Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | http://ri.ufs.br/jspui/handle/riufs/8555 |
Resumo: | Introduction: The high morbidity, mortality of inhaled injury victims, as well as the costs of their care, underscore the need to invest in effective and low cost treatments that contain the local and systemic pathological process. Carvacrol is a natural product with great therapeutic potential for this condition since it has antioxidant and anti-inflammatory properties. Objective: To evaluate the effect of complexed beta-cyclodextrin (β-CD) carvacrol on aerosolized rats exposed to smoke inhalation injury, considering the toxicity, antioxidant effects and histomorphological aspects of the trachea, lung and liver. Materials and methods: A randomized, longitudinal experimental study with a control group in which 24 adult rats with regular estrous cycle were divided into 4 groups: Control (without injury and without treatment), oxygen with physiological saline (GOS), oxygen with carvacrol (GOCJ) and oxygen with ultrasonic carvacrol (GOCU), with six rats per group. About 48 hours after the lung injury the rats were euthanized. The tests applied were from Shapiro-Wilk, Anova Test One Way (normal distributions), Kruskal-Wallis Test (abnormal distributions) and Dunnet's post test. The level of significance was 95% (p <0.05). Histomorphological analysis was performed in a descriptive way. Results: Variations in the hemoglobin, hematocrit, TGP / ALT, total bilirubin, creatinine and urea rates remained within the normal range. There were no differences in the MPO and TBARS levels between the groups (p> 0.05), however the levels of GSH and FRAP in the GOCJ (p <0.01 and <0.05) and COCU (p <0, 01 and p <0.01), were greater than the GOS, similar to Control. GOCU had greater preservation of tracheal epithelium, lower inflammatory response, reduction of areas of pulmonary emphysema and thickness of alveolar septa. Carvacrol has not produced any toxic cellular or hepatic effects. Conclusion: Carvacrol complexed in β-CD administered by the inhalation route, at the dose and concentration administered, presented antioxidant action, ameliorated tracheal lesions and pulmonary emphysema, without causing hepatotoxicity. |
id |
UFS-2_bee0ae6ab6efbc4187e6d2c6f000fab0 |
---|---|
oai_identifier_str |
oai:ufs.br:riufs/8555 |
network_acronym_str |
UFS-2 |
network_name_str |
Repositório Institucional da UFS |
repository_id_str |
|
spelling |
Silva, Érika RamosAraújo, Adriano Antunes de Souza2018-07-09T22:10:02Z2018-07-09T22:10:02Z2018-01-19SILVA, Érika Ramos. Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça. 2018. 77 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Sergipe, Aracaju, 2018.http://ri.ufs.br/jspui/handle/riufs/8555Introduction: The high morbidity, mortality of inhaled injury victims, as well as the costs of their care, underscore the need to invest in effective and low cost treatments that contain the local and systemic pathological process. Carvacrol is a natural product with great therapeutic potential for this condition since it has antioxidant and anti-inflammatory properties. Objective: To evaluate the effect of complexed beta-cyclodextrin (β-CD) carvacrol on aerosolized rats exposed to smoke inhalation injury, considering the toxicity, antioxidant effects and histomorphological aspects of the trachea, lung and liver. Materials and methods: A randomized, longitudinal experimental study with a control group in which 24 adult rats with regular estrous cycle were divided into 4 groups: Control (without injury and without treatment), oxygen with physiological saline (GOS), oxygen with carvacrol (GOCJ) and oxygen with ultrasonic carvacrol (GOCU), with six rats per group. About 48 hours after the lung injury the rats were euthanized. The tests applied were from Shapiro-Wilk, Anova Test One Way (normal distributions), Kruskal-Wallis Test (abnormal distributions) and Dunnet's post test. The level of significance was 95% (p <0.05). Histomorphological analysis was performed in a descriptive way. Results: Variations in the hemoglobin, hematocrit, TGP / ALT, total bilirubin, creatinine and urea rates remained within the normal range. There were no differences in the MPO and TBARS levels between the groups (p> 0.05), however the levels of GSH and FRAP in the GOCJ (p <0.01 and <0.05) and COCU (p <0, 01 and p <0.01), were greater than the GOS, similar to Control. GOCU had greater preservation of tracheal epithelium, lower inflammatory response, reduction of areas of pulmonary emphysema and thickness of alveolar septa. Carvacrol has not produced any toxic cellular or hepatic effects. Conclusion: Carvacrol complexed in β-CD administered by the inhalation route, at the dose and concentration administered, presented antioxidant action, ameliorated tracheal lesions and pulmonary emphysema, without causing hepatotoxicity.Introdução: A elevada morbidade, mortalidade das vítimas de lesão inalatória, bem como os custos com sua assistência, ressaltam a necessidade de investimento em tratamentos eficazes e de baixo custo que contenham o processo patológico local e sistêmico. O carvacrol é produto natural, com grande potencial terapêutico para esta afecção uma vez que possui propriedades antioxidantes e antiinflamatórias. Objetivos: Avaliar o efeito do carvacrol complexado em betaciclodextrina (β-CD), administrado sob forma de aerossóis, sobre ratas submetidas à lesão por inalação de fumaça, considerando a toxicidade, os efeitos antioxidantes e os aspectos histomorfológicos da traquéia, pulmão e fígado. Materiais e Método: Estudo experimental longitudinal, randomizado com grupo controle, no qual 24 ratas adultas com ciclo estral regular foram distribuídas em 4 grupos: Controle (sem lesão e sem tratamento), oxigênio com soro fisiológico (GOS), oxigênio com carvacrol a jato (GOCJ) e oxigênio com carvacrol ultrassônico (GOCU), sendo seis ratas por grupo. Cerca de 48h após a lesão pulmonar as ratas foram eutanasiadas. Os testes aplicados foram de Shapiro-Wilk, Teste Anova One Way (distribuições normais), Teste Kruskal-Wallis (distribuições anormais) e post test de Dunnet. O nível de significância foi 95% (p<0.05). A análise histomorfológica foi realizada de maneira descritiva. Resultados: As variações nas taxas de hemoglobina, hematócrito, TGP/ALT, bilirrubina total, creatinina e ureia, mantiveram-se na faixa de normalidade. Não houve diferença nos níveis de MPO e TBARS entre os grupos (p>0,05), entretanto os níveis de GSH e FRAP, nos grupos GOCJ (p<0,01 e p<0,05) e COCU (p<0,01 e p<0,01), foram maiores que o GOS, semelhantes ao Controle. O GOCU teve maior preservação do epitélio traqueal, menor resposta inflamatória, redução das áreas de enfisema pulmonar e espessura dos septos alveolares. Carvacrol não gerou efeitos tóxicos celulares ou hepáticos. Conclusão: O carvacrol complexado em β-CD administrado pela via inalatória, na dose e concentração administradas, apresentou ação antioxidante, amenizou lesões traqueais e enfisema pulmonar, sem ocasionar hepatotoxidade.AracajuporCiências da saúdeFumaçaMonoterpenoAntioxidantesCiclodextrinasTerapia inalatóriaSmokeMonoterpeneAntioxidantsCyclodextrinsInhalation therapyCIENCIAS DA SAUDEEfeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaçaEffects of complexed carvacrol on betacyclodextrin on the inflammatory reaction of rats subjected to smoke inhalation injuryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPós-Graduação em Ciências da SaúdeUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTEXTERIKA_RAMOS_SILVA.pdf.txtERIKA_RAMOS_SILVA.pdf.txtExtracted texttext/plain123680https://ri.ufs.br/jspui/bitstream/riufs/8555/3/ERIKA_RAMOS_SILVA.pdf.txt8db2fe741daed0ca3328b76e07c91460MD53THUMBNAILERIKA_RAMOS_SILVA.pdf.jpgERIKA_RAMOS_SILVA.pdf.jpgGenerated Thumbnailimage/jpeg1264https://ri.ufs.br/jspui/bitstream/riufs/8555/4/ERIKA_RAMOS_SILVA.pdf.jpgc6dd77df2a196de0257caf5848cb0385MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/8555/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALERIKA_RAMOS_SILVA.pdfERIKA_RAMOS_SILVA.pdfapplication/pdf1949572https://ri.ufs.br/jspui/bitstream/riufs/8555/2/ERIKA_RAMOS_SILVA.pdfed406f1905b102edff08219522974cf6MD52riufs/85552018-07-09 20:48:12.244oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-07-09T23:48:12Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça |
dc.title.alternative.eng.fl_str_mv |
Effects of complexed carvacrol on betacyclodextrin on the inflammatory reaction of rats subjected to smoke inhalation injury |
title |
Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça |
spellingShingle |
Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça Silva, Érika Ramos Ciências da saúde Fumaça Monoterpeno Antioxidantes Ciclodextrinas Terapia inalatória Smoke Monoterpene Antioxidants Cyclodextrins Inhalation therapy CIENCIAS DA SAUDE |
title_short |
Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça |
title_full |
Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça |
title_fullStr |
Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça |
title_full_unstemmed |
Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça |
title_sort |
Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça |
author |
Silva, Érika Ramos |
author_facet |
Silva, Érika Ramos |
author_role |
author |
dc.contributor.author.fl_str_mv |
Silva, Érika Ramos |
dc.contributor.advisor1.fl_str_mv |
Araújo, Adriano Antunes de Souza |
contributor_str_mv |
Araújo, Adriano Antunes de Souza |
dc.subject.por.fl_str_mv |
Ciências da saúde Fumaça Monoterpeno Antioxidantes Ciclodextrinas Terapia inalatória |
topic |
Ciências da saúde Fumaça Monoterpeno Antioxidantes Ciclodextrinas Terapia inalatória Smoke Monoterpene Antioxidants Cyclodextrins Inhalation therapy CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
Smoke Monoterpene Antioxidants Cyclodextrins Inhalation therapy |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Introduction: The high morbidity, mortality of inhaled injury victims, as well as the costs of their care, underscore the need to invest in effective and low cost treatments that contain the local and systemic pathological process. Carvacrol is a natural product with great therapeutic potential for this condition since it has antioxidant and anti-inflammatory properties. Objective: To evaluate the effect of complexed beta-cyclodextrin (β-CD) carvacrol on aerosolized rats exposed to smoke inhalation injury, considering the toxicity, antioxidant effects and histomorphological aspects of the trachea, lung and liver. Materials and methods: A randomized, longitudinal experimental study with a control group in which 24 adult rats with regular estrous cycle were divided into 4 groups: Control (without injury and without treatment), oxygen with physiological saline (GOS), oxygen with carvacrol (GOCJ) and oxygen with ultrasonic carvacrol (GOCU), with six rats per group. About 48 hours after the lung injury the rats were euthanized. The tests applied were from Shapiro-Wilk, Anova Test One Way (normal distributions), Kruskal-Wallis Test (abnormal distributions) and Dunnet's post test. The level of significance was 95% (p <0.05). Histomorphological analysis was performed in a descriptive way. Results: Variations in the hemoglobin, hematocrit, TGP / ALT, total bilirubin, creatinine and urea rates remained within the normal range. There were no differences in the MPO and TBARS levels between the groups (p> 0.05), however the levels of GSH and FRAP in the GOCJ (p <0.01 and <0.05) and COCU (p <0, 01 and p <0.01), were greater than the GOS, similar to Control. GOCU had greater preservation of tracheal epithelium, lower inflammatory response, reduction of areas of pulmonary emphysema and thickness of alveolar septa. Carvacrol has not produced any toxic cellular or hepatic effects. Conclusion: Carvacrol complexed in β-CD administered by the inhalation route, at the dose and concentration administered, presented antioxidant action, ameliorated tracheal lesions and pulmonary emphysema, without causing hepatotoxicity. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-07-09T22:10:02Z |
dc.date.available.fl_str_mv |
2018-07-09T22:10:02Z |
dc.date.issued.fl_str_mv |
2018-01-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Érika Ramos. Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça. 2018. 77 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Sergipe, Aracaju, 2018. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/8555 |
identifier_str_mv |
SILVA, Érika Ramos. Efeitos do carvacrol complexado em betaciclodextrina sobre a reação inflamatória de ratas submetidas à lesão por inalação de fumaça. 2018. 77 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Sergipe, Aracaju, 2018. |
url |
http://ri.ufs.br/jspui/handle/riufs/8555 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.program.fl_str_mv |
Pós-Graduação em Ciências da Saúde |
dc.publisher.initials.fl_str_mv |
Universidade Federal de Sergipe |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS |
instname_str |
Universidade Federal de Sergipe (UFS) |
instacron_str |
UFS |
institution |
UFS |
reponame_str |
Repositório Institucional da UFS |
collection |
Repositório Institucional da UFS |
bitstream.url.fl_str_mv |
https://ri.ufs.br/jspui/bitstream/riufs/8555/3/ERIKA_RAMOS_SILVA.pdf.txt https://ri.ufs.br/jspui/bitstream/riufs/8555/4/ERIKA_RAMOS_SILVA.pdf.jpg https://ri.ufs.br/jspui/bitstream/riufs/8555/1/license.txt https://ri.ufs.br/jspui/bitstream/riufs/8555/2/ERIKA_RAMOS_SILVA.pdf |
bitstream.checksum.fl_str_mv |
8db2fe741daed0ca3328b76e07c91460 c6dd77df2a196de0257caf5848cb0385 098cbbf65c2c15e1fb2e49c5d306a44c ed406f1905b102edff08219522974cf6 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS) |
repository.mail.fl_str_mv |
repositorio@academico.ufs.br |
_version_ |
1802110659298590720 |