Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria

Detalhes bibliográficos
Autor(a) principal: Horner, Rosmari
Data de Publicação: 2009
Outros Autores: Kocourek, Graziela E. Dobbert, Domingues, Vanessa Oliveira, Rigatti, Fabiane, Bertoncheli, Claudia de Mello, Paraguinski, Gustavo Luiz
Tipo de documento: Artigo
Idioma: por
Título da fonte: Saúde (Santa Maria)
Texto Completo: https://periodicos.ufsm.br/revistasaude/article/view/6491
Resumo: The urinary tract infections are the most common infectious diseases in clinical practice and, they are also the most frequent at the hospital setting. The objective of this article was to compare the methods: 2,3,5-trifeniltetrazólio (CTT), GRAM staining, and physical-chemical tests, as a screening for urine culture. Sixty-nine samples of urine from the Maringá district, Santa Maria-RS collected from February to July 2007were analyzed. All of them had the medical request for urine culture. For the purpose of comparison, 104 samples collected in the hospital from May to July were sent to the microbiology sector of the HUSM. Ten (14.5%) out of the 69 community samples showed positive results in the culture, while 17 (16.36%) from the 104 hospitals samples were positive. The CTT was positive in 5 (50%) of 10 positive samples of the community, and 8 (47.1%) of the hospital samples. From the results obtained we concluded that the method of GRAM staining is more effective, when compared with the evidence of nitrite and leukocytes; once it presents better agreement with the urine culture and the lowest level of interference.
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spelling Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa MariaComparação de métodos de triagem para detecção de bacteriúria em amostras do bairro Maringá e do Hospital Universitário de Santa MariaBacteriuriaInfections235-trifeniltetrazólio.BacteriúriaInfecção235-trifeniltetrazólio.The urinary tract infections are the most common infectious diseases in clinical practice and, they are also the most frequent at the hospital setting. The objective of this article was to compare the methods: 2,3,5-trifeniltetrazólio (CTT), GRAM staining, and physical-chemical tests, as a screening for urine culture. Sixty-nine samples of urine from the Maringá district, Santa Maria-RS collected from February to July 2007were analyzed. All of them had the medical request for urine culture. For the purpose of comparison, 104 samples collected in the hospital from May to July were sent to the microbiology sector of the HUSM. Ten (14.5%) out of the 69 community samples showed positive results in the culture, while 17 (16.36%) from the 104 hospitals samples were positive. The CTT was positive in 5 (50%) of 10 positive samples of the community, and 8 (47.1%) of the hospital samples. From the results obtained we concluded that the method of GRAM staining is more effective, when compared with the evidence of nitrite and leukocytes; once it presents better agreement with the urine culture and the lowest level of interference.As infecções do trato urinário estão entre as doenças infecciosas mais comuns na prática clínica, sendo que no meio hospitalar são as mais freqüentes. Esta pesquisa teve por objetivo comparar as seguintes metodologias de triagem utilizadas nas uroculturas: o cloreto de 2,3,5-trifeniltetrazólio (CTT), a coloração de Gram e testes físico-químicos. De fevereiro a julho de 2007 foram analisadas 69 amostras de urina provenientes do Bairro Maringá, Santa Maria-RS, com solicitação médica do exame de urocultura. Para fins de comparação com amostras hospitalares, de maio a julho de 2007 foram efetuadas 104 culturas de urina encaminhadas ao setor de Microbiologia do HUSM. Das 69 amostras comunitárias 10 (14,5%) tiveram resultado cultural positivo, e das 104 amostras hospitalares, 17 (16,36%). O CTT foi positivo em 5 (50%) das 10 amostras positivas comunitárias, e 8 (47,1%) das  17 hospitalares. Concluímos que o método de triagem pela coloração de Gram é mais eficaz comparado com as provas de nitrito e leucócitos, por apresentar melhor concordância com a urocultura e menor índice de interferentes.Universidade Federal de Santa Maria2009-01-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/revistasaude/article/view/649110.5902/223658346491Saúde (Santa Maria); Revista do Centro de Ciências da Saúde. Vol. 34 - n. 1 e 2, 2008; 16-21Saúde (Santa Maria); Revista do Centro de Ciências da Saúde. Vol. 34 - n. 1 e 2, 2008; 16-212236-58340103-4499reponame:Saúde (Santa Maria)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/revistasaude/article/view/6491/3944Horner, RosmariKocourek, Graziela E. DobbertDomingues, Vanessa OliveiraRigatti, FabianeBertoncheli, Claudia de MelloParaguinski, Gustavo Luizinfo:eu-repo/semantics/openAccess2020-03-16T16:56:30Zoai:ojs.pkp.sfu.ca:article/6491Revistahttps://periodicos.ufsm.br/revistasaudePUBhttps://periodicos.ufsm.br/revistasaude/oairevistasaude.ufsm@gmail.com || amanda.revsaude@gmail.com || beatriz.revsaude@gmail.com2236-58342236-5834opendoar:2020-03-16T16:56:30Saúde (Santa Maria) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria
Comparação de métodos de triagem para detecção de bacteriúria em amostras do bairro Maringá e do Hospital Universitário de Santa Maria
title Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria
spellingShingle Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria
Horner, Rosmari
Bacteriuria
Infections
2
3
5-trifeniltetrazólio.
Bacteriúria
Infecção
2
3
5-trifeniltetrazólio.
title_short Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria
title_full Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria
title_fullStr Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria
title_full_unstemmed Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria
title_sort Comparison of methods of trial for detection of bacteriuria in samples from the Maringá district and the University Hospital of Santa Maria
author Horner, Rosmari
author_facet Horner, Rosmari
Kocourek, Graziela E. Dobbert
Domingues, Vanessa Oliveira
Rigatti, Fabiane
Bertoncheli, Claudia de Mello
Paraguinski, Gustavo Luiz
author_role author
author2 Kocourek, Graziela E. Dobbert
Domingues, Vanessa Oliveira
Rigatti, Fabiane
Bertoncheli, Claudia de Mello
Paraguinski, Gustavo Luiz
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Horner, Rosmari
Kocourek, Graziela E. Dobbert
Domingues, Vanessa Oliveira
Rigatti, Fabiane
Bertoncheli, Claudia de Mello
Paraguinski, Gustavo Luiz
dc.subject.por.fl_str_mv Bacteriuria
Infections
2
3
5-trifeniltetrazólio.
Bacteriúria
Infecção
2
3
5-trifeniltetrazólio.
topic Bacteriuria
Infections
2
3
5-trifeniltetrazólio.
Bacteriúria
Infecção
2
3
5-trifeniltetrazólio.
description The urinary tract infections are the most common infectious diseases in clinical practice and, they are also the most frequent at the hospital setting. The objective of this article was to compare the methods: 2,3,5-trifeniltetrazólio (CTT), GRAM staining, and physical-chemical tests, as a screening for urine culture. Sixty-nine samples of urine from the Maringá district, Santa Maria-RS collected from February to July 2007were analyzed. All of them had the medical request for urine culture. For the purpose of comparison, 104 samples collected in the hospital from May to July were sent to the microbiology sector of the HUSM. Ten (14.5%) out of the 69 community samples showed positive results in the culture, while 17 (16.36%) from the 104 hospitals samples were positive. The CTT was positive in 5 (50%) of 10 positive samples of the community, and 8 (47.1%) of the hospital samples. From the results obtained we concluded that the method of GRAM staining is more effective, when compared with the evidence of nitrite and leukocytes; once it presents better agreement with the urine culture and the lowest level of interference.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/revistasaude/article/view/6491
10.5902/223658346491
url https://periodicos.ufsm.br/revistasaude/article/view/6491
identifier_str_mv 10.5902/223658346491
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/revistasaude/article/view/6491/3944
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Saúde (Santa Maria); Revista do Centro de Ciências da Saúde. Vol. 34 - n. 1 e 2, 2008; 16-21
Saúde (Santa Maria); Revista do Centro de Ciências da Saúde. Vol. 34 - n. 1 e 2, 2008; 16-21
2236-5834
0103-4499
reponame:Saúde (Santa Maria)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Saúde (Santa Maria)
collection Saúde (Santa Maria)
repository.name.fl_str_mv Saúde (Santa Maria) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv revistasaude.ufsm@gmail.com || amanda.revsaude@gmail.com || beatriz.revsaude@gmail.com
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