Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p23 |
Resumo: | Penicillium expansum is a cosmopolitan, highly aggressive pathogen that causes blue mold, a disease of great importance that leads to losses in quality and quantity of harvested fruits. The application of chemicals is traditionally used as a control method. However, algae bioprospecting has revealed many antifungal compounds that can be used to control pathogens. Thus, the objective of this study was to evaluate the effects of ethanolic extracts from seven microalgae and five macroalgae against P. expansum. The antifungal potential was evaluated by analyzing germination percentage, the size of the germ tube, minimum inhibitory concentration (MIC), and the median effective concentration (EC50). The spectrophotometric profile was determined for extracts that showed an inhibitory effect. Among the investigated algae, the Chlorella sp. and H. pluvialis extracts, which had final concentrations of 18.8 and 125.95mg.mL-1, inhibited 100% and 91% germination, respectively. The EC50 was 2.93 and 61.20 mg.mL-1 for Chlorella sp. and H. pluvialis, respectively. Chlorella sp. showed absorption peaks in the range of chlorophyll-a and H. pluvialis presented a peak in the range of phenolic compounds. Although further studies are required to characterize the extracts, Chlorella sp. and H. pluvialis showed promising antifungal effects on the control of P. expansum. |
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Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota)Atividade antimicrobiana de extratos etanólicos de algas no controle de Penicillium expansum Link (Trichocomaceae, Ascomycota)Penicillium expansum is a cosmopolitan, highly aggressive pathogen that causes blue mold, a disease of great importance that leads to losses in quality and quantity of harvested fruits. The application of chemicals is traditionally used as a control method. However, algae bioprospecting has revealed many antifungal compounds that can be used to control pathogens. Thus, the objective of this study was to evaluate the effects of ethanolic extracts from seven microalgae and five macroalgae against P. expansum. The antifungal potential was evaluated by analyzing germination percentage, the size of the germ tube, minimum inhibitory concentration (MIC), and the median effective concentration (EC50). The spectrophotometric profile was determined for extracts that showed an inhibitory effect. Among the investigated algae, the Chlorella sp. and H. pluvialis extracts, which had final concentrations of 18.8 and 125.95mg.mL-1, inhibited 100% and 91% germination, respectively. The EC50 was 2.93 and 61.20 mg.mL-1 for Chlorella sp. and H. pluvialis, respectively. Chlorella sp. showed absorption peaks in the range of chlorophyll-a and H. pluvialis presented a peak in the range of phenolic compounds. Although further studies are required to characterize the extracts, Chlorella sp. and H. pluvialis showed promising antifungal effects on the control of P. expansum.Penicillium expansum destaca-se como fitopatógeno cosmopolita e altamente agressivo, responsável pelo bolor azul, doença que proporciona a perda de qualidade e quantidade pós colheita de frutos. Aplicações de produtos químicos é tradicionalmente o método de controle mais utilizado. No entanto, a bioprospecção de algas tem-se revelado uma fonte para uma grande quantidade de compostos antifúngicos. Desta maneira, o presente trabalho tem por objetivo avaliar os efeitos dos extratos etanólicos de sete microalgas e cinco macroalgas contra P. expasum. O potencial antifúngico foi avaliado analisando-se a porcentagem de germinação, o tamanho do tubo germinativo, a concentração mínima inibitória e a concentração efetiva mediana (CE50). O perfil espectrofotométrico foi realizado nos extratos que apresentaram efeito inibitório. Dentre as algas estudadas, os extratos de Chlorella sp. e H. pluvialis, nas concentrações finais de 18,8 e 125,95 mg.mL-1, foram capazes de inibir 100 e 91% da germinação, respectivamente. A CE50 foi de 2,93 e 61,20 mg.mL-1, para Chlorella sp. e H. pluvialis, respectivamente. Chlorella sp. apresentou picos de absorção na faixa da clorofila-a, já H. pluvialis apresentou um pico correspondente a faixa dos compostos fenólicos. Embora sejam necessários mais estudos para caracterizar os extratos, Chlorella sp. e H. pluvialis apresentaram efeito antifúngico promissor no controle de P. expansum. Universidade Federal de Santa Catarina2015-08-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p2310.5007/2175-7925.2015v28n4p23Biotemas; v. 28 n. 4 (2015); 23-332175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p23/30385Copyright (c) 2015 Argus Cezar da Rocha Neto, Luana dos Santos de Souza, Elisangela Angelo, Rafaela Gordo, Eduardo de Oliveira Bastos, Maria Cecília Miotto, José Bonomi Barufi, Marcelo Maraschin, Roberto Derner, Leonardo Röriginfo:eu-repo/semantics/openAccessda Rocha Neto, Argus Cezarde Souza, Luana dos SantosAngelo, ElisangelaGordo, RafaelaBastos, Eduardo de OliveiraMiotto, Maria CecíliaBarufi, José BonomiMaraschin, MarceloDerner, RobertoRörig, Leonardo2017-10-19T15:56:25Zoai:periodicos.ufsc.br:article/38377Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2017-10-19T15:56:25Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota) Atividade antimicrobiana de extratos etanólicos de algas no controle de Penicillium expansum Link (Trichocomaceae, Ascomycota) |
title |
Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota) |
spellingShingle |
Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota) da Rocha Neto, Argus Cezar |
title_short |
Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota) |
title_full |
Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota) |
title_fullStr |
Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota) |
title_full_unstemmed |
Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota) |
title_sort |
Antimicrobial activity of ethanolic extracts from algae against Penicillium expansum Link (Trichocomaceae, Ascomycota) |
author |
da Rocha Neto, Argus Cezar |
author_facet |
da Rocha Neto, Argus Cezar de Souza, Luana dos Santos Angelo, Elisangela Gordo, Rafaela Bastos, Eduardo de Oliveira Miotto, Maria Cecília Barufi, José Bonomi Maraschin, Marcelo Derner, Roberto Rörig, Leonardo |
author_role |
author |
author2 |
de Souza, Luana dos Santos Angelo, Elisangela Gordo, Rafaela Bastos, Eduardo de Oliveira Miotto, Maria Cecília Barufi, José Bonomi Maraschin, Marcelo Derner, Roberto Rörig, Leonardo |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
da Rocha Neto, Argus Cezar de Souza, Luana dos Santos Angelo, Elisangela Gordo, Rafaela Bastos, Eduardo de Oliveira Miotto, Maria Cecília Barufi, José Bonomi Maraschin, Marcelo Derner, Roberto Rörig, Leonardo |
description |
Penicillium expansum is a cosmopolitan, highly aggressive pathogen that causes blue mold, a disease of great importance that leads to losses in quality and quantity of harvested fruits. The application of chemicals is traditionally used as a control method. However, algae bioprospecting has revealed many antifungal compounds that can be used to control pathogens. Thus, the objective of this study was to evaluate the effects of ethanolic extracts from seven microalgae and five macroalgae against P. expansum. The antifungal potential was evaluated by analyzing germination percentage, the size of the germ tube, minimum inhibitory concentration (MIC), and the median effective concentration (EC50). The spectrophotometric profile was determined for extracts that showed an inhibitory effect. Among the investigated algae, the Chlorella sp. and H. pluvialis extracts, which had final concentrations of 18.8 and 125.95mg.mL-1, inhibited 100% and 91% germination, respectively. The EC50 was 2.93 and 61.20 mg.mL-1 for Chlorella sp. and H. pluvialis, respectively. Chlorella sp. showed absorption peaks in the range of chlorophyll-a and H. pluvialis presented a peak in the range of phenolic compounds. Although further studies are required to characterize the extracts, Chlorella sp. and H. pluvialis showed promising antifungal effects on the control of P. expansum. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p23 10.5007/2175-7925.2015v28n4p23 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p23 |
identifier_str_mv |
10.5007/2175-7925.2015v28n4p23 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p23/30385 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 28 n. 4 (2015); 23-33 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940553600663552 |