas criações rústicas localizadas no Rio de Janeiro; realizar a caracterização genotípica seguida do sequenciamento e análises filogenéticas, comparando com as sequencias obtidas com aquelas sequências depositadas no GenBank. Como metodologia, amostras fecais de patos domésticos (Cairina moschata) foram colhidas em duas criações rústicas (Área A e B), totalizando 118 amostras, sendo 60 amostras da área A e 58 da área B. Os animais da coleta eram aves jovens que não ultrapassaram 30 dias de vida. Inicialmente foi realizada a pesquisa de oocistos Cryptosporidium spp., através da técnica de centrifugação e flutuação em solução saturada de açúcar. No diagnóstico microscópico, de um total de 118 amostras analisadas 20 apresentaram positividade para Cryptosporidium (118/16,95%), sendo estas pertencentes a área A (60/ 33,33%). Para a realização da extração de DNA, foram selecionadas aquelas que apresentavam mais de 20 oocistos de Cryptosporidium spp. por campo microscópico, seguida de metodologias para o diagnostico molecular tendo como alvo o 18S, que posteriormente foram sequenciadas e análises filogenéticas foram feitas. Neste estudo, foi diagnosticada a espécie Cryptosporidium baileyi, apresentando-se mais estreitamente relacionado às espécies gástricas, sendo uma espécie de importância em saúde animalCNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoThere are few industrial duck farms in Brazil, because for the national population, the meat and eggs of this bird are still considered restricted. Subsistence creations are common in the country, and trade in live, slaughtered poultry, eggs and meat mainly occurs among small producers, commercial houses and free markets. There are no records or knowledge of the hygienic sanitary conditions where these birds are created. The class of birds is susceptible to infection by three species of Cryptosporidium (Cryptosporidium meleagridis, C. baileyi and C. galli), in addition to these 13 genotypes. The objective of this study was to diagnose the presence of Cryptosporidium spp. in the domestic duck of the family Anatidae (Cairina moschata), from two rustic creations located in Rio de Janeiro; Carry out the genotypic characterization followed by sequencing and phylogenetic analyzes, comparing with the sequences obtained with those sequences deposited in GenBank. As a methodology, fecal samples of domestic ducks (Cairina moschata) were collected in two rustic creations (Area A and B), totaling 118 samples, 60 samples from area A and 58 from area B. The animals of the collection were young birds that did not exceed 30 days of life. Initially the research of oocysts Cryptosporidium spp. was carried out, through the technique of centrifugation and flotation in saturated solution of sugar. In the microscopic diagnosis, 20 samples from area A showed positivity (118 / 16.95%). Of these, we selected those that had more than 20 oocysts of Cryptosporidium spp. By microscopic field for the extraction of DNA, followed by molecular diagnostic methodologies targeting 18S, which were later, sequenced and phylogenetic analyzes were performed. In this study, the species Cryptosporidium baileyi, was diagnosed, being more closely related to the gastric species, being a species of importance in animal healthapplication/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em Ciências VeterináriasUFRRJBrasilInstituto de VeterináriaAnseriformesCriações rústicasDiagnóstico molecularCryptosporidium baileyiAnseriformesRustic creationsMolecular diagnosisCryptosporidium baileyiMedicina Veterinária“Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.“Importance of domestic ducks of the family Anatidae (Cairina moschata) of rustic creations parasitized with Cryptosporidium"info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisABASSI H.; WYERS, M.; CABARETE, J.; NACIRI, M. Rapid detection and quantification of Cryptosporidium baileyi oocysts in feces and organs of chickens using a microscopic slide flotation method. 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Applied and Environmental Microbiology, v. 74, n. 21, p. 6495-6504, 2008. YODER, J. S.; WALLACE, R. M.; COLLIER, S. A. et al. Cryptosporidiosis surveillance - United States, 2009-2010. Surveill. Summ. v.61, p.1-12, 2012. ZAHEDI, A.; PAPARINI, A.; JIAN, F.; ROBERTSON, I.; RYAN, U. Public health significance of zoonotic Cryptosporidium species in wildlife: Critical insights into better drinking water management. International Journal for Parasitology Parasites and Wildlife. v. 5, n. 1, p.88-109, 2016. ZHOU, L.; KASSA, H.; TISCHLER, M. XIAO, L. Host-Adapted Cryptosporidium spp. in Canada Geese (Branta canadensis). American Society for Microbiology, v.70, n.7, p. 4211-4215, 2004. ZIEGLER, P. E.; WADE, S. E.; SCHAAF, S. L.; et al. Prevalence of Cryptosporidium species in wildlife populations within a watershed landscape in southeastern New York State. Veterinary Parasitology, v. 43, n. 2, p.586–596, 2007. 62 ZYLAN, K.; BAILEN, T.; SMITH, H. V.; et al. An outbreak of cryptosporidiosis in a collection of Stone curlews (Burhinus oedicnemus) in Dubai. Avian Pathology, v.37, n. 5, p. 521-526, 2008https://tede.ufrrj.br/retrieve/70168/2017%20-%20Lidiane%20Tavares%20Duarte%20Berriel.pdf.jpghttps://tede.ufrrj.br/jspui/handle/jspui/5853Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2022-08-08T13:48:39Z No. of bitstreams: 1 2017 - Lidiane Tavares Duarte Berriel.pdf: 2719610 bytes, checksum: 072032a149ce9c09d8e09a182c4a12cc (MD5)Made available in DSpace on 2022-08-08T13:48:39Z (GMT). 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dc.title.por.fl_str_mv “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.
dc.title.alternative.eng.fl_str_mv “Importance of domestic ducks of the family Anatidae (Cairina moschata) of rustic creations parasitized with Cryptosporidium"
title “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.
spellingShingle “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.
Berriel, Lidiane Tavares Duarte
Anseriformes
Criações rústicas
Diagnóstico molecular
Cryptosporidium baileyi
Anseriformes
Rustic creations
Molecular diagnosis
Cryptosporidium baileyi
Medicina Veterinária
title_short “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.
title_full “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.
title_fullStr “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.
title_full_unstemmed “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.
title_sort “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”.
author Berriel, Lidiane Tavares Duarte
author_facet Berriel, Lidiane Tavares Duarte
author_role author
dc.contributor.author.fl_str_mv Berriel, Lidiane Tavares Duarte
dc.contributor.advisor1.fl_str_mv Bomfim, Teresa Cristina Bergamo do
dc.contributor.advisor1ID.fl_str_mv 609.928.087-15
https://orcid.org/0000-0002-6741-0202
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6299581965425007
dc.contributor.referee1.fl_str_mv Bomfim, Teresa Cristina Bergamo do
dc.contributor.referee1ID.fl_str_mv 609.928.087-15
https://orcid.org/0000-0002-6741-0202
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/6299581965425007
dc.contributor.referee2.fl_str_mv Sudré, Adriana Pittella
dc.contributor.referee2ID.fl_str_mv https://orcid.org/0000-0002-0386-6278
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1820344065898657
dc.contributor.referee3.fl_str_mv Figueiredo, Beatriz Brener de
dc.contributor.referee3ID.fl_str_mv https://orcid.org/0000-0002-2699-6884
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/2863484783864410
dc.contributor.authorID.fl_str_mv 105.064.327-50
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4586923564397726
contributor_str_mv Bomfim, Teresa Cristina Bergamo do
Bomfim, Teresa Cristina Bergamo do
Sudré, Adriana Pittella
Figueiredo, Beatriz Brener de
dc.subject.por.fl_str_mv Anseriformes
Criações rústicas
Diagnóstico molecular
Cryptosporidium baileyi
topic Anseriformes
Criações rústicas
Diagnóstico molecular
Cryptosporidium baileyi
Anseriformes
Rustic creations
Molecular diagnosis
Cryptosporidium baileyi
Medicina Veterinária
dc.subject.eng.fl_str_mv Anseriformes
Rustic creations
Molecular diagnosis
Cryptosporidium baileyi
dc.subject.cnpq.fl_str_mv Medicina Veterinária
description São poucos os criatórios industriais de pato doméstico no Brasil, pois para a população nacional, carne e ovos desta ave ainda são considerados restritos. As criações de subsistência são comuns no país e o comércio de aves vivas, abatidas, ovos e carne ocorrem principalmente entre os pequenos produtores, casas comerciais e feiras livres. Não existe registros ou conhecimento das condições higiênico sanitárias onde essas aves são criadas. A classe das aves está suscetível a se infectarem por três espécies de Cryptosporidium (Cryptosporidium meleagridis, C. baileyi e C. galli), além destas 13 genótipos. O trabalho teve como objetivos: diagnosticar microscopicamente a presença de Cryptosporidium spp. no pato doméstico da família Anatidae (Cairina moschata), provenientes de duas criações rústicas localizadas no Rio de Janeiro; realizar a caracterização genotípica seguida do sequenciamento e análises filogenéticas, comparando com as sequencias obtidas com aquelas sequências depositadas no GenBank. Como metodologia, amostras fecais de patos domésticos (Cairina moschata) foram colhidas em duas criações rústicas (Área A e B), totalizando 118 amostras, sendo 60 amostras da área A e 58 da área B. Os animais da coleta eram aves jovens que não ultrapassaram 30 dias de vida. Inicialmente foi realizada a pesquisa de oocistos Cryptosporidium spp., através da técnica de centrifugação e flutuação em solução saturada de açúcar. No diagnóstico microscópico, de um total de 118 amostras analisadas 20 apresentaram positividade para Cryptosporidium (118/16,95%), sendo estas pertencentes a área A (60/ 33,33%). Para a realização da extração de DNA, foram selecionadas aquelas que apresentavam mais de 20 oocistos de Cryptosporidium spp. por campo microscópico, seguida de metodologias para o diagnostico molecular tendo como alvo o 18S, que posteriormente foram sequenciadas e análises filogenéticas foram feitas. Neste estudo, foi diagnosticada a espécie Cryptosporidium baileyi, apresentando-se mais estreitamente relacionado às espécies gástricas, sendo uma espécie de importância em saúde animal
publishDate 2017
dc.date.issued.fl_str_mv 2017-03-30
dc.date.accessioned.fl_str_mv 2023-12-22T02:00:44Z
dc.date.available.fl_str_mv 2023-12-22T02:00:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BERRIEL, Lidiane Tavares Duarte. “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”. 2017. 77 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro ,Rio de Janeiro,Seropédica, 2017.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/12013
identifier_str_mv BERRIEL, Lidiane Tavares Duarte. “Importância de patos doméstico da família Anatidae (Cairina moschata) de criações rústicas parasitados com Cryptosporidium”. 2017. 77 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro ,Rio de Janeiro,Seropédica, 2017.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/12013
dc.language.iso.fl_str_mv por
language por
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