Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/3760 |
Resumo: | Thyroid hormones are essential for normal brain development. Therefore thyroid disease may be responsible for the onset of severe neurological disorders. The proper functioning of the maternal thyroid gland, especially during the early stages of pregnancy, plays an important role in ensuring that the offspring have normal brain and body development.We aimed to investigate the impact of experimental gestational hypothyroidism (EGH) on nociceptive threshold and motor activity in the offspring at different postnatal ages (7th, 15th, 23rd, 30th, 60th and 120th postnatal days; PND) in rats. EGH was induced with methimazole (MMI) 0.02% in drinking water from day 9 of gestation until birth. The offspring was assessed for thermal and mechanical nociception using the tail-flick test and vonFrey filaments. Rota-Rod test and grip strength were used to assess motor function. EGH reduced thermal, but not mechanical threshold at all ages studied (p <0.05). Differently, 60 days old females offspring from MMI-treated dams (f-OMTD) were not affected by EGH when tested in the tail-flick. Only males OMTD presented a deficient locomotor performance using the Rota-Rod test (p < 0.01 at 60th PND), and the grip strength meter (p < 0.05 at 120th PND), in comparison to offspring from water-treated dams (OWTD). In conclusion, EGH promotes hypersensitivity to noxious thermal, but not mechanical stimulus, whereas motor performance is reduced and thermal hypernociception remains present, both only in mature males OMTD, suggesting the presence of a protective factor in females. |
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Alves, Iura Gonzalez Nogueirahttp://lattes.cnpq.br/3659358368327255Santana Filho, Valter Joviniano dehttp://lattes.cnpq.br/95457709071604352017-09-26T12:17:11Z2017-09-26T12:17:11Z2012-01-24https://ri.ufs.br/handle/riufs/3760Thyroid hormones are essential for normal brain development. Therefore thyroid disease may be responsible for the onset of severe neurological disorders. The proper functioning of the maternal thyroid gland, especially during the early stages of pregnancy, plays an important role in ensuring that the offspring have normal brain and body development.We aimed to investigate the impact of experimental gestational hypothyroidism (EGH) on nociceptive threshold and motor activity in the offspring at different postnatal ages (7th, 15th, 23rd, 30th, 60th and 120th postnatal days; PND) in rats. EGH was induced with methimazole (MMI) 0.02% in drinking water from day 9 of gestation until birth. The offspring was assessed for thermal and mechanical nociception using the tail-flick test and vonFrey filaments. Rota-Rod test and grip strength were used to assess motor function. EGH reduced thermal, but not mechanical threshold at all ages studied (p <0.05). Differently, 60 days old females offspring from MMI-treated dams (f-OMTD) were not affected by EGH when tested in the tail-flick. Only males OMTD presented a deficient locomotor performance using the Rota-Rod test (p < 0.01 at 60th PND), and the grip strength meter (p < 0.05 at 120th PND), in comparison to offspring from water-treated dams (OWTD). In conclusion, EGH promotes hypersensitivity to noxious thermal, but not mechanical stimulus, whereas motor performance is reduced and thermal hypernociception remains present, both only in mature males OMTD, suggesting the presence of a protective factor in females.Os hormônios tireoideanos são essenciais para o desenvolvimento normal do cérebro. Portanto, doenças da tireóide podem ser responsáveis pelo aparecimento de graves distúrbios neurológicos. O bom funcionamento da glândula tireóide materna, especialmente durante os primeiros meses da gravidez, desempenha um papel importante para garantir que os filhotes apresentem adequado desenvolvimento corporal e cerebral. No presente estudo, investigou-se o impacto do hipotireoidismo gestacional experimental (HGE) na sensibiliade mecânica e térmica e desempenho locomotor na prole em diferentes idades pós-natais (7, 15, 23, 30, 60 e 120 dias pós-natais; DPNs). O HGE foi induzido adicionando metimazol (MTZ), a uma concentração de 0,02%, em água potável a partir do dia 9 de gestação até o parto. A prole foi avaliada para sensibilidade térmica e mecânica através dos testes tail-flick e vonFrey, respectivamente. Os testes Rota-Rod e o Grip Strength Meter foram utilizados para avaliar a função motora da prole. Assim, nossos dados demonstraram que o HGE não alterou a sensibilidade mecânica, no entanto, reduziu a latência térmica até o 1º mês de vida dos filhotes (7, 15, 23 e 30 DPN). Aos 60 DPNs, somente os filhotes machos de mães tratadas com MTZ durante o período gestacional apresentaram redução na latência térmica (p<0,05). Acrescido a isto, estes mesmos machos apresentaram desempenho locomotor deficiente aos 60 DPNs e força de preensão reduzida aos 120 DPNs (p<0,01 e p<0,05, respectivamente), em comparação com filhotes de mães eutireóideas. As mesmas alterações não foram observadas na prole do sexo feminino. Em conclusão, o HGE não altera a sensibilidade mecânica da prole, porém promove hipersensibilidade a estímulos térmicos nocivos, sendo que, na fase adulta (60 DPN), apenas os machos são afetados. Ademais, o desempenho motor é reduzido somente em machos, sugerindo a presença de algum fator de proteção no sexo feminino.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de SergipePós-Graduação em Ciências da SaúdeUFSBRHipotireoidismo congênitoNocicepçãoRatosDiferenças sexuaisControle motorCongenital hypothyroidismNociceptionSexual dimorphismRatsMotor controlCNPQ::CIENCIAS DA SAUDEInfluência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratosINFLUENCE OF EXPERIMENTAL HYPOTHYROIDISM DURING PREGNANCY IN NOCICEPTION AND MOTOR PERFORMANCE IN OFFSPRING OF RATS,info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSTEXTIURA_GONZALEZ_NOGUEIRA_ALVES.pdf.txtIURA_GONZALEZ_NOGUEIRA_ALVES.pdf.txtExtracted texttext/plain225360https://ri.ufs.br/jspui/bitstream/riufs/3760/2/IURA_GONZALEZ_NOGUEIRA_ALVES.pdf.txta821ad65b707abd457a8c2fe0f111f86MD52THUMBNAILIURA_GONZALEZ_NOGUEIRA_ALVES.pdf.jpgIURA_GONZALEZ_NOGUEIRA_ALVES.pdf.jpgGenerated Thumbnailimage/jpeg1306https://ri.ufs.br/jspui/bitstream/riufs/3760/3/IURA_GONZALEZ_NOGUEIRA_ALVES.pdf.jpgc6fb6c059455e9f3c817c7341380dfa1MD53ORIGINALIURA_GONZALEZ_NOGUEIRA_ALVES.pdfapplication/pdf1668979https://ri.ufs.br/jspui/bitstream/riufs/3760/1/IURA_GONZALEZ_NOGUEIRA_ALVES.pdff0524e2d7df757d787f9c40c0b933128MD51riufs/37602017-11-28 16:50:09.775oai:ufs.br:riufs/3760Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-28T19:50:09Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.por.fl_str_mv |
Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos |
dc.title.alternative.eng.fl_str_mv |
INFLUENCE OF EXPERIMENTAL HYPOTHYROIDISM DURING PREGNANCY IN NOCICEPTION AND MOTOR PERFORMANCE IN OFFSPRING OF RATS, |
title |
Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos |
spellingShingle |
Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos Alves, Iura Gonzalez Nogueira Hipotireoidismo congênito Nocicepção Ratos Diferenças sexuais Controle motor Congenital hypothyroidism Nociception Sexual dimorphism Rats Motor control CNPQ::CIENCIAS DA SAUDE |
title_short |
Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos |
title_full |
Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos |
title_fullStr |
Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos |
title_full_unstemmed |
Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos |
title_sort |
Influência do hipotireoidismo gestacional experimental na nocicepção e desempenho motor da prole de ratos |
author |
Alves, Iura Gonzalez Nogueira |
author_facet |
Alves, Iura Gonzalez Nogueira |
author_role |
author |
dc.contributor.author.fl_str_mv |
Alves, Iura Gonzalez Nogueira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3659358368327255 |
dc.contributor.advisor1.fl_str_mv |
Santana Filho, Valter Joviniano de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9545770907160435 |
contributor_str_mv |
Santana Filho, Valter Joviniano de |
dc.subject.por.fl_str_mv |
Hipotireoidismo congênito Nocicepção Ratos Diferenças sexuais Controle motor |
topic |
Hipotireoidismo congênito Nocicepção Ratos Diferenças sexuais Controle motor Congenital hypothyroidism Nociception Sexual dimorphism Rats Motor control CNPQ::CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
Congenital hypothyroidism Nociception Sexual dimorphism Rats Motor control |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE |
description |
Thyroid hormones are essential for normal brain development. Therefore thyroid disease may be responsible for the onset of severe neurological disorders. The proper functioning of the maternal thyroid gland, especially during the early stages of pregnancy, plays an important role in ensuring that the offspring have normal brain and body development.We aimed to investigate the impact of experimental gestational hypothyroidism (EGH) on nociceptive threshold and motor activity in the offspring at different postnatal ages (7th, 15th, 23rd, 30th, 60th and 120th postnatal days; PND) in rats. EGH was induced with methimazole (MMI) 0.02% in drinking water from day 9 of gestation until birth. The offspring was assessed for thermal and mechanical nociception using the tail-flick test and vonFrey filaments. Rota-Rod test and grip strength were used to assess motor function. EGH reduced thermal, but not mechanical threshold at all ages studied (p <0.05). Differently, 60 days old females offspring from MMI-treated dams (f-OMTD) were not affected by EGH when tested in the tail-flick. Only males OMTD presented a deficient locomotor performance using the Rota-Rod test (p < 0.01 at 60th PND), and the grip strength meter (p < 0.05 at 120th PND), in comparison to offspring from water-treated dams (OWTD). In conclusion, EGH promotes hypersensitivity to noxious thermal, but not mechanical stimulus, whereas motor performance is reduced and thermal hypernociception remains present, both only in mature males OMTD, suggesting the presence of a protective factor in females. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-01-24 |
dc.date.accessioned.fl_str_mv |
2017-09-26T12:17:11Z |
dc.date.available.fl_str_mv |
2017-09-26T12:17:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/3760 |
url |
https://ri.ufs.br/handle/riufs/3760 |
dc.language.iso.fl_str_mv |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Sergipe |
dc.publisher.program.fl_str_mv |
Pós-Graduação em Ciências da Saúde |
dc.publisher.initials.fl_str_mv |
UFS |
dc.publisher.country.fl_str_mv |
BR |
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Universidade Federal de Sergipe |
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Universidade Federal de Sergipe (UFS) |
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