Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/jspui/handle/riufs/18285 |
Resumo: | Introduction: Naringenin (NAR) is an abundant flavonoid in citrus fruits and represents a promising compound for therapeutic purposes. It has been widely used in the face of its antioxidant, anti-inflammatory and anti-tumor activity. Objective: To evaluate the possible effects of naringenin incorporated into the membrane of gelatin containing liposome (GEL/NAR) on wound healing in a model of open cutaneous wounds in rodents. Material and Methods: Wistar male rats weighing 250-300 g were used to perform the experimental protocols. These were anesthetized intraperitoneally (Ketamine-80 mg / kg and Xylazine-10 mg / kg) and with an 8 mm metallic punch the excisional cutaneous wound was made in the dorsal region of the animal. After the procedure, the animals were treated for 7 days, single dose, with gelatine membrane containing liposome (BR), gelatine membrane containing liposome and 1% NAR (GEL/NAR 1%) and 2.5% (GEL/NAR 2.5%) or untreated (LP). The evaluation consisted of the measurement of the wound areas and the histological aspect regarding the tissue morphological characteristics after hematoxylin-eosin (HE) staining. The experimental protocols were approved by the Animal Research Ethics Committee (CEPA/UFS - 71/2015). Results: GEL/NAR 1% (56,16075 ± 3,653587; p<0,001) and GEL/NAR 2,5% (58,21967 ± 1,651126; p<0,001) reduced the area of the wound, when compared to the control group (37,41523 ± 6,276465). Regarding the intensity of the inflammatory response, LP, BR and NAR 1% were classified as moderate and GEL/NAR 2.5% as mild. According to the inflammatory phenotype, subacute inflammation was observed in the LP and BR groups due to the balance between neutrophils and lymphocytes and chronic nonspecific inflammation in the 1% and 2.5% NAR groups due to the predominance of lymphocytes. GEL / NAR 1% (80,000 ± 15,3188; p<0,01) and GEL / NAR 2.5% (75,2857 ± 17,3754; p<0,05)presented significant values regarding epithelization compared to LP (58,6571 ± 6,3892) and BR (51,5714 ± 11,0432),in addition demonstrated a greater vascularization and deposition of fibroblasts. Conclusion: In this sense, GEL/NAR provided improvement in the tissue repair evidenced by reduction of the wound area and regulation of the inflammatory reaction through the reduction of neutrophils and lymphocytes. Financial Support: CNPq, COPES/POSGRAP/UFS. |
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Gomes, Débora CostaBarreto, Rosana de Souza Siqueira2023-09-06T13:08:16Z2023-09-06T13:08:16Z2018GOMES, Débora Costa. Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual. 2018. 51f. Monografia (Graduação em Enfermagem) - Centro de Ciências Biológicas e da Saúde, Departamento de Enfermagem, Universidade Federal de Sergipe, Aracaju, 2018.https://ri.ufs.br/jspui/handle/riufs/18285Introduction: Naringenin (NAR) is an abundant flavonoid in citrus fruits and represents a promising compound for therapeutic purposes. It has been widely used in the face of its antioxidant, anti-inflammatory and anti-tumor activity. Objective: To evaluate the possible effects of naringenin incorporated into the membrane of gelatin containing liposome (GEL/NAR) on wound healing in a model of open cutaneous wounds in rodents. Material and Methods: Wistar male rats weighing 250-300 g were used to perform the experimental protocols. These were anesthetized intraperitoneally (Ketamine-80 mg / kg and Xylazine-10 mg / kg) and with an 8 mm metallic punch the excisional cutaneous wound was made in the dorsal region of the animal. After the procedure, the animals were treated for 7 days, single dose, with gelatine membrane containing liposome (BR), gelatine membrane containing liposome and 1% NAR (GEL/NAR 1%) and 2.5% (GEL/NAR 2.5%) or untreated (LP). The evaluation consisted of the measurement of the wound areas and the histological aspect regarding the tissue morphological characteristics after hematoxylin-eosin (HE) staining. The experimental protocols were approved by the Animal Research Ethics Committee (CEPA/UFS - 71/2015). Results: GEL/NAR 1% (56,16075 ± 3,653587; p<0,001) and GEL/NAR 2,5% (58,21967 ± 1,651126; p<0,001) reduced the area of the wound, when compared to the control group (37,41523 ± 6,276465). Regarding the intensity of the inflammatory response, LP, BR and NAR 1% were classified as moderate and GEL/NAR 2.5% as mild. According to the inflammatory phenotype, subacute inflammation was observed in the LP and BR groups due to the balance between neutrophils and lymphocytes and chronic nonspecific inflammation in the 1% and 2.5% NAR groups due to the predominance of lymphocytes. GEL / NAR 1% (80,000 ± 15,3188; p<0,01) and GEL / NAR 2.5% (75,2857 ± 17,3754; p<0,05)presented significant values regarding epithelization compared to LP (58,6571 ± 6,3892) and BR (51,5714 ± 11,0432),in addition demonstrated a greater vascularization and deposition of fibroblasts. Conclusion: In this sense, GEL/NAR provided improvement in the tissue repair evidenced by reduction of the wound area and regulation of the inflammatory reaction through the reduction of neutrophils and lymphocytes. Financial Support: CNPq, COPES/POSGRAP/UFS.Introdução: A Naringenina (NAR) é um flavonoide abundante em frutas cítricas e representa um composto promissor para fins terapêuticos. Tem sido muito utilizada diante da sua atividade antioxidante, anti-inflamatória e antitumoral. Objetivo: Avaliar os possíveis efeitos da naringenina incorporada a membrana de gelatina contendo lipossoma (GEL/NAR) sobre a cicatrização em um modelo de feridas cutâneas abertas em roedores. Material e Métodos: Para realização dos protocolos experimentais foram utilizados ratos machos Wistar, pesando entre 250 - 300g. Esses foram anestesiados por via intraperitoneal (Ketamina-80 mg/kg e Xilazina- 10 mg/kg) e com punch metálico de 8 mm realizou-se a confecção de ferida cutânea excisional na região dorsal do animal. Após o procedimento, os animais foram tratados por 7 dias, dose única, com membrana de gelatina contendo lipossoma (BR), membrana de gelatina contendo lipossoma e NAR a 1% (GEL/NAR 1%) e a 2,5% (GEL/NAR 2,5%) ou não tratados (LP). A avaliação consistiu na mensuração das áreas das feridas e no aspecto histológico quanto às características morfológicas teciduais após coloração em hematoxilina-eosina (HE). Os protocolos experimentais foram aprovados pelo Comitê de Ética em Pesquisa com Animal (CEPA/UFS - 71/2015). Resultados: GEL/NAR 1% (56,16075 ± 3,653587; p<0,001) e GEL/NAR 2,5% (58,21967 ± 1,651126; p<0,001) reduziram a área da ferida quando comparado ao grupo controle (37,41523 ± 6,276465). Quanto à intensidade da resposta inflamatória, LP, BR e NAR 1% foram classificadas como moderada e GEL/NAR 2,5% como leve. De acordo com o fenótipo inflamatório, observou-se inflamação subaguda nos grupos LP e BR em virtude do equilíbrio entre neutrófilos e linfócitos e inflamação crônica inespecífica nos grupos NAR 1% e 2,5% diante do predomínio de linfócitos. GEL/NAR 1% (80,000 ± 15,3188; p<0,01) e GEL/NAR 2,5% (75,2857 ± 17,3754; p<0,05) apresentou valores significativos quanto à epitelização em comparação com os grupos LP (58,6571 ± 6,3892) e BR (51,5714 ± 11,0432), além disso demonstraram uma maior vascularização e deposição de fibroblastos. Conclusão: Neste sentido, GEL/NAR proporcionou melhoria no reparo tecidual evidenciada pela redução da área da ferida e regulação da reação inflamatória através da redução de neutrófilos. Apoio Financeiro: CNPq, COPES/POSGRAP/UFS.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqAracajuporCicatrização de feridasFlavonoidesGelatinaLipossomasNaringeninaHealing woundFlavonoidGelatineLiposomesNaringeninCIENCIAS DA SAUDE::ENFERMAGEM::ENFERMAGEM MEDICO-CIRURGICAMembrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidualinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal de SergipeDEN - Departamento de Enfermagem – Aracaju - Presencialreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/18285/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALDebora_Gomes.pdfDebora_Gomes.pdfapplication/pdf1365767https://ri.ufs.br/jspui/bitstream/riufs/18285/2/Debora_Gomes.pdf37251750f8d60b05e25ed3ffd1362c02MD52TEXTDebora_Gomes.pdf.txtDebora_Gomes.pdf.txtExtracted texttext/plain83659https://ri.ufs.br/jspui/bitstream/riufs/18285/3/Debora_Gomes.pdf.txt03a5696bc802573bbd2910cd58219050MD53THUMBNAILDebora_Gomes.pdf.jpgDebora_Gomes.pdf.jpgGenerated Thumbnailimage/jpeg1195https://ri.ufs.br/jspui/bitstream/riufs/18285/4/Debora_Gomes.pdf.jpgd0c73dabb9f1aac563ae3ec1baf25763MD54riufs/182852023-09-06 10:08:21.535oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2023-09-06T13:08:21Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual |
title |
Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual |
spellingShingle |
Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual Gomes, Débora Costa Cicatrização de feridas Flavonoides Gelatina Lipossomas Naringenina Healing wound Flavonoid Gelatine Liposomes Naringenin CIENCIAS DA SAUDE::ENFERMAGEM::ENFERMAGEM MEDICO-CIRURGICA |
title_short |
Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual |
title_full |
Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual |
title_fullStr |
Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual |
title_full_unstemmed |
Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual |
title_sort |
Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual |
author |
Gomes, Débora Costa |
author_facet |
Gomes, Débora Costa |
author_role |
author |
dc.contributor.author.fl_str_mv |
Gomes, Débora Costa |
dc.contributor.advisor1.fl_str_mv |
Barreto, Rosana de Souza Siqueira |
contributor_str_mv |
Barreto, Rosana de Souza Siqueira |
dc.subject.por.fl_str_mv |
Cicatrização de feridas Flavonoides Gelatina Lipossomas Naringenina |
topic |
Cicatrização de feridas Flavonoides Gelatina Lipossomas Naringenina Healing wound Flavonoid Gelatine Liposomes Naringenin CIENCIAS DA SAUDE::ENFERMAGEM::ENFERMAGEM MEDICO-CIRURGICA |
dc.subject.eng.fl_str_mv |
Healing wound Flavonoid Gelatine Liposomes Naringenin |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::ENFERMAGEM::ENFERMAGEM MEDICO-CIRURGICA |
description |
Introduction: Naringenin (NAR) is an abundant flavonoid in citrus fruits and represents a promising compound for therapeutic purposes. It has been widely used in the face of its antioxidant, anti-inflammatory and anti-tumor activity. Objective: To evaluate the possible effects of naringenin incorporated into the membrane of gelatin containing liposome (GEL/NAR) on wound healing in a model of open cutaneous wounds in rodents. Material and Methods: Wistar male rats weighing 250-300 g were used to perform the experimental protocols. These were anesthetized intraperitoneally (Ketamine-80 mg / kg and Xylazine-10 mg / kg) and with an 8 mm metallic punch the excisional cutaneous wound was made in the dorsal region of the animal. After the procedure, the animals were treated for 7 days, single dose, with gelatine membrane containing liposome (BR), gelatine membrane containing liposome and 1% NAR (GEL/NAR 1%) and 2.5% (GEL/NAR 2.5%) or untreated (LP). The evaluation consisted of the measurement of the wound areas and the histological aspect regarding the tissue morphological characteristics after hematoxylin-eosin (HE) staining. The experimental protocols were approved by the Animal Research Ethics Committee (CEPA/UFS - 71/2015). Results: GEL/NAR 1% (56,16075 ± 3,653587; p<0,001) and GEL/NAR 2,5% (58,21967 ± 1,651126; p<0,001) reduced the area of the wound, when compared to the control group (37,41523 ± 6,276465). Regarding the intensity of the inflammatory response, LP, BR and NAR 1% were classified as moderate and GEL/NAR 2.5% as mild. According to the inflammatory phenotype, subacute inflammation was observed in the LP and BR groups due to the balance between neutrophils and lymphocytes and chronic nonspecific inflammation in the 1% and 2.5% NAR groups due to the predominance of lymphocytes. GEL / NAR 1% (80,000 ± 15,3188; p<0,01) and GEL / NAR 2.5% (75,2857 ± 17,3754; p<0,05)presented significant values regarding epithelization compared to LP (58,6571 ± 6,3892) and BR (51,5714 ± 11,0432),in addition demonstrated a greater vascularization and deposition of fibroblasts. Conclusion: In this sense, GEL/NAR provided improvement in the tissue repair evidenced by reduction of the wound area and regulation of the inflammatory reaction through the reduction of neutrophils and lymphocytes. Financial Support: CNPq, COPES/POSGRAP/UFS. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2023-09-06T13:08:16Z |
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2023-09-06T13:08:16Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/bachelorThesis |
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dc.identifier.citation.fl_str_mv |
GOMES, Débora Costa. Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual. 2018. 51f. Monografia (Graduação em Enfermagem) - Centro de Ciências Biológicas e da Saúde, Departamento de Enfermagem, Universidade Federal de Sergipe, Aracaju, 2018. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/jspui/handle/riufs/18285 |
identifier_str_mv |
GOMES, Débora Costa. Membrana bioativa de gelatina contendo naringenina em lipossomas no reparo tecidual. 2018. 51f. Monografia (Graduação em Enfermagem) - Centro de Ciências Biológicas e da Saúde, Departamento de Enfermagem, Universidade Federal de Sergipe, Aracaju, 2018. |
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https://ri.ufs.br/jspui/handle/riufs/18285 |
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Universidade Federal de Sergipe |
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DEN - Departamento de Enfermagem – Aracaju - Presencial |
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