Arcabouços 3D (Scaffolds) à base de poli (hidroxibutirato), quitosana e fibroína da seda para engenharia tecidual

• Autor(a) principal: Macedo, Maria Erisfagna Ribeiro de
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: https://ri.ufs.br/handle/riufs/3543
• topic: Engenharia de materiais; Quitosana; Ossos; Tecidos; Seda; Butiratos; Polímeros; Arcabouços 3D; Poli(hidroxibutirato); Polihidroxibutirato; Quitosana; Fibroína da seda; Testes in vitro; 3D scaffolds; Poly(hydroxybutyrate); Polyhydroxybutyrate; Chitosan; Silk fibroin; In vitro tests; ENGENHARIAS::ENGENHARIA DE MATERIAIS E METALURGICA

Materials based on polyhydroxybutyrate (PHB), chitosan (CHI) and fibroin (SF) are biocompatible and attractive for applications in bone tissue engineering. In this work, 3D scaffolds of PHB/CHI and PHB/CHI/SF in different proportions was prepared, characterized and evaluated the in vitro behavior: group I: PHB/CHI (50:50 wt.%), group II: PHB/CHI/SF (50:45:5 wt.%) and group III: PHB/CHI/SF (50:35:15 wt.%). The scaffolds were produced by the lyophilization method of the components mixtures. The physical-chemical characterization of the scaffolds was performed by X-ray diffraction, infrared spectroscopy, scanning electron microscopy and thermogravimetric analysis. A highly porous nature was revealed by SEM analysis of the scaffolds. The FTIR analysis revealed that the constituents used in the preparation of the scaffolds interacted chemically with each other. Thermal analysis showed that fibroin increases the thermal stability of the scaffolds. The viability and cell proliferation were assessed by the MTT method and the cytotoxicity results showed that all scaffolds are non- cytotoxic. In addition, the scaffolds stimulated cell proliferation and are promising for tissue engineering applications.