Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFSC |
Texto Completo: | https://repositorio.ufsc.br/handle/123456789/200457 |
Resumo: | While many studies have shown that pulp mill effluents can affect ovarian physiology in fish, far fewer studies have considered the effects in males. We conducted a lab study to examine the effects of effluent from a Brazilian pulp and paper mill on hepatic and testicular morphology and various aspects of testicular physiology in the zebrafish Danio rerio. Males were exposed to lab water (control) or 4% effluent for 14 days. Effluent exposure did not affect testis size as measured by the gonadosomatic index, but contributed to morphological changes in the seminiferous tubules. The number of cysts with histopathological changes was elevated in effluent-exposed fish and the number of cysts containing spermatids was significantly reduced. The testis of effluent exposed fish had reduced levels of lactate, elevated lactate dehydrogenase activity, increased levels of reactive oxygen species and reduced levels of phosphorylated P38 mitogen-activated protein kinase (pP38 MAPK). Separate studies showed that the addition of lactate to testicular tissue incubated in vitro increased the activation of P38 MAPK. Effluent exposure also increased vacuolization, necrosis, apoptosis, hyperemia, and fat infiltration of the hepatocytes. Collectively, we provide evidence of short term effects of pulp mill effluent on testicular and hepatic physiology and biochemistry in the zebrafish. |
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Universidade Federal de Santa Catarina. Centro de Filosofia e Ciências Humanas. Laboratório de Análise AmbientalCastro, A. J. G;Baptista, I. E.;De Moura, K. R. S.Padilha, F.;Tonietto, J.;De Souza, A. Z. P.;Soares, C. H. L.;Silva, F. R. M. B.;Van Der Kraak, G.2019-09-09T16:10:46Z2019-09-09T16:10:46Z2018-04-01doi.org/10.1016/j.cbpc.2018.02.005https://repositorio.ufsc.br/handle/123456789/200457While many studies have shown that pulp mill effluents can affect ovarian physiology in fish, far fewer studies have considered the effects in males. We conducted a lab study to examine the effects of effluent from a Brazilian pulp and paper mill on hepatic and testicular morphology and various aspects of testicular physiology in the zebrafish Danio rerio. Males were exposed to lab water (control) or 4% effluent for 14 days. Effluent exposure did not affect testis size as measured by the gonadosomatic index, but contributed to morphological changes in the seminiferous tubules. The number of cysts with histopathological changes was elevated in effluent-exposed fish and the number of cysts containing spermatids was significantly reduced. The testis of effluent exposed fish had reduced levels of lactate, elevated lactate dehydrogenase activity, increased levels of reactive oxygen species and reduced levels of phosphorylated P38 mitogen-activated protein kinase (pP38 MAPK). Separate studies showed that the addition of lactate to testicular tissue incubated in vitro increased the activation of P38 MAPK. Effluent exposure also increased vacuolization, necrosis, apoptosis, hyperemia, and fat infiltration of the hepatocytes. Collectively, we provide evidence of short term effects of pulp mill effluent on testicular and hepatic physiology and biochemistry in the zebrafish.TestisBiochemistryLactateLactate dehydrogenaseP38 mitogenactivated protein kinaseOxidative stressMorphologyExposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafishinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSCinstname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-8270https://repositorio.ufsc.br/bitstream/123456789/200457/2/license.txt9acdea492e736458829e2c4c3844e4d9MD52ORIGINALExposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish.pdfExposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish.pdfArtigoapplication/pdf1043482https://repositorio.ufsc.br/bitstream/123456789/200457/1/Exposure%20to%20a%20Brazilian%20pulp%20mill%20effluent%20impacts%20the%20testis%20and%20liver%20in%20the%20zebrafish.pdf9db621179baaca75d495ed717fd15864MD51123456789/2004572019-09-09 13:10:46.934oai:repositorio.ufsc.br:123456789/200457QXRyaWJ1acOnw6NvLU7Do29Db21lcmNpYWwtQ29tcGFydGlsaGFJZ3VhbCAgQ0MgQlktTkMtU0EgRXN0YSBsaWNlbsOnYSBwZXJtaXRlIHF1ZSBvdXRyb3MgcmVtaXhlbSwgYWRhcHRlbSBlIGNyaWVtIGEgcGFydGlyIGRvIHNldSB0cmFiYWxobyBwYXJhIGZpbnMgbsOjbyBjb21lcmNpYWlzLCBkZXNkZSBxdWUgYXRyaWJ1YW0gYSB2b2PDqiBvIGRldmlkbyBjcsOpZGl0byBlIHF1ZSBsaWNlbmNpZW0gYXMgbm92YXMgY3JpYcOnw7VlcyBzb2IgdGVybW9zIGlkw6pudGljb3MuRepositório de PublicaçõesPUBhttp://150.162.242.35/oai/requestopendoar:23732019-09-09T16:10:46Repositório Institucional da UFSC - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.pt_BR.fl_str_mv |
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish |
title |
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish |
spellingShingle |
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish Castro, A. J. G; Testis Biochemistry Lactate Lactate dehydrogenase P38 mitogenactivated protein kinase Oxidative stress Morphology |
title_short |
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish |
title_full |
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish |
title_fullStr |
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish |
title_full_unstemmed |
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish |
title_sort |
Exposure to a Brazilian pulp mill effluent impacts the testis and liver in the zebrafish |
author |
Castro, A. J. G; |
author_facet |
Castro, A. J. G; Baptista, I. E.; De Moura, K. R. S. Padilha, F.; Tonietto, J.; De Souza, A. Z. P.; Soares, C. H. L.; Silva, F. R. M. B.; Van Der Kraak, G. |
author_role |
author |
author2 |
Baptista, I. E.; De Moura, K. R. S. Padilha, F.; Tonietto, J.; De Souza, A. Z. P.; Soares, C. H. L.; Silva, F. R. M. B.; Van Der Kraak, G. |
author2_role |
author author author author author author author author |
dc.contributor.pt_BR.fl_str_mv |
Universidade Federal de Santa Catarina. Centro de Filosofia e Ciências Humanas. Laboratório de Análise Ambiental |
dc.contributor.author.fl_str_mv |
Castro, A. J. G; Baptista, I. E.; De Moura, K. R. S. Padilha, F.; Tonietto, J.; De Souza, A. Z. P.; Soares, C. H. L.; Silva, F. R. M. B.; Van Der Kraak, G. |
dc.subject.por.fl_str_mv |
Testis Biochemistry Lactate Lactate dehydrogenase P38 mitogenactivated protein kinase Oxidative stress Morphology |
topic |
Testis Biochemistry Lactate Lactate dehydrogenase P38 mitogenactivated protein kinase Oxidative stress Morphology |
description |
While many studies have shown that pulp mill effluents can affect ovarian physiology in fish, far fewer studies have considered the effects in males. We conducted a lab study to examine the effects of effluent from a Brazilian pulp and paper mill on hepatic and testicular morphology and various aspects of testicular physiology in the zebrafish Danio rerio. Males were exposed to lab water (control) or 4% effluent for 14 days. Effluent exposure did not affect testis size as measured by the gonadosomatic index, but contributed to morphological changes in the seminiferous tubules. The number of cysts with histopathological changes was elevated in effluent-exposed fish and the number of cysts containing spermatids was significantly reduced. The testis of effluent exposed fish had reduced levels of lactate, elevated lactate dehydrogenase activity, increased levels of reactive oxygen species and reduced levels of phosphorylated P38 mitogen-activated protein kinase (pP38 MAPK). Separate studies showed that the addition of lactate to testicular tissue incubated in vitro increased the activation of P38 MAPK. Effluent exposure also increased vacuolization, necrosis, apoptosis, hyperemia, and fat infiltration of the hepatocytes. Collectively, we provide evidence of short term effects of pulp mill effluent on testicular and hepatic physiology and biochemistry in the zebrafish. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-04-01 |
dc.date.accessioned.fl_str_mv |
2019-09-09T16:10:46Z |
dc.date.available.fl_str_mv |
2019-09-09T16:10:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufsc.br/handle/123456789/200457 |
dc.identifier.issn.none.fl_str_mv |
doi.org/10.1016/j.cbpc.2018.02.005 |
identifier_str_mv |
doi.org/10.1016/j.cbpc.2018.02.005 |
url |
https://repositorio.ufsc.br/handle/123456789/200457 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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