Potencial antifúngico de frações do gel de aloe vera (l.) burm. f. frente à candida albicans

• Autor(a) principal: Santos, Kananda Franciele Souza
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/12352
• topic: Babosa; Partição líquido-líquido trifásica; Biofilme; Babosa (Planta); Antimicóticos; Three-phase liquid-liquid partition; Bradford; Biofilm

The species Aloe vera (L.) Burm.F. (Xanthorroeaceae), popularly known as slug, has been used since antiquity for various purposes. From its most used part, leaf gel, was discovered a protein of molecular mass 14 kDa that has a potent antifungal action against Candida albicans. Considering that the therapeutic options available to treat fungal infections present limitations, it is sought for substances that, in addition to inhibiting microbial growth, combat their virulence factors, aiding in the treatment of infections. Thus, the objective of the present work was to obtain protein fractions of A. vera, to characterize them and to evaluate the antifungal action against Candida albicans, as well as to evaluate the effect of inhibition of biofilm formation. The process of obtaining A. vera gel proteins was performed using three-phase liquid-liquid partitioning and dialysis techniques. In the first step of the process, a three-phase liquid-liquid partition was performed using t-butanol, tris-hydroxymethylaminomethane buffer (TRIS, 50 mM, pH 8.0) and ammonium sulfate solution (1.6 M). The fraction obtained after this step was dissolved in TRIS buffer and dialyzed for a period of 24 h at 4 ° C under constant stirring. The two fractions obtained after dialysis were lyophilized and quantified by the Bradford method. The antifungal activity assays were conducted by the 96-well plate microdilution method, and then the biofilm formation evaluation was performed with the same plate. It was not possible to detect / quantify the proteins present in the fractions, however by an NMR analysis the presence ofsugars was verified. These fractions at the following concentrations of 250; 125; 31.2 and 7.8 µg / mL, presented antifungal activity with respect to the inhibition of C.albicans biofilm formation, inhibiting about 70% of it. Thus it is expected that these results may contribute to the discovery of new prototypes to antifungal drugs.