Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | http://ri.ufs.br/jspui/handle/riufs/17504 |
Resumo: | In this work, extraction optimization was performed by two techniques, ultrasound (UAE) and microwave (MAE), to obtain, simultaneously, extracts with better results in yield and total phenolic content, from Lippia alba and Lantana camara leaves, separately, using a Box-Behnken design and response surface methodology (RSM). The extracts of L. alba obtained under the optimum conditions by UAE (29% ethanol, 17.8 min and solvent to solid ratio of 71 mL/g) and MAE (43% ethanol, 15 min and microwave power of 600 W) resulted in yields of 20.33 and 26.94% and TPC of 30.86 and 41.13 mg EAG/g ms, respectively. Considering the species L. camara, the extracts obtained under the optimum conditions by UAE (35% ethanol, 25 min and solvent to solid ratio of 60:1 mL/g) and MAE (53% ethanol, 15 min and microwave power of 300 W) provided yields of 32.50 and 38.61% and TPC of 102.89 and 109.83 mg EAG/g ms, respectively. The extracts obtained by MAE from each species showed higher extraction yields, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities (DPPH, ABTS and FRAP) in relation to their respective conventional extracts (maceration, infusion, decoction and Soxhlet), highlighting the highest results presented by the species L. camara. The extracts obtained by UAE from each species showed higher values of TPC, TFC and antioxidant activities in relation to their respective maceration and infusion extracts. However, the extracts obtained from both species showed low cytotoxic activities against tumor cells and low enzymatic inhibition activities (AChE and BChE), indicating the need for further studies to evaluate their real biological potentials in relation to these activities. Through the analysis by Liquid Chromatography coupled to a Diode Array detector, it was possible to verify the presence of iridoids, phenolic acids, phenylpropanoids and flavonoids in the extracts of L. alba and L. camara, which corroborates the high antioxidant activities presented by the extracts of these species. |
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Jesus, Raphael Amancio deMoraes, Valéria Regina de Souza2023-05-04T16:43:57Z2023-05-04T16:43:57Z2022-10-31JESUS, Raphael Amancio de. Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. 2022. 213 f. Tese (Doutorado em Química) – Universidade Federal de Sergipe, São Cristóvão, 2022.http://ri.ufs.br/jspui/handle/riufs/17504In this work, extraction optimization was performed by two techniques, ultrasound (UAE) and microwave (MAE), to obtain, simultaneously, extracts with better results in yield and total phenolic content, from Lippia alba and Lantana camara leaves, separately, using a Box-Behnken design and response surface methodology (RSM). The extracts of L. alba obtained under the optimum conditions by UAE (29% ethanol, 17.8 min and solvent to solid ratio of 71 mL/g) and MAE (43% ethanol, 15 min and microwave power of 600 W) resulted in yields of 20.33 and 26.94% and TPC of 30.86 and 41.13 mg EAG/g ms, respectively. Considering the species L. camara, the extracts obtained under the optimum conditions by UAE (35% ethanol, 25 min and solvent to solid ratio of 60:1 mL/g) and MAE (53% ethanol, 15 min and microwave power of 300 W) provided yields of 32.50 and 38.61% and TPC of 102.89 and 109.83 mg EAG/g ms, respectively. The extracts obtained by MAE from each species showed higher extraction yields, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities (DPPH, ABTS and FRAP) in relation to their respective conventional extracts (maceration, infusion, decoction and Soxhlet), highlighting the highest results presented by the species L. camara. The extracts obtained by UAE from each species showed higher values of TPC, TFC and antioxidant activities in relation to their respective maceration and infusion extracts. However, the extracts obtained from both species showed low cytotoxic activities against tumor cells and low enzymatic inhibition activities (AChE and BChE), indicating the need for further studies to evaluate their real biological potentials in relation to these activities. Through the analysis by Liquid Chromatography coupled to a Diode Array detector, it was possible to verify the presence of iridoids, phenolic acids, phenylpropanoids and flavonoids in the extracts of L. alba and L. camara, which corroborates the high antioxidant activities presented by the extracts of these species.Neste trabalho foi realizada a otimização de extração por duas técnicas não convencionais, ultrassom (UAE) e micro-ondas (MAE), para a obtenção de extratos com melhores resultados de rendimento e conteúdo de fenólicos totais, de forma simultânea, a partir das folhas de Lippia alba e Lantana camara, separadamente, utilizando um planejamento Box-Behnken e metodologia de superfície de resposta (RSM). Os extratos de L. alba obtidos nas condições otimizadas por UAE (29% de etanol, 17,8 min e relação solvente para sólido de 71 mL/g) e MAE (43% de etanol, 15 min e potência de micro-ondas de 600 W) resultaram em rendimentos de 20,33 e 26,94% e TPC de 30,86 e 41,13 mg EAG/g ms, respectivamente. Considerando a espécie L. camara, os extratos obtidos nas condições otimizadas por UAE (35% de etanol, 25 min e relação solvente para sólido de 60:1 mL/g) e MAE (53% de etanol, 15 min e potência de micro-ondas de 300 W) forneceram rendimentos de 32,50 e 38,61% e TPC de 102,89 e 109,83 mg EAG/g ms, respectivamente. Os extratos obtidos por MAE a partir de cada espécie apresentaram maiores rendimentos de extração, conteúdo de fenólicos totais (TPC), conteúdo de flavonoides totais (TFC) e atividades antioxidantes (DPPH, ABTS e FRAP) em relação aos respectivos extratos convencionais (maceração, infusão, decocção e Soxhlet), com destaque para os maiores resultados apresentados pela espécie L. camara. Os extratos obtidos por UAE a partir de cada espécie apresentaram maiores valores de TPC, TFC e de atividades antioxidantes em relação aos seus respectivos extratos de maceração e infusão. No entanto, os extratos obtidos a partir das duas espécies apresentaram baixas atividades citotóxicas frente a células tumorais e baixas atividades de inibição enzimática (AChE e BChE), indicando a necessidade de estudos mais aprofundados para se avaliar os seus reais potenciais biológicos com relação a essas atividades. Através das análises por cromatografia líquida acoplada a detector de arranjo de diodos foi possível verificar a presença de iridoides, ácidos fenólicos, fenilpropanoides e flavonoides nos extratos de L. alba e L. camara, o que corrobora as elevadas atividades antioxidantes apresentadas pelos extratos dessas espécies.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSão CristóvãoporErva-cidreiraLantanaExtração (química)UltrassomMicro-ondasFenóisLippia albaLantana camaraExtração assistida por ultrassomExtração assistida por micro-ondasCompostos fenólicosUltrasound-assisted extractionMicrowave-assisted extractionPhenolic compoundsCIENCIAS EXATAS E DA TERRA::QUIMICAOtimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn.Optimization of ultrasound and microwave extraction of phenolic compounds from Lippia alba (Mill.) N. E. Brown and Lantana camara Linn. leavesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPós-Graduação em QuímicaUniversidade Federal de Sergipe (UFS)reponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/17504/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALRAPHAEL_AMANCIO_JESUS.pdfRAPHAEL_AMANCIO_JESUS.pdfapplication/pdf3266107https://ri.ufs.br/jspui/bitstream/riufs/17504/2/RAPHAEL_AMANCIO_JESUS.pdfeac6f91020f32c9ece11c7e3d7c9bcbeMD52TEXTRAPHAEL_AMANCIO_JESUS.pdf.txtRAPHAEL_AMANCIO_JESUS.pdf.txtExtracted texttext/plain345722https://ri.ufs.br/jspui/bitstream/riufs/17504/3/RAPHAEL_AMANCIO_JESUS.pdf.txt165410132fc2b379f105b27599f84f13MD53THUMBNAILRAPHAEL_AMANCIO_JESUS.pdf.jpgRAPHAEL_AMANCIO_JESUS.pdf.jpgGenerated Thumbnailimage/jpeg1448https://ri.ufs.br/jspui/bitstream/riufs/17504/4/RAPHAEL_AMANCIO_JESUS.pdf.jpgf03572d4b13f344fed613bdc7311dff8MD54riufs/175042023-05-04 13:44:11.62oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2023-05-04T16:44:11Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. |
dc.title.alternative.eng.fl_str_mv |
Optimization of ultrasound and microwave extraction of phenolic compounds from Lippia alba (Mill.) N. E. Brown and Lantana camara Linn. leaves |
title |
Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. |
spellingShingle |
Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. Jesus, Raphael Amancio de Erva-cidreira Lantana Extração (química) Ultrassom Micro-ondas Fenóis Lippia alba Lantana camara Extração assistida por ultrassom Extração assistida por micro-ondas Compostos fenólicos Ultrasound-assisted extraction Microwave-assisted extraction Phenolic compounds CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. |
title_full |
Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. |
title_fullStr |
Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. |
title_full_unstemmed |
Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. |
title_sort |
Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. |
author |
Jesus, Raphael Amancio de |
author_facet |
Jesus, Raphael Amancio de |
author_role |
author |
dc.contributor.author.fl_str_mv |
Jesus, Raphael Amancio de |
dc.contributor.advisor1.fl_str_mv |
Moraes, Valéria Regina de Souza |
contributor_str_mv |
Moraes, Valéria Regina de Souza |
dc.subject.por.fl_str_mv |
Erva-cidreira Lantana Extração (química) Ultrassom Micro-ondas Fenóis Lippia alba Lantana camara Extração assistida por ultrassom Extração assistida por micro-ondas Compostos fenólicos |
topic |
Erva-cidreira Lantana Extração (química) Ultrassom Micro-ondas Fenóis Lippia alba Lantana camara Extração assistida por ultrassom Extração assistida por micro-ondas Compostos fenólicos Ultrasound-assisted extraction Microwave-assisted extraction Phenolic compounds CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Ultrasound-assisted extraction Microwave-assisted extraction Phenolic compounds |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
In this work, extraction optimization was performed by two techniques, ultrasound (UAE) and microwave (MAE), to obtain, simultaneously, extracts with better results in yield and total phenolic content, from Lippia alba and Lantana camara leaves, separately, using a Box-Behnken design and response surface methodology (RSM). The extracts of L. alba obtained under the optimum conditions by UAE (29% ethanol, 17.8 min and solvent to solid ratio of 71 mL/g) and MAE (43% ethanol, 15 min and microwave power of 600 W) resulted in yields of 20.33 and 26.94% and TPC of 30.86 and 41.13 mg EAG/g ms, respectively. Considering the species L. camara, the extracts obtained under the optimum conditions by UAE (35% ethanol, 25 min and solvent to solid ratio of 60:1 mL/g) and MAE (53% ethanol, 15 min and microwave power of 300 W) provided yields of 32.50 and 38.61% and TPC of 102.89 and 109.83 mg EAG/g ms, respectively. The extracts obtained by MAE from each species showed higher extraction yields, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities (DPPH, ABTS and FRAP) in relation to their respective conventional extracts (maceration, infusion, decoction and Soxhlet), highlighting the highest results presented by the species L. camara. The extracts obtained by UAE from each species showed higher values of TPC, TFC and antioxidant activities in relation to their respective maceration and infusion extracts. However, the extracts obtained from both species showed low cytotoxic activities against tumor cells and low enzymatic inhibition activities (AChE and BChE), indicating the need for further studies to evaluate their real biological potentials in relation to these activities. Through the analysis by Liquid Chromatography coupled to a Diode Array detector, it was possible to verify the presence of iridoids, phenolic acids, phenylpropanoids and flavonoids in the extracts of L. alba and L. camara, which corroborates the high antioxidant activities presented by the extracts of these species. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-10-31 |
dc.date.accessioned.fl_str_mv |
2023-05-04T16:43:57Z |
dc.date.available.fl_str_mv |
2023-05-04T16:43:57Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
JESUS, Raphael Amancio de. Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. 2022. 213 f. Tese (Doutorado em Química) – Universidade Federal de Sergipe, São Cristóvão, 2022. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/17504 |
identifier_str_mv |
JESUS, Raphael Amancio de. Otimização de extração por ultrassom e micro-ondas de compostos fenólicos das folhas de Lippia alba (Mill.) N. E. Brown e Lantana camara Linn. 2022. 213 f. Tese (Doutorado em Química) – Universidade Federal de Sergipe, São Cristóvão, 2022. |
url |
http://ri.ufs.br/jspui/handle/riufs/17504 |
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Pós-Graduação em Química |
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Universidade Federal de Sergipe (UFS) |
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