Prevalência do papilomavírus humano (HPV) em lesões malignas da cavidade oral e sua associação com fatores de risco para o câncer oral

• Autor(a) principal: Araujo, Bruno Eduardo Silva de
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: https://ri.ufs.br/handle/riufs/3853
• topic: HPV; Câncer de boca; PCR; HPV; Oral cancer; PCR; CNPQ::CIENCIAS DA SAUDE

Oral cancer is one of the most common cancers worldwide, in Brazil is estimated for the years 2012 and 2013 the rate of 9,990 new cases. The main risk factors for oral cancer are smoking, alcohol consumption and infection by human papillomavirus (HPV). Due to the impact of HPV infection in sexually active population, and the fact that the infection is an important risk factor for development of oral cancer, the analysis of the presence of the virus in patients with suspicious lesions (oral cancer) proves to be important for understanding infection and innovation diagnosis. This study will emphasize the importance of including in patient diagnosis the analysis of HPV infection, as this would influence prognosis and treatment of disease. Furthermore, this work may contribute to other studies which aim to determine the incidence of HPV in the oral mucosa. The present study aimed to analyze the possible relationship of the virus in the development of malignant oral lesions. The samples of oral biopsy were taken from 25 patients treated at the Clinic of Stomatology of the University Hospital of UFSC in the period from 2006 to 2011. The study was the type retrospective, cross-sectional descriptive with qualitative analysis of material extraction colected. After the extraction by salting-out method, the samples were analyzed by PCR. PCO3/PCO4 primer were used for beta globin gene, MY09/MY11 for HPV detection and GP5 + / GP6 + for nested PCR. The association between patient age and the presence of HPV was analyzed using the Student t test for independent samples. Variables such as smoking, drinking and sex were analyzed using the Fisher exact test. The association between HPV and oral cancer was analyzed using the chi-square test. About 40% of the samples were infected with HPV. The analysis of factors related to HPV infection in neoplastic lesions showed that patients infected with HPV are significantly older than patients without infection. The other variables showed no significant difference