Desenvolvimento de método para determinação de resíduos de agrotóxicos na fruta exótica graviola (Anonna municata L.)

• Autor(a) principal: Santos, Luis Fabrício Santana
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: https://ri.ufs.br/handle/riufs/6093
• topic: Química analítica; Produtos químicos agrícolas; Graviola; Cromatografia a líquido; Agrotóxicos; Annona muricata L.; MSPD; HPLC; UV-Vis/DAD; MOF; Pesticides; Soursop; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA

Brazil is a great producer and consumer of fruits in the world. The soursop (Annona muricata L.) is a fruit with special flavor and aroma and with growing demand for fresh or processed consumption, such as juices, jams, fruit jellies and ice cream. However, the culture of soursop is subjected to the action of different pests and diseases and the application of pesticides has been one of the main ways of control. The present work aims at developing a rapid and low-cost methodology based on matrix solid phase dispersion and high-performance liquid chromatography with diode-array detection (HPLC UV-Vis/DAD) for the determination of pesticide residues of Bifenthrin, Lufenuron, Teflubenzuron, Thiacloprid, Thiophanate-methyl and Thiamethoxam, in the exotic fruit soursop. Some tests conducted with the standards of pesticides allowed the adjustment of the chromatographic conditions for simultaneous analysis of analytes. The best extraction conditions were obtained using 1.0 g of Florisil to 0.5 g of fresh soursop and Elution with 5 mL of Acetonitrile. The miniaturization of the extraction procedure was shown to be efficient in the extraction of pesticides. Analytical validation was performed from the parameters: linearity and sensitivity, with coefficients of determination over 0.9995 and sensitive concentration range of 0.01 -10 g mL-1; selectivity, from the matrix effect studies; accuracy and precision, with recovery values between -118 79% and RSD in the range of 1- 15% for concentration levels 0.1, 0.25 and 0.5 g g-1 (n = 5); and limits of detection and quantification in the intervals of 0.01-0.25 g g-1 and 0.1-0.5 g g- 1, respectively. Metal Organic Frameworks (MOF) [(Nd0, 9Eu0.1)2(DPA)3(H2O)3] was tested as sorbent for the MSPD extraction process and presented satisfactory recovery results (78-87%) compared to those achieved with Florisil.