Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas

Dias, Albiane Carvalho

Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas

Detalhes bibliográficos
Autor(a) principal:	Dias, Albiane Carvalho
Data de Publicação:	2021
Tipo de documento:	Tese
Idioma:	por
Título da fonte:	Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo:	https://rima.ufrrj.br/jspui/handle/20.500.14407/9017
Resumo:	O milho (Zea mays L.) é um dos principais cereais produzidos no mundo. A sua inoculação com bactérias diazotróficas descritas como promotoras de crescimento de plantas pode reduzir a demanda por fertilizantes nitrogenados e ao mesmo tempo os gastos associados a essa prática. Este estudo investigou como a inoculação das bactérias diazotróficas Azospirillum brasilense, Azospirillum baldaniorum e Herbaspirillum seropedicae em plantas de milho influenciaria a modulação da arquitetura da raiz e os parâmetros associados à taxa de absorção de nutrientes, especialmente de nitrogênio (N), sob diferentes condições de disponibilidade de N. Foram desenvolvidos seis experimentos, quatro em sistema hidropônico e dois em substrato estéril (areia+vermiculita) em casa de vegetação, a fim de verificar o metabolismo e a expressão de genes envolvidos na absorção e assimilação de N. Para isso, foram avaliadas nos tecidos vegetais as frações nitrogenadas, açúcares solúveis, a atividade das enzimas nitrato redutase e glutamina sintetase, a expressão gênica de isoformas de bombas de prótons (ZmHA2 e ZmHA4), transportadores de alta afinidade de nitrato (NO3-) (ZmNRT2.3, ZmNRT2.5 e ZmNRT3.1A) e das enzimas de redução e assimilação de N (ZmNR1, ZmNR2, ZmGS1.1, ZmGS1.5, ZmGS2 e ZmNADH-GOGAT). Parâmetros como acúmulo de biomassa, N total, eficiência do uso de N (EUN), área foliar e índice de clorofila também foram avaliados. A inoculação com as estirpes de Azospirillum baldaniorum (Ab)-Sp245 e Herbaspirillum seropedicae (Hs)-ZAE94 promoveram as maiores mudanças na arquitetura radicular, com melhora em todos os parâmetros avaliados, o que estava de acordo com o aumento da massa seca e acúmulo de N, mas dependente da data de amostragem e do ensaio. Incrementos no acúmulo de biomassa e N total em até 150 e 180%, respectivamente, foram encontrados no tratamento inoculado com Ab-Sp245 em relação ao controle. A inoculação com ambas as estirpes também promoveu incrementos superiores a 90% para comprimento, volume e área radicular. Os parâmetros avaliados melhoraram em função da maior disponibilidade de N. A Ab-Sp245 foi mais efetiva na maior absorção de macronutrientes, com maior velocidade de absorção de NO3- e proporcionou uma maior EUN sob baixo N. As mudanças provocadas pela inoculação no perfil de expressão gênica foram menos consistentes, somente após 2 h da indução do sistema de transporte de NO3- a Hs-ZAE94 aumentou a expressão de dois transportadores de alta afinidade de NO3- (ZmNRT2.3 e ZmNRT2.5) e duas isoformas de glutamina sintetase (ZmGS1.1 e ZmGS2) quando comparado ao controle, mas as encontradas no conteúdo de metabólitos solúveis e atividade enzimática indicaram melhoria no processo de assimilação de N nas plantas inoculadas. As mudanças na arquitetura radicular e a manutenção da absorção de N por unidade de área da raiz através da inoculação com Ab-Sp245 foi provavelmente o efeito principal.

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spelling	Dias, Albiane CarvalhoReis, Veronica Massena631.052.847-53http://lattes.cnpq.br/9099587982889283Santos, Leandro AzevedoReis, Veronica MassenaAlves, Bruno José RodriguesCoelho, Irene da SilvaVidal, Marcia SoaresAlves, Gabriela Cavalcanti142.182.367-55http://lattes.cnpq.br/78756275435518102023-12-21T18:33:34Z2023-12-21T18:33:34Z2021-11-30DIAS, Albiane Carvalho. Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas. 2021. 134 f. Tese (Doutorado em Agronomia, Ciência do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica - RJ, 2021.https://rima.ufrrj.br/jspui/handle/20.500.14407/9017O milho (Zea mays L.) é um dos principais cereais produzidos no mundo. A sua inoculação com bactérias diazotróficas descritas como promotoras de crescimento de plantas pode reduzir a demanda por fertilizantes nitrogenados e ao mesmo tempo os gastos associados a essa prática. Este estudo investigou como a inoculação das bactérias diazotróficas Azospirillum brasilense, Azospirillum baldaniorum e Herbaspirillum seropedicae em plantas de milho influenciaria a modulação da arquitetura da raiz e os parâmetros associados à taxa de absorção de nutrientes, especialmente de nitrogênio (N), sob diferentes condições de disponibilidade de N. Foram desenvolvidos seis experimentos, quatro em sistema hidropônico e dois em substrato estéril (areia+vermiculita) em casa de vegetação, a fim de verificar o metabolismo e a expressão de genes envolvidos na absorção e assimilação de N. Para isso, foram avaliadas nos tecidos vegetais as frações nitrogenadas, açúcares solúveis, a atividade das enzimas nitrato redutase e glutamina sintetase, a expressão gênica de isoformas de bombas de prótons (ZmHA2 e ZmHA4), transportadores de alta afinidade de nitrato (NO3-) (ZmNRT2.3, ZmNRT2.5 e ZmNRT3.1A) e das enzimas de redução e assimilação de N (ZmNR1, ZmNR2, ZmGS1.1, ZmGS1.5, ZmGS2 e ZmNADH-GOGAT). Parâmetros como acúmulo de biomassa, N total, eficiência do uso de N (EUN), área foliar e índice de clorofila também foram avaliados. A inoculação com as estirpes de Azospirillum baldaniorum (Ab)-Sp245 e Herbaspirillum seropedicae (Hs)-ZAE94 promoveram as maiores mudanças na arquitetura radicular, com melhora em todos os parâmetros avaliados, o que estava de acordo com o aumento da massa seca e acúmulo de N, mas dependente da data de amostragem e do ensaio. Incrementos no acúmulo de biomassa e N total em até 150 e 180%, respectivamente, foram encontrados no tratamento inoculado com Ab-Sp245 em relação ao controle. A inoculação com ambas as estirpes também promoveu incrementos superiores a 90% para comprimento, volume e área radicular. Os parâmetros avaliados melhoraram em função da maior disponibilidade de N. A Ab-Sp245 foi mais efetiva na maior absorção de macronutrientes, com maior velocidade de absorção de NO3- e proporcionou uma maior EUN sob baixo N. As mudanças provocadas pela inoculação no perfil de expressão gênica foram menos consistentes, somente após 2 h da indução do sistema de transporte de NO3- a Hs-ZAE94 aumentou a expressão de dois transportadores de alta afinidade de NO3- (ZmNRT2.3 e ZmNRT2.5) e duas isoformas de glutamina sintetase (ZmGS1.1 e ZmGS2) quando comparado ao controle, mas as encontradas no conteúdo de metabólitos solúveis e atividade enzimática indicaram melhoria no processo de assimilação de N nas plantas inoculadas. As mudanças na arquitetura radicular e a manutenção da absorção de N por unidade de área da raiz através da inoculação com Ab-Sp245 foi provavelmente o efeito principal.CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorCNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPERJ - Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroEMBRAPA - Empresa Brasileira de Pesquisa AgropecuáriaMaize (Zea mays L) is one of the world’s leading produced cereals. Its inoculation with diazotrophic bacteria described as plant growth-promoters can reduce the demand for nitrogen fertilizers and at the same time the costs associated with this practice. This study investigated how inoculation of the diazotrophic bacteria Azospirillum brasilense, Azospirillum baldaniorum and Herbaspirillum seropedicae in maize plants would influence the modulation of root architecture and the parameters associated with the absorption rate of nutrients, especially nitrogen (N), under different conditions of availability of N. Experiments were developed in a hydroponic system and sterile substrate (sand+vermiculite) in a greenhouse, in order to verify the metabolism and expression of genes involved in the absorption and assimilation of N. For this purpose, nitrogen fractions, the activity of the enzymes nitrate reductase and glutamine synthetase, the gene expression of proton pump isoforms (ZmHA2 and ZmHA4), high-affinity nitrate transporters (NO3-) (ZmNRT2.3, ZmNRT2.5 and ZmNRT3.1A) and N-reduction and assimilation enzymes (ZmNR1, ZmNR2, ZmGS1.1, ZmGS1.5, ZmGS2 and ZmNADH-GOGAT) were evaluated. Parameters such as biomass accumulation, total N, N use efficiency (NUE), leaf area and chlorophyll index were also evaluated. The inoculation with A. baldaniorum (Ab)-Sp245 and H. seropedicae (Hs)-ZAE94 strains promoted the greatest changes in root architecture, with improvement in all parameters evaluated, which was in agreement with the increase in dry mass and accumulation of N, but they were dependent on the date of sampling and experiment. Increases in the accumulation of biomass and total N of up to 150 and 180%, respectively, were found in the treatment inoculated with Ab-Sp245 in relation to the control. Inoculation with both strains also promoted increments greater than 90% for length, volume and root área. The parameters improved due to greater availability of N. The Ab-Sp245 was more effective in the greater absorption of macronutrients, with higher speed of absorption of NO3- and provided a greater NUE under low N. The changes caused by inoculation in the gene expression profile were less consistent, only after 2 h of induction of the NO3- transport system did Hs-ZAE94 increase the expression of two high-affinity NO3- transporters (ZmNRT2.3 and ZmNRT2.5) and two isoforms of glutamine synthetase (ZmGS1.1 and ZmGS2) When compared to the control, but those found in the content of soluble metabolites and enzymatic activity indicated an improvement in the N assimilation process in the inoculated plants. The shift in root architecture and the maintenance of N uptake per unit of root area through inoculation with Ab-Sp245 was probably the main effect.application/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em Agronomia - Ciência do SoloUFRRJBrasilInstituto de AgronomiaAzospirillum baldaniorumBactéria promotora de crescimento de plantaHerbaspirillum seropedicaeNitratoZea mays L.Plant growth-promoting bacteriaNitrateAgronomiaMetabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficasNitrogen metabolism in maize plants inoculated with two genera of diazotrophic bacteriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisADESEMOYER, A. O.; KLOEPPER, J. W. Plant-microbes interactions in enhanced fertilizeruse efficiency. Applied Microbiology and Biotechnlogy, v. 85, n. 1, p. 1-12, 2009. ADESEMOYE, A.O.; TORBERT, H.A.; KLOEPPER J.W. 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dc.title.por.fl_str_mv	Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas
dc.title.alternative.eng.fl_str_mv	Nitrogen metabolism in maize plants inoculated with two genera of diazotrophic bacteria
title	Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas
spellingShingle	Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas Dias, Albiane Carvalho Azospirillum baldaniorum Bactéria promotora de crescimento de planta Herbaspirillum seropedicae Nitrato Zea mays L. Plant growth-promoting bacteria Nitrate Agronomia
title_short	Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas
title_full	Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas
title_fullStr	Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas
title_full_unstemmed	Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas
title_sort	Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas
author	Dias, Albiane Carvalho
author_facet	Dias, Albiane Carvalho
author_role	author
dc.contributor.author.fl_str_mv	Dias, Albiane Carvalho
dc.contributor.advisor1.fl_str_mv	Reis, Veronica Massena
dc.contributor.advisor1ID.fl_str_mv	631.052.847-53
dc.contributor.advisor1Lattes.fl_str_mv	http://lattes.cnpq.br/9099587982889283
dc.contributor.advisor-co1.fl_str_mv	Santos, Leandro Azevedo
dc.contributor.referee1.fl_str_mv	Reis, Veronica Massena
dc.contributor.referee2.fl_str_mv	Alves, Bruno José Rodrigues
dc.contributor.referee3.fl_str_mv	Coelho, Irene da Silva
dc.contributor.referee4.fl_str_mv	Vidal, Marcia Soares
dc.contributor.referee5.fl_str_mv	Alves, Gabriela Cavalcanti
dc.contributor.authorID.fl_str_mv	142.182.367-55
dc.contributor.authorLattes.fl_str_mv	http://lattes.cnpq.br/7875627543551810
contributor_str_mv	Reis, Veronica Massena Santos, Leandro Azevedo Reis, Veronica Massena Alves, Bruno José Rodrigues Coelho, Irene da Silva Vidal, Marcia Soares Alves, Gabriela Cavalcanti
dc.subject.por.fl_str_mv	Azospirillum baldaniorum Bactéria promotora de crescimento de planta Herbaspirillum seropedicae Nitrato Zea mays L.
topic	Azospirillum baldaniorum Bactéria promotora de crescimento de planta Herbaspirillum seropedicae Nitrato Zea mays L. Plant growth-promoting bacteria Nitrate Agronomia
dc.subject.eng.fl_str_mv	Plant growth-promoting bacteria Nitrate
dc.subject.cnpq.fl_str_mv	Agronomia
description	O milho (Zea mays L.) é um dos principais cereais produzidos no mundo. A sua inoculação com bactérias diazotróficas descritas como promotoras de crescimento de plantas pode reduzir a demanda por fertilizantes nitrogenados e ao mesmo tempo os gastos associados a essa prática. Este estudo investigou como a inoculação das bactérias diazotróficas Azospirillum brasilense, Azospirillum baldaniorum e Herbaspirillum seropedicae em plantas de milho influenciaria a modulação da arquitetura da raiz e os parâmetros associados à taxa de absorção de nutrientes, especialmente de nitrogênio (N), sob diferentes condições de disponibilidade de N. Foram desenvolvidos seis experimentos, quatro em sistema hidropônico e dois em substrato estéril (areia+vermiculita) em casa de vegetação, a fim de verificar o metabolismo e a expressão de genes envolvidos na absorção e assimilação de N. Para isso, foram avaliadas nos tecidos vegetais as frações nitrogenadas, açúcares solúveis, a atividade das enzimas nitrato redutase e glutamina sintetase, a expressão gênica de isoformas de bombas de prótons (ZmHA2 e ZmHA4), transportadores de alta afinidade de nitrato (NO3-) (ZmNRT2.3, ZmNRT2.5 e ZmNRT3.1A) e das enzimas de redução e assimilação de N (ZmNR1, ZmNR2, ZmGS1.1, ZmGS1.5, ZmGS2 e ZmNADH-GOGAT). Parâmetros como acúmulo de biomassa, N total, eficiência do uso de N (EUN), área foliar e índice de clorofila também foram avaliados. A inoculação com as estirpes de Azospirillum baldaniorum (Ab)-Sp245 e Herbaspirillum seropedicae (Hs)-ZAE94 promoveram as maiores mudanças na arquitetura radicular, com melhora em todos os parâmetros avaliados, o que estava de acordo com o aumento da massa seca e acúmulo de N, mas dependente da data de amostragem e do ensaio. Incrementos no acúmulo de biomassa e N total em até 150 e 180%, respectivamente, foram encontrados no tratamento inoculado com Ab-Sp245 em relação ao controle. A inoculação com ambas as estirpes também promoveu incrementos superiores a 90% para comprimento, volume e área radicular. Os parâmetros avaliados melhoraram em função da maior disponibilidade de N. A Ab-Sp245 foi mais efetiva na maior absorção de macronutrientes, com maior velocidade de absorção de NO3- e proporcionou uma maior EUN sob baixo N. As mudanças provocadas pela inoculação no perfil de expressão gênica foram menos consistentes, somente após 2 h da indução do sistema de transporte de NO3- a Hs-ZAE94 aumentou a expressão de dois transportadores de alta afinidade de NO3- (ZmNRT2.3 e ZmNRT2.5) e duas isoformas de glutamina sintetase (ZmGS1.1 e ZmGS2) quando comparado ao controle, mas as encontradas no conteúdo de metabólitos solúveis e atividade enzimática indicaram melhoria no processo de assimilação de N nas plantas inoculadas. As mudanças na arquitetura radicular e a manutenção da absorção de N por unidade de área da raiz através da inoculação com Ab-Sp245 foi provavelmente o efeito principal.
publishDate	2021
dc.date.issued.fl_str_mv	2021-11-30
dc.date.accessioned.fl_str_mv	2023-12-21T18:33:34Z
dc.date.available.fl_str_mv	2023-12-21T18:33:34Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/doctoralThesis
format	doctoralThesis
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	DIAS, Albiane Carvalho. Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas. 2021. 134 f. Tese (Doutorado em Agronomia, Ciência do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica - RJ, 2021.
dc.identifier.uri.fl_str_mv	https://rima.ufrrj.br/jspui/handle/20.500.14407/9017
identifier_str_mv	DIAS, Albiane Carvalho. Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas. 2021. 134 f. Tese (Doutorado em Agronomia, Ciência do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica - RJ, 2021.
url	https://rima.ufrrj.br/jspui/handle/20.500.14407/9017
dc.language.iso.fl_str_mv	por
language	por
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Metabolismo de nitrogênio em plantas de milho inoculadas com dois gêneros de bactérias diazotróficas

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