Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/6575 |
Resumo: | Orchids are ornamental plants cultivated by man long ago. The great diversity of colors and forms of the flowers of the species and natural and artificial hybrids adds to these plants high commercial value. The destruction of natural habitats, coupled with predation has led to the loss of genetic variability of the species, intensifying the already severe process of extinction.The aim of this study was to characterize native orchids of the SergipeState by ISSR molecular markers, as well as to study strategies for in vitro conservation under slow growth of three orchid species belonging to the subtribe Laeliinae. Two in vitro conservation experiments were realized, both in a completely randomized design. The first experiment was in a 3x3x2 factorial scheme, and we tested three combinations of carbon sources and osmotic regulators (20 g L-1of sucrose, 10 g L-1of sucrose + 5g L-1of mannitol, 10 g L-1of sucrose + 5g L-1of sorbitol), three orchid species (Epidendrum secundumJacq.,Encyclia dichroma (Lindl.) Schltr andCattleya tigrina A. Rich) and two temperatures (18 and 25°C).The second experiment was realized in a 4x3x2factorial scheme, and we tested four concentrations of salts of MS medium (100%, 75%, 50% and 25% of salts), three orchid species (Epidendrum secundumJacq.,Encyclia dichroma (Lindl.) Schltr andCattleya tigrina A. Rich) and two temperatures (18 and 25°C). The eight primers used in the study revealed a total of 87 fragments, all polymorphic, which proved effective in characterizing the collection of study. The diversity found within and between studied genera was high and promising for conservation studies.Cattleya tigrina presented 100% of survival at 180 days,and can be kept in medium with 25% of MS salts at 18°C. The temperature 18ºC also allowed the conservation of Epidendrum secundum for 365 days, with 100% of MS salts. Encyclia dichroma presented greater sensitivity to low temperature and can be kept at 25ºC with 25% of the salts of the MS medium for 365 days. |
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Santos, Mariana de SouzaBlank, Maria de Fátima Arrigonihttp://lattes.cnpq.br/98741519269626652017-10-02T12:49:28Z2017-10-02T12:49:28Z2014-02-19https://ri.ufs.br/handle/riufs/6575Orchids are ornamental plants cultivated by man long ago. The great diversity of colors and forms of the flowers of the species and natural and artificial hybrids adds to these plants high commercial value. The destruction of natural habitats, coupled with predation has led to the loss of genetic variability of the species, intensifying the already severe process of extinction.The aim of this study was to characterize native orchids of the SergipeState by ISSR molecular markers, as well as to study strategies for in vitro conservation under slow growth of three orchid species belonging to the subtribe Laeliinae. Two in vitro conservation experiments were realized, both in a completely randomized design. The first experiment was in a 3x3x2 factorial scheme, and we tested three combinations of carbon sources and osmotic regulators (20 g L-1of sucrose, 10 g L-1of sucrose + 5g L-1of mannitol, 10 g L-1of sucrose + 5g L-1of sorbitol), three orchid species (Epidendrum secundumJacq.,Encyclia dichroma (Lindl.) Schltr andCattleya tigrina A. Rich) and two temperatures (18 and 25°C).The second experiment was realized in a 4x3x2factorial scheme, and we tested four concentrations of salts of MS medium (100%, 75%, 50% and 25% of salts), three orchid species (Epidendrum secundumJacq.,Encyclia dichroma (Lindl.) Schltr andCattleya tigrina A. Rich) and two temperatures (18 and 25°C). The eight primers used in the study revealed a total of 87 fragments, all polymorphic, which proved effective in characterizing the collection of study. The diversity found within and between studied genera was high and promising for conservation studies.Cattleya tigrina presented 100% of survival at 180 days,and can be kept in medium with 25% of MS salts at 18°C. The temperature 18ºC also allowed the conservation of Epidendrum secundum for 365 days, with 100% of MS salts. Encyclia dichroma presented greater sensitivity to low temperature and can be kept at 25ºC with 25% of the salts of the MS medium for 365 days.As orquídeas são plantas ornamentais cultivadas pelo homemhá muito tempo. A grande diversidade de cores e formas das flores das espécies e híbridos naturais e artificiais agrega a estas plantas alto valor comercial. A destruição dos habitats naturais, aliada à coleta predatória, tem levado à perda da variabilidade genética das espécies, intensificando o já acentuado processo de extinção. O presente trabalho teve como objetivo caracterizar por marcadores moleculares tipo ISSR orquídeas nativas do Estado de Sergipe, bem como estudar estratégias para conservação in vitro,sob crescimento lento, para três espécies pertencentes à subtribo Laeliinae. Foram realizados dois experimentos de conservaçãoin vitro, ambos em delineamento inteiramente casualizado.O primeiro experimento foi em esquema fatorial 3x3x2, onde foram testadas três combinações de fontes de carbono e reguladores osmóticos (20 g L-1 de sacarose; 10 g L-1 de sacarose + 5g L-1 de manitol; 10 g L-1 de sacarose + 5g L-1 de sorbitol), três espécies de orquídea (Epidendrum secundum Jacq.,Encyclia dichroma (Lindl.) Schltr eCattleya tigrina A. Rich) e duas temperaturas (18 e 25ºC). O segundo experimento foi em esquema fatorial 4x3x2, onde foram testadas quatro concentrações de sais do meio MS (100%, 75%, 50% e 25% dos sais), três espécies de orquídea (Epidendrum secundum Jacq.,Encyclia dichroma (Lindl.) Schltr, eCattleya tigrina A.Rich) e duas temperaturas (18 e 25ºC). Os oito primers utilizados no trabalho revelaram um total de 87 fragmentos, todos polimórficos, que se mostraram eficientes na caracterização da coleção de estudo. A diversidade encontrada entre e dentro dos gêneros estudados mostrou-se elevada e promissora para estudos de conservação. Cattleya tigrina apresentou 100% de sobrevivência aos 180 dias podendo ser conservada em meio com 25% dos sais MS à 18ºC. Esta temperatura também permitiu a conservação de Epidendrum secundum por 365 dias em meio com 100% dos sais MS. Encyclia dichroma apresentou maior sensibilidade à baixa temperatura e pode ser conservada a 25ºC em meio com 25% dos sais MS por 365 dias.application/pdfporBotânicaOrquídeaOrquídea - CultivoGenética vegetalDiversidade genéticaISSRManitolSorbitolBotanyOrchidsOrchidsPlant geneticsGenetic diversityCNPQ::CIENCIAS AGRARIAS::AGRONOMIAProspecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em Agroecossistemasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSORIGINALMARIANA_SOUZA_SANTOS.pdfapplication/pdf2211204https://ri.ufs.br/jspui/bitstream/riufs/6575/1/MARIANA_SOUZA_SANTOS.pdf5f046ce9846d212a358e0416e0ec2436MD51TEXTMARIANA_SOUZA_SANTOS.pdf.txtMARIANA_SOUZA_SANTOS.pdf.txtExtracted texttext/plain137316https://ri.ufs.br/jspui/bitstream/riufs/6575/2/MARIANA_SOUZA_SANTOS.pdf.txt2462629c77ceab700a5b63fbba51851fMD52THUMBNAILMARIANA_SOUZA_SANTOS.pdf.jpgMARIANA_SOUZA_SANTOS.pdf.jpgGenerated Thumbnailimage/jpeg1286https://ri.ufs.br/jspui/bitstream/riufs/6575/3/MARIANA_SOUZA_SANTOS.pdf.jpge9cdb47f22313c64a93950fe816eb1c7MD53riufs/65752017-11-24 21:03:51.762oai:ufs.br:riufs/6575Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-25T00:03:51Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.por.fl_str_mv |
Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae) |
title |
Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae) |
spellingShingle |
Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae) Santos, Mariana de Souza Botânica Orquídea Orquídea - Cultivo Genética vegetal Diversidade genética ISSR Manitol Sorbitol Botany Orchids Orchids Plant genetics Genetic diversity CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae) |
title_full |
Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae) |
title_fullStr |
Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae) |
title_full_unstemmed |
Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae) |
title_sort |
Prospecção, caracterização molecular e conservação in vitro de Laeliinae (Orchidaceae) |
author |
Santos, Mariana de Souza |
author_facet |
Santos, Mariana de Souza |
author_role |
author |
dc.contributor.author.fl_str_mv |
Santos, Mariana de Souza |
dc.contributor.advisor1.fl_str_mv |
Blank, Maria de Fátima Arrigoni |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9874151926962665 |
contributor_str_mv |
Blank, Maria de Fátima Arrigoni |
dc.subject.por.fl_str_mv |
Botânica Orquídea Orquídea - Cultivo Genética vegetal Diversidade genética ISSR Manitol Sorbitol |
topic |
Botânica Orquídea Orquídea - Cultivo Genética vegetal Diversidade genética ISSR Manitol Sorbitol Botany Orchids Orchids Plant genetics Genetic diversity CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Botany Orchids Orchids Plant genetics Genetic diversity |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Orchids are ornamental plants cultivated by man long ago. The great diversity of colors and forms of the flowers of the species and natural and artificial hybrids adds to these plants high commercial value. The destruction of natural habitats, coupled with predation has led to the loss of genetic variability of the species, intensifying the already severe process of extinction.The aim of this study was to characterize native orchids of the SergipeState by ISSR molecular markers, as well as to study strategies for in vitro conservation under slow growth of three orchid species belonging to the subtribe Laeliinae. Two in vitro conservation experiments were realized, both in a completely randomized design. The first experiment was in a 3x3x2 factorial scheme, and we tested three combinations of carbon sources and osmotic regulators (20 g L-1of sucrose, 10 g L-1of sucrose + 5g L-1of mannitol, 10 g L-1of sucrose + 5g L-1of sorbitol), three orchid species (Epidendrum secundumJacq.,Encyclia dichroma (Lindl.) Schltr andCattleya tigrina A. Rich) and two temperatures (18 and 25°C).The second experiment was realized in a 4x3x2factorial scheme, and we tested four concentrations of salts of MS medium (100%, 75%, 50% and 25% of salts), three orchid species (Epidendrum secundumJacq.,Encyclia dichroma (Lindl.) Schltr andCattleya tigrina A. Rich) and two temperatures (18 and 25°C). The eight primers used in the study revealed a total of 87 fragments, all polymorphic, which proved effective in characterizing the collection of study. The diversity found within and between studied genera was high and promising for conservation studies.Cattleya tigrina presented 100% of survival at 180 days,and can be kept in medium with 25% of MS salts at 18°C. The temperature 18ºC also allowed the conservation of Epidendrum secundum for 365 days, with 100% of MS salts. Encyclia dichroma presented greater sensitivity to low temperature and can be kept at 25ºC with 25% of the salts of the MS medium for 365 days. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-02-19 |
dc.date.accessioned.fl_str_mv |
2017-10-02T12:49:28Z |
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2017-10-02T12:49:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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https://ri.ufs.br/handle/riufs/6575 |
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por |
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info:eu-repo/semantics/openAccess |
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Pós-Graduação em Agroecossistemas |
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