Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño

Detalhes bibliográficos
Autor(a) principal: Chudzikiewicz, Andrei
Data de Publicação: 2019
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UFSC
Texto Completo: https://repositorio.ufsc.br/handle/123456789/197790
Resumo: TCC (graduação)- Universidade Federal de Santa Catarina. Campus Curitibanos. Agronomia.
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spelling Universidade Federal de Santa CatarinaChudzikiewicz, AndreiDal Vesco, Lírio Luiz2019-07-16T19:28:50Z2019-07-16T19:28:50Z2019-06-28https://repositorio.ufsc.br/handle/123456789/197790TCC (graduação)- Universidade Federal de Santa Catarina. Campus Curitibanos. Agronomia.O bambu é uma gramínea perene de rápido crescimento e alta resiliência. Usada na construção civil, fabricação de móveis, alimentação humana e animal, dentre outras aplicações com grande potencial agrícola. É uma planta propagada por estacas ou desdobramento de touceiras e é restrito o número de brotos gerados pelo método de multiplicação convencional, sendo assim, faz-se necessário o uso de alternativas, como por exemplo, as técnicas de cultura de tecidos para produção de grande número de mudas. Para tanto, é necessário estabelecer protocolos para introdução, estabelecimento e proliferação de explantes. Este trabalho teve como objetivo testar diferentes métodos de desinfestação de gemas na introdução in vitro Bambusa. oldhamii e avaliar o efeito do uso de fitorreguladores na multiplicação in vitro de Guadua chacoensis. Gemas de colmos jovens de B. oldhamii foram submetidos a diferentes tratamentos de desinfestação utilizando um esquema fatorial de 2x3 com seis tratamentos, onde se testou diferentes doses e tempos de exposição em álcool 70%; Hipoclorito de sódio-NaClO (0,5 e 1,5%); fungicidas: Metiltiofan (0,6 g L-1) + Captan (0,6 g L-1). Em seguida, após a excisão do tecido matriz e exposição das gemas, os explantes foram inoculados em meio de cultura MS sem ou com carvão ativado (1,5 g L-1). Posteriormente as gemas que não apresentaram contaminação foram subcultivadas em meio de cultura MS-Murashige e Skoog com presença de 5,0 µM de 6-benzilaminopurina-BAP com ou sem plant preservative mixture-PPM (2 ml L-1). Para a multiplicação do G. chacoensis foi utilizado um esquema fatorial de 2x3 com seis tratamentos com duas doses de ácido 1-naftaleno acético-ANA (0,0 e 0,1 µM) combinadas com três doses de Thidiazuron-TDZ (0,0; 0,1; 1,0 µM) suplementados ao meio de cultura MS. Os resultados de todos os tratamentos não foram estatisticamente diferentes. O uso e carvão ativado no estabelecimento in vitro de B. oldhamii não promoveu redução na oxidação. Gemas de B. oldhamii desinfestadas e estabelecidas em meio de cultura MS com ou sem carvão ativado, quando subcultivadas em novos meios e suplementados com 5,0 µM de BAP, com ou sem a adição de PPM resultam na indução e alongamento dos brotos. A suplementação de TDZ ao meio de cultura MS independente da concentração e combinação com ANA, para a multiplicação in vitro de G. chacoensis não interferiram na formação de novas estruturas morfológicas para o período de 29 dias de cultivo.Bamboo is a perennial grass of fast growth and high resilience. Used in construction, furniture manufacturing, human and animal feeding, among other applications with great agricultural potential. It is a plant propagated by cuttings or unfolding of clumps and the number of shoots generated by the conventional multiplication method is restricted, so it is necessary to use alternatives such as tissue culture techniques to produce large number of plantlets. However, it is necessary to establish protocols for the introduction, establishment and proliferation of explants. This work aimed to test different methods of bud disinfestation in the in vitro introduction Bambusa oldhamii and to evaluate the effect of the use of plant regulators in the in vitro multiplication of Guadua chacoensis. Buds extracted from young sprouts of B. oldhamii were submitted to different disinfestation treatments using a 2x3 factorial scheme with six treatments, where different doses and times of exposure in 70% alcohol were tested; NaClO (0.5 and 1.5%); fungicides: Methylthiophan (0.6 g L-1) + Captan (0.6 g L-1). Then, after excision of the matrix tissue and exposure of the buds, the explants were inoculated in MS culture medium without or with activated charcoal (1.5 g L-1). Subsequently, the non-contaminated bud was subcultured in MS-Murashige and Skoog culture media containing 5.0 μM 6-benzylaminopurine-BAP with or without plant preservative mixture-PPM (2 ml L-1). For the multiplication of G. chacoensis, a 2x3 factorial scheme was used with six treatments with two doses of 1-naphthalene acetic acid-NAA (0.0 and 0.1 μM) combined with three doses of Thidiazuron-TDZ (0.0, 0.1, 1.0 μM) supplemented with the MS culture medium. The results of all treatments were not statistically different. The use and activated charcoal in the in vitro establishment of B. oldhamii did not promote reduction in the oxidation. B. oldhamii bud disinfested and established in MS medium with or without activated carbon, when subcultured in new media and supplemented with 5.0 μM of BAP, with or without the addition of PPM result in induction and elongation of shoots. TDZ supplementation to the MS culture medium independent of concentration and combination with ANA for the in vitro multiplication of G. chacoensis did not interfere in the formation of new morphological structures for the period of 29 days of cultivation.Curitibanos, SCmicropropagaçãobiotecnologiabambusachacoensisoldhamiiguaduaEstabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoñoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFSCinstname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCLICENSElicense.txtlicense.txttext/plain; charset=utf-81383https://repositorio.ufsc.br/bitstream/123456789/197790/6/license.txt11ee89cd31d893362820eab7c4d46734MD56ORIGINALAndrei Chudzikiewicz.pdfAndrei Chudzikiewicz.pdfTCC Andrei Chudzikiewicz, micropropagação de bambuapplication/pdf2071032https://repositorio.ufsc.br/bitstream/123456789/197790/5/Andrei%20Chudzikiewicz.pdfb49f8e26cf1bedcd66d56628bddb7548MD55123456789/1977902019-07-16 16:28:52.111oai:repositorio.ufsc.br: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ório de PublicaçõesPUBhttp://150.162.242.35/oai/requestopendoar:23732019-07-16T19:28:52Repositório Institucional da UFSC - Universidade Federal de Santa Catarina (UFSC)false
dc.title.pt_BR.fl_str_mv Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño
title Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño
spellingShingle Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño
Chudzikiewicz, Andrei
micropropagação
biotecnologia
bambusa
chacoensis
oldhamii
guadua
title_short Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño
title_full Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño
title_fullStr Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño
title_full_unstemmed Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño
title_sort Estabelecimento in vitro de Bambusa oldhamii Munro e Multiplicação in vitro de Guadua chacoensis (Rojas) Londoño
author Chudzikiewicz, Andrei
author_facet Chudzikiewicz, Andrei
author_role author
dc.contributor.pt_BR.fl_str_mv Universidade Federal de Santa Catarina
dc.contributor.author.fl_str_mv Chudzikiewicz, Andrei
dc.contributor.advisor1.fl_str_mv Dal Vesco, Lírio Luiz
contributor_str_mv Dal Vesco, Lírio Luiz
dc.subject.por.fl_str_mv micropropagação
biotecnologia
bambusa
chacoensis
oldhamii
guadua
topic micropropagação
biotecnologia
bambusa
chacoensis
oldhamii
guadua
description TCC (graduação)- Universidade Federal de Santa Catarina. Campus Curitibanos. Agronomia.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-07-16T19:28:50Z
dc.date.available.fl_str_mv 2019-07-16T19:28:50Z
dc.date.issued.fl_str_mv 2019-06-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufsc.br/handle/123456789/197790
url https://repositorio.ufsc.br/handle/123456789/197790
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv Bamboo is a perennial grass of fast growth and high resilience. Used in construction, furniture manufacturing, human and animal feeding, among other applications with great agricultural potential. It is a plant propagated by cuttings or unfolding of clumps and the number of shoots generated by the conventional multiplication method is restricted, so it is necessary to use alternatives such as tissue culture techniques to produce large number of plantlets. However, it is necessary to establish protocols for the introduction, establishment and proliferation of explants. This work aimed to test different methods of bud disinfestation in the in vitro introduction Bambusa oldhamii and to evaluate the effect of the use of plant regulators in the in vitro multiplication of Guadua chacoensis. Buds extracted from young sprouts of B. oldhamii were submitted to different disinfestation treatments using a 2x3 factorial scheme with six treatments, where different doses and times of exposure in 70% alcohol were tested; NaClO (0.5 and 1.5%); fungicides: Methylthiophan (0.6 g L-1) + Captan (0.6 g L-1). Then, after excision of the matrix tissue and exposure of the buds, the explants were inoculated in MS culture medium without or with activated charcoal (1.5 g L-1). Subsequently, the non-contaminated bud was subcultured in MS-Murashige and Skoog culture media containing 5.0 μM 6-benzylaminopurine-BAP with or without plant preservative mixture-PPM (2 ml L-1). For the multiplication of G. chacoensis, a 2x3 factorial scheme was used with six treatments with two doses of 1-naphthalene acetic acid-NAA (0.0 and 0.1 μM) combined with three doses of Thidiazuron-TDZ (0.0, 0.1, 1.0 μM) supplemented with the MS culture medium. The results of all treatments were not statistically different. The use and activated charcoal in the in vitro establishment of B. oldhamii did not promote reduction in the oxidation. B. oldhamii bud disinfested and established in MS medium with or without activated carbon, when subcultured in new media and supplemented with 5.0 μM of BAP, with or without the addition of PPM result in induction and elongation of shoots. TDZ supplementation to the MS culture medium independent of concentration and combination with ANA for the in vitro multiplication of G. chacoensis did not interfere in the formation of new morphological structures for the period of 29 days of cultivation.
dc.publisher.none.fl_str_mv Curitibanos, SC
publisher.none.fl_str_mv Curitibanos, SC
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFSC
instname:Universidade Federal de Santa Catarina (UFSC)
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instname_str Universidade Federal de Santa Catarina (UFSC)
instacron_str UFSC
institution UFSC
reponame_str Repositório Institucional da UFSC
collection Repositório Institucional da UFSC
bitstream.url.fl_str_mv https://repositorio.ufsc.br/bitstream/123456789/197790/6/license.txt
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