Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | http://ri.ufs.br/jspui/handle/riufs/17526 |
Resumo: | Introduction: Parkinson's disease (PD) is a chronic and progressive neurodegenerative disease mainly related to the death of dopaminergic neurons. The treatment is carried out with drugs that promote the more significant activity of the dopaminergic pathway; however, PD has no cure. Non-pharmacological strategies have been studied to delay the onset of the disease, minimize symptoms, or both. Among these strategies, physical exercise stands out for promoting neuroprotection. Thus, the present study evaluated whether physical exercise acts as a neuroprotective factor in a progressive model of parkinsonism induced by reserpine (RES) in rats. Method: 48 male Wistar rats, aged 6-7 months old, weighing 350-450 g, were used with authorization from the Ethics Committee for Research with Animals of the Federal University of Sergipe (protocol number: 5483070122). Animals were allocated into six groups (n = 8 per group): 1) CTR-Sedentary (CTR-SED), 2) RES-Sedentary (RESSED), 3) CTR-Exercise (CTR-EXE), 4) RES-Exercise Prior (RES-EXE-PRE), 5) RESSynchronous Exercise (RES-EXE-SIN) and 6) RES-Delayed Exercise (RES-EXETAR). Animals received ten subcutaneous injections of vehicle (CTR groups) or RES 0.1 mg/kg (RES groups), one every 48h. Animals in groups 1 and 2 were not submitted to physical exercise sessions. Animals in groups 3 and 4 were submitted to physical exercise sessions 15 days before starting the vehicle or RES injections. The animals in group 5 started the physical exercise sessions concomitantly with the beginning of the injections with RES. The animals in group 6 only started the physical exercise sessions 48h after the sixth RES injection. Throughout the treatment, the animals were submitted to behavioral tests: (a) catalepsy test (every 48h), (b) oral movements (days 14 and 20), and open field test (days 8 and 20). Throughout the experiment, the body mass of the animals was also measured. On day 20, the rats were anesthetized and perfused; their brains were removed and subjected to immunohistochemistry for Tyrosine Hydroxylase (TH). Results: Prior physical exercise was able to delay the onset of motor changes induced by RES in the catalepsy test. Besides, the animals in all groups with exercise showed a lower disease progression. Prior exercise was also able to prevent anxiety-like behavior (open field - day 8); and partially prevent motor damage in the oral movement, in the open field test, and loss of body mass. Only animals with prior exercise did not present a reduction in immunoreactivity for tyrosine hydroxylase (TH), an enzyme involved in the dopamine synthesis pathway. Conclusion: our study suggests that physical exercise promotes a neuroprotective effect, slowing the progression of parkinsonism induced by RES in rats. |
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Silva, Marcelo Nunes daSantos, José Ronaldo dos2023-05-09T21:32:12Z2023-05-09T21:32:12Z2023-03-30SILVA, Marcelo Nunes da. Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos. 2023. 70 f. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de Sergipe, São Cristóvão, 2023.http://ri.ufs.br/jspui/handle/riufs/17526Introduction: Parkinson's disease (PD) is a chronic and progressive neurodegenerative disease mainly related to the death of dopaminergic neurons. The treatment is carried out with drugs that promote the more significant activity of the dopaminergic pathway; however, PD has no cure. Non-pharmacological strategies have been studied to delay the onset of the disease, minimize symptoms, or both. Among these strategies, physical exercise stands out for promoting neuroprotection. Thus, the present study evaluated whether physical exercise acts as a neuroprotective factor in a progressive model of parkinsonism induced by reserpine (RES) in rats. Method: 48 male Wistar rats, aged 6-7 months old, weighing 350-450 g, were used with authorization from the Ethics Committee for Research with Animals of the Federal University of Sergipe (protocol number: 5483070122). Animals were allocated into six groups (n = 8 per group): 1) CTR-Sedentary (CTR-SED), 2) RES-Sedentary (RESSED), 3) CTR-Exercise (CTR-EXE), 4) RES-Exercise Prior (RES-EXE-PRE), 5) RESSynchronous Exercise (RES-EXE-SIN) and 6) RES-Delayed Exercise (RES-EXETAR). Animals received ten subcutaneous injections of vehicle (CTR groups) or RES 0.1 mg/kg (RES groups), one every 48h. Animals in groups 1 and 2 were not submitted to physical exercise sessions. Animals in groups 3 and 4 were submitted to physical exercise sessions 15 days before starting the vehicle or RES injections. The animals in group 5 started the physical exercise sessions concomitantly with the beginning of the injections with RES. The animals in group 6 only started the physical exercise sessions 48h after the sixth RES injection. Throughout the treatment, the animals were submitted to behavioral tests: (a) catalepsy test (every 48h), (b) oral movements (days 14 and 20), and open field test (days 8 and 20). Throughout the experiment, the body mass of the animals was also measured. On day 20, the rats were anesthetized and perfused; their brains were removed and subjected to immunohistochemistry for Tyrosine Hydroxylase (TH). Results: Prior physical exercise was able to delay the onset of motor changes induced by RES in the catalepsy test. Besides, the animals in all groups with exercise showed a lower disease progression. Prior exercise was also able to prevent anxiety-like behavior (open field - day 8); and partially prevent motor damage in the oral movement, in the open field test, and loss of body mass. Only animals with prior exercise did not present a reduction in immunoreactivity for tyrosine hydroxylase (TH), an enzyme involved in the dopamine synthesis pathway. Conclusion: our study suggests that physical exercise promotes a neuroprotective effect, slowing the progression of parkinsonism induced by RES in rats.Introdução: A doença de Parkinson (DP) é uma doença neurodegenerativa, crônica e progressiva, relacionada principalmente à morte de neurônios dopaminérgicos. Seu tratamento é realizado com medicamentos que promovem uma maior atividade da via dopaminérgica; entretanto, a DP não tem cura. Estratégias não farmacológicas têm sido estudadas como forma de retardar o surgimento e/ou minimizar os sintomas da doença. Entre as estratégias, destaca-se o exercício físico, que é capaz de promover neuroproteção. Nesse sentido, o objetivo do presente estudo, foi avaliar se o exercício físico atua como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina (RES) em ratos. Método: Foram utilizados 48 ratos machos da linhagem Wistar, entre 6 e 7 meses de idade, pesando, em média 350-450 gramas, com autorização do Comitê de Ética em Pesquisa com Animais (CEPA) da UFS (número do protocolo: 5483070122). Os animais foram divididos em 6 grupos (n = 8 por grupo): 1- CTR-sedentário (CTR-SED), 2-RES-sedentário (RESSED), 3- CTR-Exercício (CTR+EXE), 4- RES-Exercício-Prévio (RES-EXE-PRE), 5- RES-Exercício síncrono (RES-EXE-SIN) e 6-RES-Exercício-Tardio (RES-EXE-TAR). Os animais receberam 10 injeções de veículo (grupos CTR) ou RES 0,1 mg/kg s.c. (grupos RES), uma a cada 48h. Os animais dos grupos 1 e 2 não foram submetidos a sessões de exercício físico. Os animais dos grupos 3 e 4 foram submetidos a sessões de exercício físico 15 dias antes do início das injeções de veículo ou RES. Os animais do grupo 5 iniciaram as sessões de exercício físico concomitante ao início das injeções com RES. Os animais do grupo 6 apenas iniciaram as sessões de exercício físico 48h após a sexta injeção de RES. Ao longo do tratamento, os animais foram submetidos a testes comportamentais: (a) catalepsia (a cada 48h), (b) movimentos orais (dias 14 e 20) e campo aberto (dia 8 e 20). Ao longo do experimento os animais também foram aferidos quanto a massa corporal. No dia 20, os ratos foram anestesiados, perfundidos, seus encéfalos foram removidos e submetidos a imunohistoquímica para Tirosina Hidroxilase (TH). Resultados: Observou-se que o exercício físico prévio foi capaz de retardar o início das alterações motoras, induzidas pela RES, no teste de catalepsia, além dos animais de todos os grupos com exercício apresentarem uma menor progressividade da doença. O exercício prévio também foi capaz de impedir parcialmente os prejuízos motores no teste de movimentos orais e no campo aberto, a perda de massa corporal e impediu o comportamento tipo ansioso (campo aberto - dia 8). Apenas os animais com exercício prévio não apresentaram redução da imunorreatividade para a tirosina hidroxilase (TH), enzima envolvida na via da síntese da dopamina. Conclusão: nosso estudo sugere que a prática de exercício físico promove um efeito neuroprotetor, desacelerando a progressividade do parkinsonismo induzido pela RES em ratos.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSão CristóvãoporDoença de ParkinsonAtividade físicaNeuroproteçãoDopaminaParkinson's diseasePhysical activityNeuroprotectionDopamineCIENCIAS BIOLOGICAS::FISIOLOGIAExercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratosPhysical exercise as a neuroprotective factor in a progressive model of reserpine-induced parkinsonism in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em Ciências FisiológicasUniversidade Federal de Sergipe (UFS)reponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/17526/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALMARCELO_NUNES_SILVA.pdfMARCELO_NUNES_SILVA.pdfapplication/pdf2491235https://ri.ufs.br/jspui/bitstream/riufs/17526/2/MARCELO_NUNES_SILVA.pdf69aa6ef6dcc9da506f05026b49c83f76MD52TEXTMARCELO_NUNES_SILVA.pdf.txtMARCELO_NUNES_SILVA.pdf.txtExtracted texttext/plain136537https://ri.ufs.br/jspui/bitstream/riufs/17526/3/MARCELO_NUNES_SILVA.pdf.txt75e02e1b8f0f00fbf04099782041bc7bMD53THUMBNAILMARCELO_NUNES_SILVA.pdf.jpgMARCELO_NUNES_SILVA.pdf.jpgGenerated Thumbnailimage/jpeg1397https://ri.ufs.br/jspui/bitstream/riufs/17526/4/MARCELO_NUNES_SILVA.pdf.jpg178c334927885c68d464ba8a303b1afeMD54riufs/175262023-05-09 18:32:12.583oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2023-05-09T21:32:12Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos |
dc.title.alternative.eng.fl_str_mv |
Physical exercise as a neuroprotective factor in a progressive model of reserpine-induced parkinsonism in rats |
title |
Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos |
spellingShingle |
Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos Silva, Marcelo Nunes da Doença de Parkinson Atividade física Neuroproteção Dopamina Parkinson's disease Physical activity Neuroprotection Dopamine CIENCIAS BIOLOGICAS::FISIOLOGIA |
title_short |
Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos |
title_full |
Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos |
title_fullStr |
Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos |
title_full_unstemmed |
Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos |
title_sort |
Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos |
author |
Silva, Marcelo Nunes da |
author_facet |
Silva, Marcelo Nunes da |
author_role |
author |
dc.contributor.author.fl_str_mv |
Silva, Marcelo Nunes da |
dc.contributor.advisor1.fl_str_mv |
Santos, José Ronaldo dos |
contributor_str_mv |
Santos, José Ronaldo dos |
dc.subject.por.fl_str_mv |
Doença de Parkinson Atividade física Neuroproteção Dopamina Parkinson's disease Physical activity Neuroprotection Dopamine |
topic |
Doença de Parkinson Atividade física Neuroproteção Dopamina Parkinson's disease Physical activity Neuroprotection Dopamine CIENCIAS BIOLOGICAS::FISIOLOGIA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FISIOLOGIA |
description |
Introduction: Parkinson's disease (PD) is a chronic and progressive neurodegenerative disease mainly related to the death of dopaminergic neurons. The treatment is carried out with drugs that promote the more significant activity of the dopaminergic pathway; however, PD has no cure. Non-pharmacological strategies have been studied to delay the onset of the disease, minimize symptoms, or both. Among these strategies, physical exercise stands out for promoting neuroprotection. Thus, the present study evaluated whether physical exercise acts as a neuroprotective factor in a progressive model of parkinsonism induced by reserpine (RES) in rats. Method: 48 male Wistar rats, aged 6-7 months old, weighing 350-450 g, were used with authorization from the Ethics Committee for Research with Animals of the Federal University of Sergipe (protocol number: 5483070122). Animals were allocated into six groups (n = 8 per group): 1) CTR-Sedentary (CTR-SED), 2) RES-Sedentary (RESSED), 3) CTR-Exercise (CTR-EXE), 4) RES-Exercise Prior (RES-EXE-PRE), 5) RESSynchronous Exercise (RES-EXE-SIN) and 6) RES-Delayed Exercise (RES-EXETAR). Animals received ten subcutaneous injections of vehicle (CTR groups) or RES 0.1 mg/kg (RES groups), one every 48h. Animals in groups 1 and 2 were not submitted to physical exercise sessions. Animals in groups 3 and 4 were submitted to physical exercise sessions 15 days before starting the vehicle or RES injections. The animals in group 5 started the physical exercise sessions concomitantly with the beginning of the injections with RES. The animals in group 6 only started the physical exercise sessions 48h after the sixth RES injection. Throughout the treatment, the animals were submitted to behavioral tests: (a) catalepsy test (every 48h), (b) oral movements (days 14 and 20), and open field test (days 8 and 20). Throughout the experiment, the body mass of the animals was also measured. On day 20, the rats were anesthetized and perfused; their brains were removed and subjected to immunohistochemistry for Tyrosine Hydroxylase (TH). Results: Prior physical exercise was able to delay the onset of motor changes induced by RES in the catalepsy test. Besides, the animals in all groups with exercise showed a lower disease progression. Prior exercise was also able to prevent anxiety-like behavior (open field - day 8); and partially prevent motor damage in the oral movement, in the open field test, and loss of body mass. Only animals with prior exercise did not present a reduction in immunoreactivity for tyrosine hydroxylase (TH), an enzyme involved in the dopamine synthesis pathway. Conclusion: our study suggests that physical exercise promotes a neuroprotective effect, slowing the progression of parkinsonism induced by RES in rats. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-05-09T21:32:12Z |
dc.date.available.fl_str_mv |
2023-05-09T21:32:12Z |
dc.date.issued.fl_str_mv |
2023-03-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Marcelo Nunes da. Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos. 2023. 70 f. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de Sergipe, São Cristóvão, 2023. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/17526 |
identifier_str_mv |
SILVA, Marcelo Nunes da. Exercício físico como fator neuroprotetor em um modelo progressivo de parkinsonismo induzido por reserpina em ratos. 2023. 70 f. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de Sergipe, São Cristóvão, 2023. |
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http://ri.ufs.br/jspui/handle/riufs/17526 |
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por |
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Pós-Graduação em Ciências Fisiológicas |
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Universidade Federal de Sergipe (UFS) |
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