Efeitos da agmatina em modelos animais de depressão e mania

Detalhes bibliográficos
Autor(a) principal: Budni, Josiane
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFSC
Texto Completo: http://repositorio.ufsc.br/xmlui/handle/123456789/91961
Resumo: Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas. Programa de Pós-Graduação em Neurociências.
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spelling Efeitos da agmatina em modelos animais de depressão e maniaNeurociênciasAgmatinaPotassioOuabaínaEstresse OxidativoDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas. Programa de Pós-Graduação em Neurociências.Mood disorders are chronic and severe mental disorders. Many compounds, as agmatine, have been investigated regarding their effects and underlying mechanisms in major depression and bipolar disorder. The administration of agmatine, an endogenous cationic amine, elicits an antidepressant-like effect in the mouse forced swimming test (FST) by a mechanism dependent on the inhibition of the NMDA receptors and the L-arginine-nitric oxide (NO) pathway. Since it has been reported that the NO can activate different types of potassium (K+) channels in several tissues, the present study investigated the possibility of synergistic interactions between different types of K+ channel inhibitors and agmatine in the FST. Treatment of mice by i.c.v. route with subeffective doses of tetraethylammonium (a non specific inhibitor of K+ channels, 25 pg/site), glibenclamide (an ATP-sensitive K+ channel inhibitor, 0.5 pg/site), charybdotoxin (a large- and intermediate-conductance calciumactivated K+ channel inhibitor, 25 pg/site) or apamin (a small-conductance calcium-activated K+ channel inhibitor, 10 pg/site), augmented the effect of agmatine (0.001 mg/kg, i.p.) in the FST. Furthermore, the administration of agmatine and the K+ channel inhibitors, alone or in combination, did not affect locomotion in the open-field test. Moreover, the reduction in the immobility time elicited by an active dose of agmatine (10 mg/kg, i.p.) in the FST was prevented by the pretreatment of mice with the K+ channel openers cromakalim (10 ìg/site, i.c.v.) and minoxidil (10 ìg/site, i.c.v.), without affecting locomotion. These results raise the possibility that the antidepressant-like effect of agmatine in the FST is related to its modulatory effects on neuronal excitability, via inhibition of K+ channels. Furthermore, we investigated the effect of agmatine in the ouabain-induced hyperactivity in rats, an animal model of mania. In this study, the pretreatment of the animals with agmatine (0.1, 1 and 10 mg/kg, p.o. administered twice a day for 7 days) was able to attenuate the behavioral and neurochemical changes elicited by acute administration of ouabain, a Na,K-ATPase-inhibiting compound, given by i.c.v. route, in Wistar rats. Ouabain (10 ìM/site) significantly increased motor activity in the open-field test. The pretreatment with lithium chloride (LiCl, 45 mg/kg, p.o.) for seven days completely prevented the hyperactivity, but agmatine (0,1-10 mg/kg) partially prevented the ouabain-induced hyperlocomotion. Ouabain treatment elicited lipid peroxidation (increased TBARS levels) and reduced the glutathione peroxidase (GPx) activity in the hippocampus and glutathione reductase (GR) activity in the cerebral cortex and hippocampus, effects that were completely prevented in rats pretreated with agmatine and LiCl. These results show that agmatine, in a way similar to LiCl, is able to prevent the neurochemical alterations observed in the ouabain-induced model of mania in rats, but was able to reverse only partially the uabain-induced hyperlocomotion. Together, these results suggest that agmatine has not only antidepressant-like effects through an interaction with K+ channels, but also antimanic propierties. Investigar o envolvimeto dos canais de K+ no mecanismo de ação da agmatina no teste do nado forçado e o efeito da agmatina em um modelo animal de mania induzido por ouabaína e sua relação com o estresse oxidativo.Florianópolis, SCRodrigues, Ana Lucia SeveroUniversidade Federal de Santa CatarinaBudni, Josiane2012-10-24T04:20:09Z2012-10-24T04:20:09Z20082008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis1 v.| il., grafs.application/pdf249817http://repositorio.ufsc.br/xmlui/handle/123456789/91961porreponame:Repositório Institucional da UFSCinstname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCinfo:eu-repo/semantics/openAccess2013-05-02T04:27:46Zoai:repositorio.ufsc.br:123456789/91961Repositório InstitucionalPUBhttp://150.162.242.35/oai/requestopendoar:23732013-05-02T04:27:46Repositório Institucional da UFSC - Universidade Federal de Santa Catarina (UFSC)false
dc.title.none.fl_str_mv Efeitos da agmatina em modelos animais de depressão e mania
title Efeitos da agmatina em modelos animais de depressão e mania
spellingShingle Efeitos da agmatina em modelos animais de depressão e mania
Budni, Josiane
Neurociências
Agmatina
Potassio
Ouabaína
Estresse Oxidativo
title_short Efeitos da agmatina em modelos animais de depressão e mania
title_full Efeitos da agmatina em modelos animais de depressão e mania
title_fullStr Efeitos da agmatina em modelos animais de depressão e mania
title_full_unstemmed Efeitos da agmatina em modelos animais de depressão e mania
title_sort Efeitos da agmatina em modelos animais de depressão e mania
author Budni, Josiane
author_facet Budni, Josiane
author_role author
dc.contributor.none.fl_str_mv Rodrigues, Ana Lucia Severo
Universidade Federal de Santa Catarina
dc.contributor.author.fl_str_mv Budni, Josiane
dc.subject.por.fl_str_mv Neurociências
Agmatina
Potassio
Ouabaína
Estresse Oxidativo
topic Neurociências
Agmatina
Potassio
Ouabaína
Estresse Oxidativo
description Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas. Programa de Pós-Graduação em Neurociências.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008
2012-10-24T04:20:09Z
2012-10-24T04:20:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv 249817
http://repositorio.ufsc.br/xmlui/handle/123456789/91961
identifier_str_mv 249817
url http://repositorio.ufsc.br/xmlui/handle/123456789/91961
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1 v.| il., grafs.
application/pdf
dc.publisher.none.fl_str_mv Florianópolis, SC
publisher.none.fl_str_mv Florianópolis, SC
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFSC
instname:Universidade Federal de Santa Catarina (UFSC)
instacron:UFSC
instname_str Universidade Federal de Santa Catarina (UFSC)
instacron_str UFSC
institution UFSC
reponame_str Repositório Institucional da UFSC
collection Repositório Institucional da UFSC
repository.name.fl_str_mv Repositório Institucional da UFSC - Universidade Federal de Santa Catarina (UFSC)
repository.mail.fl_str_mv
_version_ 1808652129450065920

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