Nanopartículas lipídicas contendo pentamidina para tratamento da leishmaniose tegumentar : obtenção e avaliação da liberação In Vitro

• Autor(a) principal: Silva, Hoany Machado da
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/10586
• topic: Farmácia; Ensino de farmácia; Pentamidina; Leishmaniose tegumentar; Leishmania; In Vitro; Leshmaniose cutânea; Pentamidine; Cutaneous Leishmaniasis; Nanostructured Lipid Carriers; CIENCIAS DA SAUDE::FARMACIA

Pentamidine (PTD) is a drug used in the treatment of cutaneous leishmaniasis. It has high 55 toxicity and is administered only by parenteral route, for intramuscular injection. In order to 56 overcome these limitations, one option is to incorporate PTD in delivery systems, such as 57 nanostructured lipid carriers (CLN). The objective of this work was to incorporate PTD in CLN, 58 characterize the obtained systems and evaluate its in vitro release. Nanoparticles were obtained by 59 the method of multiple emulsion (w / o / w) associated with ultrasonication. The particle sizes, 60 polydispersity index (IPD), zeta potential and encapsulation efficiency were determined. And the 61 release studies were performed in Franz type diffusion cells using as the hydrophilic barrier 62 membrane of cellulose acetate. As a result, the obtained particles had a size range between 133 and 63 266 nm, IPD between 0.5 to 0.7, the zeta potential ranging between -10 to -26 mV and the 64 encapsulation efficiency of PTD was 76%. The release studies presented a sustained release profile, 65 releasing 36% of drug in 24 hour experiment. According to the results presented, CLN containing 66 PTD were obtained successfully suggesting further studies to evaluate the leishmanicide drug effect 67 with reduced toxicity.