Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe

Detalhes bibliográficos
Autor(a) principal: Dias Filho, Vinicius Augusto
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/6378
Resumo: Cryopreservation of fish semen is a key to optimization of reproductive management, the formation of germplasm bank and the development of genetic improvement programs. The success of this technique depends on the management and balance of several factors such as the collection, dilution, packaging, freezing and thawing. Tambaqui semen cryopreservation protocols have been developed using 0.5 mL straws on packaging. However, the high volumes of semen produced and the elevated taxes of fecundity are presented by this specie, it is necessary to use larger volume storage containers for application to large-scale production. The objective of this study was to establish a tambaqui semen cryopreservation protocol in large straw 4,0mL, from the definition of cryosolution, equilibration time and the best ratio between temperature and time semen thawing. The semen collected was diluted, packaged in large straws, frozen in liquid nitrogen which was conditioned in dry-shipper and stored in cryogenic cylinder at -196 °C. In the first experiment, different cryosolutions (M1 - 5% 5% methylglycol; M2 - 5% 5% methylglycol+ 5% egg yolk; M3 - 10% 5% methylglycol; M4 - 10% 5% methylglycol + 5% egg yolk; M5 - 15% 5% methylglycol; M6 - 15% de 5% methylglycol + 5% egg yolk) and three equilibration times (4, 20 and 40 minutes) were tested. In the second experiment, different relation between temperature and time thawing on sperm quality (30ºC for 50s - T1; 30ºC for 80s - T2; 60ºC for 25s - T3 e 60ºC for 40s - T4) were evaluated. In conclusion, this study showed that the ideal protocol for cryopreservation of tambaqui semen in large straw 4,0mL is made by diluting semen at a ratio of 1: 9 (v: v) in a medium composed of 5% methylglycol and 5% egg yolk. Both of them must have remained in contact with each other for 4 minutes until both were subjected to freezing in dry-shipper. The thawing of samples required to be performed in a water bath at 60 ° C for 25s.
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spelling Dias Filho, Vinicius Augustohttp://lattes.cnpq.br/2964724004718343Maria, Alexandre Niziohttp://lattes.cnpq.br/71389247158762352017-09-27T18:04:11Z2017-09-27T18:04:11Z2015-02-25DIAS FILHO, Vinicius Augusto. Cryopreservation of tambaqui (Colossoma macropomum) semen in large straws. 2015. 43 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Sergipe, São Cristóvão, 2015.https://ri.ufs.br/handle/riufs/6378Cryopreservation of fish semen is a key to optimization of reproductive management, the formation of germplasm bank and the development of genetic improvement programs. The success of this technique depends on the management and balance of several factors such as the collection, dilution, packaging, freezing and thawing. Tambaqui semen cryopreservation protocols have been developed using 0.5 mL straws on packaging. However, the high volumes of semen produced and the elevated taxes of fecundity are presented by this specie, it is necessary to use larger volume storage containers for application to large-scale production. The objective of this study was to establish a tambaqui semen cryopreservation protocol in large straw 4,0mL, from the definition of cryosolution, equilibration time and the best ratio between temperature and time semen thawing. The semen collected was diluted, packaged in large straws, frozen in liquid nitrogen which was conditioned in dry-shipper and stored in cryogenic cylinder at -196 °C. In the first experiment, different cryosolutions (M1 - 5% 5% methylglycol; M2 - 5% 5% methylglycol+ 5% egg yolk; M3 - 10% 5% methylglycol; M4 - 10% 5% methylglycol + 5% egg yolk; M5 - 15% 5% methylglycol; M6 - 15% de 5% methylglycol + 5% egg yolk) and three equilibration times (4, 20 and 40 minutes) were tested. In the second experiment, different relation between temperature and time thawing on sperm quality (30ºC for 50s - T1; 30ºC for 80s - T2; 60ºC for 25s - T3 e 60ºC for 40s - T4) were evaluated. In conclusion, this study showed that the ideal protocol for cryopreservation of tambaqui semen in large straw 4,0mL is made by diluting semen at a ratio of 1: 9 (v: v) in a medium composed of 5% methylglycol and 5% egg yolk. Both of them must have remained in contact with each other for 4 minutes until both were subjected to freezing in dry-shipper. The thawing of samples required to be performed in a water bath at 60 ° C for 25s.A criopreservação de sêmen de peixes é uma ferramenta para a otimização do manejo reprodutivo, a formação de bancos de germoplasma e o desenvolvimento de programas de melhoramento genético. O sucesso dessa técnica depende do domínio e o equilíbrio de vários fatores que envolvem os processos de coleta, diluição, envase, congelamento e descongelamento. Protocolos de criopreservação sêmen de tambaqui já foram desenvolvidos utilizando palhetas de 0,5mL no envase, no entanto, o elevado volume de sêmen produzido e alta fecundidade da espécie sugere o armazenamento em recipientes de maior volume para a aplicação na produção em larga escala. Com isso, o objetivo do presente estudo foi estabelecer um protocolo de criopreservação seminal do tambaqui em macropalhetas de 4,0mL, a partir da definição de um meio diluidor, tempo de equilíbrio e a melhor relação entre temperatura e o tempo de descongelamento do sêmen. O sêmen coletado foi diluído, envasado em macropalhetas, congelado em vapor de nitrogênio líquido no botijão dry-shipper e armazenadas em botijão criogênico a -196°C. No primeiro experimento, foram testadas diferentes composições do meio diluidor (M1 - 5% de metilglicol; M2 - 5% de metilglicol + 5% de gema de ovo; M3 - 10% de metilglicol; M4 - 10% de metilglicol + 5% de gema de ovo; M5 - 15% de metilglicol; M6 - 15% de metilglicol + 5% de gema de ovo) e três tempos de equilíbrio (4, 20 e 40 minutos). No segundo experimento, foram avaliadas diferentes velocidades de descongelamento do sêmen em banho-maria, sobre a qualidade espermática (30ºC por 50s - T1; 30ºC por 80s - T2; 60ºC por 25s - T3 e 60ºC por 40s - T4). Ao final do estudo concluiu-se, que o protocolo ideal para a criopreservação do sêmen do tambaqui em macropalhetas de 4,0mL consiste na diluição do sêmen na proporção de 1:9 (v:v) em um meio composto por 5% de metilglicol e 5% de gema de ovo, os quais devem permanecer em contato por 4 minutos até que sejam submetidos ao processo de congelamento em botijão dry-shipper. O descongelamento das amostras deve ser realizado em banho-maria a 60°C por 25s.application/pdfporUniversidade Federal de SergipePós-Graduação em ZootecniaUFSBRCinética espermáticaCrioprotetorGema de ovoMetilglicolPeixeZootecniaTambaquiCriopreservação de orgãos, tecidos, etcReprodução de peixesReprodução animalCrioprotectorEgg yolkFishMethylglycolSperm kineticsCNPQ::CIENCIAS AGRARIAS::ZOOTECNIACriopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas SergipeCryopreservation of tambaqui (Colossoma macropomum) semen in large strawsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSORIGINALVINICIUS_AUGUSTO_DIAS_FILHO.pdfapplication/pdf697052https://ri.ufs.br/jspui/bitstream/riufs/6378/1/VINICIUS_AUGUSTO_DIAS_FILHO.pdf0236da67472c289be0a94a7ae7f05331MD51TEXTVINICIUS_AUGUSTO_DIAS_FILHO.pdf.txtVINICIUS_AUGUSTO_DIAS_FILHO.pdf.txtExtracted texttext/plain92103https://ri.ufs.br/jspui/bitstream/riufs/6378/2/VINICIUS_AUGUSTO_DIAS_FILHO.pdf.txt98e79f3e4077171155ebcadbe11f08acMD52THUMBNAILVINICIUS_AUGUSTO_DIAS_FILHO.pdf.jpgVINICIUS_AUGUSTO_DIAS_FILHO.pdf.jpgGenerated Thumbnailimage/jpeg1222https://ri.ufs.br/jspui/bitstream/riufs/6378/3/VINICIUS_AUGUSTO_DIAS_FILHO.pdf.jpg5daf46ec287fbe3b4dec3a2b70f7f8fdMD53riufs/63782018-01-16 20:51:40.587oai:ufs.br:riufs/6378Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-01-16T23:51:40Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.por.fl_str_mv Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe
dc.title.alternative.eng.fl_str_mv Cryopreservation of tambaqui (Colossoma macropomum) semen in large straws
title Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe
spellingShingle Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe
Dias Filho, Vinicius Augusto
Cinética espermática
Crioprotetor
Gema de ovo
Metilglicol
Peixe
Zootecnia
Tambaqui
Criopreservação de orgãos, tecidos, etc
Reprodução de peixes
Reprodução animal
Crioprotector
Egg yolk
Fish
Methylglycol
Sperm kinetics
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
title_short Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe
title_full Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe
title_fullStr Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe
title_full_unstemmed Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe
title_sort Criopreservação de sêmen de tambaqui Colossoma macropomum em macropalhetas Sergipe
author Dias Filho, Vinicius Augusto
author_facet Dias Filho, Vinicius Augusto
author_role author
dc.contributor.author.fl_str_mv Dias Filho, Vinicius Augusto
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2964724004718343
dc.contributor.advisor1.fl_str_mv Maria, Alexandre Nizio
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7138924715876235
contributor_str_mv Maria, Alexandre Nizio
dc.subject.por.fl_str_mv Cinética espermática
Crioprotetor
Gema de ovo
Metilglicol
Peixe
Zootecnia
Tambaqui
Criopreservação de orgãos, tecidos, etc
Reprodução de peixes
Reprodução animal
topic Cinética espermática
Crioprotetor
Gema de ovo
Metilglicol
Peixe
Zootecnia
Tambaqui
Criopreservação de orgãos, tecidos, etc
Reprodução de peixes
Reprodução animal
Crioprotector
Egg yolk
Fish
Methylglycol
Sperm kinetics
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
dc.subject.eng.fl_str_mv Crioprotector
Egg yolk
Fish
Methylglycol
Sperm kinetics
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
description Cryopreservation of fish semen is a key to optimization of reproductive management, the formation of germplasm bank and the development of genetic improvement programs. The success of this technique depends on the management and balance of several factors such as the collection, dilution, packaging, freezing and thawing. Tambaqui semen cryopreservation protocols have been developed using 0.5 mL straws on packaging. However, the high volumes of semen produced and the elevated taxes of fecundity are presented by this specie, it is necessary to use larger volume storage containers for application to large-scale production. The objective of this study was to establish a tambaqui semen cryopreservation protocol in large straw 4,0mL, from the definition of cryosolution, equilibration time and the best ratio between temperature and time semen thawing. The semen collected was diluted, packaged in large straws, frozen in liquid nitrogen which was conditioned in dry-shipper and stored in cryogenic cylinder at -196 °C. In the first experiment, different cryosolutions (M1 - 5% 5% methylglycol; M2 - 5% 5% methylglycol+ 5% egg yolk; M3 - 10% 5% methylglycol; M4 - 10% 5% methylglycol + 5% egg yolk; M5 - 15% 5% methylglycol; M6 - 15% de 5% methylglycol + 5% egg yolk) and three equilibration times (4, 20 and 40 minutes) were tested. In the second experiment, different relation between temperature and time thawing on sperm quality (30ºC for 50s - T1; 30ºC for 80s - T2; 60ºC for 25s - T3 e 60ºC for 40s - T4) were evaluated. In conclusion, this study showed that the ideal protocol for cryopreservation of tambaqui semen in large straw 4,0mL is made by diluting semen at a ratio of 1: 9 (v: v) in a medium composed of 5% methylglycol and 5% egg yolk. Both of them must have remained in contact with each other for 4 minutes until both were subjected to freezing in dry-shipper. The thawing of samples required to be performed in a water bath at 60 ° C for 25s.
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-25
dc.date.accessioned.fl_str_mv 2017-09-27T18:04:11Z
dc.date.available.fl_str_mv 2017-09-27T18:04:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv DIAS FILHO, Vinicius Augusto. Cryopreservation of tambaqui (Colossoma macropomum) semen in large straws. 2015. 43 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Sergipe, São Cristóvão, 2015.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/handle/riufs/6378
identifier_str_mv DIAS FILHO, Vinicius Augusto. Cryopreservation of tambaqui (Colossoma macropomum) semen in large straws. 2015. 43 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Sergipe, São Cristóvão, 2015.
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