Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein

Detalhes bibliográficos
Autor(a) principal: Cândido, Alexandre Luna
Data de Publicação: 2003
Outros Autores: Resende, Maurício, Bessa, Luciana Regina Guimarães, Leite, Rômulo Cerqueira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/861
Resumo: A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a. _________________________________________________________________________________________ ABSTRACT: Uma vacina geneticamente preparada com o gene da glicoproteína D do herpesvirus bovino-1.2a (BHV-1.2a) controlado com o promotor/potenciador imediatamente precoce de citomegalovírus foi administrada no pescoço de ovinos. Todos os animais produziram anticorpos séricos que reconheceram, em ELISA, o antígeno homólogo (BHV-1.2a, amostra BH-83) e também mostraram reatividade cruzada contra um antígeno heterólogo (BHV-5 amostra EVI-190). Foram aplicadas três inoculações, mas não houve melhora de resposta com a terceira inoculação. Resposta específica contra BHV-1.2a foi detectada pelo menos até 12 meses após a primeira inoculação, entretanto, a resposta sorológica contra o BHV-5 foi de menor intensidade e duração do que aquela contra o BHV-1.2a.
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spelling Cândido, Alexandre LunaResende, MaurícioBessa, Luciana Regina GuimarãesLeite, Rômulo Cerqueira2014-02-03T17:35:30Z2014-02-03T17:35:30Z2003-06CANDIDO, A. L. et al. Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 55, n. 3, jun. 2003. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300002&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.0102-0935https://ri.ufs.br/handle/riufs/861Creative Commons Attribution LicenseA genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a. _________________________________________________________________________________________ ABSTRACT: Uma vacina geneticamente preparada com o gene da glicoproteína D do herpesvirus bovino-1.2a (BHV-1.2a) controlado com o promotor/potenciador imediatamente precoce de citomegalovírus foi administrada no pescoço de ovinos. Todos os animais produziram anticorpos séricos que reconheceram, em ELISA, o antígeno homólogo (BHV-1.2a, amostra BH-83) e também mostraram reatividade cruzada contra um antígeno heterólogo (BHV-5 amostra EVI-190). Foram aplicadas três inoculações, mas não houve melhora de resposta com a terceira inoculação. Resposta específica contra BHV-1.2a foi detectada pelo menos até 12 meses após a primeira inoculação, entretanto, a resposta sorológica contra o BHV-5 foi de menor intensidade e duração do que aquela contra o BHV-1.2a.Escola de Veterinária da UFMGOvinosVacinas veterináriasTeste ELISAHerpesvirus bovinoVacina de DNASerological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoproteinResposta sorológica de ovinos inoculados com plasmídeos codificando a glicoproteína D do herpesvírus bovino 1 (BHV-1)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILSerologicalResponsesSheep.pdf.jpgSerologicalResponsesSheep.pdf.jpgGenerated Thumbnailimage/jpeg1693https://ri.ufs.br/jspui/bitstream/riufs/861/4/SerologicalResponsesSheep.pdf.jpgdbd9e2c708f94c51d0f9bd9313a444b0MD54ORIGINALSerologicalResponsesSheep.pdfSerologicalResponsesSheep.pdfapplication/pdf115176https://ri.ufs.br/jspui/bitstream/riufs/861/1/SerologicalResponsesSheep.pdf0092c79d329573666dfd3421fb276bd0MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/861/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTSerologicalResponsesSheep.pdf.txtSerologicalResponsesSheep.pdf.txtExtracted texttext/plain24161https://ri.ufs.br/jspui/bitstream/riufs/861/3/SerologicalResponsesSheep.pdf.txt82d60c9b60636a74befb12523ce344e3MD53riufs/8612014-02-04 02:00:26.705oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2014-02-04T05:00:26Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
dc.title.alternative.pt_BR.fl_str_mv Resposta sorológica de ovinos inoculados com plasmídeos codificando a glicoproteína D do herpesvírus bovino 1 (BHV-1)
title Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
spellingShingle Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
Cândido, Alexandre Luna
Ovinos
Vacinas veterinárias
Teste ELISA
Herpesvirus bovino
Vacina de DNA
title_short Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
title_full Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
title_fullStr Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
title_full_unstemmed Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
title_sort Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
author Cândido, Alexandre Luna
author_facet Cândido, Alexandre Luna
Resende, Maurício
Bessa, Luciana Regina Guimarães
Leite, Rômulo Cerqueira
author_role author
author2 Resende, Maurício
Bessa, Luciana Regina Guimarães
Leite, Rômulo Cerqueira
author2_role author
author
author
dc.contributor.author.fl_str_mv Cândido, Alexandre Luna
Resende, Maurício
Bessa, Luciana Regina Guimarães
Leite, Rômulo Cerqueira
dc.subject.por.fl_str_mv Ovinos
Vacinas veterinárias
Teste ELISA
Herpesvirus bovino
Vacina de DNA
topic Ovinos
Vacinas veterinárias
Teste ELISA
Herpesvirus bovino
Vacina de DNA
description A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a. _________________________________________________________________________________________ ABSTRACT: Uma vacina geneticamente preparada com o gene da glicoproteína D do herpesvirus bovino-1.2a (BHV-1.2a) controlado com o promotor/potenciador imediatamente precoce de citomegalovírus foi administrada no pescoço de ovinos. Todos os animais produziram anticorpos séricos que reconheceram, em ELISA, o antígeno homólogo (BHV-1.2a, amostra BH-83) e também mostraram reatividade cruzada contra um antígeno heterólogo (BHV-5 amostra EVI-190). Foram aplicadas três inoculações, mas não houve melhora de resposta com a terceira inoculação. Resposta específica contra BHV-1.2a foi detectada pelo menos até 12 meses após a primeira inoculação, entretanto, a resposta sorológica contra o BHV-5 foi de menor intensidade e duração do que aquela contra o BHV-1.2a.
publishDate 2003
dc.date.issued.fl_str_mv 2003-06
dc.date.accessioned.fl_str_mv 2014-02-03T17:35:30Z
dc.date.available.fl_str_mv 2014-02-03T17:35:30Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CANDIDO, A. L. et al. Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 55, n. 3, jun. 2003. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300002&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/handle/riufs/861
dc.identifier.issn.none.fl_str_mv 0102-0935
dc.identifier.license.pt_BR.fl_str_mv Creative Commons Attribution License
identifier_str_mv CANDIDO, A. L. et al. Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 55, n. 3, jun. 2003. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300002&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.
0102-0935
Creative Commons Attribution License
url https://ri.ufs.br/handle/riufs/861
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Escola de Veterinária da UFMG
publisher.none.fl_str_mv Escola de Veterinária da UFMG
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
instname:Universidade Federal de Sergipe (UFS)
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instname_str Universidade Federal de Sergipe (UFS)
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institution UFS
reponame_str Repositório Institucional da UFS
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