Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/3035 |
Resumo: | Leaf-cutting ants of Atta and Acromyrmex genus (Hymenoptera: Formicidae) are common insects in the Neotropics region and an important pests in agricultural environments. These organisms are often controlled using organosintetics insecticides, however, mostly products are inefficient. This problem has generated a growing demand for environmentally safe products to control these pests. Thus, in order to contribute with new methods of control, the aim of this work was to evaluate the ant activity of essential oil of A. trilobata and its major compounds on cutting ants Atta sexdens and Acromyrmex balzani. Initially, it was performed toxicity bioassays by two different routes of exposure - contact and fumigation. Once determined the exposure way with the best ant activity, this route was choosed to performe the next experiments, bioassay toxicity (lethal concentration and time), effects of binary mixtures (synergistic, additive and antagonistic) of major compounds, repellency and irritability. The essential oil of A. trilobata was obtained by hydrodistillation of the dried stem and the identification / quantification of compounds was performed by GC/MS/FID. Twenty Five compounds were identified in the essential oil of A. trilobata, that are mostly composed of monoterpenes. The major constituents were the monoterpenes: sulcatyl acetate (25.64%), limonene (24.80%), p-cymene (10.41%) and linalool (9.51%). The essential oil of A. trilobata and their major compounds were effective against workers of A. balzani and A. sexdens when the route of exposure was fumigation. These compounds have demonstrated efficient activity on ant species A. balzani and A. sexdens, with LCs50 ranging from 2.18 to 6.73 uL mL-1. The monoterpenes linalool and sulcatyl acetate when applied alone were 1.6 and 1.7 times more toxic than the essential oil against A. balzani. As for A. sexdens, p-cymene was 1.6 times more toxic than the essential oil of A. trilobata. Workers of A. sexdens were more tolerant to the essential oil, linalool and sulcatyl acetate. The essential oil of A. trilobata and its major compounds acted faster on A. sexdens compared to A. balzani. On average, these compounds took 29.3 and 11.3 hours to cause mortality in half population of A. balzani and A. sexdens, respectively. The major compounds of the essential oil of A. trilobata acted synergistically in toxicity of A. balzani. As for A. sexdens most combinations of the compounds exhibited an additive effect. The essential oil of A. trilobata and its major compounds were repellent and caused great irritability to workers of A. balzani and A. sexdens. Thus, our results show a great potential of the essential oil of A. trilobata and its major compounds for develop new insecticides. |
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Oliveira, Bruna Maria Santos dehttp://lattes.cnpq.br/6928253758100662Bacci, Leandrohttp://lattes.cnpq.br/44045491177522922017-09-25T13:25:07Z2017-09-25T13:25:07Z2015-02-27OLIVEIRA, Bruna Maria Santos de. Ant activity of Aristolochia trilobata L. (Aristolochiaceae) on leaf-cutting ants. 2015. 66 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Sergipe, São Cristóvão, 2015.https://ri.ufs.br/handle/riufs/3035Leaf-cutting ants of Atta and Acromyrmex genus (Hymenoptera: Formicidae) are common insects in the Neotropics region and an important pests in agricultural environments. These organisms are often controlled using organosintetics insecticides, however, mostly products are inefficient. This problem has generated a growing demand for environmentally safe products to control these pests. Thus, in order to contribute with new methods of control, the aim of this work was to evaluate the ant activity of essential oil of A. trilobata and its major compounds on cutting ants Atta sexdens and Acromyrmex balzani. Initially, it was performed toxicity bioassays by two different routes of exposure - contact and fumigation. Once determined the exposure way with the best ant activity, this route was choosed to performe the next experiments, bioassay toxicity (lethal concentration and time), effects of binary mixtures (synergistic, additive and antagonistic) of major compounds, repellency and irritability. The essential oil of A. trilobata was obtained by hydrodistillation of the dried stem and the identification / quantification of compounds was performed by GC/MS/FID. Twenty Five compounds were identified in the essential oil of A. trilobata, that are mostly composed of monoterpenes. The major constituents were the monoterpenes: sulcatyl acetate (25.64%), limonene (24.80%), p-cymene (10.41%) and linalool (9.51%). The essential oil of A. trilobata and their major compounds were effective against workers of A. balzani and A. sexdens when the route of exposure was fumigation. These compounds have demonstrated efficient activity on ant species A. balzani and A. sexdens, with LCs50 ranging from 2.18 to 6.73 uL mL-1. The monoterpenes linalool and sulcatyl acetate when applied alone were 1.6 and 1.7 times more toxic than the essential oil against A. balzani. As for A. sexdens, p-cymene was 1.6 times more toxic than the essential oil of A. trilobata. Workers of A. sexdens were more tolerant to the essential oil, linalool and sulcatyl acetate. The essential oil of A. trilobata and its major compounds acted faster on A. sexdens compared to A. balzani. On average, these compounds took 29.3 and 11.3 hours to cause mortality in half population of A. balzani and A. sexdens, respectively. The major compounds of the essential oil of A. trilobata acted synergistically in toxicity of A. balzani. As for A. sexdens most combinations of the compounds exhibited an additive effect. The essential oil of A. trilobata and its major compounds were repellent and caused great irritability to workers of A. balzani and A. sexdens. Thus, our results show a great potential of the essential oil of A. trilobata and its major compounds for develop new insecticides.As formigas cortadeiras dos gêneros Atta e Acromyrmex (Hymenoptera: Formicidae) são insetos comuns na região neotropical e importantes pragas em ambientes agrícolas. Estes organismos são frequentemente controlados com o uso de inseticidas organossintéticos, no entanto, este método de controle é muitas vezes ineficiente. Isso tem gerado uma demanda crescente na busca de alternativas de controle que sejam ambientalmente sustentáveis. Assim, com o intuito de contribuir no controle, objetivou-se com este trabalho avaliar a atividade formicida do óleo essencial de Aristolochia trilobata e de seus compostos majoritários sobre as formigas cortadeiras Atta sexdens e Acromyrmex balzani. Para isso, foram realizados inicialmente bioensaios de toxicidade por duas diferentes vias de exposição - contato e fumigação. Uma vez determinada a via de exposição com melhor atividade formicida foram realizados, por esta via de exposição, bioensaios de toxicidade (concentração e tempo letal), efeitos das misturas binárias (sinergismo, aditismo e antagonismo) dos compostos majoritários, repelência e irritabilidade. O óleo essencial de A. trilobata foi obtido por hidrodestilação do caule seco e a identificação/quantificação dos compostos foi realizada por CG/EM/DIC. Foram identificados 25 compostos no óleo essencial de A. trilobata, sendo a sua maioria formada por monoterpenos. Os constituintes em maiores proporções foram os monoterpenos: acetato de sulcatila (25,64%), limoneno (24,80%), p-cimeno (10,41%) e linalol (9,51%). O óleo essencial de A. trilobata e seus compostos majoritários isolados foram eficientes contra operárias de A. balzani e A. sexdens quando a via de exposição foi fumigação. Estes compostos demonstraram eficiente atividade formicida sobre as espécies, com CLs50 variando de 2,18 a 6,73 μL mL-1. Os monoterpenos linalol e acetato de sulcatila quando aplicados isoladamente foram 1,6 e 1,7 vezes mais tóxicos do que o óleo essencial de A. trilobata para A. balzani. Já para A. sexdens, o composto p-cimeno foi 1,6 vezes mais tóxico do que o óleo essencial. Operárias de A. sexdens foram mais tolerantes ao óleo essencial de A. trilobata e aos compostos linalol e acetato de sulcatila. O óleo essencial de A. trilobata e seus compostos majoritários atuaram de forma mais rápida sobre A. sexdens em comparação a A. balzani. Na média, estes compostos demoraram 29,3 e 11,3 horas para causar mortalidade na metade das populações de A. balzani e A. sexdens, respectivamente. Os compostos majoritários do óleo essencial de A. trilobata atuaram de forma sinérgica na toxicidade sobre A. balzani. Já para a espécie A. sexdens a maioria das combinações entre os compostos exibiu efeito aditivo. O óleo essencial de A. trilobata e seus compostos majoritários foram repelentes e causaram grande irritabilidade às operárias de A. balzani e A. sexdens. Assim, nossos resultados mostram o grande potencial do óleo essencial de A. trilobata e seus compostos majoritários para o desenvolvimento de novos formicidas.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de SergipePós-Graduação em Agricultura e BiodiversidadeUFSBRPlantas inseticidasÓleos essenciaisFormicidaeFumigaçãoSinergismo e repelênciaAgrobiodiversidadeAristoloquiáceaFormigaFormiga-cortadeiraInseticidasInsecticide plantsEssential oilsFormicidaeFumigationSynergismRepellencyCNPQ::CIENCIAS AGRARIAS::AGRONOMIAAtividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeirasAnt activity of Aristolochia trilobata L. (Aristolochiaceae) on leaf-cutting antsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSORIGINALBRUNA_MARIA_SANTOS_OLIVEIRA.pdfapplication/pdf591044https://ri.ufs.br/jspui/bitstream/riufs/3035/1/BRUNA_MARIA_SANTOS_OLIVEIRA.pdfd3a70609231e6231e9dbd52fb954aeedMD51TEXTBRUNA_MARIA_SANTOS_OLIVEIRA.pdf.txtBRUNA_MARIA_SANTOS_OLIVEIRA.pdf.txtExtracted texttext/plain117404https://ri.ufs.br/jspui/bitstream/riufs/3035/2/BRUNA_MARIA_SANTOS_OLIVEIRA.pdf.txtfd2a7a026b2f1c85c2f0ac7ec5af8772MD52THUMBNAILBRUNA_MARIA_SANTOS_OLIVEIRA.pdf.jpgBRUNA_MARIA_SANTOS_OLIVEIRA.pdf.jpgGenerated Thumbnailimage/jpeg1306https://ri.ufs.br/jspui/bitstream/riufs/3035/3/BRUNA_MARIA_SANTOS_OLIVEIRA.pdf.jpg33520315c871ab3303a06501e520e294MD53riufs/30352017-11-24 21:12:31.29oai:ufs.br:riufs/3035Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-25T00:12:31Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.por.fl_str_mv |
Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras |
dc.title.alternative.eng.fl_str_mv |
Ant activity of Aristolochia trilobata L. (Aristolochiaceae) on leaf-cutting ants |
title |
Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras |
spellingShingle |
Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras Oliveira, Bruna Maria Santos de Plantas inseticidas Óleos essenciais Formicidae Fumigação Sinergismo e repelência Agrobiodiversidade Aristoloquiácea Formiga Formiga-cortadeira Inseticidas Insecticide plants Essential oils Formicidae Fumigation Synergism Repellency CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras |
title_full |
Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras |
title_fullStr |
Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras |
title_full_unstemmed |
Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras |
title_sort |
Atividade formicida de Aristolochia trilobata L. (Aristolochiaceae) sobre formigas cortadeiras |
author |
Oliveira, Bruna Maria Santos de |
author_facet |
Oliveira, Bruna Maria Santos de |
author_role |
author |
dc.contributor.author.fl_str_mv |
Oliveira, Bruna Maria Santos de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6928253758100662 |
dc.contributor.advisor1.fl_str_mv |
Bacci, Leandro |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4404549117752292 |
contributor_str_mv |
Bacci, Leandro |
dc.subject.por.fl_str_mv |
Plantas inseticidas Óleos essenciais Formicidae Fumigação Sinergismo e repelência Agrobiodiversidade Aristoloquiácea Formiga Formiga-cortadeira Inseticidas |
topic |
Plantas inseticidas Óleos essenciais Formicidae Fumigação Sinergismo e repelência Agrobiodiversidade Aristoloquiácea Formiga Formiga-cortadeira Inseticidas Insecticide plants Essential oils Formicidae Fumigation Synergism Repellency CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Insecticide plants Essential oils Formicidae Fumigation Synergism Repellency |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Leaf-cutting ants of Atta and Acromyrmex genus (Hymenoptera: Formicidae) are common insects in the Neotropics region and an important pests in agricultural environments. These organisms are often controlled using organosintetics insecticides, however, mostly products are inefficient. This problem has generated a growing demand for environmentally safe products to control these pests. Thus, in order to contribute with new methods of control, the aim of this work was to evaluate the ant activity of essential oil of A. trilobata and its major compounds on cutting ants Atta sexdens and Acromyrmex balzani. Initially, it was performed toxicity bioassays by two different routes of exposure - contact and fumigation. Once determined the exposure way with the best ant activity, this route was choosed to performe the next experiments, bioassay toxicity (lethal concentration and time), effects of binary mixtures (synergistic, additive and antagonistic) of major compounds, repellency and irritability. The essential oil of A. trilobata was obtained by hydrodistillation of the dried stem and the identification / quantification of compounds was performed by GC/MS/FID. Twenty Five compounds were identified in the essential oil of A. trilobata, that are mostly composed of monoterpenes. The major constituents were the monoterpenes: sulcatyl acetate (25.64%), limonene (24.80%), p-cymene (10.41%) and linalool (9.51%). The essential oil of A. trilobata and their major compounds were effective against workers of A. balzani and A. sexdens when the route of exposure was fumigation. These compounds have demonstrated efficient activity on ant species A. balzani and A. sexdens, with LCs50 ranging from 2.18 to 6.73 uL mL-1. The monoterpenes linalool and sulcatyl acetate when applied alone were 1.6 and 1.7 times more toxic than the essential oil against A. balzani. As for A. sexdens, p-cymene was 1.6 times more toxic than the essential oil of A. trilobata. Workers of A. sexdens were more tolerant to the essential oil, linalool and sulcatyl acetate. The essential oil of A. trilobata and its major compounds acted faster on A. sexdens compared to A. balzani. On average, these compounds took 29.3 and 11.3 hours to cause mortality in half population of A. balzani and A. sexdens, respectively. The major compounds of the essential oil of A. trilobata acted synergistically in toxicity of A. balzani. As for A. sexdens most combinations of the compounds exhibited an additive effect. The essential oil of A. trilobata and its major compounds were repellent and caused great irritability to workers of A. balzani and A. sexdens. Thus, our results show a great potential of the essential oil of A. trilobata and its major compounds for develop new insecticides. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-27 |
dc.date.accessioned.fl_str_mv |
2017-09-25T13:25:07Z |
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2017-09-25T13:25:07Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Bruna Maria Santos de. Ant activity of Aristolochia trilobata L. (Aristolochiaceae) on leaf-cutting ants. 2015. 66 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Sergipe, São Cristóvão, 2015. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/3035 |
identifier_str_mv |
OLIVEIRA, Bruna Maria Santos de. Ant activity of Aristolochia trilobata L. (Aristolochiaceae) on leaf-cutting ants. 2015. 66 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Sergipe, São Cristóvão, 2015. |
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https://ri.ufs.br/handle/riufs/3035 |
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Universidade Federal de Sergipe |
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Pós-Graduação em Agricultura e Biodiversidade |
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UFS |
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BR |
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Universidade Federal de Sergipe |
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