Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n2p111 |
Resumo: | We performed histologic evaluation of the parotid glands of 10 (male and female) Rhinella schneideri. Animals were manually captured during nocturnal collections in Bom Jesus, Piauí, and euthanized by administering a lethal dose of anesthetic thiopental. The parotid glands were then collected and embedded in paraffin, and sectioned to 4 µm blocks using a manual rotary microtome. Considering the lack of general information concerning anatomical and histological conditions of Rhinella scheneideri, we sought to address key characteristics of macro glands found in the species with the goal of a better understanding of the operation of this type of defense mechanism. Histological analysis revealed the presence of macrogland alveoli with ducts encircled with differentiated mucous cells, known as "accessory glands", as well as granular glands. We also discovered glandular ducts on the glands which communicate with the outside of the body. These ducts are internally lined by duct glandular epithelial cells, forming a plug which promotes total obstruction of the duct. There were no histological differences in macro gland anatomy in this species compared to other species in Bufonidae. |
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Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae)Histologia da glândula parotoide de anuros da espécie Rhinella schneideri (Amphibia: Bufonidae)We performed histologic evaluation of the parotid glands of 10 (male and female) Rhinella schneideri. Animals were manually captured during nocturnal collections in Bom Jesus, Piauí, and euthanized by administering a lethal dose of anesthetic thiopental. The parotid glands were then collected and embedded in paraffin, and sectioned to 4 µm blocks using a manual rotary microtome. Considering the lack of general information concerning anatomical and histological conditions of Rhinella scheneideri, we sought to address key characteristics of macro glands found in the species with the goal of a better understanding of the operation of this type of defense mechanism. Histological analysis revealed the presence of macrogland alveoli with ducts encircled with differentiated mucous cells, known as "accessory glands", as well as granular glands. We also discovered glandular ducts on the glands which communicate with the outside of the body. These ducts are internally lined by duct glandular epithelial cells, forming a plug which promotes total obstruction of the duct. There were no histological differences in macro gland anatomy in this species compared to other species in Bufonidae.O estudo histológico da glândula parotoide foi realizado utilizando 10 exemplares (machos e fêmeas) da espécie Rhinella schneideri, capturados manualmente em coletas noturnas no município de Bom Jesus, Piauí. Posteriormente, foram eutanasiados por administração de uma dose letal de anestésico Tiopental e seguidamente as glândulas parotoides foram coletadas e submetidas a processamento histológico padrão com inclusão em parafina e os blocos seccionados em micrótomo rotativo manual a espessura de 4 ?m. Diante da escassez de informações sobre condições anátomo-histológica de Rhinella scheneideri, buscou-se abordar as principais características marcantes das macroglândulas encontradas na espécie, conhecimento importante para compreensão do funcionamento deste mecanismo de defesa da espécie. Histologicamente, evidenciamos a presença dos alvéolos das macroglândulas, com presença de ductos circundados com células mucosas diferenciadas, as “glândulas acessórias”, sendo também encontradas glândulas granulosas. Verificou-se ainda que cada glândula possui ductos que comunicam o corpo glandular com o meio exterior, sendo o ducto revestido internamente por células epiteliais glandulares do próprio ducto, constituindo um plug, que em Rhinella schneideri promove a obstrução total do ducto. Essas macroglândulas não apresentam diferenciação histológica quando se comparado a outras espécies da família Bufonidae.Universidade Federal de Santa Catarina2015-02-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n2p11110.5007/2175-7925.2015v28n2p111Biotemas; v. 28 n. 2 (2015); 111-1182175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n2p111/29248Copyright (c) 2015 Juliana Costa Sousa, Regina Lucia dos Santos Silva, Richard Átila de Sousa, Mauro Sergio Cruz Souza Lima, Guilherme José Bolzani de Campos Ferreirainfo:eu-repo/semantics/openAccessSousa, Juliana CostaSilva, Regina Lucia dos SantosSousa, Richard Átila deLima, Mauro Sergio Cruz SouzaFerreira, Guilherme José Bolzani de Campos2017-10-19T16:37:20Zoai:periodicos.ufsc.br:article/36064Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2017-10-19T16:37:20Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae) Histologia da glândula parotoide de anuros da espécie Rhinella schneideri (Amphibia: Bufonidae) |
title |
Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae) |
spellingShingle |
Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae) Sousa, Juliana Costa |
title_short |
Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae) |
title_full |
Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae) |
title_fullStr |
Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae) |
title_full_unstemmed |
Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae) |
title_sort |
Histology of parotoid gland of Anuran species Rhinella schneideri (Amphibia: Bufonidae) |
author |
Sousa, Juliana Costa |
author_facet |
Sousa, Juliana Costa Silva, Regina Lucia dos Santos Sousa, Richard Átila de Lima, Mauro Sergio Cruz Souza Ferreira, Guilherme José Bolzani de Campos |
author_role |
author |
author2 |
Silva, Regina Lucia dos Santos Sousa, Richard Átila de Lima, Mauro Sergio Cruz Souza Ferreira, Guilherme José Bolzani de Campos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Sousa, Juliana Costa Silva, Regina Lucia dos Santos Sousa, Richard Átila de Lima, Mauro Sergio Cruz Souza Ferreira, Guilherme José Bolzani de Campos |
description |
We performed histologic evaluation of the parotid glands of 10 (male and female) Rhinella schneideri. Animals were manually captured during nocturnal collections in Bom Jesus, Piauí, and euthanized by administering a lethal dose of anesthetic thiopental. The parotid glands were then collected and embedded in paraffin, and sectioned to 4 µm blocks using a manual rotary microtome. Considering the lack of general information concerning anatomical and histological conditions of Rhinella scheneideri, we sought to address key characteristics of macro glands found in the species with the goal of a better understanding of the operation of this type of defense mechanism. Histological analysis revealed the presence of macrogland alveoli with ducts encircled with differentiated mucous cells, known as "accessory glands", as well as granular glands. We also discovered glandular ducts on the glands which communicate with the outside of the body. These ducts are internally lined by duct glandular epithelial cells, forming a plug which promotes total obstruction of the duct. There were no histological differences in macro gland anatomy in this species compared to other species in Bufonidae. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n2p111 10.5007/2175-7925.2015v28n2p111 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n2p111 |
identifier_str_mv |
10.5007/2175-7925.2015v28n2p111 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n2p111/29248 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 28 n. 2 (2015); 111-118 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940553556623360 |