Molecular characterization of Prosopis juliflora SW. DC. through molecular markers and genetic similarity indices

• Autor(a) principal: Meira, Messulan Rodrigues
• Data de Publicação: 2020
• Outros Autores: Santos, Luiz Henrique Tolentino, Anjos, Quirlian Queite Araújo, Santos, Brenda Emilly Ferreira, Guimarães, Leonardo de Sousa, Cerqueira Silva, Carlos Bernard Moreno
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Biotemas (Online)
• Texto Completo: https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2020.e70282

Prosopis juliflora (Sx.) DC., commonly known as algarroba, is an adapted tree species in Brazil with animal food value and environmental value in reforestation. However, little is known about its molecular genetic diversity. Therefore, the aim of our study was to use different similarity measures based on the genetic characterization of algarroba obtained through ISSR and RGA markers in naturalized individuals in southwestern Bahia. Young leaves of 36 algarroba individuals were collected in the municipality of Itapetinga, Bahia. DNA was extracted by the 10% SDS protocol and quantified by the absorbance ratio A260/A230 and A260/A280 nm. Ten pairs of RGA and 23 primers of ISSR were used for PCR, and the amplified products were electrophoresed on 2% agarose gels for 2 h. The genotyping results (binary matrix) were used to calculate the Jaccard, Simple Matching and Sorensen Dice similarity coefficients. Finally the UPGMA clustering, Tocher optimization and PCA were performed. We observed for ISSR and RGA 205 and 68 marks, respectively, with expected heterozygosity of 0.28 and 0.21; polymorphic information content was 91.71 and 0.17% and genetic distance 8.0 and 8.53, respectively. These findings contribute to our understanding of the wide distribution and adaptation of this species in reforested areas.