Estudo fitoquímico e atividades biológicas de Jatropha curcas L.

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Sandra Santos
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/6059
Resumo: In this work was determined, for the first time, the volatile composition from fresh and dried leaves of six accessions (named PM-2, PM-7, PM-10, PM-11, PM-12 e PM-14) and a cultivar (EPAMIG, named here EMB) of Jatropha curcas L. The volatile compounds was extracted by hydrodistillation and then analyzed by GCMS. Thus, it was possible to observe that the (Z)-3-hexen-1-ol was the major compound in the fresh leaves (>70% in all accessions). Alcohols also predominated in the dried leaves, mainly (Z)-2-penten-1-ol and (Z)-3-hexen-1-ol, but others constituents were identified in significant proportions included benzenoid compounds such as benzaldehyde. The influence of drying on volatile composition from leaves of J. curcas was evaluated on the PM-2 accession. Besides, this work also describes the development of a High Performance Liquid Chromatograpy (HPLC) method for differentiation of six accessions and a cultivar (EPAMIG) from fresh and dried leaves of J. curcas. The methanol extracts of the leaves were analyzed by HPLC-DAD and after stages of optimizations, we obtained the fingerprint chromatograms. For a comparative analysis of the accessions and a cultivar, were applied chemometric tools of Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA), which it was possible to discriminate the samples from dried leaves according to their origins. The methanol extracts of the leaves were submitted to cytotoxicity assay against four tumor cell lines (HCT-8, HL-60, SF-295 and MDA-MB435), presenting IC50 values of 25.13 μg/ml, against HL-60 cell line for EMB sample considered promising by National Institute of Cancer, USA (NCI). The biological activity of this extracts was evaluated on the development of Spodoptera frugiperda, considered as a primary pest in corn crop. It was observed 60% larval mortality.
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spelling Ribeiro, Sandra Santoshttp://lattes.cnpq.br/3841279926110418Moraes, Valéria Regina de Souzahttp://lattes.cnpq.br/97155838017003412017-09-27T13:57:26Z2017-09-27T13:57:26Z2010-04-29https://ri.ufs.br/handle/riufs/6059In this work was determined, for the first time, the volatile composition from fresh and dried leaves of six accessions (named PM-2, PM-7, PM-10, PM-11, PM-12 e PM-14) and a cultivar (EPAMIG, named here EMB) of Jatropha curcas L. The volatile compounds was extracted by hydrodistillation and then analyzed by GCMS. Thus, it was possible to observe that the (Z)-3-hexen-1-ol was the major compound in the fresh leaves (>70% in all accessions). Alcohols also predominated in the dried leaves, mainly (Z)-2-penten-1-ol and (Z)-3-hexen-1-ol, but others constituents were identified in significant proportions included benzenoid compounds such as benzaldehyde. The influence of drying on volatile composition from leaves of J. curcas was evaluated on the PM-2 accession. Besides, this work also describes the development of a High Performance Liquid Chromatograpy (HPLC) method for differentiation of six accessions and a cultivar (EPAMIG) from fresh and dried leaves of J. curcas. The methanol extracts of the leaves were analyzed by HPLC-DAD and after stages of optimizations, we obtained the fingerprint chromatograms. For a comparative analysis of the accessions and a cultivar, were applied chemometric tools of Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA), which it was possible to discriminate the samples from dried leaves according to their origins. The methanol extracts of the leaves were submitted to cytotoxicity assay against four tumor cell lines (HCT-8, HL-60, SF-295 and MDA-MB435), presenting IC50 values of 25.13 μg/ml, against HL-60 cell line for EMB sample considered promising by National Institute of Cancer, USA (NCI). The biological activity of this extracts was evaluated on the development of Spodoptera frugiperda, considered as a primary pest in corn crop. It was observed 60% larval mortality.No presente trabalho foi determinado, pela primeira vez, os compostos voláteis das folhas frescas e secas de Jatropha curcas L. pela técnica de hidrodestilação, para seis diferentes acessos (denominados de PM-2, PM-7, PM-10, PM-11, PM- 12 e PM-14) e uma cultivar (EPAMIG, denominada de EMB). As análises foram realizadas através de CG/EM, obtendo-se o (Z)-3-hexen-1-ol (75,7±18,2%) como composto majoritário nas folhas frescas, com percentuais superiores a 70% entre os acessos. Nas folhas secas obteve-se como compostos majoritários o (Z)-2- penten-1-ol (14,9±11,3%), (Z)-3-hexen-1-ol (9,6±9,1%) e o benzaldeído (3,9±2,7%). Um experimento de secagem foi realizado para o acesso PM-2 com intuito de verificar o efeito da secagem na composição dos voláteis das folhas de J. curcas. Neste trabalho também foi desenvolvido um método por Cromatografia Líquida de Alta Eficiência (CLAE) para diferenciação dos seis acessos e da cultivar (EPAMIG) para folhas frescas e secas de J. curcas. Os extratos metanólicos das folhas foram analisados por CLAE-DAD e após etapas de otimizações, foram obtidos os cromatogramas fingerprints. Para uma análise comparativa entre os acessos e a cultivar, aplicaram-se ferramentas quimiométricas de Análise dos Componentes Principais (PCA) e Análise de Agrupamentos Hierárquicos (HCA), pelas quais foi possível diferenciar as amostras das folhas secas de acordo com suas procedências. Os extratos metanólicos também foram submetidos a ensaios de citotoxicidade in vitro para 04 linhagens de células tumorais humanas (HCT-8, HL-60, SF-295 e MDA-MB- 435), obtendo-se CI50 25,13 μg/mL, na linhagem HL-60 para a amostra da cultivar (EPAMIG). Este resultado está dentro do intervalo proposto pelo National Institute of Cancer, USA (NCI) para considerar extratos como promissores. A atividade biológica destes extratos também foi avaliada sobre o desenvolvimento de Spodoptera frugiperda, principal praga do milho, apresentando 60% de mortalidade larval.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de SergipePós-Graduação em QuímicaUFSBRJatropha curcas L.Compostos voláteisCLAEAnálises quimiométricasSpodoptera frugiperdaCitotoxicidade in vitroJatropha curcas L.Volatile compoundsHPLCChemometric analysisSpodoptera frugiperdaCytotoxicityCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAEstudo fitoquímico e atividades biológicas de Jatropha curcas L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSORIGINALSANDRA_SANTOS_RIBEIRO.pdfapplication/pdf2885002https://ri.ufs.br/jspui/bitstream/riufs/6059/1/SANDRA_SANTOS_RIBEIRO.pdf714dc9b161e93726b0b7cdf42b799124MD51TEXTSANDRA_SANTOS_RIBEIRO.pdf.txtSANDRA_SANTOS_RIBEIRO.pdf.txtExtracted texttext/plain222887https://ri.ufs.br/jspui/bitstream/riufs/6059/2/SANDRA_SANTOS_RIBEIRO.pdf.txtea71c7d437df1c48edf21087dc4741baMD52THUMBNAILSANDRA_SANTOS_RIBEIRO.pdf.jpgSANDRA_SANTOS_RIBEIRO.pdf.jpgGenerated Thumbnailimage/jpeg1219https://ri.ufs.br/jspui/bitstream/riufs/6059/3/SANDRA_SANTOS_RIBEIRO.pdf.jpg07dcf63aa43f03fd47d2dba6c65f7b86MD53riufs/60592018-01-17 21:28:57.651oai:ufs.br:riufs/6059Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-01-18T00:28:57Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.por.fl_str_mv Estudo fitoquímico e atividades biológicas de Jatropha curcas L.
title Estudo fitoquímico e atividades biológicas de Jatropha curcas L.
spellingShingle Estudo fitoquímico e atividades biológicas de Jatropha curcas L.
Ribeiro, Sandra Santos
Jatropha curcas L.
Compostos voláteis
CLAE
Análises quimiométricas
Spodoptera frugiperda
Citotoxicidade in vitro
Jatropha curcas L.
Volatile compounds
HPLC
Chemometric analysis
Spodoptera frugiperda
Cytotoxicity
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Estudo fitoquímico e atividades biológicas de Jatropha curcas L.
title_full Estudo fitoquímico e atividades biológicas de Jatropha curcas L.
title_fullStr Estudo fitoquímico e atividades biológicas de Jatropha curcas L.
title_full_unstemmed Estudo fitoquímico e atividades biológicas de Jatropha curcas L.
title_sort Estudo fitoquímico e atividades biológicas de Jatropha curcas L.
author Ribeiro, Sandra Santos
author_facet Ribeiro, Sandra Santos
author_role author
dc.contributor.author.fl_str_mv Ribeiro, Sandra Santos
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3841279926110418
dc.contributor.advisor1.fl_str_mv Moraes, Valéria Regina de Souza
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9715583801700341
contributor_str_mv Moraes, Valéria Regina de Souza
dc.subject.por.fl_str_mv Jatropha curcas L.
Compostos voláteis
CLAE
Análises quimiométricas
Spodoptera frugiperda
Citotoxicidade in vitro
topic Jatropha curcas L.
Compostos voláteis
CLAE
Análises quimiométricas
Spodoptera frugiperda
Citotoxicidade in vitro
Jatropha curcas L.
Volatile compounds
HPLC
Chemometric analysis
Spodoptera frugiperda
Cytotoxicity
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Jatropha curcas L.
Volatile compounds
HPLC
Chemometric analysis
Spodoptera frugiperda
Cytotoxicity
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
description In this work was determined, for the first time, the volatile composition from fresh and dried leaves of six accessions (named PM-2, PM-7, PM-10, PM-11, PM-12 e PM-14) and a cultivar (EPAMIG, named here EMB) of Jatropha curcas L. The volatile compounds was extracted by hydrodistillation and then analyzed by GCMS. Thus, it was possible to observe that the (Z)-3-hexen-1-ol was the major compound in the fresh leaves (>70% in all accessions). Alcohols also predominated in the dried leaves, mainly (Z)-2-penten-1-ol and (Z)-3-hexen-1-ol, but others constituents were identified in significant proportions included benzenoid compounds such as benzaldehyde. The influence of drying on volatile composition from leaves of J. curcas was evaluated on the PM-2 accession. Besides, this work also describes the development of a High Performance Liquid Chromatograpy (HPLC) method for differentiation of six accessions and a cultivar (EPAMIG) from fresh and dried leaves of J. curcas. The methanol extracts of the leaves were analyzed by HPLC-DAD and after stages of optimizations, we obtained the fingerprint chromatograms. For a comparative analysis of the accessions and a cultivar, were applied chemometric tools of Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA), which it was possible to discriminate the samples from dried leaves according to their origins. The methanol extracts of the leaves were submitted to cytotoxicity assay against four tumor cell lines (HCT-8, HL-60, SF-295 and MDA-MB435), presenting IC50 values of 25.13 μg/ml, against HL-60 cell line for EMB sample considered promising by National Institute of Cancer, USA (NCI). The biological activity of this extracts was evaluated on the development of Spodoptera frugiperda, considered as a primary pest in corn crop. It was observed 60% larval mortality.
publishDate 2010
dc.date.issued.fl_str_mv 2010-04-29
dc.date.accessioned.fl_str_mv 2017-09-27T13:57:26Z
dc.date.available.fl_str_mv 2017-09-27T13:57:26Z
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dc.publisher.program.fl_str_mv Pós-Graduação em Química
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dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal de Sergipe
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