In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings

Detalhes bibliográficos
Autor(a) principal: Arrigoni-Blank, Maria de Fátima
Data de Publicação: 2013
Outros Autores: Santos, Franciane dos, Oliveira, Ana Catarina Lima de, Blank, Arie Fitzgerald, Santos, Mariana de Souza, Menezes, Thays Saynara Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/1586
Resumo: The macambira is an ornamental plant that has a high degree of ecological importance. The objective in this research was to evaluate the in vitro germination of macambira in different culture media and establish the best substrate for the acclimatization of micropropagated plants. Five treatments were tested: 0, 25, 50, 75, and 100% salt of Murashige and Skoog (MS) medium. We calculated the percentage of germination and germination speed index (GSI). For testing acclimatization, five combinations of substrates were evaluated: coconut coir : earthworm castings (2:1); coconut coir : earthworm castings: sand (1:1:1, 2:1:1 and 2:2:1); and vermiculite : earthworm castings (2:1). There was no difference between the concentrations of MS salts based on the percentage of germination. For the GSI, the treatment 100% MS salts had the poorest results. There was no significant difference in the acclimatization of seedlings for the variables number of leaves and dried mass of roots and shoots. For the variable length of the longest root, coconut coir: earthworm castings (2:1) and coconut coir : earthworm castings : sand (2:1:1 and 2:2:1) had the highest averages, and for dried root mass, the coconut coir : earthworm castings : sand (1:1:1 and 2:2:1) provided the best results. Germination can be carried out in vitro in a medium containing only water and agar, and for acclimatization, the substrate coconut coir : earthworm castings : sand (2:2:1) should be used._________________________________________________________________________________________ RESUMO: A macambira é uma planta ornamental, que apresenta elevado grau de importância ecológica nos ecossistemas. O objetivo deste trabalho foi avaliar a germinação in vitro da macambira em função de diferentes meios de cultura, além de estabelecer o melhor substrato para a aclimatização de mudas micropropagadas. Para tanto, foram testados cinco tratamentos (DIC), com 100, 75, 50, 25 e 0% dos sais do meio MS. Foi estimada a porcentagem de germinação e verificado o índice de velocidade de germinação (IVG). Para aclimatização, foram avaliadas cinco combinações de substratos: pó de coco : húmus de minhoca (2:1); pó de coco : húmus de minhoca : areia (1:1:1), (2:1:1), (2:2:1), e vermiculita: húmus de minhoca (2:1). Não houve diferença entre as concentrações de sais MS quanto à porcentagem de germinação. Quanto ao IVG, o tratamento 100% de sais MS apresentou o pior resultado. Na aclimatização das mudas, para as variáveis ‘número de folhas e de raízes’ e ‘matéria seca de parte aérea’, não houve diferença. Para a variável ‘comprimento da maior raiz’, os substratos pó de coco : húmus de minhoca (2:1) e pó de coco : húmus de minhoca : areia (2:1:1) e (2:2:1) apresentaram as maiores médias e, para matéria seca de raízes, os substratos pó de coco : húmus de minhoca : areia (1:1:1) e (2:2:1) proporcionaram os melhores resultados. A germinação in vitro pode ser realizada em meio contendo apenas água e ágar; para a aclimatização, deve-se utilizar o substrato pó de coco : húmus de minhoca : areia (2:2:1).
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spelling Arrigoni-Blank, Maria de FátimaSantos, Franciane dosOliveira, Ana Catarina Lima deBlank, Arie FitzgeraldSantos, Mariana de SouzaMenezes, Thays Saynara Alves2016-01-14T16:59:41Z2016-01-14T16:59:41Z2013ARRIGONI-BLANK, M. F. et al. In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings. Revista de Ciências Agrárias, Belém, v. 56, 2013. Suplemento. Disponível em: <https://periodicos.ufra.edu.br/index.php?journal=ajaes&page=article&op=view&path%5B%5D=1433&path%5B%5D=453>. Acesso em: 14 jan. 2016.2177-8760https://ri.ufs.br/handle/riufs/1586Licença Creative CommonsThe macambira is an ornamental plant that has a high degree of ecological importance. The objective in this research was to evaluate the in vitro germination of macambira in different culture media and establish the best substrate for the acclimatization of micropropagated plants. Five treatments were tested: 0, 25, 50, 75, and 100% salt of Murashige and Skoog (MS) medium. We calculated the percentage of germination and germination speed index (GSI). For testing acclimatization, five combinations of substrates were evaluated: coconut coir : earthworm castings (2:1); coconut coir : earthworm castings: sand (1:1:1, 2:1:1 and 2:2:1); and vermiculite : earthworm castings (2:1). There was no difference between the concentrations of MS salts based on the percentage of germination. For the GSI, the treatment 100% MS salts had the poorest results. There was no significant difference in the acclimatization of seedlings for the variables number of leaves and dried mass of roots and shoots. For the variable length of the longest root, coconut coir: earthworm castings (2:1) and coconut coir : earthworm castings : sand (2:1:1 and 2:2:1) had the highest averages, and for dried root mass, the coconut coir : earthworm castings : sand (1:1:1 and 2:2:1) provided the best results. Germination can be carried out in vitro in a medium containing only water and agar, and for acclimatization, the substrate coconut coir : earthworm castings : sand (2:2:1) should be used._________________________________________________________________________________________ RESUMO: A macambira é uma planta ornamental, que apresenta elevado grau de importância ecológica nos ecossistemas. O objetivo deste trabalho foi avaliar a germinação in vitro da macambira em função de diferentes meios de cultura, além de estabelecer o melhor substrato para a aclimatização de mudas micropropagadas. Para tanto, foram testados cinco tratamentos (DIC), com 100, 75, 50, 25 e 0% dos sais do meio MS. Foi estimada a porcentagem de germinação e verificado o índice de velocidade de germinação (IVG). Para aclimatização, foram avaliadas cinco combinações de substratos: pó de coco : húmus de minhoca (2:1); pó de coco : húmus de minhoca : areia (1:1:1), (2:1:1), (2:2:1), e vermiculita: húmus de minhoca (2:1). Não houve diferença entre as concentrações de sais MS quanto à porcentagem de germinação. Quanto ao IVG, o tratamento 100% de sais MS apresentou o pior resultado. Na aclimatização das mudas, para as variáveis ‘número de folhas e de raízes’ e ‘matéria seca de parte aérea’, não houve diferença. Para a variável ‘comprimento da maior raiz’, os substratos pó de coco : húmus de minhoca (2:1) e pó de coco : húmus de minhoca : areia (2:1:1) e (2:2:1) apresentaram as maiores médias e, para matéria seca de raízes, os substratos pó de coco : húmus de minhoca : areia (1:1:1) e (2:2:1) proporcionaram os melhores resultados. A germinação in vitro pode ser realizada em meio contendo apenas água e ágar; para a aclimatização, deve-se utilizar o substrato pó de coco : húmus de minhoca : areia (2:2:1).Universidade Federal Rural da AmazôniaBromeliaceaeMS MediumSubstrateMeio MSSubstratoBroméliasMacambiraIn vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlingsGerminação in vitro de sementes e aclimatização de mudas de Macambira (Bromelia laciniosa Martius ex Schultes f.)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILGerminationSeedsMacambira.pdf.jpgGerminationSeedsMacambira.pdf.jpgGenerated Thumbnailimage/jpeg1623https://ri.ufs.br/jspui/bitstream/riufs/1586/4/GerminationSeedsMacambira.pdf.jpg3a09dad6244b0114e562b3d20108e195MD54TEXTGerminationSeedsMacambira.pdf.txtGerminationSeedsMacambira.pdf.txtExtracted texttext/plain18131https://ri.ufs.br/jspui/bitstream/riufs/1586/3/GerminationSeedsMacambira.pdf.txt2b17162d205a87916619f8cdfacafaceMD53ORIGINALGerminationSeedsMacambira.pdfGerminationSeedsMacambira.pdfapplication/pdf765773https://ri.ufs.br/jspui/bitstream/riufs/1586/1/GerminationSeedsMacambira.pdf0b52464f9fb8b281cb92dab7bbae0adcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/1586/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52riufs/15862016-02-01 19:35:08.443oai:ufs.br:riufs/1586Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2016-02-01T22:35:08Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
dc.title.alternative.pt_BR.fl_str_mv Germinação in vitro de sementes e aclimatização de mudas de Macambira (Bromelia laciniosa Martius ex Schultes f.)
title In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
spellingShingle In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
Arrigoni-Blank, Maria de Fátima
Bromeliaceae
MS Medium
Substrate
Meio MS
Substrato
Bromélias
Macambira
title_short In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
title_full In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
title_fullStr In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
title_full_unstemmed In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
title_sort In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings
author Arrigoni-Blank, Maria de Fátima
author_facet Arrigoni-Blank, Maria de Fátima
Santos, Franciane dos
Oliveira, Ana Catarina Lima de
Blank, Arie Fitzgerald
Santos, Mariana de Souza
Menezes, Thays Saynara Alves
author_role author
author2 Santos, Franciane dos
Oliveira, Ana Catarina Lima de
Blank, Arie Fitzgerald
Santos, Mariana de Souza
Menezes, Thays Saynara Alves
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Arrigoni-Blank, Maria de Fátima
Santos, Franciane dos
Oliveira, Ana Catarina Lima de
Blank, Arie Fitzgerald
Santos, Mariana de Souza
Menezes, Thays Saynara Alves
dc.subject.por.fl_str_mv Bromeliaceae
MS Medium
Substrate
Meio MS
Substrato
Bromélias
Macambira
topic Bromeliaceae
MS Medium
Substrate
Meio MS
Substrato
Bromélias
Macambira
description The macambira is an ornamental plant that has a high degree of ecological importance. The objective in this research was to evaluate the in vitro germination of macambira in different culture media and establish the best substrate for the acclimatization of micropropagated plants. Five treatments were tested: 0, 25, 50, 75, and 100% salt of Murashige and Skoog (MS) medium. We calculated the percentage of germination and germination speed index (GSI). For testing acclimatization, five combinations of substrates were evaluated: coconut coir : earthworm castings (2:1); coconut coir : earthworm castings: sand (1:1:1, 2:1:1 and 2:2:1); and vermiculite : earthworm castings (2:1). There was no difference between the concentrations of MS salts based on the percentage of germination. For the GSI, the treatment 100% MS salts had the poorest results. There was no significant difference in the acclimatization of seedlings for the variables number of leaves and dried mass of roots and shoots. For the variable length of the longest root, coconut coir: earthworm castings (2:1) and coconut coir : earthworm castings : sand (2:1:1 and 2:2:1) had the highest averages, and for dried root mass, the coconut coir : earthworm castings : sand (1:1:1 and 2:2:1) provided the best results. Germination can be carried out in vitro in a medium containing only water and agar, and for acclimatization, the substrate coconut coir : earthworm castings : sand (2:2:1) should be used._________________________________________________________________________________________ RESUMO: A macambira é uma planta ornamental, que apresenta elevado grau de importância ecológica nos ecossistemas. O objetivo deste trabalho foi avaliar a germinação in vitro da macambira em função de diferentes meios de cultura, além de estabelecer o melhor substrato para a aclimatização de mudas micropropagadas. Para tanto, foram testados cinco tratamentos (DIC), com 100, 75, 50, 25 e 0% dos sais do meio MS. Foi estimada a porcentagem de germinação e verificado o índice de velocidade de germinação (IVG). Para aclimatização, foram avaliadas cinco combinações de substratos: pó de coco : húmus de minhoca (2:1); pó de coco : húmus de minhoca : areia (1:1:1), (2:1:1), (2:2:1), e vermiculita: húmus de minhoca (2:1). Não houve diferença entre as concentrações de sais MS quanto à porcentagem de germinação. Quanto ao IVG, o tratamento 100% de sais MS apresentou o pior resultado. Na aclimatização das mudas, para as variáveis ‘número de folhas e de raízes’ e ‘matéria seca de parte aérea’, não houve diferença. Para a variável ‘comprimento da maior raiz’, os substratos pó de coco : húmus de minhoca (2:1) e pó de coco : húmus de minhoca : areia (2:1:1) e (2:2:1) apresentaram as maiores médias e, para matéria seca de raízes, os substratos pó de coco : húmus de minhoca : areia (1:1:1) e (2:2:1) proporcionaram os melhores resultados. A germinação in vitro pode ser realizada em meio contendo apenas água e ágar; para a aclimatização, deve-se utilizar o substrato pó de coco : húmus de minhoca : areia (2:2:1).
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-01-14T16:59:41Z
dc.date.available.fl_str_mv 2016-01-14T16:59:41Z
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dc.identifier.citation.fl_str_mv ARRIGONI-BLANK, M. F. et al. In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings. Revista de Ciências Agrárias, Belém, v. 56, 2013. Suplemento. Disponível em: <https://periodicos.ufra.edu.br/index.php?journal=ajaes&page=article&op=view&path%5B%5D=1433&path%5B%5D=453>. Acesso em: 14 jan. 2016.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/handle/riufs/1586
dc.identifier.issn.none.fl_str_mv 2177-8760
dc.identifier.license.pt_BR.fl_str_mv Licença Creative Commons
identifier_str_mv ARRIGONI-BLANK, M. F. et al. In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings. Revista de Ciências Agrárias, Belém, v. 56, 2013. Suplemento. Disponível em: <https://periodicos.ufra.edu.br/index.php?journal=ajaes&page=article&op=view&path%5B%5D=1433&path%5B%5D=453>. Acesso em: 14 jan. 2016.
2177-8760
Licença Creative Commons
url https://ri.ufs.br/handle/riufs/1586
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