Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)

Detalhes bibliográficos
Autor(a) principal: Santos, Mário Ferreira Conceição
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/6151
Resumo: This work describes the isolation of endophytic fungi from plant species Hancornia speciosa and led to the isolation of 14 pure strains. Of which 4 were selected for the chemical study. The chemical study allowed identied 12 substances isolated from fungi Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., grown in different culture medium. The crude extracts of fungus Phomopsis sp., grown in PDB medium led to isolation of five compounds, 5- methylmelein, nectriapyrone, succnic acid, 5-hydroxylmethylmelein and a diketopiperazine. Already when the same fungus was grown in ME medium, led to isolation of two compounds, tyrosol and tryptofol. The crude extract of Guignardia sp grown in ME medium led to isolation of compound salicylic acid, which has a plant for ecological importance.The crude extract of Aspergillus niger, grown in PDB medium led to isolation of compounds pyrophen and nigragillin. Already when the same fungus was grown in ME medium, led to isolation of compound itaconic acid. And of crude extract of Aspergillus sp., grown in ME medium was identification of their major compound kojic acid, which was quantified by HPLC in the crude extract (0.7 mg/g of crude extract). O crude extract was tested for its antioxidant activity with DPPH, NO. and H2O2, and exhibited significantly potent inhibition of NO (IC50 de 150 μg/mL) production, showed taht this fungi it owns big biotchenology potential. The structures of these substances were established by spectroscopic methods, including the application of bidimensional NMR techniques, mass spectrum and comparison with published data.
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spelling Santos, Mário Ferreira Conceiçãohttp://lattes.cnpq.br/9385808202043341Silva, Geraldo Humbertohttp://lattes.cnpq.br/78154898247541852017-09-27T13:58:07Z2017-09-27T13:58:07Z2011-02-16https://ri.ufs.br/handle/riufs/6151This work describes the isolation of endophytic fungi from plant species Hancornia speciosa and led to the isolation of 14 pure strains. Of which 4 were selected for the chemical study. The chemical study allowed identied 12 substances isolated from fungi Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., grown in different culture medium. The crude extracts of fungus Phomopsis sp., grown in PDB medium led to isolation of five compounds, 5- methylmelein, nectriapyrone, succnic acid, 5-hydroxylmethylmelein and a diketopiperazine. Already when the same fungus was grown in ME medium, led to isolation of two compounds, tyrosol and tryptofol. The crude extract of Guignardia sp grown in ME medium led to isolation of compound salicylic acid, which has a plant for ecological importance.The crude extract of Aspergillus niger, grown in PDB medium led to isolation of compounds pyrophen and nigragillin. Already when the same fungus was grown in ME medium, led to isolation of compound itaconic acid. And of crude extract of Aspergillus sp., grown in ME medium was identification of their major compound kojic acid, which was quantified by HPLC in the crude extract (0.7 mg/g of crude extract). O crude extract was tested for its antioxidant activity with DPPH, NO. and H2O2, and exhibited significantly potent inhibition of NO (IC50 de 150 μg/mL) production, showed taht this fungi it owns big biotchenology potential. The structures of these substances were established by spectroscopic methods, including the application of bidimensional NMR techniques, mass spectrum and comparison with published data.Este trabalho descreve o isolamento de fungos endofíticos da espécie vegetal Hancornia speciosa, o qual conduziu a 14 linhagens puras, dos quais quatro foram selecionados para o estudo químico. O estudo químico possibilitou foi identicar 12 substâncias, isoladas dos fungos Phomopsis sp., Guignardia sp., Aspergillus níger e Aspergillus sp., cultivados em diferentes meios de culturas. O extrato bruto do fungo Phomopsis sp cultivado no meio liquido PBD levou ao isolamento de cinco compostos, 5- metilmeleína, nectapirona, ácido succínico, 5-hidroxilmetilmeleína e uma dicetopiperazina. Já o mesmo fungo quando cultivado no meio líquido-ME, levou ao isolamento de dois compostos, tirosol e triptofol. O estudo do extrato bruto de Guignardia sp., cultivado no meio liquido ME conduziu ao isolamento do composto ácido salicílico, o qual tem um importancia ecológica para planta. O extrato bruto do fungo Aspergillus niger cultivado no meio liquido PBD levou ao isolamento dos compostos pirofen e nigragillin. Já o mesmo fungo quando cultivado em extrato de malte, levou ao isolamento do ácido itacônico. E do extrato bruto do fungo Aspergillus sp., cultivado no meio liquido PBD foi identificada a substância majoritária ácido kójico, a qual foi quantificada por CLAE no extrato bruto (0,7g/ g de extrato bruto). O extrato desse fungo foi testado quanto a sua atividade antioxidante com DPPH, NO. , e H2O2, e mostrou uma forte atividade de inibição da produção de NO (IC50 de 150 μg/mL), indicando que este fungo possui grande potencial biotecnológico. As estruturas das substâncias foram determinadas por métodos espectrométricos, incluindo RMN bi-dimensional, espectros de massas e comparação com a literatura.application/pdfporUniversidade Federal de SergipePós-Graduação em QuímicaUFSBRFungos endofíticosHamcornia speciosaMetabolitos secundários bioativosEndophytic fungiHancornia speciosaSecundary metabolitesCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAEstudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)CHEMICAL INVESTIGATION OF THE ENDOPHYTIC FUNGUS PHOMOPSIS SP., GUIGNARD SP., ASPERGILLUS NIGER AND ASPERGILLUS SP., PLANT SPECIES ASSOCIATED WITH HANCORNIA SPECIOSA (APOCINACEAE).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSORIGINALMARIO_FERREIRA_CONCEICAO_SANTOS.pdfapplication/pdf5246594https://ri.ufs.br/jspui/bitstream/riufs/6151/1/MARIO_FERREIRA_CONCEICAO_SANTOS.pdfb249e24392a5ea6e5396da5673e3e8b6MD51TEXTMARIO_FERREIRA_CONCEICAO_SANTOS.pdf.txtMARIO_FERREIRA_CONCEICAO_SANTOS.pdf.txtExtracted texttext/plain219285https://ri.ufs.br/jspui/bitstream/riufs/6151/2/MARIO_FERREIRA_CONCEICAO_SANTOS.pdf.txt31171f7cb7d1fc0750b6dc974e1ca874MD52THUMBNAILMARIO_FERREIRA_CONCEICAO_SANTOS.pdf.jpgMARIO_FERREIRA_CONCEICAO_SANTOS.pdf.jpgGenerated Thumbnailimage/jpeg1308https://ri.ufs.br/jspui/bitstream/riufs/6151/3/MARIO_FERREIRA_CONCEICAO_SANTOS.pdf.jpg36574ae753242c6165a78a2437be9423MD53riufs/61512018-01-16 19:50:13.746oai:ufs.br:riufs/6151Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-01-16T22:50:13Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.por.fl_str_mv Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)
dc.title.alternative.eng.fl_str_mv CHEMICAL INVESTIGATION OF THE ENDOPHYTIC FUNGUS PHOMOPSIS SP., GUIGNARD SP., ASPERGILLUS NIGER AND ASPERGILLUS SP., PLANT SPECIES ASSOCIATED WITH HANCORNIA SPECIOSA (APOCINACEAE).
title Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)
spellingShingle Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)
Santos, Mário Ferreira Conceição
Fungos endofíticos
Hamcornia speciosa
Metabolitos secundários bioativos
Endophytic fungi
Hancornia speciosa
Secundary metabolites
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)
title_full Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)
title_fullStr Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)
title_full_unstemmed Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)
title_sort Estudo químico dos fungos endofíticos Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., associados à espécie vegetal Hancornia speciosa (Apocinaceae)
author Santos, Mário Ferreira Conceição
author_facet Santos, Mário Ferreira Conceição
author_role author
dc.contributor.author.fl_str_mv Santos, Mário Ferreira Conceição
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9385808202043341
dc.contributor.advisor1.fl_str_mv Silva, Geraldo Humberto
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7815489824754185
contributor_str_mv Silva, Geraldo Humberto
dc.subject.por.fl_str_mv Fungos endofíticos
Hamcornia speciosa
Metabolitos secundários bioativos
topic Fungos endofíticos
Hamcornia speciosa
Metabolitos secundários bioativos
Endophytic fungi
Hancornia speciosa
Secundary metabolites
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Endophytic fungi
Hancornia speciosa
Secundary metabolites
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
description This work describes the isolation of endophytic fungi from plant species Hancornia speciosa and led to the isolation of 14 pure strains. Of which 4 were selected for the chemical study. The chemical study allowed identied 12 substances isolated from fungi Phomopsis sp., Guignardia sp., Aspergillus niger e Aspergillus sp., grown in different culture medium. The crude extracts of fungus Phomopsis sp., grown in PDB medium led to isolation of five compounds, 5- methylmelein, nectriapyrone, succnic acid, 5-hydroxylmethylmelein and a diketopiperazine. Already when the same fungus was grown in ME medium, led to isolation of two compounds, tyrosol and tryptofol. The crude extract of Guignardia sp grown in ME medium led to isolation of compound salicylic acid, which has a plant for ecological importance.The crude extract of Aspergillus niger, grown in PDB medium led to isolation of compounds pyrophen and nigragillin. Already when the same fungus was grown in ME medium, led to isolation of compound itaconic acid. And of crude extract of Aspergillus sp., grown in ME medium was identification of their major compound kojic acid, which was quantified by HPLC in the crude extract (0.7 mg/g of crude extract). O crude extract was tested for its antioxidant activity with DPPH, NO. and H2O2, and exhibited significantly potent inhibition of NO (IC50 de 150 μg/mL) production, showed taht this fungi it owns big biotchenology potential. The structures of these substances were established by spectroscopic methods, including the application of bidimensional NMR techniques, mass spectrum and comparison with published data.
publishDate 2011
dc.date.issued.fl_str_mv 2011-02-16
dc.date.accessioned.fl_str_mv 2017-09-27T13:58:07Z
dc.date.available.fl_str_mv 2017-09-27T13:58:07Z
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dc.publisher.program.fl_str_mv Pós-Graduação em Química
dc.publisher.initials.fl_str_mv UFS
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal de Sergipe
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