Atividade biológica de extratos orgânicos obtidos a partir da fermentação de fungos endofíticos isolados de humirianthera ampla

• Autor(a) principal: Alves, Lucas Vinícius de Jesus
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/12353
• topic: Agentes antineoplásicos; Fermentação; Fungos; Atividade citotóxica; Atividade antibacteriana; Métodos de extração; Hawksworthiomyces sp.; Arthrographis sp.; Cytotoxic activity; Antibacterial activity; Extraction methods

Endophytic fungi are potential producers of bioactive substances that perform various biological functions. This work was an investigation of the biological activities of the organic extracts of endophytic fungi Humirianthera ampla. n the identification the genetic materials were extracted, amplified, sequenced and analyzed by a phylogenetic study. UFCM 0533 fungus was grown in different culture media (potato dextrose - BD, sabouraud dextrose - SBD, meat extract and peptone - MEP and rice) for 22 days. After this period were extracted by different methods of extraction: maceration, ultrasound and ultrasound combined with maceration in order to determine what provided greater biological yield. The chemical profile of the extracts was evaluated by analytical thin layer chromatography (ADCC). In the biological activities the extracts of two fungi were used, and extracts of UFCM 0550 were obtained in previous studies by our research group. The extracts were tested for antibacterial activity against Escherichia coli and Staphylococcus aureus and cytotoxic activity against four tumor cell lines - HCT-116 (Colorretal), SF295 (Glioblastoma), PC3 (Prostate) and HL 60 (leukemic). In the phylogenetic analysis, isolates UFCM 0533 and UFCM 0550 were respectively designated as Hawksworthiomyces sp. and Arthrographis sp. The analyzes revealed significant differences between the extracts and that the best condition in terms of higher biological yield was the extracts from the BD medium and extracted by maceration. The ADCC of the extracts of Hawksworthiomyces sp. revealed a change in the production of compounds in extracts of different growth media and extraction methods. In the antibacterial test of the extracts of both fungi, only rice extracts from the Hawksworthiomyces sp fungus inhibited Staphylococcus aureus. In the cytotoxicity test of Hawksworthiomyces sp fungi extracts the most promising was obtained from growth in BD and extracted by maceration that inhibited two tumor cell lines (leukemic and colorectal).