Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Ana Roseli Silva
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/3998
Resumo: Caesalpinia pyramidalis Tul. (Fabaceae), known as catingueira , have been used in folk medicine for the treatment of various disorders such as gastritis, heartburn, indigestion, and stomach ache. However, the gastroprotective properties of this species have not yet been studied. The ethanol extract of C. pyramidalis inner bark was used in rats via oral, at the doses of 30, 100, and 300 mg/kg. The antiulcer assays were performed using the ethanol- and nonsteroidal anti-inflammatory drug-induced ulcer protocols. Gastric secretion parameters (volume, pH, and total acidity) were also evaluated by the pylorus ligated model, and the mucus in the gastric content was determined. The anti-Helicobacter pylori activity of the ethanol extract of Caesalpinia pyramidalis was performed using the agar-well diffusion and broth microdilution methods to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values. The ethanol extract (30, 100, and 300 mg/kg) produced dose dependent inhibition (p < 0.01) on the ulcer lesion index in the ethanol-induced ulcer model. The ethanol extract (30, 100, and 300 mg/kg) also reduced (p < 0.001) the ulcer index in the indomethacin-induced ulcer model. In the model ligature pylorus, the treatment with C. pyramidalis ethanol extract failed to significantly change the gastric secretion parameters. However, after treatment with the ethanol extract of C. pyramidalis (30, 100, and 300 mg/kg), there was a significant increase (p < 0.05) in mucus production. The ethanol extract of Caesalpinia pyramidalis had an anti-Helicobacter pylori activity with a MIC of 625 ìg/mL and a MBC of 10.000 ìg/mL. Collectively, the present results suggest that the ethanol extract of C. pyramidalis display gastroprotective actions, supporting the folkloric usage of the plant to treat various gastrointestinal disturbances.
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spelling Ribeiro, Ana Roseli Silvahttp://lattes.cnpq.br/9879983198868216Thomazzi, Sara Mariahttp://lattes.cnpq.br/60048075542771592017-09-26T12:31:22Z2017-09-26T12:31:22Z2013-03-06https://ri.ufs.br/handle/riufs/3998Caesalpinia pyramidalis Tul. (Fabaceae), known as catingueira , have been used in folk medicine for the treatment of various disorders such as gastritis, heartburn, indigestion, and stomach ache. However, the gastroprotective properties of this species have not yet been studied. The ethanol extract of C. pyramidalis inner bark was used in rats via oral, at the doses of 30, 100, and 300 mg/kg. The antiulcer assays were performed using the ethanol- and nonsteroidal anti-inflammatory drug-induced ulcer protocols. Gastric secretion parameters (volume, pH, and total acidity) were also evaluated by the pylorus ligated model, and the mucus in the gastric content was determined. The anti-Helicobacter pylori activity of the ethanol extract of Caesalpinia pyramidalis was performed using the agar-well diffusion and broth microdilution methods to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values. The ethanol extract (30, 100, and 300 mg/kg) produced dose dependent inhibition (p < 0.01) on the ulcer lesion index in the ethanol-induced ulcer model. The ethanol extract (30, 100, and 300 mg/kg) also reduced (p < 0.001) the ulcer index in the indomethacin-induced ulcer model. In the model ligature pylorus, the treatment with C. pyramidalis ethanol extract failed to significantly change the gastric secretion parameters. However, after treatment with the ethanol extract of C. pyramidalis (30, 100, and 300 mg/kg), there was a significant increase (p < 0.05) in mucus production. The ethanol extract of Caesalpinia pyramidalis had an anti-Helicobacter pylori activity with a MIC of 625 ìg/mL and a MBC of 10.000 ìg/mL. Collectively, the present results suggest that the ethanol extract of C. pyramidalis display gastroprotective actions, supporting the folkloric usage of the plant to treat various gastrointestinal disturbances.Caesalpinia pyramidalis Tul. (Fabaceae), conhecida popularmente como gcatingueira h, tem sido usada pela medicina popular para tratamento de diversas desordens fisiologicas como gastrite, indigestao e dores estomacais. Entretanto, a acao gastroprotetora dessa especie ainda nao foi estudada. O extrato etanolico da entrecasca (EEE) da Caesalpinia pyramidalis foi administrado em ratos pela via oral, nas doses de 30, 100 e 300 mg/kg. Para avaliacao da atividade gastroprotetora foram utilizados os modelos de ulceras agudas induzidas por etanol e anti-inflamatorio nao esteroidal (AINE). Parametros da secrecao gastrica (volume, pH e acidez total) e producao de muco gastrico tambem foram avaliados pela tecnica de ligadura de piloro. A atividade antimicrobiana do EEE da Caesalpinia pyramidalis foi mensurada atraves da tecnica de difusao em agar e calculados os valores da concentracao inibitoria minima (CIM) e da concentracao bactericida minima (CBM) pela tecnica de microdiluicao em placa. O EEE da Caesalpinia pyramidalis nas doses de 30, 100 e 300 mg/kg inibiu de maneira dose dependente (p < 0,01) o indice de lesoes ulcerativas no modelo de ulceras induzidas por etanol. No modelo de ulceras induzidas por AINE, o extrato em todas as doses utilizadas, tambem reduziu o indice de lesoes ulcerativas (p < 0,001). No modelo de ligadura de piloro, o tratamento com o EEE da Caesalpinia pyramidalis nao foi capaz de modificar os parametros da secrecao gastrica. Entretanto, apos tratamento com o EEE da Caesalpinia pyramidalis (30, 100 e 300 mg/kg) observou-se um aumento significativo (p < 0,05) na producao do muco gastrico. O EEE da Caesalpinia pyramidalis possui atividade antimicrobiana contra o Helicobacter pylori com CIM de 625 Êg/mL e CBM de 10.000 Êg/mL. Os resultados deste estudo sugerem que o EEE da Caesalpinia pyramidalis possui acao gastroprotetora, desta forma validando a utilizacao popular desta planta no tratamento de disturbios gastrointestinais.application/pdfporLeguminosaPlantas da caatingaCaesalpina pyramidalisHelicobacter pyloriUlcera gastricaCaatinga plantsLegumesCNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIAEfeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em Ciências Fisiológicasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSORIGINALANA_ROSELI_SILVA_RIBEIRO.pdfapplication/pdf776704https://ri.ufs.br/jspui/bitstream/riufs/3998/1/ANA_ROSELI_SILVA_RIBEIRO.pdf3bf1be8732234aeabab7bbb52d3fa194MD51TEXTANA_ROSELI_SILVA_RIBEIRO.pdf.txtANA_ROSELI_SILVA_RIBEIRO.pdf.txtExtracted texttext/plain100456https://ri.ufs.br/jspui/bitstream/riufs/3998/2/ANA_ROSELI_SILVA_RIBEIRO.pdf.txt86c9b723c58b57a10157dd2b2fcaf036MD52THUMBNAILANA_ROSELI_SILVA_RIBEIRO.pdf.jpgANA_ROSELI_SILVA_RIBEIRO.pdf.jpgGenerated Thumbnailimage/jpeg1293https://ri.ufs.br/jspui/bitstream/riufs/3998/3/ANA_ROSELI_SILVA_RIBEIRO.pdf.jpge925370d1e358b6029913eb5ee851083MD53riufs/39982017-11-24 21:45:48.127oai:ufs.br:riufs/3998Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-25T00:45:48Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.por.fl_str_mv Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos
title Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos
spellingShingle Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos
Ribeiro, Ana Roseli Silva
Leguminosa
Plantas da caatinga
Caesalpina pyramidalis
Helicobacter pylori
Ulcera gastrica
Caatinga plants
Legumes
CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA
title_short Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos
title_full Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos
title_fullStr Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos
title_full_unstemmed Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos
title_sort Efeito gastropotetor do extrato etanólico da entrecasca da Caesalpina pyramidalis Tul. em ratos
author Ribeiro, Ana Roseli Silva
author_facet Ribeiro, Ana Roseli Silva
author_role author
dc.contributor.author.fl_str_mv Ribeiro, Ana Roseli Silva
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9879983198868216
dc.contributor.advisor1.fl_str_mv Thomazzi, Sara Maria
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6004807554277159
contributor_str_mv Thomazzi, Sara Maria
dc.subject.por.fl_str_mv Leguminosa
Plantas da caatinga
Caesalpina pyramidalis
Helicobacter pylori
Ulcera gastrica
topic Leguminosa
Plantas da caatinga
Caesalpina pyramidalis
Helicobacter pylori
Ulcera gastrica
Caatinga plants
Legumes
CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA
dc.subject.eng.fl_str_mv Caatinga plants
Legumes
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA
description Caesalpinia pyramidalis Tul. (Fabaceae), known as catingueira , have been used in folk medicine for the treatment of various disorders such as gastritis, heartburn, indigestion, and stomach ache. However, the gastroprotective properties of this species have not yet been studied. The ethanol extract of C. pyramidalis inner bark was used in rats via oral, at the doses of 30, 100, and 300 mg/kg. The antiulcer assays were performed using the ethanol- and nonsteroidal anti-inflammatory drug-induced ulcer protocols. Gastric secretion parameters (volume, pH, and total acidity) were also evaluated by the pylorus ligated model, and the mucus in the gastric content was determined. The anti-Helicobacter pylori activity of the ethanol extract of Caesalpinia pyramidalis was performed using the agar-well diffusion and broth microdilution methods to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values. The ethanol extract (30, 100, and 300 mg/kg) produced dose dependent inhibition (p < 0.01) on the ulcer lesion index in the ethanol-induced ulcer model. The ethanol extract (30, 100, and 300 mg/kg) also reduced (p < 0.001) the ulcer index in the indomethacin-induced ulcer model. In the model ligature pylorus, the treatment with C. pyramidalis ethanol extract failed to significantly change the gastric secretion parameters. However, after treatment with the ethanol extract of C. pyramidalis (30, 100, and 300 mg/kg), there was a significant increase (p < 0.05) in mucus production. The ethanol extract of Caesalpinia pyramidalis had an anti-Helicobacter pylori activity with a MIC of 625 ìg/mL and a MBC of 10.000 ìg/mL. Collectively, the present results suggest that the ethanol extract of C. pyramidalis display gastroprotective actions, supporting the folkloric usage of the plant to treat various gastrointestinal disturbances.
publishDate 2013
dc.date.issued.fl_str_mv 2013-03-06
dc.date.accessioned.fl_str_mv 2017-09-26T12:31:22Z
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