Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro

Detalhes bibliográficos
Autor(a) principal: Pribul, Bruno Rocha
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://rima.ufrrj.br/jspui/handle/20.500.14407/11901
Resumo: PRIBUL, Bruno Rocha. Avaliação da Resistência a Vancomicina e do Perfil de Suscetibilidade a Bacteriocinas de Staphylococcus spp Isolados de Mastite Bovina da Região Sul do Estado do Rio de Janeiro. 41p Dissertação (Mestrado em Ciências Veterinárias). Instituto de Veterinária, Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2011. As bactérias do gênero Staphylococcus estão entre as mais implicadas na etiologia das mastites. Uma dificuldade adicional no controle das infecções bacterianas por este gênero é representada por sua freqüente resistência aos antimicrobianos. Os glicopeptídeos, como a vancomicina, aparecem como uma alternativa terapêutica devido ao crescente aumento de cepas estafilocócicas resistentes aos betalactâmicos, que são os fármacos de eleição nestas infecções. Desse modo, a resistência aos glicopeptídeos representa uma ameaça ao futuro da terapia antimicrobiana em humanos e animais. O presente trabalho buscou avaliar o perfil de resistência à vancomicina em isolados de Staphylococcus spp. oriundos de amostras de leite de vacas com mastite. Todos os 150 isolados estudados (50 Staphylococcus aureus, 50 Staphylococcus intermedius e 50 Staphylococcus spp. coagulase negativos), foram avaliados através dos testes de difusão em disco simples, microdiluição em ágar e detecção dos genes vanA e B. O teste de suscetibilidade antimicrobiana revelou elevada resistência aos beta-lactâmicos para todos os grupos avaliados. No ensaio de difusão em disco, os isolados coagulasepositivos apresentaram um perfil de resistência de 24% a teicoplanina (24/100) e 23% a vancomicina (23/100). No teste de microdiluição em ágar para detecção da CIM, 16% dos isolados apresentaram resistência à vancomicina (16/100). Entre os isolados resistentes no teste de microdiluição em ágar, 56,25% dos isolados (9/16) apresentaram valores de CIM que variaram entre 4-8μg/ml. Os Staphylococcus coagulase-negativos não apresentaram resistência aos glicopeptideos. Os genes de resistência à vancomicina foram detectados em 37,5% dos isolados vancomicina-resistentes (6/16). Para se buscar uma correlação entre a presença de resistência aos glicopeptideos e a capacidade de formação de biofilme foram realizados os testes de caracterização de crescimento em ágar vermelho congo e detecção de biofilme pelo teste de aderência em microplacas, onde foram detectados 66,66% e 90,00% respectivamente, de isolados produtores de biofilme. A produção de “slime” não apresentou correlação estatística com a resistência aos glicopeptídeos. O perfil de sensibilidade dos isolados de Staphylococcus spp. foi avaliado frente as bacteriocinas produzidas pelos Lactobacillus paracasei, Lactobacillus acidophillus e Lactobacillus fermentum. As bacteriocinas que apresentaram maior capacidade de inibição frente aos Saphylococcus spp. testados foram as produzidas pelos Lactobacillus paracasei
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spelling Pribul, Bruno RochaSouza, Miliane Moreira Soares de1076198732http://lattes.cnpq.br/08652112146186185640401770http://lattes.cnpq.br/41092299053808112023-12-22T01:58:26Z2023-12-22T01:58:26Z2011-02-24PRIBUL, Bruno Rocha. Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro. 2011. 64 f. Dissertação (Programa de Pós-Graduação em Ciências Veterinárias - Universidade Federal Rural do Rio de Janeiro, Seropédica.https://rima.ufrrj.br/jspui/handle/20.500.14407/11901PRIBUL, Bruno Rocha. Avaliação da Resistência a Vancomicina e do Perfil de Suscetibilidade a Bacteriocinas de Staphylococcus spp Isolados de Mastite Bovina da Região Sul do Estado do Rio de Janeiro. 41p Dissertação (Mestrado em Ciências Veterinárias). Instituto de Veterinária, Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2011. As bactérias do gênero Staphylococcus estão entre as mais implicadas na etiologia das mastites. Uma dificuldade adicional no controle das infecções bacterianas por este gênero é representada por sua freqüente resistência aos antimicrobianos. Os glicopeptídeos, como a vancomicina, aparecem como uma alternativa terapêutica devido ao crescente aumento de cepas estafilocócicas resistentes aos betalactâmicos, que são os fármacos de eleição nestas infecções. Desse modo, a resistência aos glicopeptídeos representa uma ameaça ao futuro da terapia antimicrobiana em humanos e animais. O presente trabalho buscou avaliar o perfil de resistência à vancomicina em isolados de Staphylococcus spp. oriundos de amostras de leite de vacas com mastite. Todos os 150 isolados estudados (50 Staphylococcus aureus, 50 Staphylococcus intermedius e 50 Staphylococcus spp. coagulase negativos), foram avaliados através dos testes de difusão em disco simples, microdiluição em ágar e detecção dos genes vanA e B. O teste de suscetibilidade antimicrobiana revelou elevada resistência aos beta-lactâmicos para todos os grupos avaliados. No ensaio de difusão em disco, os isolados coagulasepositivos apresentaram um perfil de resistência de 24% a teicoplanina (24/100) e 23% a vancomicina (23/100). No teste de microdiluição em ágar para detecção da CIM, 16% dos isolados apresentaram resistência à vancomicina (16/100). Entre os isolados resistentes no teste de microdiluição em ágar, 56,25% dos isolados (9/16) apresentaram valores de CIM que variaram entre 4-8μg/ml. Os Staphylococcus coagulase-negativos não apresentaram resistência aos glicopeptideos. Os genes de resistência à vancomicina foram detectados em 37,5% dos isolados vancomicina-resistentes (6/16). Para se buscar uma correlação entre a presença de resistência aos glicopeptideos e a capacidade de formação de biofilme foram realizados os testes de caracterização de crescimento em ágar vermelho congo e detecção de biofilme pelo teste de aderência em microplacas, onde foram detectados 66,66% e 90,00% respectivamente, de isolados produtores de biofilme. A produção de “slime” não apresentou correlação estatística com a resistência aos glicopeptídeos. O perfil de sensibilidade dos isolados de Staphylococcus spp. foi avaliado frente as bacteriocinas produzidas pelos Lactobacillus paracasei, Lactobacillus acidophillus e Lactobacillus fermentum. As bacteriocinas que apresentaram maior capacidade de inibição frente aos Saphylococcus spp. testados foram as produzidas pelos Lactobacillus paracaseiConselho Nacional de Desenvolvimento Científico e Tecnológico - CNPqPRIBUL, Bruno Rocha. Evaluation of Vancomycin Resistance and Susceptibility Profile Bacteriocins of staphylococci isolated from bovine mastitis in Southern State of Rio de Janeiro. 41p Thesis (Master of Veterinary Science). Instituto de Veterinária, Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2011. Bacteria of the genus Staphylococcus are among the most implicated in the etiology of mastitis. An additional difficulty in controlling bacterial infections by this genus is represented by its frequent resistance to antibiotics. The glycopeptides (eg vancomycin) appear as an alternative therapy because of the increasing strains of staphylococci resistant to beta-lactams, which are the drugs of choice in these infections. Thus, glycopeptide resistance represents a threat to the future of antimicrobial therapy in humans and animals. This study aimed to evaluate the profile of vancomycin resistance pheno-genotypically in Staphylococcus spp. milk samples from cows with mastitis. All 150 isolates (50 isolates of Staphylococcus aureus, 50 isolates of Staphylococcus intermedius and 50 isolates of Coagulase-negative Staphylococcus spp. (CNS) were evaluated using the tests of disk diffusion, agar microdilution and detection of vanA e vanB genes. The antimicrobial susceptibility test showed high resistance to beta-lactam antibiotics for all groups. In the disk diffusion test, 24% of coagulase-positive isolates showed resistance to teicoplanin (24/100) and 23% to vancomycin (23/100). In the agar microdilution test for detection of CIM, 16% of the isolates were resistant to vancomycin (16/100). Among the resistant isolates in the agar microdilution testing, 56.25% of the isolates (9 / 16) had MIC values ranging from 4-8μg/ml. Staphylococcus coagulase-negative showed no resistance to the evaluated glycopeptides. Genes for resistance to vancomycin were detected in 37.5% of vancomycin-resistant isolates (6 / 16). To seek a correlation between the presence of glycopeptide resistance and the ability of producing biofilm, tests were performed to characterize growth in Congo red agar and also detection of the biofilm adherence test in microplates. It were detected 66.66% and 90, 00%, respectively, of isolates producing biofilm. The production of "slime" presented no statistical correlation with resistance to glycopeptides. The sensitivity profile of Staphylococcus spp. was assessed against the bacteriocins produced by Lactobacillus paracasei, Lactobacillus acidophilus and Lactobacillus fermentum. Lactobacillus paracasei bacteriocins presented the highest inhibition capacity.application/pdfporUniversidade Federal Rural do Rio de JaneiroPrograma de Pós-Graduação em Ciências VeterináriasUFRRJBrasilInstituto de VeterináriaStaphylococcus spp. Mastitis. Vancomycin resistance. 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dc.title.por.fl_str_mv Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro
title Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro
spellingShingle Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro
Pribul, Bruno Rocha
Staphylococcus spp. Mastitis. Vancomycin resistance. Bacteriocins.
Staphylococcus spp. Mastite. Resistência a vancomicina. Bacteriocinas
Staphylococcus spp. Mastitis. Vancomycin resistance. Bacteriocins.
Medicina Veterinária
title_short Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro
title_full Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro
title_fullStr Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro
title_full_unstemmed Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro
title_sort Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro
author Pribul, Bruno Rocha
author_facet Pribul, Bruno Rocha
author_role author
dc.contributor.author.fl_str_mv Pribul, Bruno Rocha
dc.contributor.advisor1.fl_str_mv Souza, Miliane Moreira Soares de
dc.contributor.advisor1ID.fl_str_mv 1076198732
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0865211214618618
dc.contributor.authorID.fl_str_mv 5640401770
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4109229905380811
contributor_str_mv Souza, Miliane Moreira Soares de
dc.subject.eng.fl_str_mv Staphylococcus spp. Mastitis. Vancomycin resistance. Bacteriocins.
topic Staphylococcus spp. Mastitis. Vancomycin resistance. Bacteriocins.
Staphylococcus spp. Mastite. Resistência a vancomicina. Bacteriocinas
Staphylococcus spp. Mastitis. Vancomycin resistance. Bacteriocins.
Medicina Veterinária
dc.subject.por.fl_str_mv Staphylococcus spp. Mastite. Resistência a vancomicina. Bacteriocinas
Staphylococcus spp. Mastitis. Vancomycin resistance. Bacteriocins.
dc.subject.cnpq.fl_str_mv Medicina Veterinária
description PRIBUL, Bruno Rocha. Avaliação da Resistência a Vancomicina e do Perfil de Suscetibilidade a Bacteriocinas de Staphylococcus spp Isolados de Mastite Bovina da Região Sul do Estado do Rio de Janeiro. 41p Dissertação (Mestrado em Ciências Veterinárias). Instituto de Veterinária, Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2011. As bactérias do gênero Staphylococcus estão entre as mais implicadas na etiologia das mastites. Uma dificuldade adicional no controle das infecções bacterianas por este gênero é representada por sua freqüente resistência aos antimicrobianos. Os glicopeptídeos, como a vancomicina, aparecem como uma alternativa terapêutica devido ao crescente aumento de cepas estafilocócicas resistentes aos betalactâmicos, que são os fármacos de eleição nestas infecções. Desse modo, a resistência aos glicopeptídeos representa uma ameaça ao futuro da terapia antimicrobiana em humanos e animais. O presente trabalho buscou avaliar o perfil de resistência à vancomicina em isolados de Staphylococcus spp. oriundos de amostras de leite de vacas com mastite. Todos os 150 isolados estudados (50 Staphylococcus aureus, 50 Staphylococcus intermedius e 50 Staphylococcus spp. coagulase negativos), foram avaliados através dos testes de difusão em disco simples, microdiluição em ágar e detecção dos genes vanA e B. O teste de suscetibilidade antimicrobiana revelou elevada resistência aos beta-lactâmicos para todos os grupos avaliados. No ensaio de difusão em disco, os isolados coagulasepositivos apresentaram um perfil de resistência de 24% a teicoplanina (24/100) e 23% a vancomicina (23/100). No teste de microdiluição em ágar para detecção da CIM, 16% dos isolados apresentaram resistência à vancomicina (16/100). Entre os isolados resistentes no teste de microdiluição em ágar, 56,25% dos isolados (9/16) apresentaram valores de CIM que variaram entre 4-8μg/ml. Os Staphylococcus coagulase-negativos não apresentaram resistência aos glicopeptideos. Os genes de resistência à vancomicina foram detectados em 37,5% dos isolados vancomicina-resistentes (6/16). Para se buscar uma correlação entre a presença de resistência aos glicopeptideos e a capacidade de formação de biofilme foram realizados os testes de caracterização de crescimento em ágar vermelho congo e detecção de biofilme pelo teste de aderência em microplacas, onde foram detectados 66,66% e 90,00% respectivamente, de isolados produtores de biofilme. A produção de “slime” não apresentou correlação estatística com a resistência aos glicopeptídeos. O perfil de sensibilidade dos isolados de Staphylococcus spp. foi avaliado frente as bacteriocinas produzidas pelos Lactobacillus paracasei, Lactobacillus acidophillus e Lactobacillus fermentum. As bacteriocinas que apresentaram maior capacidade de inibição frente aos Saphylococcus spp. testados foram as produzidas pelos Lactobacillus paracasei
publishDate 2011
dc.date.issued.fl_str_mv 2011-02-24
dc.date.accessioned.fl_str_mv 2023-12-22T01:58:26Z
dc.date.available.fl_str_mv 2023-12-22T01:58:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PRIBUL, Bruno Rocha. Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro. 2011. 64 f. Dissertação (Programa de Pós-Graduação em Ciências Veterinárias - Universidade Federal Rural do Rio de Janeiro, Seropédica.
dc.identifier.uri.fl_str_mv https://rima.ufrrj.br/jspui/handle/20.500.14407/11901
identifier_str_mv PRIBUL, Bruno Rocha. Correlação entre o perfil de resistência antimicrobiana e a bacteriocinas em Staphylococcus SPP isolados de casos de mastite bovina ocorridos no Estado do Rio de Janeiro. 2011. 64 f. Dissertação (Programa de Pós-Graduação em Ciências Veterinárias - Universidade Federal Rural do Rio de Janeiro, Seropédica.
url https://rima.ufrrj.br/jspui/handle/20.500.14407/11901
dc.language.iso.fl_str_mv por
language por
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