Biotransformação de gingeróis por fungos endofíticos isoaldos de humirianthera ampla

• Autor(a) principal: Lisboa, Júlia Santana
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/12374
• topic: Gengibre; Fungos; Biocatálise; Zingiber officinale; Scytalidium lignicola; Scytalidium cuboideum; Biocatalysis

Fungi, because they present an enormous biodiversity, give rise to a great variety of these secondary metabolites, that have diverse functionalities. Its biotransformation study has been very useful in the production of new compounds, due to the increase of productivity of the desired substances, overcoming problems encountered in chemical analysis and the possibility of elucidation of the biosynthetic pathway. Thus, substances with broad therapeutic power, such as those isolated from Zingiber officinale, are being widely used in biocatalytic studies. Based on this, this work evaluated the biotransformation potential of two endophytic fungi isolated from the Humirianthera ampla plant against gingerols isolated from Zingiber officinale. The two fungal strains were submitted to molecular identification and cultured in Petri dishes in semisolid potato Agar Dextrose (BDA) for 7 days. For the biotransformation experiments, small pieces (˜ 6 mm) of each fungus were placed in the liquid culture media Dextrose Potato (BD), Meat Extract Medium (MEP) and Saboraud (SBD) for 7 days in growth. After the fungal growth, it was added the enriched fraction of gingerols (organic substrate), leaving under agitation for another 7 days. Experiments varying the growth days (7 °, 8 °, 9 °, 10 ° and 14 ° days) with the enriched fraction were also performed. At the end of the biotransformations, the extracts were obtained by liquid-liquid partition with ethyl acetate and analyzed by chromatographic methods. All procedures were performed in triplicate and with control experiments. The fungi were identified in Scytalidium lignicola and Scytalidium sp. For both fungi, the biotransformation was only observed in the medium of MEP growth and from the different chromatographic methods the appearance of a more apolar substance was observed in relation to the gingerols tested in all the studied days. This work has an unprecedented character since there are no papers in the literature on the isolation and identification of the endophytic fungi of the H. ampla nor the use of them in processes of biotransformation.