Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p45 |
Resumo: | This study evaluates the hydroalcoholic extract obtained from the cork cambium of Hymenaea stigonocarpa (jatobá-do-cerrado) on the meristematic cells of Allium cepa roots, at 3 concentrations, 0.5, 1.0, and 1.5 mg/mL, and 2 exposure times, 24 h and 48 h. The slides were made through the crushing technique. Cells were analyzed throughout the cell cycle, totaling 5,000 for each control and concentration group. We found that the 3 concentrations significantly decreased the cell division rate at the exposure time of 48 h when compared to their respective controls. The mitotic index of the exposure time of 48 h was statistically different when compared to the cell division rate of the exposure time of 24 h for all concentrations. The number of cell abnormalities observed was not statistically significant by means of the chi-square test used. Therefore, under the conditions analyzed, the 3 concentrations tested for the cork cambium of jatobá-do-cerrado significantly decreased, in statistical terms, the cell division rate in the meristematic cells of A. cepa roots. |
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Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. rootsEfeito antiproliferativo do extrato hidroalcoólico de Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) sobre as células meristemáticas de raízes de Allium cepa LThis study evaluates the hydroalcoholic extract obtained from the cork cambium of Hymenaea stigonocarpa (jatobá-do-cerrado) on the meristematic cells of Allium cepa roots, at 3 concentrations, 0.5, 1.0, and 1.5 mg/mL, and 2 exposure times, 24 h and 48 h. The slides were made through the crushing technique. Cells were analyzed throughout the cell cycle, totaling 5,000 for each control and concentration group. We found that the 3 concentrations significantly decreased the cell division rate at the exposure time of 48 h when compared to their respective controls. The mitotic index of the exposure time of 48 h was statistically different when compared to the cell division rate of the exposure time of 24 h for all concentrations. The number of cell abnormalities observed was not statistically significant by means of the chi-square test used. Therefore, under the conditions analyzed, the 3 concentrations tested for the cork cambium of jatobá-do-cerrado significantly decreased, in statistical terms, the cell division rate in the meristematic cells of A. cepa roots. Este estudo avalia o extrato hidroalcoólico obtido do ritidoma de Hymenaea stigonocarpa (jatobá-do-cerrado) sobre as células meristemáticas de raízes de Allium cepa, em três concentrações, 0,5; 1,0 e 1,5 mg/mL, e dois tempos de exposição, 24 h e 48 h. As lâminas foram preparadas pela técnica de esmagamento. As células foram analisadas em todo ciclo celular, totalizando 5.000 para cada grupo controle e concentração. Verificou-se que as três concentrações diminuíram significativamente o índice de divisão celular no tempo de exposição de 48 h quando comparadas aos seus respectivos controles. O índice mitótico do tempo de exposição de 48 h foi estatisticamente diferente quando comparado ao índice de divisão celular do tempo de exposição de 24 h em todas as concentrações. O número de anormalidades celulares observado não foi estatisticamente significativo pelo teste do qui-quadrado utilizado. Portanto, nas condições analisadas, as três concentrações testadas do ritidoma do jatobá-do-cerrado reduziram de forma estatisticamente significativa o índice de divisão celular das células meristemáticas de raízes de A. cepa.Universidade Federal de Santa Catarina2014-11-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p4510.5007/2175-7925.2015v28n1p45Biotemas; v. 28 n. 1 (2015); 45-492175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCenghttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p45/28660Copyright (c) 2015 Layana Menezes da Silva, Ana Paula Peron, Francisco Ronielson da Silva Carvalho, Livia Martins, Iana Felício Bantim Calou, Hélio Barros Fernandesinfo:eu-repo/semantics/openAccessSilva, Layana Menezes daPeron, Ana PaulaCarvalho, Francisco Ronielson da SilvaMartins, LiviaCalou, Iana Felício BantimFernandes, Hélio Barros2017-10-19T16:43:26Zoai:periodicos.ufsc.br:article/33844Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2017-10-19T16:43:26Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots Efeito antiproliferativo do extrato hidroalcoólico de Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) sobre as células meristemáticas de raízes de Allium cepa L |
title |
Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots |
spellingShingle |
Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots Silva, Layana Menezes da |
title_short |
Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots |
title_full |
Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots |
title_fullStr |
Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots |
title_full_unstemmed |
Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots |
title_sort |
Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots |
author |
Silva, Layana Menezes da |
author_facet |
Silva, Layana Menezes da Peron, Ana Paula Carvalho, Francisco Ronielson da Silva Martins, Livia Calou, Iana Felício Bantim Fernandes, Hélio Barros |
author_role |
author |
author2 |
Peron, Ana Paula Carvalho, Francisco Ronielson da Silva Martins, Livia Calou, Iana Felício Bantim Fernandes, Hélio Barros |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Layana Menezes da Peron, Ana Paula Carvalho, Francisco Ronielson da Silva Martins, Livia Calou, Iana Felício Bantim Fernandes, Hélio Barros |
description |
This study evaluates the hydroalcoholic extract obtained from the cork cambium of Hymenaea stigonocarpa (jatobá-do-cerrado) on the meristematic cells of Allium cepa roots, at 3 concentrations, 0.5, 1.0, and 1.5 mg/mL, and 2 exposure times, 24 h and 48 h. The slides were made through the crushing technique. Cells were analyzed throughout the cell cycle, totaling 5,000 for each control and concentration group. We found that the 3 concentrations significantly decreased the cell division rate at the exposure time of 48 h when compared to their respective controls. The mitotic index of the exposure time of 48 h was statistically different when compared to the cell division rate of the exposure time of 24 h for all concentrations. The number of cell abnormalities observed was not statistically significant by means of the chi-square test used. Therefore, under the conditions analyzed, the 3 concentrations tested for the cork cambium of jatobá-do-cerrado significantly decreased, in statistical terms, the cell division rate in the meristematic cells of A. cepa roots. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-11-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p45 10.5007/2175-7925.2015v28n1p45 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p45 |
identifier_str_mv |
10.5007/2175-7925.2015v28n1p45 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p45/28660 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 28 n. 1 (2015); 45-49 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940553476931584 |