Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis

Detalhes bibliográficos
Autor(a) principal: Costa, Maíra Pereira de Farias
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/3834
Resumo: This study investigated the occurrence of Enterococcus faecalis in clinical specimens obtained from 25 cases in which endodontic treatment failed and 10 cases of primary endodontic infection, both of which are associated with asymptomatic apical periodontitis. As well, in cases in which it was possible to recover this bacterium, the antibacterial actions of two medications (i) calcium hydroxide based paste (Calen), and (ii) the same mixed with 1% chlorexidine, were evaluated both in vivo and in vitro. Microbiological analysis was performed on the samples collected in the root canal in three phases of the root canal treatment, as follows: (1) before the chemonomechanical instrumentation; (2) after using the intracanal dressing; and (3) after 72 hours of having removed the intracanal dressing, at this period the root canals were empty. The samples were treated according to standard microbiological procedures. The results show that of the twenty-five cases of endodontic re-treatment, E. faecalis occurred in eighteen, and in ten cases of asymptomatic primary endodontic infection, E. faecalis occurred in only two. Also, the results show a higher percentage of occurrence of E. faecalis in teeth in which endodontic treatment failed (72%) compared with cases of primary endodontic infection associated with asymptomatic apical periodontitis (20%). Of the total cases contaminated with E. faecalis, nine were treated with calcium hydroxide based paste (Calen). Of these, six showed the occurrence of E. faecalis on the second sampling, and all the nine cases showed occurrence of this bacterium on the third sampling. Of the total cases contaminated with E. faecalis, eleven were treated with calcium hydroxide based paste (Calen) mixed with 1% chlorexidine. Of these, three showed occurrence of E. faecalis on the second sampling, and two showed occurrence of this bacterium on the third sampling. These results are not conclusive for in vivo experiments, but suggest that calcium hydroxide based paste mixed with 1% chlorexidine combats E. faecalis more effectively than calcium hydroxide alone; however, more certainty will require a larger sample size. For the direct exposure experiment (in vitro), samples of 21 teeth were analyzed. The results show effective antibacterial actions of the two medications to combat E. faecalis -Statistically similar at 95% confidence level.
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spelling Costa, Maíra Pereira de Fariashttp://lattes.cnpq.br/6990953237575666Trindade, Rita de Cássiahttp://lattes.cnpq.br/12936372316125632017-09-26T12:17:52Z2017-09-26T12:17:52Z2006-08-04COSTA, Maíra Pereira de Farias. Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis. 2006. 107 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Sergipe, Aracaju, 2006.https://ri.ufs.br/handle/riufs/3834This study investigated the occurrence of Enterococcus faecalis in clinical specimens obtained from 25 cases in which endodontic treatment failed and 10 cases of primary endodontic infection, both of which are associated with asymptomatic apical periodontitis. As well, in cases in which it was possible to recover this bacterium, the antibacterial actions of two medications (i) calcium hydroxide based paste (Calen), and (ii) the same mixed with 1% chlorexidine, were evaluated both in vivo and in vitro. Microbiological analysis was performed on the samples collected in the root canal in three phases of the root canal treatment, as follows: (1) before the chemonomechanical instrumentation; (2) after using the intracanal dressing; and (3) after 72 hours of having removed the intracanal dressing, at this period the root canals were empty. The samples were treated according to standard microbiological procedures. The results show that of the twenty-five cases of endodontic re-treatment, E. faecalis occurred in eighteen, and in ten cases of asymptomatic primary endodontic infection, E. faecalis occurred in only two. Also, the results show a higher percentage of occurrence of E. faecalis in teeth in which endodontic treatment failed (72%) compared with cases of primary endodontic infection associated with asymptomatic apical periodontitis (20%). Of the total cases contaminated with E. faecalis, nine were treated with calcium hydroxide based paste (Calen). Of these, six showed the occurrence of E. faecalis on the second sampling, and all the nine cases showed occurrence of this bacterium on the third sampling. Of the total cases contaminated with E. faecalis, eleven were treated with calcium hydroxide based paste (Calen) mixed with 1% chlorexidine. Of these, three showed occurrence of E. faecalis on the second sampling, and two showed occurrence of this bacterium on the third sampling. These results are not conclusive for in vivo experiments, but suggest that calcium hydroxide based paste mixed with 1% chlorexidine combats E. faecalis more effectively than calcium hydroxide alone; however, more certainty will require a larger sample size. For the direct exposure experiment (in vitro), samples of 21 teeth were analyzed. The results show effective antibacterial actions of the two medications to combat E. faecalis -Statistically similar at 95% confidence level.Este estudo investigou a presença de Enterococcus faecalis em espécimes clínicos obtidos de canais radiculares de 25 dentes cuja terapia endodôntica fracassou e 10 casos de necrose pulpar, ambos associados a lesões perirradiculares assintomáticas. Assim como, avaliou a ação antimicrobiana da pasta de hidróxido de cálcio (Calen) e dessa pasta associada à clorexidina a 1% , tanto in vivo, quanto in vitro nos casos em que foi possível resgatar a bactéria. Para análise microbiológica efetuou-se coleta dos canais radiculares em três etapas do tratamento endodôntico: antes do preparo químico-mecânico (primeira coleta), após a utilização da medicação intracanal (segunda coleta) e decorridas 72 horas da remoção da medicação (terceira coleta), período em que os canais permaneceram vazios. Dos 25 casos resistentes à terapia endodôntica, 18 apresentaram E. faecalis. Já nos 10 casos de necrose pulpar com lesão perirradicular, apenas em dois foi possível isolar o microrganismo. Os dados obtidos demonstraram que o percentual de ocorrência de E. faecalis foi mais elevado nos dentes que apresentavam tratamento resistente à terapia endodôntica do que nos casos de necrose pulpar associada à lesão perirradicular assintomática (72% X 20% respectivamente). Do total de casos contaminados por E.faecalis, nove foram medicados com pasta de hidróxido de cálcio (Calen) e 11 foram tratados com pasta de hidróxido de cálcio (Calen) associada à clorexidina a 1%. Dos nove casos medicados com hidróxido de cálcio, seis apresentaram E. faecalis após a medicação. Na terceira coleta, todos os nove casos apresentaram contaminação por E. faecalis. Dos 11 casos medicados com pasta de hidróxido de cálcio (Calen) associada à clorexidina a 1%, em três, isolou-se a bactéria na segunda coleta microbiológica. Na terceira coleta, apenas em dois casos foi possível resgatar E. faecalis. Os resultados para a investigação in vivo, quanto à eficácia das medicações, não são conclusivos, mas sugerem que a associação da clorexidina a 1% à pasta de hidróxido de cálcio apresenta uma melhor eficácia no combate ao E. faecalis. Para que seja feita qualquer inferência nessa direção, devemos considerar um maior n amostral. Amostras de E. faecalis de 21 dentes foram utilizadas no teste por exposição direta (in vitro). Os dois medicamentos apresentaram ação antimicrobiana sobre o E.faecalis, não havendo diferença estatística significante entre as pastas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de SergipePós-Graduação em Ciências da SaúdeUFSBRClorexidinaEnterococcus faecalisHidróxido de cálcioCanal radicularChlorexidineEnterococcus faecalisCalcium hydroxideRoot canalCNPQ::CIENCIAS DA SAUDE::MEDICINAEfetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSTEXTMAIRA_PEREIRA_FARIAS_COSTA.pdf.txtMAIRA_PEREIRA_FARIAS_COSTA.pdf.txtExtracted texttext/plain173449https://ri.ufs.br/jspui/bitstream/riufs/3834/2/MAIRA_PEREIRA_FARIAS_COSTA.pdf.txtc74f45e89b287f7529ff80bae77f4d87MD52THUMBNAILMAIRA_PEREIRA_FARIAS_COSTA.pdf.jpgMAIRA_PEREIRA_FARIAS_COSTA.pdf.jpgGenerated Thumbnailimage/jpeg1488https://ri.ufs.br/jspui/bitstream/riufs/3834/3/MAIRA_PEREIRA_FARIAS_COSTA.pdf.jpg44f9f02a5804253457c77e1c1bae8348MD53ORIGINALMAIRA_PEREIRA_FARIAS_COSTA.pdfapplication/pdf575997https://ri.ufs.br/jspui/bitstream/riufs/3834/1/MAIRA_PEREIRA_FARIAS_COSTA.pdfe955c0f8f456732d2f675f1b84445107MD51riufs/38342017-11-28 16:37:51.28oai:ufs.br:riufs/3834Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-28T19:37:51Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.por.fl_str_mv Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis
title Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis
spellingShingle Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis
Costa, Maíra Pereira de Farias
Clorexidina
Enterococcus faecalis
Hidróxido de cálcio
Canal radicular
Chlorexidine
Enterococcus faecalis
Calcium hydroxide
Root canal
CNPQ::CIENCIAS DA SAUDE::MEDICINA
title_short Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis
title_full Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis
title_fullStr Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis
title_full_unstemmed Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis
title_sort Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis
author Costa, Maíra Pereira de Farias
author_facet Costa, Maíra Pereira de Farias
author_role author
dc.contributor.author.fl_str_mv Costa, Maíra Pereira de Farias
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6990953237575666
dc.contributor.advisor1.fl_str_mv Trindade, Rita de Cássia
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1293637231612563
contributor_str_mv Trindade, Rita de Cássia
dc.subject.por.fl_str_mv Clorexidina
Enterococcus faecalis
Hidróxido de cálcio
Canal radicular
topic Clorexidina
Enterococcus faecalis
Hidróxido de cálcio
Canal radicular
Chlorexidine
Enterococcus faecalis
Calcium hydroxide
Root canal
CNPQ::CIENCIAS DA SAUDE::MEDICINA
dc.subject.eng.fl_str_mv Chlorexidine
Enterococcus faecalis
Calcium hydroxide
Root canal
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::MEDICINA
description This study investigated the occurrence of Enterococcus faecalis in clinical specimens obtained from 25 cases in which endodontic treatment failed and 10 cases of primary endodontic infection, both of which are associated with asymptomatic apical periodontitis. As well, in cases in which it was possible to recover this bacterium, the antibacterial actions of two medications (i) calcium hydroxide based paste (Calen), and (ii) the same mixed with 1% chlorexidine, were evaluated both in vivo and in vitro. Microbiological analysis was performed on the samples collected in the root canal in three phases of the root canal treatment, as follows: (1) before the chemonomechanical instrumentation; (2) after using the intracanal dressing; and (3) after 72 hours of having removed the intracanal dressing, at this period the root canals were empty. The samples were treated according to standard microbiological procedures. The results show that of the twenty-five cases of endodontic re-treatment, E. faecalis occurred in eighteen, and in ten cases of asymptomatic primary endodontic infection, E. faecalis occurred in only two. Also, the results show a higher percentage of occurrence of E. faecalis in teeth in which endodontic treatment failed (72%) compared with cases of primary endodontic infection associated with asymptomatic apical periodontitis (20%). Of the total cases contaminated with E. faecalis, nine were treated with calcium hydroxide based paste (Calen). Of these, six showed the occurrence of E. faecalis on the second sampling, and all the nine cases showed occurrence of this bacterium on the third sampling. Of the total cases contaminated with E. faecalis, eleven were treated with calcium hydroxide based paste (Calen) mixed with 1% chlorexidine. Of these, three showed occurrence of E. faecalis on the second sampling, and two showed occurrence of this bacterium on the third sampling. These results are not conclusive for in vivo experiments, but suggest that calcium hydroxide based paste mixed with 1% chlorexidine combats E. faecalis more effectively than calcium hydroxide alone; however, more certainty will require a larger sample size. For the direct exposure experiment (in vitro), samples of 21 teeth were analyzed. The results show effective antibacterial actions of the two medications to combat E. faecalis -Statistically similar at 95% confidence level.
publishDate 2006
dc.date.issued.fl_str_mv 2006-08-04
dc.date.accessioned.fl_str_mv 2017-09-26T12:17:52Z
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dc.identifier.citation.fl_str_mv COSTA, Maíra Pereira de Farias. Efetividade do gluconato de clorexidina a 1% associado à pasta de hidróxido de cálcio (Calen), como medicação intracanal, no combate ao Enterococcus faecalis. 2006. 107 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Sergipe, Aracaju, 2006.
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