Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | http://ri.ufs.br/jspui/handle/riufs/12116 |
Resumo: | Biotransformation of textile dyes resulting from reduction of azo dye binding or cytochrome P450 (CYP450) catalyzed oxidation has been studied due to the formation of byproducts with carcinogenic, mutagenic and genotoxic potential. Due to the difficulty of working with enzymes, the use of CYP450 model compounds has been highlighted. These catalysts have been used anchored to solid supports that prevent complex inactivation, increase catalytic activity and selectivity, and allow their reuse. Chitosan (QT) stands out among the other supports, because it is a biopolymer, a natural, renewable, biodegradable compound with affinity for metal ions. In this work, the Iron-Cyclam (FeCy) and Jacobsen's (JBN) catalysts were used as CYP450 models immobilized on chitosan and applied to the oxidation of reactive orange 16 (LD16), dispersed orange 3 (LD3) and methylene blue (AM) dyes. From the characterization of these supported materials, using Fourier Transform Infrared spectroscopy (FTIR) techniques, thermal analysis, and Scanning Electron Microscopy / Dispersive Energy Spectroscopy (SEM / EDS); Evidence could be obtained that catalysts are present in chitosan without significant changes in matrix structure. These materials were quantified using the FAAS technique, with values of 9.0x10-6 mol Jacobsen catalyst and 1.9x10-5 mol Fe-Cyclam per gram of chitosan. The dyes LR16 and LD3 were adsorbed by chitosan, making it impossible to study the action of catalysts. With the cationic dye, AM, it was possible to observe the oxidation of the dye and the influence of the pH in the reaction medium, presenting better results at pH 10 and 12. The highest percentage of oxidation was at pH 12 of 88% and 80% in the presence. the Jacobsen and Fe-Cyclam catalyst, respectively; revealing that the catalysts JBN-QT and FeCy-QT showed efficiency in AM oxidation. |
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Rocha, Evelyn Monique LimaBarros, Valéria Priscila de2019-10-18T23:08:37Z2019-10-18T23:08:37Z2019-07-29ROCHA, Evelyn Monique Lima. Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis. 2019. 58 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019.http://ri.ufs.br/jspui/handle/riufs/12116Biotransformation of textile dyes resulting from reduction of azo dye binding or cytochrome P450 (CYP450) catalyzed oxidation has been studied due to the formation of byproducts with carcinogenic, mutagenic and genotoxic potential. Due to the difficulty of working with enzymes, the use of CYP450 model compounds has been highlighted. These catalysts have been used anchored to solid supports that prevent complex inactivation, increase catalytic activity and selectivity, and allow their reuse. Chitosan (QT) stands out among the other supports, because it is a biopolymer, a natural, renewable, biodegradable compound with affinity for metal ions. In this work, the Iron-Cyclam (FeCy) and Jacobsen's (JBN) catalysts were used as CYP450 models immobilized on chitosan and applied to the oxidation of reactive orange 16 (LD16), dispersed orange 3 (LD3) and methylene blue (AM) dyes. From the characterization of these supported materials, using Fourier Transform Infrared spectroscopy (FTIR) techniques, thermal analysis, and Scanning Electron Microscopy / Dispersive Energy Spectroscopy (SEM / EDS); Evidence could be obtained that catalysts are present in chitosan without significant changes in matrix structure. These materials were quantified using the FAAS technique, with values of 9.0x10-6 mol Jacobsen catalyst and 1.9x10-5 mol Fe-Cyclam per gram of chitosan. The dyes LR16 and LD3 were adsorbed by chitosan, making it impossible to study the action of catalysts. With the cationic dye, AM, it was possible to observe the oxidation of the dye and the influence of the pH in the reaction medium, presenting better results at pH 10 and 12. The highest percentage of oxidation was at pH 12 of 88% and 80% in the presence. the Jacobsen and Fe-Cyclam catalyst, respectively; revealing that the catalysts JBN-QT and FeCy-QT showed efficiency in AM oxidation.A biotransformação de corantes têxteis que ocorre a partir da redução da ligação azo de corantes ou da oxidação catalisada pelo citocromo P450 (CYP450), tem sido estudada devido à formação de subprodutos com potencial carcinogênico, mutagênico e genotóxico. Devido à dificuldade de trabalhar com enzimas, vem se destacando o uso de compostos modelos do CYP450. Esses catalisadores têm sido utilizados ancorados a suportes sólidos que previnem a inativação dos complexos, aumentam a atividade catalítica e seletividade, além de permitir sua reutilização. A quitosana (QT) se destaca entre os demais suportes, pois trata-se de um biopolímero, um composto natural, renovável, biodegradável e com afinidade por íons metálicos. Neste trabalho foram utilizados os catalisadores Ferro-Cyclam (FeCy) e o catalisador de Jacobsen (JBN) como modelos do CYP450 imobilizados em quitosana e aplicados na oxidação dos corantes laranja reativo 16 (LD16), laranja disperso 3 (LD3) e azul de metileno (AM). A partir da caracterização desses materiais suportados, utilizando as técnicas de Espectroscopia no infravermelho por transformada de Fourier (FTIR), análises térmicas, e Microscopia Eletrônica de Varredura/Espectroscopia de Energia Dispersiva (MEV/EDS); pôde-se obter evidências de que os catalisadores estão presentes na quitosana sem alterações significativas da estrutura da matriz. Esses materiais foram quantificados utilizando a técnica de FAAS, apresentando valores de 9,0x10-6 mol de catalisador de Jacobsen e 1,9x10-5 mol de Fe-Cyclam, por grama de quitosana. Os corantes LR16 e LD3 foram adsorvidos pela quitosana, impossibilitando o estudo da ação dos catalisadores. Com o corante catiônico, AM, pôde-se observar a oxidação do corante e a influência do pH no meio reacional, apresentando melhores resultados em pH 10 e 12. A maior porcentagem de oxidação foi em pH 12 de 88% e 80% na presença do catalisador de Jacobsen e Fe-Cyclam, respectivamente; revelando que os catalisadores JBN-QT e FeCy-QT apresentaram eficiência na oxidação do AM.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSão Cristóvão, SEporQuímicaCatálise heterogêneaCorantesCitocromo P-450CatalisadoresCIENCIAS EXATAS E DA TERRA::QUIMICACatalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteisJacobsen's catalyst and iron cyclam supported in chitosan as cytochrome P450 models for textile dye oxidationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em QuímicaUFSreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTEXTEVELYN_MONIQUE_LIMA_ROCHA.pdf.txtEVELYN_MONIQUE_LIMA_ROCHA.pdf.txtExtracted texttext/plain98691https://ri.ufs.br/jspui/bitstream/riufs/12116/3/EVELYN_MONIQUE_LIMA_ROCHA.pdf.txt5e48679d6dcb0951e486c8304c91f18eMD53THUMBNAILEVELYN_MONIQUE_LIMA_ROCHA.pdf.jpgEVELYN_MONIQUE_LIMA_ROCHA.pdf.jpgGenerated Thumbnailimage/jpeg1428https://ri.ufs.br/jspui/bitstream/riufs/12116/4/EVELYN_MONIQUE_LIMA_ROCHA.pdf.jpg8ad9e898d9dcc8dbcd21864b900f2bdcMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/12116/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALEVELYN_MONIQUE_LIMA_ROCHA.pdfEVELYN_MONIQUE_LIMA_ROCHA.pdfapplication/pdf3531764https://ri.ufs.br/jspui/bitstream/riufs/12116/2/EVELYN_MONIQUE_LIMA_ROCHA.pdf5493a09ae37520ea7c5b0cec6fe8da18MD52riufs/121162019-10-18 20:08:38.15oai:ufs.br:riufs/12116TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvcihlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkZSBTZXJnaXBlIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyIHNldSB0cmFiYWxobyBubyBmb3JtYXRvIGVsZXRyw7RuaWNvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRlIFNlcmdpcGUgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIHNldSB0cmFiYWxobyBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZGUgU2VyZ2lwZSBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgZGUgc2V1IHRyYWJhbGhvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIHNldSB0cmFiYWxobyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyBuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0bywgcXVlIHNlamEgZGUgc2V1IGNvbmhlY2ltZW50bywgbsOjbyBpbmZyaW5nZSBkaXJlaXRvcyBhdXRvcmFpcyBkZSBuaW5ndcOpbS4KCkNhc28gbyB0cmFiYWxobyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiBkZWNsYXJhIHF1ZSBvYnRldmUgYSBwZXJtaXNzw6NvIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgw6AgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZGUgU2VyZ2lwZSBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zIGVzdMOhIGNsYXJhbWVudGUgaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGXDumRvLgoKQSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkZSBTZXJnaXBlIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUocykgb3UgbyhzKSBub21lKHMpIGRvKHMpIApkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRvIHRyYWJhbGhvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7Dp2EuIAo=Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2019-10-18T23:08:38Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis |
dc.title.alternative.eng.fl_str_mv |
Jacobsen's catalyst and iron cyclam supported in chitosan as cytochrome P450 models for textile dye oxidation |
title |
Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis |
spellingShingle |
Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis Rocha, Evelyn Monique Lima Química Catálise heterogênea Corantes Citocromo P-450 Catalisadores CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis |
title_full |
Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis |
title_fullStr |
Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis |
title_full_unstemmed |
Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis |
title_sort |
Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis |
author |
Rocha, Evelyn Monique Lima |
author_facet |
Rocha, Evelyn Monique Lima |
author_role |
author |
dc.contributor.author.fl_str_mv |
Rocha, Evelyn Monique Lima |
dc.contributor.advisor1.fl_str_mv |
Barros, Valéria Priscila de |
contributor_str_mv |
Barros, Valéria Priscila de |
dc.subject.por.fl_str_mv |
Química Catálise heterogênea Corantes Citocromo P-450 Catalisadores |
topic |
Química Catálise heterogênea Corantes Citocromo P-450 Catalisadores CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
Biotransformation of textile dyes resulting from reduction of azo dye binding or cytochrome P450 (CYP450) catalyzed oxidation has been studied due to the formation of byproducts with carcinogenic, mutagenic and genotoxic potential. Due to the difficulty of working with enzymes, the use of CYP450 model compounds has been highlighted. These catalysts have been used anchored to solid supports that prevent complex inactivation, increase catalytic activity and selectivity, and allow their reuse. Chitosan (QT) stands out among the other supports, because it is a biopolymer, a natural, renewable, biodegradable compound with affinity for metal ions. In this work, the Iron-Cyclam (FeCy) and Jacobsen's (JBN) catalysts were used as CYP450 models immobilized on chitosan and applied to the oxidation of reactive orange 16 (LD16), dispersed orange 3 (LD3) and methylene blue (AM) dyes. From the characterization of these supported materials, using Fourier Transform Infrared spectroscopy (FTIR) techniques, thermal analysis, and Scanning Electron Microscopy / Dispersive Energy Spectroscopy (SEM / EDS); Evidence could be obtained that catalysts are present in chitosan without significant changes in matrix structure. These materials were quantified using the FAAS technique, with values of 9.0x10-6 mol Jacobsen catalyst and 1.9x10-5 mol Fe-Cyclam per gram of chitosan. The dyes LR16 and LD3 were adsorbed by chitosan, making it impossible to study the action of catalysts. With the cationic dye, AM, it was possible to observe the oxidation of the dye and the influence of the pH in the reaction medium, presenting better results at pH 10 and 12. The highest percentage of oxidation was at pH 12 of 88% and 80% in the presence. the Jacobsen and Fe-Cyclam catalyst, respectively; revealing that the catalysts JBN-QT and FeCy-QT showed efficiency in AM oxidation. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-10-18T23:08:37Z |
dc.date.available.fl_str_mv |
2019-10-18T23:08:37Z |
dc.date.issued.fl_str_mv |
2019-07-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ROCHA, Evelyn Monique Lima. Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis. 2019. 58 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/12116 |
identifier_str_mv |
ROCHA, Evelyn Monique Lima. Catalisador de Jacobsen e ferro-cyclam suportados em quitosana como modelos do CYP450 para oxidação de corantes têxteis. 2019. 58 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019. |
url |
http://ri.ufs.br/jspui/handle/riufs/12116 |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Pós-Graduação em Química |
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UFS |
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