In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/1586 |
Resumo: | The macambira is an ornamental plant that has a high degree of ecological importance. The objective in this research was to evaluate the in vitro germination of macambira in different culture media and establish the best substrate for the acclimatization of micropropagated plants. Five treatments were tested: 0, 25, 50, 75, and 100% salt of Murashige and Skoog (MS) medium. We calculated the percentage of germination and germination speed index (GSI). For testing acclimatization, five combinations of substrates were evaluated: coconut coir : earthworm castings (2:1); coconut coir : earthworm castings: sand (1:1:1, 2:1:1 and 2:2:1); and vermiculite : earthworm castings (2:1). There was no difference between the concentrations of MS salts based on the percentage of germination. For the GSI, the treatment 100% MS salts had the poorest results. There was no significant difference in the acclimatization of seedlings for the variables number of leaves and dried mass of roots and shoots. For the variable length of the longest root, coconut coir: earthworm castings (2:1) and coconut coir : earthworm castings : sand (2:1:1 and 2:2:1) had the highest averages, and for dried root mass, the coconut coir : earthworm castings : sand (1:1:1 and 2:2:1) provided the best results. Germination can be carried out in vitro in a medium containing only water and agar, and for acclimatization, the substrate coconut coir : earthworm castings : sand (2:2:1) should be used._________________________________________________________________________________________ RESUMO: A macambira é uma planta ornamental, que apresenta elevado grau de importância ecológica nos ecossistemas. O objetivo deste trabalho foi avaliar a germinação in vitro da macambira em função de diferentes meios de cultura, além de estabelecer o melhor substrato para a aclimatização de mudas micropropagadas. Para tanto, foram testados cinco tratamentos (DIC), com 100, 75, 50, 25 e 0% dos sais do meio MS. Foi estimada a porcentagem de germinação e verificado o índice de velocidade de germinação (IVG). Para aclimatização, foram avaliadas cinco combinações de substratos: pó de coco : húmus de minhoca (2:1); pó de coco : húmus de minhoca : areia (1:1:1), (2:1:1), (2:2:1), e vermiculita: húmus de minhoca (2:1). Não houve diferença entre as concentrações de sais MS quanto à porcentagem de germinação. Quanto ao IVG, o tratamento 100% de sais MS apresentou o pior resultado. Na aclimatização das mudas, para as variáveis ‘número de folhas e de raízes’ e ‘matéria seca de parte aérea’, não houve diferença. Para a variável ‘comprimento da maior raiz’, os substratos pó de coco : húmus de minhoca (2:1) e pó de coco : húmus de minhoca : areia (2:1:1) e (2:2:1) apresentaram as maiores médias e, para matéria seca de raízes, os substratos pó de coco : húmus de minhoca : areia (1:1:1) e (2:2:1) proporcionaram os melhores resultados. A germinação in vitro pode ser realizada em meio contendo apenas água e ágar; para a aclimatização, deve-se utilizar o substrato pó de coco : húmus de minhoca : areia (2:2:1). |
id |
UFS-2_e2446d84fbc5457ba20a86f695cab211 |
---|---|
oai_identifier_str |
oai:ufs.br:riufs/1586 |
network_acronym_str |
UFS-2 |
network_name_str |
Repositório Institucional da UFS |
repository_id_str |
|
spelling |
Arrigoni-Blank, Maria de FátimaSantos, Franciane dosOliveira, Ana Catarina Lima deBlank, Arie FitzgeraldSantos, Mariana de SouzaMenezes, Thays Saynara Alves2016-01-14T16:59:41Z2016-01-14T16:59:41Z2013ARRIGONI-BLANK, M. F. et al. In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings. Revista de Ciências Agrárias, Belém, v. 56, 2013. Suplemento. Disponível em: <https://periodicos.ufra.edu.br/index.php?journal=ajaes&page=article&op=view&path%5B%5D=1433&path%5B%5D=453>. Acesso em: 14 jan. 2016.2177-8760https://ri.ufs.br/handle/riufs/1586Licença Creative CommonsThe macambira is an ornamental plant that has a high degree of ecological importance. The objective in this research was to evaluate the in vitro germination of macambira in different culture media and establish the best substrate for the acclimatization of micropropagated plants. Five treatments were tested: 0, 25, 50, 75, and 100% salt of Murashige and Skoog (MS) medium. We calculated the percentage of germination and germination speed index (GSI). For testing acclimatization, five combinations of substrates were evaluated: coconut coir : earthworm castings (2:1); coconut coir : earthworm castings: sand (1:1:1, 2:1:1 and 2:2:1); and vermiculite : earthworm castings (2:1). There was no difference between the concentrations of MS salts based on the percentage of germination. For the GSI, the treatment 100% MS salts had the poorest results. There was no significant difference in the acclimatization of seedlings for the variables number of leaves and dried mass of roots and shoots. For the variable length of the longest root, coconut coir: earthworm castings (2:1) and coconut coir : earthworm castings : sand (2:1:1 and 2:2:1) had the highest averages, and for dried root mass, the coconut coir : earthworm castings : sand (1:1:1 and 2:2:1) provided the best results. Germination can be carried out in vitro in a medium containing only water and agar, and for acclimatization, the substrate coconut coir : earthworm castings : sand (2:2:1) should be used._________________________________________________________________________________________ RESUMO: A macambira é uma planta ornamental, que apresenta elevado grau de importância ecológica nos ecossistemas. O objetivo deste trabalho foi avaliar a germinação in vitro da macambira em função de diferentes meios de cultura, além de estabelecer o melhor substrato para a aclimatização de mudas micropropagadas. Para tanto, foram testados cinco tratamentos (DIC), com 100, 75, 50, 25 e 0% dos sais do meio MS. Foi estimada a porcentagem de germinação e verificado o índice de velocidade de germinação (IVG). Para aclimatização, foram avaliadas cinco combinações de substratos: pó de coco : húmus de minhoca (2:1); pó de coco : húmus de minhoca : areia (1:1:1), (2:1:1), (2:2:1), e vermiculita: húmus de minhoca (2:1). Não houve diferença entre as concentrações de sais MS quanto à porcentagem de germinação. Quanto ao IVG, o tratamento 100% de sais MS apresentou o pior resultado. Na aclimatização das mudas, para as variáveis ‘número de folhas e de raízes’ e ‘matéria seca de parte aérea’, não houve diferença. Para a variável ‘comprimento da maior raiz’, os substratos pó de coco : húmus de minhoca (2:1) e pó de coco : húmus de minhoca : areia (2:1:1) e (2:2:1) apresentaram as maiores médias e, para matéria seca de raízes, os substratos pó de coco : húmus de minhoca : areia (1:1:1) e (2:2:1) proporcionaram os melhores resultados. A germinação in vitro pode ser realizada em meio contendo apenas água e ágar; para a aclimatização, deve-se utilizar o substrato pó de coco : húmus de minhoca : areia (2:2:1).Universidade Federal Rural da AmazôniaBromeliaceaeMS MediumSubstrateMeio MSSubstratoBroméliasMacambiraIn vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlingsGerminação in vitro de sementes e aclimatização de mudas de Macambira (Bromelia laciniosa Martius ex Schultes f.)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILGerminationSeedsMacambira.pdf.jpgGerminationSeedsMacambira.pdf.jpgGenerated Thumbnailimage/jpeg1623https://ri.ufs.br/jspui/bitstream/riufs/1586/4/GerminationSeedsMacambira.pdf.jpg3a09dad6244b0114e562b3d20108e195MD54TEXTGerminationSeedsMacambira.pdf.txtGerminationSeedsMacambira.pdf.txtExtracted texttext/plain18131https://ri.ufs.br/jspui/bitstream/riufs/1586/3/GerminationSeedsMacambira.pdf.txt2b17162d205a87916619f8cdfacafaceMD53ORIGINALGerminationSeedsMacambira.pdfGerminationSeedsMacambira.pdfapplication/pdf765773https://ri.ufs.br/jspui/bitstream/riufs/1586/1/GerminationSeedsMacambira.pdf0b52464f9fb8b281cb92dab7bbae0adcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/1586/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52riufs/15862016-02-01 19:35:08.443oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2016-02-01T22:35:08Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings |
dc.title.alternative.pt_BR.fl_str_mv |
Germinação in vitro de sementes e aclimatização de mudas de Macambira (Bromelia laciniosa Martius ex Schultes f.) |
title |
In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings |
spellingShingle |
In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings Arrigoni-Blank, Maria de Fátima Bromeliaceae MS Medium Substrate Meio MS Substrato Bromélias Macambira |
title_short |
In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings |
title_full |
In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings |
title_fullStr |
In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings |
title_full_unstemmed |
In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings |
title_sort |
In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings |
author |
Arrigoni-Blank, Maria de Fátima |
author_facet |
Arrigoni-Blank, Maria de Fátima Santos, Franciane dos Oliveira, Ana Catarina Lima de Blank, Arie Fitzgerald Santos, Mariana de Souza Menezes, Thays Saynara Alves |
author_role |
author |
author2 |
Santos, Franciane dos Oliveira, Ana Catarina Lima de Blank, Arie Fitzgerald Santos, Mariana de Souza Menezes, Thays Saynara Alves |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Arrigoni-Blank, Maria de Fátima Santos, Franciane dos Oliveira, Ana Catarina Lima de Blank, Arie Fitzgerald Santos, Mariana de Souza Menezes, Thays Saynara Alves |
dc.subject.por.fl_str_mv |
Bromeliaceae MS Medium Substrate Meio MS Substrato Bromélias Macambira |
topic |
Bromeliaceae MS Medium Substrate Meio MS Substrato Bromélias Macambira |
description |
The macambira is an ornamental plant that has a high degree of ecological importance. The objective in this research was to evaluate the in vitro germination of macambira in different culture media and establish the best substrate for the acclimatization of micropropagated plants. Five treatments were tested: 0, 25, 50, 75, and 100% salt of Murashige and Skoog (MS) medium. We calculated the percentage of germination and germination speed index (GSI). For testing acclimatization, five combinations of substrates were evaluated: coconut coir : earthworm castings (2:1); coconut coir : earthworm castings: sand (1:1:1, 2:1:1 and 2:2:1); and vermiculite : earthworm castings (2:1). There was no difference between the concentrations of MS salts based on the percentage of germination. For the GSI, the treatment 100% MS salts had the poorest results. There was no significant difference in the acclimatization of seedlings for the variables number of leaves and dried mass of roots and shoots. For the variable length of the longest root, coconut coir: earthworm castings (2:1) and coconut coir : earthworm castings : sand (2:1:1 and 2:2:1) had the highest averages, and for dried root mass, the coconut coir : earthworm castings : sand (1:1:1 and 2:2:1) provided the best results. Germination can be carried out in vitro in a medium containing only water and agar, and for acclimatization, the substrate coconut coir : earthworm castings : sand (2:2:1) should be used._________________________________________________________________________________________ RESUMO: A macambira é uma planta ornamental, que apresenta elevado grau de importância ecológica nos ecossistemas. O objetivo deste trabalho foi avaliar a germinação in vitro da macambira em função de diferentes meios de cultura, além de estabelecer o melhor substrato para a aclimatização de mudas micropropagadas. Para tanto, foram testados cinco tratamentos (DIC), com 100, 75, 50, 25 e 0% dos sais do meio MS. Foi estimada a porcentagem de germinação e verificado o índice de velocidade de germinação (IVG). Para aclimatização, foram avaliadas cinco combinações de substratos: pó de coco : húmus de minhoca (2:1); pó de coco : húmus de minhoca : areia (1:1:1), (2:1:1), (2:2:1), e vermiculita: húmus de minhoca (2:1). Não houve diferença entre as concentrações de sais MS quanto à porcentagem de germinação. Quanto ao IVG, o tratamento 100% de sais MS apresentou o pior resultado. Na aclimatização das mudas, para as variáveis ‘número de folhas e de raízes’ e ‘matéria seca de parte aérea’, não houve diferença. Para a variável ‘comprimento da maior raiz’, os substratos pó de coco : húmus de minhoca (2:1) e pó de coco : húmus de minhoca : areia (2:1:1) e (2:2:1) apresentaram as maiores médias e, para matéria seca de raízes, os substratos pó de coco : húmus de minhoca : areia (1:1:1) e (2:2:1) proporcionaram os melhores resultados. A germinação in vitro pode ser realizada em meio contendo apenas água e ágar; para a aclimatização, deve-se utilizar o substrato pó de coco : húmus de minhoca : areia (2:2:1). |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2016-01-14T16:59:41Z |
dc.date.available.fl_str_mv |
2016-01-14T16:59:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARRIGONI-BLANK, M. F. et al. In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings. Revista de Ciências Agrárias, Belém, v. 56, 2013. Suplemento. Disponível em: <https://periodicos.ufra.edu.br/index.php?journal=ajaes&page=article&op=view&path%5B%5D=1433&path%5B%5D=453>. Acesso em: 14 jan. 2016. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/1586 |
dc.identifier.issn.none.fl_str_mv |
2177-8760 |
dc.identifier.license.pt_BR.fl_str_mv |
Licença Creative Commons |
identifier_str_mv |
ARRIGONI-BLANK, M. F. et al. In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings. Revista de Ciências Agrárias, Belém, v. 56, 2013. Suplemento. Disponível em: <https://periodicos.ufra.edu.br/index.php?journal=ajaes&page=article&op=view&path%5B%5D=1433&path%5B%5D=453>. Acesso em: 14 jan. 2016. 2177-8760 Licença Creative Commons |
url |
https://ri.ufs.br/handle/riufs/1586 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural da Amazônia |
publisher.none.fl_str_mv |
Universidade Federal Rural da Amazônia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS |
instname_str |
Universidade Federal de Sergipe (UFS) |
instacron_str |
UFS |
institution |
UFS |
reponame_str |
Repositório Institucional da UFS |
collection |
Repositório Institucional da UFS |
bitstream.url.fl_str_mv |
https://ri.ufs.br/jspui/bitstream/riufs/1586/4/GerminationSeedsMacambira.pdf.jpg https://ri.ufs.br/jspui/bitstream/riufs/1586/3/GerminationSeedsMacambira.pdf.txt https://ri.ufs.br/jspui/bitstream/riufs/1586/1/GerminationSeedsMacambira.pdf https://ri.ufs.br/jspui/bitstream/riufs/1586/2/license.txt |
bitstream.checksum.fl_str_mv |
3a09dad6244b0114e562b3d20108e195 2b17162d205a87916619f8cdfacaface 0b52464f9fb8b281cb92dab7bbae0adc 8a4605be74aa9ea9d79846c1fba20a33 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS) |
repository.mail.fl_str_mv |
repositorio@academico.ufs.br |
_version_ |
1802110669376454656 |