Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos

Detalhes bibliográficos
Autor(a) principal: Correia, Hugo José de Araújo
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: http://ri.ufs.br/jspui/handle/riufs/16200
Resumo: Kefir is a complex food, as its microbiological composition aggregates a large number of symbiotic microrganisms, of which several bacteria have been identified as probiotic. However, its strong aroma and sour taste are unappreciated by most people. So, a good alternative to improve the consumption of this food is microencapsulation. A technique in which microdrops of an active ingredient are surrounded by a homogeneous or heterogeneous coating, congruent to capsules with many applications, so that it is protected against external factors, masking unpleasant aromas, and allowing the development of products with high added value. One of the viable microencapsulation techniques is the ionic gelation method, that is, a reaction between a polymeric solution and an ionic solution. Given the above, the objective of this study was to develop microcapsules of kefir, to be consumed individually or with other foods; and analyze the stability of probiotics, as well as the physical, physicochemical and microbiological changes in the product (kefir) and in the microcapsules made from it, during refrigerated storage at 5±1 °C for 28 days in transparent glass packaging. Analyzes were performed in triplicate and Tukey's test (p < 0.05) was applied for comparisons of means, with the results expressed as mean ± standard deviation. During storage, there was a product with microorganism counts within the standards for lactic acid bacteria around 107CFU/mL and yeast 104 CFU/mL at the end of 28 days of storage. It was observed that there was no significant difference (p > 0.05) in the number of lactic acid bacteria between kefir and microcapsules during the storage period; but there was in the number of lactic acid bacteria in kefir in the 28 days. So, microencapsulation of kefir was effective in protecting lactic acid bacteria from adverse environmental conditions. The pH of kefir and the pH of the microcapsules showed a significant difference between them in the initial and final time. The initial titratable acidity of kefir and microcapsules was 1.05% and 0.66%, respectively. As kefir has a high acidity, its fresh consumption is not well accepted. So, the reduction of acidity in the microcapsules added value to them, as they can be consumed as a more palatable product. Thus, obtaining kefir microcapsules had positive points due to the formation of a product with a high nutritional and functional value, which can be applied in the formulation of innovative foods, helping to prevent many diseases and thus potentially benefiting the health of the consumers.
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spelling Correia, Hugo José de AraújoPagani, Alessandra Almeida CastroSantos, Poliane Lima2022-08-24T00:36:09Z2022-08-24T00:36:09Z2022-01-31CORREIA, Hugo José de Araújo. Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos. 2022. 56 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2022.http://ri.ufs.br/jspui/handle/riufs/16200Kefir is a complex food, as its microbiological composition aggregates a large number of symbiotic microrganisms, of which several bacteria have been identified as probiotic. However, its strong aroma and sour taste are unappreciated by most people. So, a good alternative to improve the consumption of this food is microencapsulation. A technique in which microdrops of an active ingredient are surrounded by a homogeneous or heterogeneous coating, congruent to capsules with many applications, so that it is protected against external factors, masking unpleasant aromas, and allowing the development of products with high added value. One of the viable microencapsulation techniques is the ionic gelation method, that is, a reaction between a polymeric solution and an ionic solution. Given the above, the objective of this study was to develop microcapsules of kefir, to be consumed individually or with other foods; and analyze the stability of probiotics, as well as the physical, physicochemical and microbiological changes in the product (kefir) and in the microcapsules made from it, during refrigerated storage at 5±1 °C for 28 days in transparent glass packaging. Analyzes were performed in triplicate and Tukey's test (p < 0.05) was applied for comparisons of means, with the results expressed as mean ± standard deviation. During storage, there was a product with microorganism counts within the standards for lactic acid bacteria around 107CFU/mL and yeast 104 CFU/mL at the end of 28 days of storage. It was observed that there was no significant difference (p > 0.05) in the number of lactic acid bacteria between kefir and microcapsules during the storage period; but there was in the number of lactic acid bacteria in kefir in the 28 days. So, microencapsulation of kefir was effective in protecting lactic acid bacteria from adverse environmental conditions. The pH of kefir and the pH of the microcapsules showed a significant difference between them in the initial and final time. The initial titratable acidity of kefir and microcapsules was 1.05% and 0.66%, respectively. As kefir has a high acidity, its fresh consumption is not well accepted. So, the reduction of acidity in the microcapsules added value to them, as they can be consumed as a more palatable product. Thus, obtaining kefir microcapsules had positive points due to the formation of a product with a high nutritional and functional value, which can be applied in the formulation of innovative foods, helping to prevent many diseases and thus potentially benefiting the health of the consumers.O kefir é um alimento complexo, pois a sua composição microbiológica agrega um grande número de microrganismos simbióticos, dos quais várias bactérias têm sido identificadas como probióticas. Entretanto, seu aroma marcante e sabor ácido são pouco apreciados pela maioria das pessoas, de modo que, uma boa alternativa para melhorar o consumo desse alimento, é a microencapsulação. Nessa técnica, microgotas de um ingrediente ativo são envolvidas por um revestimento homogêneo ou heterogêneo, congruente a cápsulas com muitas aplicações, para que seja protegido contra fatores externos, mascarando aromas desagradáveis, e permitindo o desenvolvimento de produtos de alto valor agregado. Uma das técnicas viáveis de microencapsulação é pelo método de gelificação iônica, ou seja, uma reação entre uma solução polimérica e uma solução iônica. Diante do exposto, o objetivo deste estudo foi desenvolver microcápsulas de kefir, para serem consumidas individualmente ou com outros alimentos; e analisar a estabilidade dos probióticos, bem como as alterações físicas, físico-químicas e microbiológicas do produto (kefir) e das microcápsulas feitas dele, durante o armazenamento refrigerado a 5±1°C por 28 dias em embalagens de vidro transparentes. As análises foram realizadas em triplicata e o teste de Tukey (p < 0,05) foi aplicado para as comparações das médias, sendo os resultados expressos como média ± desvio padrão. Durante o armazenamento, verificou-se um produto com contagens de microrganismos dentro dos padrões para bactérias ácido láticas em torno de 107 UFC/mL e leveduras 104 UFC/mL ao final de 28 dias de estocagem. Observou-se que não houve diferença significativa (p  > 0,05) no número de bactérias láticas entre o kefir e as microcápsulas no período de armazenamento; mas houve no número de bactérias láticas no kefir nos 28 dias. Então, a microencapsulação do kefir foi eficaz em proteger as bactérias ácido láticas das condições adversas do meio em que se encontram. O pH do kefir e o pH das microcápsulas mostraram diferença significativa entre si no tempo inicial e no tempo final. A acidez titulável inicial do kefir e das microcápsulas foi 1,05% e 0,66%, respectivamente. Como o kefir tem uma acidez elevada, o seu consumo in natura não é bem aceito, a redução da acidez nas microcápsulas agregou valor a elas, pois podem ser consumidas como um produto mais palatável. Assim, a obtenção de microcápsulas de kefir apresentou pontos positivos devido à formação de um produto com um alto valor nutricional e funcional, que pode ser aplicado na formulação de alimentos inovadores, auxiliando na prevenção de muitas doenças e com isso, beneficiando potencialmente a saúde dos consumidores.São CristóvãoporKefirProbióticosCompostos bioativosGelificação iônicaProbioticsBioactive compoundsIonic gelationCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSMicroencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentosMicroencapsulation of kefir by the ionic gelation technique for food applicationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em Ciência e Tecnologia de AlimentosUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/16200/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALHUGO_JOSE_ARAUJO_CORREIA.pdfHUGO_JOSE_ARAUJO_CORREIA.pdfapplication/pdf1333818https://ri.ufs.br/jspui/bitstream/riufs/16200/2/HUGO_JOSE_ARAUJO_CORREIA.pdf82bcfdaa8251badd085a96faf3d274acMD52TEXTHUGO_JOSE_ARAUJO_CORREIA.pdf.txtHUGO_JOSE_ARAUJO_CORREIA.pdf.txtExtracted texttext/plain115812https://ri.ufs.br/jspui/bitstream/riufs/16200/3/HUGO_JOSE_ARAUJO_CORREIA.pdf.txtd0d5892838d2eeafa1e174b4e9a31f63MD53THUMBNAILHUGO_JOSE_ARAUJO_CORREIA.pdf.jpgHUGO_JOSE_ARAUJO_CORREIA.pdf.jpgGenerated Thumbnailimage/jpeg1341https://ri.ufs.br/jspui/bitstream/riufs/16200/4/HUGO_JOSE_ARAUJO_CORREIA.pdf.jpg77dbc35db4e2891a8ccd18f4450d1b3cMD54riufs/162002022-08-23 21:36:09.374oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2022-08-24T00:36:09Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos
dc.title.alternative.por.fl_str_mv Microencapsulation of kefir by the ionic gelation technique for food application
title Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos
spellingShingle Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos
Correia, Hugo José de Araújo
Kefir
Probióticos
Compostos bioativos
Gelificação iônica
Probiotics
Bioactive compounds
Ionic gelation
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
title_short Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos
title_full Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos
title_fullStr Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos
title_full_unstemmed Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos
title_sort Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos
author Correia, Hugo José de Araújo
author_facet Correia, Hugo José de Araújo
author_role author
dc.contributor.author.fl_str_mv Correia, Hugo José de Araújo
dc.contributor.advisor1.fl_str_mv Pagani, Alessandra Almeida Castro
dc.contributor.advisor-co1.fl_str_mv Santos, Poliane Lima
contributor_str_mv Pagani, Alessandra Almeida Castro
Santos, Poliane Lima
dc.subject.por.fl_str_mv Kefir
Probióticos
Compostos bioativos
Gelificação iônica
topic Kefir
Probióticos
Compostos bioativos
Gelificação iônica
Probiotics
Bioactive compounds
Ionic gelation
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
dc.subject.eng.fl_str_mv Probiotics
Bioactive compounds
Ionic gelation
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
description Kefir is a complex food, as its microbiological composition aggregates a large number of symbiotic microrganisms, of which several bacteria have been identified as probiotic. However, its strong aroma and sour taste are unappreciated by most people. So, a good alternative to improve the consumption of this food is microencapsulation. A technique in which microdrops of an active ingredient are surrounded by a homogeneous or heterogeneous coating, congruent to capsules with many applications, so that it is protected against external factors, masking unpleasant aromas, and allowing the development of products with high added value. One of the viable microencapsulation techniques is the ionic gelation method, that is, a reaction between a polymeric solution and an ionic solution. Given the above, the objective of this study was to develop microcapsules of kefir, to be consumed individually or with other foods; and analyze the stability of probiotics, as well as the physical, physicochemical and microbiological changes in the product (kefir) and in the microcapsules made from it, during refrigerated storage at 5±1 °C for 28 days in transparent glass packaging. Analyzes were performed in triplicate and Tukey's test (p < 0.05) was applied for comparisons of means, with the results expressed as mean ± standard deviation. During storage, there was a product with microorganism counts within the standards for lactic acid bacteria around 107CFU/mL and yeast 104 CFU/mL at the end of 28 days of storage. It was observed that there was no significant difference (p > 0.05) in the number of lactic acid bacteria between kefir and microcapsules during the storage period; but there was in the number of lactic acid bacteria in kefir in the 28 days. So, microencapsulation of kefir was effective in protecting lactic acid bacteria from adverse environmental conditions. The pH of kefir and the pH of the microcapsules showed a significant difference between them in the initial and final time. The initial titratable acidity of kefir and microcapsules was 1.05% and 0.66%, respectively. As kefir has a high acidity, its fresh consumption is not well accepted. So, the reduction of acidity in the microcapsules added value to them, as they can be consumed as a more palatable product. Thus, obtaining kefir microcapsules had positive points due to the formation of a product with a high nutritional and functional value, which can be applied in the formulation of innovative foods, helping to prevent many diseases and thus potentially benefiting the health of the consumers.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-08-24T00:36:09Z
dc.date.available.fl_str_mv 2022-08-24T00:36:09Z
dc.date.issued.fl_str_mv 2022-01-31
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dc.identifier.citation.fl_str_mv CORREIA, Hugo José de Araújo. Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos. 2022. 56 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2022.
dc.identifier.uri.fl_str_mv http://ri.ufs.br/jspui/handle/riufs/16200
identifier_str_mv CORREIA, Hugo José de Araújo. Microencapsulação de kefir pela técnica de gelificação iônica para aplicação em alimentos. 2022. 56 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2022.
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