Citotoxicidade de medicações intracanais em fibroblastos L929

Detalhes bibliográficos
Autor(a) principal: Farias, Michelle de Paula
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/5893
Resumo: Intracanal dressings are substances used during endodontic treatment and requiring biocompatible with periradicular tissues, not causing tissue damage or interfere in repair process. This study aimed to assess the cytotoxicity of endodontic dressing when in contact with L929 mouse fibroblast cells, after different periods. To assess the cytotoxicity of three intracanal dressings and three vehicles were divided into seven groups, namely: calcium hydroxide/camphorated paramonochlorophenol/ glycerin (CPG); iodoform/ glycerin (IG); calcium hydroxide/iodoform/distilled water (CIW); iodoform/distilled water (IW); calcium hydroxide/distilled water (CW); Otosporin® (OT); and control group. The eluates of the medications were prepared and placed in contact with the cells (1 x 105 cells/well) during periods of 24, 48, 72hours, 5 and 7 days. After each evaluation time, a colorimetric assay was conducted using methyl tetrazolium reagent (MTT) and the reading of the plates was performed in a spectrophotometer with optical density of 570 nm. Data were tabulated and subjected to statistical analysis by ANOVA-Tukey test with a significance level of 5%. At 24hours, IG and OT (P <0.001) showed greater cytotoxicity, as also observed by the OT group (P <0.001) when analyzed in 48hours. In 72 hours, it could be observed that the CW and OT groups were more cytotoxic compared to the control group. These data were also demonstrated in groups CPG, IW, CW and OT in 5 days. Within 7 days all drugs showed cytotoxicity, but OT group showed the most cytotoxic (P <0.001). With respect to experimental time factor was observed in all groups significant differences between 24hours and 7 days. It is concluded that all medications showed cytotoxicity at some point in the research being Otosporin ® the most cytotoxic and that time influenced the cytotoxicity of all medications analyzed intracanal medication.
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spelling Farias, Michelle de PaulaRibeiro, Maria Amália Gonzagahttp://lattes.cnpq.br/29056942431296992017-09-27T13:50:39Z2017-09-27T13:50:39Z2014-02-27Farias, Michelle de Paula. Citotoxicidade de medicações intracanais em fibroblastos L929. 2014. 55 f. Dissertação (Pós-Graduação em Odontologia) - Universidade Federal de Sergipe, Aracaju, 2014.https://ri.ufs.br/handle/riufs/5893Intracanal dressings are substances used during endodontic treatment and requiring biocompatible with periradicular tissues, not causing tissue damage or interfere in repair process. This study aimed to assess the cytotoxicity of endodontic dressing when in contact with L929 mouse fibroblast cells, after different periods. To assess the cytotoxicity of three intracanal dressings and three vehicles were divided into seven groups, namely: calcium hydroxide/camphorated paramonochlorophenol/ glycerin (CPG); iodoform/ glycerin (IG); calcium hydroxide/iodoform/distilled water (CIW); iodoform/distilled water (IW); calcium hydroxide/distilled water (CW); Otosporin® (OT); and control group. The eluates of the medications were prepared and placed in contact with the cells (1 x 105 cells/well) during periods of 24, 48, 72hours, 5 and 7 days. After each evaluation time, a colorimetric assay was conducted using methyl tetrazolium reagent (MTT) and the reading of the plates was performed in a spectrophotometer with optical density of 570 nm. Data were tabulated and subjected to statistical analysis by ANOVA-Tukey test with a significance level of 5%. At 24hours, IG and OT (P <0.001) showed greater cytotoxicity, as also observed by the OT group (P <0.001) when analyzed in 48hours. In 72 hours, it could be observed that the CW and OT groups were more cytotoxic compared to the control group. These data were also demonstrated in groups CPG, IW, CW and OT in 5 days. Within 7 days all drugs showed cytotoxicity, but OT group showed the most cytotoxic (P <0.001). With respect to experimental time factor was observed in all groups significant differences between 24hours and 7 days. It is concluded that all medications showed cytotoxicity at some point in the research being Otosporin ® the most cytotoxic and that time influenced the cytotoxicity of all medications analyzed intracanal medication.As medicações intracanais são substâncias utilizadas no tratamento endodôntico que necessitam apresentar biocompatibilidade com os tecidos perirradiculares, de modo a não causar danos teciduais e nem interferir no processo de reparo. O estudo objetivou avaliar a citotoxicidade de medicamentos de uso endodôntico, quando em contato com células fibroblásticas de murinos L929, em diferentes períodos de observação. Para avaliar a citotoxicidade, foram utilizadas três medicamentos e três veículos, divididos em sete grupos experimentais, a saber: hidróxido de cálcio-paramonoclorofenol canforado-glicerina (HPG); iodofórmio-glicerina (IG); hidróxido de cálcio-iodofórmio-água destilada (HCI); iodofórmio-água destilada (IA); hidróxido de cálcio-água destilada (HC); Otosporin® (OT) e grupo controle - composto por células e meio de cultura. Foram preparados os eluatos das medicações sendo estes colocados em contato com as células (1 x 105 células/poço) por períodos de 24h, 48h, 72h, 5 e 7 dias. Após cada tempo experimental, foi executado o ensaio colorimétrico, utilizando o reagente metiltetrazólio (MTT) e a leitura das placas foi realizada no espectrofotômetro utilizando a densidade óptica de 570nm. Os dados foram tabulados e submetidos a análise por meio da ANOVA-Tukey, com um nível de significância de 5%. Em 24h, IG e OT (P<0,001) demonstraram maior citotoxicidade, fato este também observado pelo grupo OT (P<0,001) quando analisado em 48h. Em 72h, pôde-se observar que os grupos HC e OT foram mais citotóxicos quando comparados ao grupo controle. Estes resultados também foram demonstrados nos grupos HPG, IA, HC e OT em 5 dias. Em 7 dias todas as medicações apresentaram citotoxicidade, porém o grupo OT mostrou-se mais citotóxico (P<0,001). Com relação ao fator tempo experimental, foi observado em todos os grupos diferença significativa entre 24h e 7 dias. Conclui-se que todas as medicações apresentaram citotoxicidade em algum momento da pesquisa sendo o Otosporin® a medicação intracanal mais citotóxica e, que o tempo influenciou na citotoxicidade de todas medicações analisadas.application/pdfporUniversidade Federal de SergipePós-Graduação em OdontologiaUFSBrasilOdontologiaCitotoxicidadeEndodontiaHidróxido de cálcioIodofórmioFibroblastosCytotoxicityEndodonticsCalcium hydroxideIodoformiumFibroblastsCIENCIAS DA SAUDE::ODONTOLOGIACitotoxicidade de medicações intracanais em fibroblastos L929info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSORIGINALMICHELLE_PAULA_FARIAS.pdfapplication/pdf923824https://ri.ufs.br/jspui/bitstream/riufs/5893/1/MICHELLE_PAULA_FARIAS.pdfff9a91fcaaac400d586453d59ef4e09bMD51TEXTMICHELLE_PAULA_FARIAS.pdf.txtMICHELLE_PAULA_FARIAS.pdf.txtExtracted texttext/plain93322https://ri.ufs.br/jspui/bitstream/riufs/5893/2/MICHELLE_PAULA_FARIAS.pdf.txt5ab86bddc5bfd4941c140e3ef8c99a5bMD52THUMBNAILMICHELLE_PAULA_FARIAS.pdf.jpgMICHELLE_PAULA_FARIAS.pdf.jpgGenerated Thumbnailimage/jpeg1134https://ri.ufs.br/jspui/bitstream/riufs/5893/3/MICHELLE_PAULA_FARIAS.pdf.jpg13f866e3083befef12ce1b3197ad0ef4MD53riufs/58932017-12-21 21:40:47.336oai:ufs.br:riufs/5893Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-12-22T00:40:47Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.por.fl_str_mv Citotoxicidade de medicações intracanais em fibroblastos L929
title Citotoxicidade de medicações intracanais em fibroblastos L929
spellingShingle Citotoxicidade de medicações intracanais em fibroblastos L929
Farias, Michelle de Paula
Odontologia
Citotoxicidade
Endodontia
Hidróxido de cálcio
Iodofórmio
Fibroblastos
Cytotoxicity
Endodontics
Calcium hydroxide
Iodoformium
Fibroblasts
CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Citotoxicidade de medicações intracanais em fibroblastos L929
title_full Citotoxicidade de medicações intracanais em fibroblastos L929
title_fullStr Citotoxicidade de medicações intracanais em fibroblastos L929
title_full_unstemmed Citotoxicidade de medicações intracanais em fibroblastos L929
title_sort Citotoxicidade de medicações intracanais em fibroblastos L929
author Farias, Michelle de Paula
author_facet Farias, Michelle de Paula
author_role author
dc.contributor.author.fl_str_mv Farias, Michelle de Paula
dc.contributor.advisor1.fl_str_mv Ribeiro, Maria Amália Gonzaga
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2905694243129699
contributor_str_mv Ribeiro, Maria Amália Gonzaga
dc.subject.por.fl_str_mv Odontologia
Citotoxicidade
Endodontia
Hidróxido de cálcio
Iodofórmio
Fibroblastos
topic Odontologia
Citotoxicidade
Endodontia
Hidróxido de cálcio
Iodofórmio
Fibroblastos
Cytotoxicity
Endodontics
Calcium hydroxide
Iodoformium
Fibroblasts
CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.eng.fl_str_mv Cytotoxicity
Endodontics
Calcium hydroxide
Iodoformium
Fibroblasts
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE::ODONTOLOGIA
description Intracanal dressings are substances used during endodontic treatment and requiring biocompatible with periradicular tissues, not causing tissue damage or interfere in repair process. This study aimed to assess the cytotoxicity of endodontic dressing when in contact with L929 mouse fibroblast cells, after different periods. To assess the cytotoxicity of three intracanal dressings and three vehicles were divided into seven groups, namely: calcium hydroxide/camphorated paramonochlorophenol/ glycerin (CPG); iodoform/ glycerin (IG); calcium hydroxide/iodoform/distilled water (CIW); iodoform/distilled water (IW); calcium hydroxide/distilled water (CW); Otosporin® (OT); and control group. The eluates of the medications were prepared and placed in contact with the cells (1 x 105 cells/well) during periods of 24, 48, 72hours, 5 and 7 days. After each evaluation time, a colorimetric assay was conducted using methyl tetrazolium reagent (MTT) and the reading of the plates was performed in a spectrophotometer with optical density of 570 nm. Data were tabulated and subjected to statistical analysis by ANOVA-Tukey test with a significance level of 5%. At 24hours, IG and OT (P <0.001) showed greater cytotoxicity, as also observed by the OT group (P <0.001) when analyzed in 48hours. In 72 hours, it could be observed that the CW and OT groups were more cytotoxic compared to the control group. These data were also demonstrated in groups CPG, IW, CW and OT in 5 days. Within 7 days all drugs showed cytotoxicity, but OT group showed the most cytotoxic (P <0.001). With respect to experimental time factor was observed in all groups significant differences between 24hours and 7 days. It is concluded that all medications showed cytotoxicity at some point in the research being Otosporin ® the most cytotoxic and that time influenced the cytotoxicity of all medications analyzed intracanal medication.
publishDate 2014
dc.date.issued.fl_str_mv 2014-02-27
dc.date.accessioned.fl_str_mv 2017-09-27T13:50:39Z
dc.date.available.fl_str_mv 2017-09-27T13:50:39Z
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dc.identifier.citation.fl_str_mv Farias, Michelle de Paula. Citotoxicidade de medicações intracanais em fibroblastos L929. 2014. 55 f. Dissertação (Pós-Graduação em Odontologia) - Universidade Federal de Sergipe, Aracaju, 2014.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/handle/riufs/5893
identifier_str_mv Farias, Michelle de Paula. Citotoxicidade de medicações intracanais em fibroblastos L929. 2014. 55 f. Dissertação (Pós-Graduação em Odontologia) - Universidade Federal de Sergipe, Aracaju, 2014.
url https://ri.ufs.br/handle/riufs/5893
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publisher.none.fl_str_mv Universidade Federal de Sergipe
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