Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/3761 |
Resumo: | Repeated injections of acidic saline into the gastrocnemius muscle in rats produce a bilateral long-lasting hiperalgesia similar to fibromyalgia syndrome in humans. After the induction, it´s possible to test the efficacy of analgesic strategies such as electroacupuncture and acupuncture. This study aimed to identify the effects of acupuncture and electroacupuncture in the hyperalgesia produced by repeated intramuscular injections of acidic saline. Twenty four male Wistar rats were divided into four groups (n = 6, in each group): control, acupuncture, electroacupuncture (EA) 15 Hz and (EA) 100 Hz. Left gastrocnemius muscle from all animals was injected with 100 μL of pH 4.0 sterile saline twice five days apart. Electroacupuncture, acupuncture or control was daily administered (20 min) for 5 consecutive days under isoflurane anesthesia (2% 5%). Needles were placed in the Zuzanli (St36) and Sanyinjiao (SP6). We assessed the secondary mechanical hyperalgesia, thermal hyperalgesia and motor performance before the two injections, and before and after the treatment each day. The results indicate that there was a significant reduction of both mechanical withdrawal threshold and paw withdrawal latency 24 hours following the second injection. Therefore, both mechanical and thermal hyperalgesia were significantly reversed by EA 15 Hz, EA 100 Hz and acupuncture. It can be suggested that as high and low frequency eletroacupuncture as acupuncture is effective in hiperalgesia reducing in this model of chronic muscle pain. |
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Maciel, Leonardo Yung dos Santoshttp://lattes.cnpq.br/9819654988177433Santana, Josimari Melo dehttp://lattes.cnpq.br/34369255129515082017-09-26T12:17:11Z2017-09-26T12:17:11Z2012-01-25https://ri.ufs.br/handle/riufs/3761Repeated injections of acidic saline into the gastrocnemius muscle in rats produce a bilateral long-lasting hiperalgesia similar to fibromyalgia syndrome in humans. After the induction, it´s possible to test the efficacy of analgesic strategies such as electroacupuncture and acupuncture. This study aimed to identify the effects of acupuncture and electroacupuncture in the hyperalgesia produced by repeated intramuscular injections of acidic saline. Twenty four male Wistar rats were divided into four groups (n = 6, in each group): control, acupuncture, electroacupuncture (EA) 15 Hz and (EA) 100 Hz. Left gastrocnemius muscle from all animals was injected with 100 μL of pH 4.0 sterile saline twice five days apart. Electroacupuncture, acupuncture or control was daily administered (20 min) for 5 consecutive days under isoflurane anesthesia (2% 5%). Needles were placed in the Zuzanli (St36) and Sanyinjiao (SP6). We assessed the secondary mechanical hyperalgesia, thermal hyperalgesia and motor performance before the two injections, and before and after the treatment each day. The results indicate that there was a significant reduction of both mechanical withdrawal threshold and paw withdrawal latency 24 hours following the second injection. Therefore, both mechanical and thermal hyperalgesia were significantly reversed by EA 15 Hz, EA 100 Hz and acupuncture. It can be suggested that as high and low frequency eletroacupuncture as acupuncture is effective in hiperalgesia reducing in this model of chronic muscle pain.Repetidas injeções de salina ácida no músculo gastrocnêmio de ratos produzem uma hiperalgesia bilateral de longa duração semelhante a fibromialgia em humanos. Após esta indução, é possível analisar a eficácia de modalidades analgésicas como a acupuntura e eletroacupuntura. O objetivo deste estudo foi identificar o efeito da eletroacupuntura e acupuntura na hiperalgesia bilateral produzida por dupla injeção intramuscular de salina ácida. Foram utilizados 24 ratos Wistar machos, divididos em quatro grupos: controle, acupuntura, eletroacupuntura (EA) de 15 Hz e EA de 100 Hz. No músculo gastrocnêmio esquerdo de todos os animais, foi injetado um volume de 100 μL de solução salina estéril pH 4,0 em duas ocasiões, com intervalo de 5 dias entre elas. O tratamento com EA foi feito diariamente por 5 dias consecutivos, com duração de 20 minutos. Os animais foram anestesiados com isoflurano (2% -5%) para possibilitar a intervenção. As agulhas foram colocadas nos pontos Zuzanli (St36) e Sanyinjiao (SP6). Foram avaliados a hiperalgesia mecânica secundária, hiperalgesia térmica e desempenho motor antes das duas injeções, e antes e após o tratamento. Os resultados indicam que ocorreu uma redução significativa do limiar mecânico e da latência térmica 24 horas após a segunda injeção. Além disso, houve reversão significativa da hiperalgesia mecânica e térmica após administração de EA 15 Hz, EA 100 Hz e acupuntura. Estes dados sugerem que tanto a EA em alta ou baixa freqüência quanto a acupuntura são eficazes na redução da hiperalgesia neste modelo de dor muscular crônica.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de SergipePós-Graduação em Ciências da SaúdeUFSBRHiperalgesiaDorEletroacupunturaFibromialgiaAnalgesiaRatosHyperalgesiaPainFibromyalgiaElectroacupunctureAnalgesiaRatCNPQ::CIENCIAS DA SAUDEEletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônicaELECTROACUPUNCTURE AND ACUPUNCTURE REDUCES HYPERALGESIA PRODUCED BY REPEATED INTRAMUSCULAR INJECTIONS OF ACIDIC SALINE IN RATS.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSTEXTLEONARDO_YUNG_SANTOS_MACIEL.pdf.txtLEONARDO_YUNG_SANTOS_MACIEL.pdf.txtExtracted texttext/plain85938https://ri.ufs.br/jspui/bitstream/riufs/3761/2/LEONARDO_YUNG_SANTOS_MACIEL.pdf.txt9d78910055bb06286206f2f033701c74MD52THUMBNAILLEONARDO_YUNG_SANTOS_MACIEL.pdf.jpgLEONARDO_YUNG_SANTOS_MACIEL.pdf.jpgGenerated Thumbnailimage/jpeg1499https://ri.ufs.br/jspui/bitstream/riufs/3761/3/LEONARDO_YUNG_SANTOS_MACIEL.pdf.jpg8a9bff025522de1324e987cdbe92b65aMD53ORIGINALLEONARDO_YUNG_SANTOS_MACIEL.pdfapplication/pdf931646https://ri.ufs.br/jspui/bitstream/riufs/3761/1/LEONARDO_YUNG_SANTOS_MACIEL.pdf72e7a81d8a73c69afb97d4b164a218e1MD51riufs/37612017-11-28 16:38:00.545oai:ufs.br:riufs/3761Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-28T19:38Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.por.fl_str_mv |
Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica |
dc.title.alternative.eng.fl_str_mv |
ELECTROACUPUNCTURE AND ACUPUNCTURE REDUCES HYPERALGESIA PRODUCED BY REPEATED INTRAMUSCULAR INJECTIONS OF ACIDIC SALINE IN RATS. |
title |
Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica |
spellingShingle |
Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica Maciel, Leonardo Yung dos Santos Hiperalgesia Dor Eletroacupuntura Fibromialgia Analgesia Ratos Hyperalgesia Pain Fibromyalgia Electroacupuncture Analgesia Rat CNPQ::CIENCIAS DA SAUDE |
title_short |
Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica |
title_full |
Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica |
title_fullStr |
Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica |
title_full_unstemmed |
Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica |
title_sort |
Eletroacupuntura reduz hiperalgesia em modelo animal de hiperalgesia induzida por dupla injeção de salina hipertônica |
author |
Maciel, Leonardo Yung dos Santos |
author_facet |
Maciel, Leonardo Yung dos Santos |
author_role |
author |
dc.contributor.author.fl_str_mv |
Maciel, Leonardo Yung dos Santos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9819654988177433 |
dc.contributor.advisor1.fl_str_mv |
Santana, Josimari Melo de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3436925512951508 |
contributor_str_mv |
Santana, Josimari Melo de |
dc.subject.por.fl_str_mv |
Hiperalgesia Dor Eletroacupuntura Fibromialgia Analgesia Ratos |
topic |
Hiperalgesia Dor Eletroacupuntura Fibromialgia Analgesia Ratos Hyperalgesia Pain Fibromyalgia Electroacupuncture Analgesia Rat CNPQ::CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
Hyperalgesia Pain Fibromyalgia Electroacupuncture Analgesia Rat |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE |
description |
Repeated injections of acidic saline into the gastrocnemius muscle in rats produce a bilateral long-lasting hiperalgesia similar to fibromyalgia syndrome in humans. After the induction, it´s possible to test the efficacy of analgesic strategies such as electroacupuncture and acupuncture. This study aimed to identify the effects of acupuncture and electroacupuncture in the hyperalgesia produced by repeated intramuscular injections of acidic saline. Twenty four male Wistar rats were divided into four groups (n = 6, in each group): control, acupuncture, electroacupuncture (EA) 15 Hz and (EA) 100 Hz. Left gastrocnemius muscle from all animals was injected with 100 μL of pH 4.0 sterile saline twice five days apart. Electroacupuncture, acupuncture or control was daily administered (20 min) for 5 consecutive days under isoflurane anesthesia (2% 5%). Needles were placed in the Zuzanli (St36) and Sanyinjiao (SP6). We assessed the secondary mechanical hyperalgesia, thermal hyperalgesia and motor performance before the two injections, and before and after the treatment each day. The results indicate that there was a significant reduction of both mechanical withdrawal threshold and paw withdrawal latency 24 hours following the second injection. Therefore, both mechanical and thermal hyperalgesia were significantly reversed by EA 15 Hz, EA 100 Hz and acupuncture. It can be suggested that as high and low frequency eletroacupuncture as acupuncture is effective in hiperalgesia reducing in this model of chronic muscle pain. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-01-25 |
dc.date.accessioned.fl_str_mv |
2017-09-26T12:17:11Z |
dc.date.available.fl_str_mv |
2017-09-26T12:17:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
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https://ri.ufs.br/handle/riufs/3761 |
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https://ri.ufs.br/handle/riufs/3761 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Sergipe |
dc.publisher.program.fl_str_mv |
Pós-Graduação em Ciências da Saúde |
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UFS |
dc.publisher.country.fl_str_mv |
BR |
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Universidade Federal de Sergipe |
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UFS |
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