Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Tipo de documento: | Trabalho de conclusão de curso |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da UFS |
| Texto Completo: | https://ri.ufs.br/jspui/handle/riufs/14929 |
Resumo: | Localized Cutaneous Leishmaniasis is one of the most common clinical forms of Leishmaniasis, characterized by the presence of ulcer-crusted lesions well localized on the body surface. In Brazil, the main species associated with this form is Leishmania braziliensis. For its treatment, the Pentavalent Antimonials, which requires parenteral use and are associated with important systemic toxicity, are used as the first line drugs. In addition, in recent years there has been an increasing in parasites resistant to this class of drugs. Thus, there is a global stimulus for the development of a new form of localized treatment that has similar efficacy to the traditional one, but with fewer associated side effects. In this context, the Liposome-encapsulated Usnic Acid Gelatine Membrane System presents as a viable treatment proposal, due to the anti-inflammatory, anti-oxidant, healing and leishmanicidal abilities due to this composition combination. Because of what has been exposed, the present work aimed to evaluate the in vitro potential of this system as a new form of treatment for Localized Cutaneous Leishmaniasis caused by L. braziliensis isolates. For this, the parasite viability was evaluated by direct counting after the exposure of the parasites with the proposed treatment. A significant reduction in the parasite viability of those treated with the present system was observed according to a dose-dependent effect of the Usnic Acid concentration. In addition, it was found that this form of treatment showed an irreversible character, in which the cultures treated with this system, even after the suspension of the treatment, presented less parasitic viability. Based on the results of the in vitro experiments, it is possible to affirm that the Liposome-encapsulated Usnic Acid Gelatine Membrane System presents potential to serve as a therapeutic option for Localized Cutaneous Leishmaniasis caused by parasites of the species L. braziliensis |
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Nunes, Tiago da SilvaTanajura, Diego Moura2022-01-07T18:36:29Z2022-01-07T18:36:29Z2019-09-23NUNES, Diego da Silva. Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo. 2019. 72 f. Monografia (Graduação em Medicina) - Departamento de Medicina, Universidade Federal de Sergipe, Aracaju, 2019.https://ri.ufs.br/jspui/handle/riufs/14929Localized Cutaneous Leishmaniasis is one of the most common clinical forms of Leishmaniasis, characterized by the presence of ulcer-crusted lesions well localized on the body surface. In Brazil, the main species associated with this form is Leishmania braziliensis. For its treatment, the Pentavalent Antimonials, which requires parenteral use and are associated with important systemic toxicity, are used as the first line drugs. In addition, in recent years there has been an increasing in parasites resistant to this class of drugs. Thus, there is a global stimulus for the development of a new form of localized treatment that has similar efficacy to the traditional one, but with fewer associated side effects. In this context, the Liposome-encapsulated Usnic Acid Gelatine Membrane System presents as a viable treatment proposal, due to the anti-inflammatory, anti-oxidant, healing and leishmanicidal abilities due to this composition combination. Because of what has been exposed, the present work aimed to evaluate the in vitro potential of this system as a new form of treatment for Localized Cutaneous Leishmaniasis caused by L. braziliensis isolates. For this, the parasite viability was evaluated by direct counting after the exposure of the parasites with the proposed treatment. A significant reduction in the parasite viability of those treated with the present system was observed according to a dose-dependent effect of the Usnic Acid concentration. In addition, it was found that this form of treatment showed an irreversible character, in which the cultures treated with this system, even after the suspension of the treatment, presented less parasitic viability. Based on the results of the in vitro experiments, it is possible to affirm that the Liposome-encapsulated Usnic Acid Gelatine Membrane System presents potential to serve as a therapeutic option for Localized Cutaneous Leishmaniasis caused by parasites of the species L. braziliensisA Leishmaniose Cutânea Localizada é uma das formas clínicas mais comuns de Leishmaniose, sendo caracterizada pela presença de lesões ulcero-crostosas bem localizadas e delimitadas na superfície corpórea. No Brasil, a principal espécie associada a esta forma é a Leishmania braziliensis. Para seu tratamento, utilizam-se como primeira linha os Antimoniais Pentavalentes, que são de uso parenteral e associados a importante toxicidade sistêmica. Além disso, destaca-se nos últimos anos, um aumento cada vez maior de parasitos resistentes a esta classe de drogas. Dessa forma, tem-se o estímulo para o desenvolvimento de uma nova forma de tratamento localizada que possua eficácia semelhante ao tradicional, mas com menos efeitos colaterais associados. Neste contexto, o Sistema de Membranas de Gelatina contendo Ácido Úsnico encapsulado em lipossomo apresenta-se como uma proposta viável de tratamento, em decorrência das capacidades anti-inflamatória, antioxidante, cicatrizante e leishmanicida associadas a essa combinação de composto. Diante do exposto, o presente trabalho teve como objetivo avaliar o potencial in vitro deste sistema como uma nova forma de tratamento para Leishmaniose Cutânea Localizada causadas por isolados de L. braziliensis. Para isso, foi avaliada a viabilidade parasitária através de contagem direta após a exposição dos parasitos com o tratamento proposto. Observou-se uma redução significativa da viabilidade parasitária daqueles tratados com o presente sistema de acordo com um efeito dose dependente da concentração do Ácido Úsnico. Ademais, constatou-se que esta forma de tratamento mostrou um caráter irreversível, em que as culturas tratadas com este sistema, mesmo após a suspensão do tratamento, apresentaram menor viabilidade parasitária. Frente os resultados dos experimentos in vitro realizados, é possível afirmar que o Sistema de Membranas de Gelatina contendo Ácido Úsnico encapsulado em lipossomo apresenta potencial de servir como uma opção terapêutica para Leishmaniose Cutânea Localizada causada por parasitos da espécie L. braziliensisAracajuporLeishmaniose CutâneaMembranas BioativasÁcido ÚsnicoCutaneous LeishmaniasisBioactive MembranesUsnic AcidAvaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal de SergipeDME - Departamento de Medicina – Aracaju - Presencialreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/14929/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALTiago_Silva_Nunes.pdfTiago_Silva_Nunes.pdfapplication/pdf1711607https://ri.ufs.br/jspui/bitstream/riufs/14929/2/Tiago_Silva_Nunes.pdf60aba58c03ebceae737a91e00cdc62f8MD52TEXTTiago_Silva_Nunes.pdf.txtTiago_Silva_Nunes.pdf.txtExtracted texttext/plain128255https://ri.ufs.br/jspui/bitstream/riufs/14929/3/Tiago_Silva_Nunes.pdf.txt0ce20c294cbbdf7841307948a0ebd743MD53THUMBNAILTiago_Silva_Nunes.pdf.jpgTiago_Silva_Nunes.pdf.jpgGenerated Thumbnailimage/jpeg1260https://ri.ufs.br/jspui/bitstream/riufs/14929/4/Tiago_Silva_Nunes.pdf.jpgcc1afccd6778494782958b5a1aca5f66MD54riufs/149292022-01-07 15:36:29.199oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2022-01-07T18:36:29Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
| dc.title.pt_BR.fl_str_mv |
Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo |
| title |
Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo |
| spellingShingle |
Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo Nunes, Tiago da Silva Leishmaniose Cutânea Membranas Bioativas Ácido Úsnico Cutaneous Leishmaniasis Bioactive Membranes Usnic Acid |
| title_short |
Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo |
| title_full |
Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo |
| title_fullStr |
Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo |
| title_full_unstemmed |
Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo |
| title_sort |
Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo |
| author |
Nunes, Tiago da Silva |
| author_facet |
Nunes, Tiago da Silva |
| author_role |
author |
| dc.contributor.author.fl_str_mv |
Nunes, Tiago da Silva |
| dc.contributor.advisor1.fl_str_mv |
Tanajura, Diego Moura |
| contributor_str_mv |
Tanajura, Diego Moura |
| dc.subject.por.fl_str_mv |
Leishmaniose Cutânea Membranas Bioativas Ácido Úsnico |
| topic |
Leishmaniose Cutânea Membranas Bioativas Ácido Úsnico Cutaneous Leishmaniasis Bioactive Membranes Usnic Acid |
| dc.subject.eng.fl_str_mv |
Cutaneous Leishmaniasis Bioactive Membranes Usnic Acid |
| description |
Localized Cutaneous Leishmaniasis is one of the most common clinical forms of Leishmaniasis, characterized by the presence of ulcer-crusted lesions well localized on the body surface. In Brazil, the main species associated with this form is Leishmania braziliensis. For its treatment, the Pentavalent Antimonials, which requires parenteral use and are associated with important systemic toxicity, are used as the first line drugs. In addition, in recent years there has been an increasing in parasites resistant to this class of drugs. Thus, there is a global stimulus for the development of a new form of localized treatment that has similar efficacy to the traditional one, but with fewer associated side effects. In this context, the Liposome-encapsulated Usnic Acid Gelatine Membrane System presents as a viable treatment proposal, due to the anti-inflammatory, anti-oxidant, healing and leishmanicidal abilities due to this composition combination. Because of what has been exposed, the present work aimed to evaluate the in vitro potential of this system as a new form of treatment for Localized Cutaneous Leishmaniasis caused by L. braziliensis isolates. For this, the parasite viability was evaluated by direct counting after the exposure of the parasites with the proposed treatment. A significant reduction in the parasite viability of those treated with the present system was observed according to a dose-dependent effect of the Usnic Acid concentration. In addition, it was found that this form of treatment showed an irreversible character, in which the cultures treated with this system, even after the suspension of the treatment, presented less parasitic viability. Based on the results of the in vitro experiments, it is possible to affirm that the Liposome-encapsulated Usnic Acid Gelatine Membrane System presents potential to serve as a therapeutic option for Localized Cutaneous Leishmaniasis caused by parasites of the species L. braziliensis |
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2019 |
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2019-09-23 |
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2022-01-07T18:36:29Z |
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2022-01-07T18:36:29Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/bachelorThesis |
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publishedVersion |
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NUNES, Diego da Silva. Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo. 2019. 72 f. Monografia (Graduação em Medicina) - Departamento de Medicina, Universidade Federal de Sergipe, Aracaju, 2019. |
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https://ri.ufs.br/jspui/handle/riufs/14929 |
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NUNES, Diego da Silva. Avaliação in vitro da atividade leishmanicida de membranas de gelatina contendo ácido úsnico encapsulado em lipossomo. 2019. 72 f. Monografia (Graduação em Medicina) - Departamento de Medicina, Universidade Federal de Sergipe, Aracaju, 2019. |
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por |
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por |
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Universidade Federal de Sergipe |
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DME - Departamento de Medicina – Aracaju - Presencial |
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reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS |
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