Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Daniel Ornelas
Data de Publicação: 2018
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: http://ri.ufs.br/jspui/handle/riufs/11651
Resumo: The species Jatropha curcas L. has a good yield of oil for biodiesel production. It has a resistance to water stress and it demonstrates a great potential as an insecticidal or medicinal agent. However, much research is still needed on the multiple uses of this species. Thus, whilst aiming to characterize 10 specific hybrids of J. curcas, the following assessments were carried out: i) a technological prospection regarding the bioactive properties of the Jatropha curcas L. species; ii) estimations of genetic diversity; iii) morphoagronomic characterizations of the fruits and seeds; and iv) the initial seed quality evaluations of the 10 hybrids of J. curcas that originated from diallel crosses. The survey was conducted in the databases of scientific, technological and patent research, on the websites of the Ministry of Health and the Ministry of Agriculture, Livestock and Supply, as well as on the National Agency of Sanitary Surveillance (ANVISA) website. The estimations of the genetic variabilities were performed by using Inter Simple Sequence Repeat (ISSR) molecular markers. Nine primers were used and six were selected with an 80.7% polymorphism. The morphoagronomic characterizations and the estimations of the phenotypic correlations between the variables were determined and deployed through track analyzes. The genetic, environmental, heritability and heteroses variances of the hybrids were estimated. In order to extract the oil, petroleum ether solvents were used and a Soxhlet type apparatus was also used. These obtained samples were derivatized and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Regarding the technological prospecting, there were academic records on the use of these extracts from different parts of J. curcas, evidencing the importance of this species for the formulation of phytosanitary products. However, there were only a few patent records regarding the use of these extracts as an insecticide. The average genetic similarity between the hybrids was 58.4%, with a greater genetic diversity observed between the 1x5 and 1x8 hybrids and with a greater similarity between the 3x13 and 1x5 hybrids. Morphoagronomically, the hybrids differed in their biometry, as well as in the oil contents of their seeds. A high heritability was observed for their agronomical characteristics, except for the mass of 100 seeds. The 1x5 hybrids stood out for their biometric characteristics. The highest oil content was extracted from the 3x13 hybrid seeds. The highest percentages that were found in the fatty acids were oleic acid (35.7% to 38.9%) and linoleic acid (30.6% to 37.8%). For their germination, the 1x5 (56%), 1x8 (55%), 1x13 (56%), 3x8 (61%), 4x5 (56%) and 4x8 (61%) hybrids stood out in the two evaluated years. As for their IVG, the highest values were observed in the 1x5 (3.34), 1x13 (3.70), 3x8 (3.34), 3x13 (3.05) and 4x8 (3.69) hybrids. The tetrazolium test verified that among the seeds that did not germinate, the hybrids 1x5, 3x5, 3x8 and 3x13 were not viable.
id UFS-2_115c4cd8f98cf814675292334b257552
oai_identifier_str oai:ufs.br:riufs/11651
network_acronym_str UFS-2
network_name_str Repositório Institucional da UFS
repository_id_str
spelling Ribeiro, Daniel OrnelasSilva-Mann, Renata2019-08-08T21:01:46Z2019-08-08T21:01:46Z2018-02-01RIBEIRO, Daniel Ornelas. Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos. 2018. 132 f. Tese (Doutorado em Agricultura e Biodiversidade) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019.http://ri.ufs.br/jspui/handle/riufs/11651The species Jatropha curcas L. has a good yield of oil for biodiesel production. It has a resistance to water stress and it demonstrates a great potential as an insecticidal or medicinal agent. However, much research is still needed on the multiple uses of this species. Thus, whilst aiming to characterize 10 specific hybrids of J. curcas, the following assessments were carried out: i) a technological prospection regarding the bioactive properties of the Jatropha curcas L. species; ii) estimations of genetic diversity; iii) morphoagronomic characterizations of the fruits and seeds; and iv) the initial seed quality evaluations of the 10 hybrids of J. curcas that originated from diallel crosses. The survey was conducted in the databases of scientific, technological and patent research, on the websites of the Ministry of Health and the Ministry of Agriculture, Livestock and Supply, as well as on the National Agency of Sanitary Surveillance (ANVISA) website. The estimations of the genetic variabilities were performed by using Inter Simple Sequence Repeat (ISSR) molecular markers. Nine primers were used and six were selected with an 80.7% polymorphism. The morphoagronomic characterizations and the estimations of the phenotypic correlations between the variables were determined and deployed through track analyzes. The genetic, environmental, heritability and heteroses variances of the hybrids were estimated. In order to extract the oil, petroleum ether solvents were used and a Soxhlet type apparatus was also used. These obtained samples were derivatized and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Regarding the technological prospecting, there were academic records on the use of these extracts from different parts of J. curcas, evidencing the importance of this species for the formulation of phytosanitary products. However, there were only a few patent records regarding the use of these extracts as an insecticide. The average genetic similarity between the hybrids was 58.4%, with a greater genetic diversity observed between the 1x5 and 1x8 hybrids and with a greater similarity between the 3x13 and 1x5 hybrids. Morphoagronomically, the hybrids differed in their biometry, as well as in the oil contents of their seeds. A high heritability was observed for their agronomical characteristics, except for the mass of 100 seeds. The 1x5 hybrids stood out for their biometric characteristics. The highest oil content was extracted from the 3x13 hybrid seeds. The highest percentages that were found in the fatty acids were oleic acid (35.7% to 38.9%) and linoleic acid (30.6% to 37.8%). For their germination, the 1x5 (56%), 1x8 (55%), 1x13 (56%), 3x8 (61%), 4x5 (56%) and 4x8 (61%) hybrids stood out in the two evaluated years. As for their IVG, the highest values were observed in the 1x5 (3.34), 1x13 (3.70), 3x8 (3.34), 3x13 (3.05) and 4x8 (3.69) hybrids. The tetrazolium test verified that among the seeds that did not germinate, the hybrids 1x5, 3x5, 3x8 and 3x13 were not viable.A espécie Jatropha curcas L. possui bom rendimento de óleo para produção de biodiesel, resistência ao estresse hídrico e grande potencial como agente inseticida e medicinal. Porém, ainda são necessárias pesquisas direcionadas aos múltiplos usos desta espécie. Assim, visando caracterizar 10 híbridos de J. curcas realizou-se: i) a prospecção tecnológica sobre as propriedades bioativas de J. curcas; ii) a estimativa da diversidade genética; iii) a caracterização morfoagronômica de frutos e sementes e iv) a avaliação da qualidade inicial de sementes dos 10 híbridos de J. curcas oriundos de cruzamentos dialélicos. A prospecção foi realizada em bancos de dados de pesquisa científica, tecnológica e de patentes, em sites do Ministério da Saúde, do Ministério da Agricultura, Pecuária e Abastecimento e da Agência Nacional de Vigilância Sanitária. A estimativa da variabilidade genética foi feita usando marcadores moleculares Inter Repetições de Sequências Simples (ISSR). Utilizou-se nove primers e seis foram selecionados com 80,7% de polimorfismo. A caracterização morfoagronômica e as estimativas das correlações fenotípicas entre as variáveis foram determinadas e desdobradas, por meio da análise de trilha. Estimou-se variâncias genéticas, ambientais, herdabilidade e heterose dos híbridos. Para a extração do óleo, usou-se o solvente éter de petróleo e empregouse aparelho tipo Soxhlet. Estas amostras foram derivatizadas e analisadas por cromatografia gasosa acoplada a espectrometria de massa. Quanto a prospecção tecnológica, há registros acadêmicos sobre o uso de extratos de diferentes partes de J. curcas, evidenciando a importância desta espécie para a formulação de produtos fitossanitários, porém, existem poucos registros de patentes referentes ao uso do extrato como inseticida. A similaridade genética média entre os híbridos foi de 58,4%, com maior diversidade genética observada entre os híbridos 1x5 e 1x8 e a maior similaridade entre os híbridos 3x13 e 1x5. Morfoagronomicamente, os híbridos diferem quanto a biometria e teor de óleo nas sementes. Observou-se alta herdabilidade para as características agronômicas, com exceção para massa de 100 sementes. O híbrido 1x5 destacase quanto à biometria de sementes. O maior teor de óleo foi obtido no híbrido 3x13. Os ácidos graxos encontrados em maior porcentagem foram o oleico (35,7% a 38,9%) e linoleico (30,6% a 37,8%), com destaque para o híbrido 4x5. Para germinação, nos dois anos avaliados, destacaram-se os híbridos 1x5 (56%), 1x8 (55%), 1x13 (56%), 3x8 (61%), 4x5 (56%) e 4x8 (61%). Quanto ao IVG, os maiores valores obtidos foram observados nos híbridos 1x5 (3,34), 1x13 (3,70), 3x8 (3,34), 3x13 (3,05) e 4x8 (3,69). Verificou-se pelo teste de tetrazólio que entre as sementes que não germinaram, as sementes não estavam viáveis para os híbridos 1x5, 3x5, 3x8 e 3x13.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSão Cristóvão, SEporPinhãoDiversidade biológicaSementesPinhão-mansoCaracterização morfoagronômicaDiversidade genéticaJatrophaMorphologySeedsGenetic diversityCIENCIAS AGRARIASVariabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicosGenetic variability and characterization of hybrids of Jatropha curcas L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPós-Graduação em Agricultura e BiodiversidadeUFSreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/11651/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALDANIEL_ORNELAS_RIBEIRO.pdfDANIEL_ORNELAS_RIBEIRO.pdfapplication/pdf1629938https://ri.ufs.br/jspui/bitstream/riufs/11651/2/DANIEL_ORNELAS_RIBEIRO.pdf5dc833db945e13aca6d05c22a4d881b1MD52TEXTDANIEL_ORNELAS_RIBEIRO.pdf.txtDANIEL_ORNELAS_RIBEIRO.pdf.txtExtracted texttext/plain393153https://ri.ufs.br/jspui/bitstream/riufs/11651/3/DANIEL_ORNELAS_RIBEIRO.pdf.txt9a32998191ca0cb1c245425a2c41fceaMD53THUMBNAILDANIEL_ORNELAS_RIBEIRO.pdf.jpgDANIEL_ORNELAS_RIBEIRO.pdf.jpgGenerated Thumbnailimage/jpeg1356https://ri.ufs.br/jspui/bitstream/riufs/11651/4/DANIEL_ORNELAS_RIBEIRO.pdf.jpg092f785051a4435650ab2a0b6f7df01fMD54riufs/116512019-08-08 18:01:47.132oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2019-08-08T21:01:47Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos
dc.title.alternative.por.fl_str_mv Genetic variability and characterization of hybrids of Jatropha curcas L.
title Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos
spellingShingle Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos
Ribeiro, Daniel Ornelas
Pinhão
Diversidade biológica
Sementes
Pinhão-manso
Caracterização morfoagronômica
Diversidade genética
Jatropha
Morphology
Seeds
Genetic diversity
CIENCIAS AGRARIAS
title_short Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos
title_full Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos
title_fullStr Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos
title_full_unstemmed Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos
title_sort Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos
author Ribeiro, Daniel Ornelas
author_facet Ribeiro, Daniel Ornelas
author_role author
dc.contributor.author.fl_str_mv Ribeiro, Daniel Ornelas
dc.contributor.advisor1.fl_str_mv Silva-Mann, Renata
contributor_str_mv Silva-Mann, Renata
dc.subject.por.fl_str_mv Pinhão
Diversidade biológica
Sementes
Pinhão-manso
Caracterização morfoagronômica
Diversidade genética
Jatropha
topic Pinhão
Diversidade biológica
Sementes
Pinhão-manso
Caracterização morfoagronômica
Diversidade genética
Jatropha
Morphology
Seeds
Genetic diversity
CIENCIAS AGRARIAS
dc.subject.eng.fl_str_mv Morphology
Seeds
Genetic diversity
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS
description The species Jatropha curcas L. has a good yield of oil for biodiesel production. It has a resistance to water stress and it demonstrates a great potential as an insecticidal or medicinal agent. However, much research is still needed on the multiple uses of this species. Thus, whilst aiming to characterize 10 specific hybrids of J. curcas, the following assessments were carried out: i) a technological prospection regarding the bioactive properties of the Jatropha curcas L. species; ii) estimations of genetic diversity; iii) morphoagronomic characterizations of the fruits and seeds; and iv) the initial seed quality evaluations of the 10 hybrids of J. curcas that originated from diallel crosses. The survey was conducted in the databases of scientific, technological and patent research, on the websites of the Ministry of Health and the Ministry of Agriculture, Livestock and Supply, as well as on the National Agency of Sanitary Surveillance (ANVISA) website. The estimations of the genetic variabilities were performed by using Inter Simple Sequence Repeat (ISSR) molecular markers. Nine primers were used and six were selected with an 80.7% polymorphism. The morphoagronomic characterizations and the estimations of the phenotypic correlations between the variables were determined and deployed through track analyzes. The genetic, environmental, heritability and heteroses variances of the hybrids were estimated. In order to extract the oil, petroleum ether solvents were used and a Soxhlet type apparatus was also used. These obtained samples were derivatized and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Regarding the technological prospecting, there were academic records on the use of these extracts from different parts of J. curcas, evidencing the importance of this species for the formulation of phytosanitary products. However, there were only a few patent records regarding the use of these extracts as an insecticide. The average genetic similarity between the hybrids was 58.4%, with a greater genetic diversity observed between the 1x5 and 1x8 hybrids and with a greater similarity between the 3x13 and 1x5 hybrids. Morphoagronomically, the hybrids differed in their biometry, as well as in the oil contents of their seeds. A high heritability was observed for their agronomical characteristics, except for the mass of 100 seeds. The 1x5 hybrids stood out for their biometric characteristics. The highest oil content was extracted from the 3x13 hybrid seeds. The highest percentages that were found in the fatty acids were oleic acid (35.7% to 38.9%) and linoleic acid (30.6% to 37.8%). For their germination, the 1x5 (56%), 1x8 (55%), 1x13 (56%), 3x8 (61%), 4x5 (56%) and 4x8 (61%) hybrids stood out in the two evaluated years. As for their IVG, the highest values were observed in the 1x5 (3.34), 1x13 (3.70), 3x8 (3.34), 3x13 (3.05) and 4x8 (3.69) hybrids. The tetrazolium test verified that among the seeds that did not germinate, the hybrids 1x5, 3x5, 3x8 and 3x13 were not viable.
publishDate 2018
dc.date.issued.fl_str_mv 2018-02-01
dc.date.accessioned.fl_str_mv 2019-08-08T21:01:46Z
dc.date.available.fl_str_mv 2019-08-08T21:01:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO, Daniel Ornelas. Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos. 2018. 132 f. Tese (Doutorado em Agricultura e Biodiversidade) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019.
dc.identifier.uri.fl_str_mv http://ri.ufs.br/jspui/handle/riufs/11651
identifier_str_mv RIBEIRO, Daniel Ornelas. Variabilidade genética e caracterização de híbridos de Jatropha curcas L. oriundos de cruzamentos dialélicos. 2018. 132 f. Tese (Doutorado em Agricultura e Biodiversidade) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019.
url http://ri.ufs.br/jspui/handle/riufs/11651
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.program.fl_str_mv Pós-Graduação em Agricultura e Biodiversidade
dc.publisher.initials.fl_str_mv UFS
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
instname:Universidade Federal de Sergipe (UFS)
instacron:UFS
instname_str Universidade Federal de Sergipe (UFS)
instacron_str UFS
institution UFS
reponame_str Repositório Institucional da UFS
collection Repositório Institucional da UFS
bitstream.url.fl_str_mv https://ri.ufs.br/jspui/bitstream/riufs/11651/1/license.txt
https://ri.ufs.br/jspui/bitstream/riufs/11651/2/DANIEL_ORNELAS_RIBEIRO.pdf
https://ri.ufs.br/jspui/bitstream/riufs/11651/3/DANIEL_ORNELAS_RIBEIRO.pdf.txt
https://ri.ufs.br/jspui/bitstream/riufs/11651/4/DANIEL_ORNELAS_RIBEIRO.pdf.jpg
bitstream.checksum.fl_str_mv 098cbbf65c2c15e1fb2e49c5d306a44c
5dc833db945e13aca6d05c22a4d881b1
9a32998191ca0cb1c245425a2c41fcea
092f785051a4435650ab2a0b6f7df01f
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)
repository.mail.fl_str_mv repositorio@academico.ufs.br
_version_ 1802110651819098112