Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica

Detalhes bibliográficos
Autor(a) principal: Maciel, Leonardo Yung dos Santos
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/3761
Resumo: Repeated injections of acidic saline into the gastrocnemius muscle in rats produce a bilateral long-lasting hiperalgesia similar to fibromyalgia syndrome in humans. After the induction, it´s possible to test the efficacy of analgesic strategies such as electroacupuncture and acupuncture. This study aimed to identify the effects of acupuncture and electroacupuncture in the hyperalgesia produced by repeated intramuscular injections of acidic saline. Twenty four male Wistar rats were divided into four groups (n = 6, in each group): control, acupuncture, electroacupuncture (EA) 15 Hz and (EA) 100 Hz. Left gastrocnemius muscle from all animals was injected with 100 μL of pH 4.0 sterile saline twice five days apart. Electroacupuncture, acupuncture or control was daily administered (20 min) for 5 consecutive days under isoflurane anesthesia (2% 5%). Needles were placed in the Zuzanli (St36) and Sanyinjiao (SP6). We assessed the secondary mechanical hyperalgesia, thermal hyperalgesia and motor performance before the two injections, and before and after the treatment each day. The results indicate that there was a significant reduction of both mechanical withdrawal threshold and paw withdrawal latency 24 hours following the second injection. Therefore, both mechanical and thermal hyperalgesia were significantly reversed by EA 15 Hz, EA 100 Hz and acupuncture. It can be suggested that as high and low frequency eletroacupuncture as acupuncture is effective in hiperalgesia reducing in this model of chronic muscle pain.
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spelling Maciel, Leonardo Yung dos Santoshttp://lattes.cnpq.br/9819654988177433Santana, Josimari Melo dehttp://lattes.cnpq.br/34369255129515082017-09-26T12:17:11Z2017-09-26T12:17:11Z2012-01-25https://ri.ufs.br/handle/riufs/3761Repeated injections of acidic saline into the gastrocnemius muscle in rats produce a bilateral long-lasting hiperalgesia similar to fibromyalgia syndrome in humans. After the induction, it´s possible to test the efficacy of analgesic strategies such as electroacupuncture and acupuncture. This study aimed to identify the effects of acupuncture and electroacupuncture in the hyperalgesia produced by repeated intramuscular injections of acidic saline. Twenty four male Wistar rats were divided into four groups (n = 6, in each group): control, acupuncture, electroacupuncture (EA) 15 Hz and (EA) 100 Hz. Left gastrocnemius muscle from all animals was injected with 100 μL of pH 4.0 sterile saline twice five days apart. Electroacupuncture, acupuncture or control was daily administered (20 min) for 5 consecutive days under isoflurane anesthesia (2% 5%). Needles were placed in the Zuzanli (St36) and Sanyinjiao (SP6). We assessed the secondary mechanical hyperalgesia, thermal hyperalgesia and motor performance before the two injections, and before and after the treatment each day. The results indicate that there was a significant reduction of both mechanical withdrawal threshold and paw withdrawal latency 24 hours following the second injection. Therefore, both mechanical and thermal hyperalgesia were significantly reversed by EA 15 Hz, EA 100 Hz and acupuncture. It can be suggested that as high and low frequency eletroacupuncture as acupuncture is effective in hiperalgesia reducing in this model of chronic muscle pain.Repetidas injeções de salina ácida no músculo gastrocnêmio de ratos produzem uma hiperalgesia bilateral de longa duração semelhante a fibromialgia em humanos. Após esta indução, é possível analisar a eficácia de modalidades analgésicas como a acupuntura e eletroacupuntura. O objetivo deste estudo foi identificar o efeito da eletroacupuntura e acupuntura na hiperalgesia bilateral produzida por dupla injeção intramuscular de salina ácida. Foram utilizados 24 ratos Wistar machos, divididos em quatro grupos: controle, acupuntura, eletroacupuntura (EA) de 15 Hz e EA de 100 Hz. No músculo gastrocnêmio esquerdo de todos os animais, foi injetado um volume de 100 μL de solução salina estéril pH 4,0 em duas ocasiões, com intervalo de 5 dias entre elas. O tratamento com EA foi feito diariamente por 5 dias consecutivos, com duração de 20 minutos. Os animais foram anestesiados com isoflurano (2% -5%) para possibilitar a intervenção. As agulhas foram colocadas nos pontos Zuzanli (St36) e Sanyinjiao (SP6). Foram avaliados a hiperalgesia mecânica secundária, hiperalgesia térmica e desempenho motor antes das duas injeções, e antes e após o tratamento. Os resultados indicam que ocorreu uma redução significativa do limiar mecânico e da latência térmica 24 horas após a segunda injeção. Além disso, houve reversão significativa da hiperalgesia mecânica e térmica após administração de EA 15 Hz, EA 100 Hz e acupuntura. Estes dados sugerem que tanto a EA em alta ou baixa freqüência quanto a acupuntura são eficazes na redução da hiperalgesia neste modelo de dor muscular crônica.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de SergipePós-Graduação em Ciências da SaúdeUFSBRHiperalgesiaDorEletroacupunturaFibromialgiaAnalgesiaRatosHyperalgesiaPainFibromyalgiaElectroacupunctureAnalgesiaRatCNPQ::CIENCIAS DA SAUDEEletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônicaELECTROACUPUNCTURE AND ACUPUNCTURE REDUCES HYPERALGESIA PRODUCED BY REPEATED INTRAMUSCULAR INJECTIONS OF ACIDIC SALINE IN RATS.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSTEXTLEONARDO_YUNG_SANTOS_MACIEL.pdf.txtLEONARDO_YUNG_SANTOS_MACIEL.pdf.txtExtracted texttext/plain85938https://ri.ufs.br/jspui/bitstream/riufs/3761/2/LEONARDO_YUNG_SANTOS_MACIEL.pdf.txt9d78910055bb06286206f2f033701c74MD52THUMBNAILLEONARDO_YUNG_SANTOS_MACIEL.pdf.jpgLEONARDO_YUNG_SANTOS_MACIEL.pdf.jpgGenerated Thumbnailimage/jpeg1499https://ri.ufs.br/jspui/bitstream/riufs/3761/3/LEONARDO_YUNG_SANTOS_MACIEL.pdf.jpg8a9bff025522de1324e987cdbe92b65aMD53ORIGINALLEONARDO_YUNG_SANTOS_MACIEL.pdfapplication/pdf931646https://ri.ufs.br/jspui/bitstream/riufs/3761/1/LEONARDO_YUNG_SANTOS_MACIEL.pdf72e7a81d8a73c69afb97d4b164a218e1MD51riufs/37612017-11-28 16:38:00.545oai:ufs.br:riufs/3761Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-28T19:38Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.por.fl_str_mv Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
dc.title.alternative.eng.fl_str_mv ELECTROACUPUNCTURE AND ACUPUNCTURE REDUCES HYPERALGESIA PRODUCED BY REPEATED INTRAMUSCULAR INJECTIONS OF ACIDIC SALINE IN RATS.
title Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
spellingShingle Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
Maciel, Leonardo Yung dos Santos
Hiperalgesia
Dor
Eletroacupuntura
Fibromialgia
Analgesia
Ratos
Hyperalgesia
Pain
Fibromyalgia
Electroacupuncture
Analgesia
Rat
CNPQ::CIENCIAS DA SAUDE
title_short Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
title_full Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
title_fullStr Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
title_full_unstemmed Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
title_sort Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
author Maciel, Leonardo Yung dos Santos
author_facet Maciel, Leonardo Yung dos Santos
author_role author
dc.contributor.author.fl_str_mv Maciel, Leonardo Yung dos Santos
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9819654988177433
dc.contributor.advisor1.fl_str_mv Santana, Josimari Melo de
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3436925512951508
contributor_str_mv Santana, Josimari Melo de
dc.subject.por.fl_str_mv Hiperalgesia
Dor
Eletroacupuntura
Fibromialgia
Analgesia
Ratos
topic Hiperalgesia
Dor
Eletroacupuntura
Fibromialgia
Analgesia
Ratos
Hyperalgesia
Pain
Fibromyalgia
Electroacupuncture
Analgesia
Rat
CNPQ::CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv Hyperalgesia
Pain
Fibromyalgia
Electroacupuncture
Analgesia
Rat
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE
description Repeated injections of acidic saline into the gastrocnemius muscle in rats produce a bilateral long-lasting hiperalgesia similar to fibromyalgia syndrome in humans. After the induction, it´s possible to test the efficacy of analgesic strategies such as electroacupuncture and acupuncture. This study aimed to identify the effects of acupuncture and electroacupuncture in the hyperalgesia produced by repeated intramuscular injections of acidic saline. Twenty four male Wistar rats were divided into four groups (n = 6, in each group): control, acupuncture, electroacupuncture (EA) 15 Hz and (EA) 100 Hz. Left gastrocnemius muscle from all animals was injected with 100 μL of pH 4.0 sterile saline twice five days apart. Electroacupuncture, acupuncture or control was daily administered (20 min) for 5 consecutive days under isoflurane anesthesia (2% 5%). Needles were placed in the Zuzanli (St36) and Sanyinjiao (SP6). We assessed the secondary mechanical hyperalgesia, thermal hyperalgesia and motor performance before the two injections, and before and after the treatment each day. The results indicate that there was a significant reduction of both mechanical withdrawal threshold and paw withdrawal latency 24 hours following the second injection. Therefore, both mechanical and thermal hyperalgesia were significantly reversed by EA 15 Hz, EA 100 Hz and acupuncture. It can be suggested that as high and low frequency eletroacupuncture as acupuncture is effective in hiperalgesia reducing in this model of chronic muscle pain.
publishDate 2012
dc.date.issued.fl_str_mv 2012-01-25
dc.date.accessioned.fl_str_mv 2017-09-26T12:17:11Z
dc.date.available.fl_str_mv 2017-09-26T12:17:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.publisher.program.fl_str_mv Pós-Graduação em Ciências da Saúde
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