Cultive tissue of Salvia hispanica L.

Detalhes bibliográficos
Autor(a) principal: Arfux, Claudia Regina Baur
Data de Publicação: 2015
Outros Autores: Menegati, Celia de Fátima, Silva, Renata Moreira da, Rondon, Josimara Nolasco, Gabriel, Valdinez, Costa, Francilina Araujo, Carvalho, Cristiano Marcelo Espinola, Souza, Pricila Fatima de, Silva, Cristiane Bezerra da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET)
Texto Completo: https://periodicos.ufsm.br/reget/article/view/15566
Resumo: Chia (Salvia hispanica L.) is an annual herbaceous plant native to Mexico in pre-Columbian times was used a lot with food and medicinal purpose, but during the process of domestication suffered habitat loss due to degradation of natural resources. It has high contente of omega-6 and omega-3, potent antioxidants, protein, dietary fiber, α-linoleic, widely used in weight reduction treatments, and reduction of cholesterol and its price is always high (100 g seed costs on average £ 25.00). The objective was to evaluate the production of seedlings of S. hispanica from embryos in culture media with and without sucrose. Held at 120 seed disinfection detergent Tween 80 for 2 minutes soaking in 1% sodium hypochlorite for 5 minutes in 70% ethanol for 2 min followed by rinsing three times with sterile distilled water. After this procedure, the seeds were placed in glass vials with 5 mL of liquid MS medium plus 30 g L-1 sucrose 100 mg L-inositol 1 pH adjusted to 5.8 without and without phytoregulator agar. Was used as control MS medium without sucrose and without agar. The cultures were kept in a growth chamber at 25 ± 2 ° C under a photoperiod of 16 hours. Each vial contained 30 seeds with 4 replications. We evaluated the germination and early growth of the seedling root and hypocotyl. 60 seedlings per treatment were used for evaluation of measures with a millimeter ruler on the length of the shoot and root. Germination was always higher in cultures with sucrose. Plantlets from 14 days had higher growth of shoots and roots in MS medium with sucrose. Sucrose directly affects seedling development of chia and may impair normal growth and promote somaclonal variation.
id UFSM-15_2389701417a27000561d90f74fd00d80
oai_identifier_str oai:ojs.pkp.sfu.ca:article/15566
network_acronym_str UFSM-15
network_name_str Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET)
repository_id_str
spelling Cultive tissue of Salvia hispanica L.Cultivo in vitro de Salvia hispanica L.Culture mediumSacaroseDevelopment in vitroMeio de cultivoSacaroseDesenvolvimento in vitroChia (Salvia hispanica L.) is an annual herbaceous plant native to Mexico in pre-Columbian times was used a lot with food and medicinal purpose, but during the process of domestication suffered habitat loss due to degradation of natural resources. It has high contente of omega-6 and omega-3, potent antioxidants, protein, dietary fiber, α-linoleic, widely used in weight reduction treatments, and reduction of cholesterol and its price is always high (100 g seed costs on average £ 25.00). The objective was to evaluate the production of seedlings of S. hispanica from embryos in culture media with and without sucrose. Held at 120 seed disinfection detergent Tween 80 for 2 minutes soaking in 1% sodium hypochlorite for 5 minutes in 70% ethanol for 2 min followed by rinsing three times with sterile distilled water. After this procedure, the seeds were placed in glass vials with 5 mL of liquid MS medium plus 30 g L-1 sucrose 100 mg L-inositol 1 pH adjusted to 5.8 without and without phytoregulator agar. Was used as control MS medium without sucrose and without agar. The cultures were kept in a growth chamber at 25 ± 2 ° C under a photoperiod of 16 hours. Each vial contained 30 seeds with 4 replications. We evaluated the germination and early growth of the seedling root and hypocotyl. 60 seedlings per treatment were used for evaluation of measures with a millimeter ruler on the length of the shoot and root. Germination was always higher in cultures with sucrose. Plantlets from 14 days had higher growth of shoots and roots in MS medium with sucrose. Sucrose directly affects seedling development of chia and may impair normal growth and promote somaclonal variation.A chia (Salvia hispanica L.) é uma planta herbácea anual originária do México que em tempos pré colombianos foi muito utilizada com finalidade alimentar e medicinal, mas durante o processo de domesticação sofreu perda de habitat devido a degradação de recursos naturais. Tem alto teor de ômega-6 e ômega-3, potentes antioxidantes, proteínas, fibra alimentar, α-linolêico, muito utilizada nos tratamentos de redução de peso, e redução de colesterol  e seu preço está sempre em alta (100 g de sementes custa em média R$ 25,00).O objetivo foi avaliar a produção de plântulas de S. hispanica a partir de embriões em meios de cultivo com e sem sacarose. Realizou-se assepsia em 120 sementes com detergente Tween 80 por 2 minutos, imersão em solução de hipoclorito de sódio 1% por 5 minutos, em álcool 70% por 2 minutos, seguido de triplo enxágüe com água destilada  esterilizada. Após esse procedimento, as sementes foram colocadas em frascos de vidro com 5 mL de meio MS liquido, acrescido de 30 g L-1 de sacarose, 100 mg L-1 de inositol com o pH ajustado para 5,8, sem fitorregulador e sem ágar. Como controle usou-se meio de cultivo MS sem sacarose e sem ágar. Os cultivos foram mantidos em sala de crescimento, a 25 ± 2 ºC, sob fotoperíodo de 16 horas. Cada frasco continha 30 sementes com 4 repetições. Avaliou-se a germinação e o crescimento inicial de hipocótilo e raiz das plântulas. Foram utilizadas 60 plântulas por tratamento para avaliação de medidas com régua milimetrada quanto ao comprimento caulinar e de raiz. A germinação sempre foi maior em cultivo com sacarose. Plântulas cultivadas partir de 14 dias tiveram maior crescimento da parte aérea e radicular em meio MS com sacarose. A sacarose afeta diretamente o desenvolvimento de plântulas de chia e pode comprometer o crescimento normal e promover variação somaclonal.Universidade Federal de Santa Maria2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/reget/article/view/1556610.5902/2236117015566Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental; REGET, V. 19, N. 2, MAY-AUG., 2015; 1555-1560Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental; REGET, V. 19, N. 2, MAIO-AGO., 2015; 1555-15602236-11702236-1170reponame:Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/reget/article/view/15566/pdfhttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessArfux, Claudia Regina BaurMenegati, Celia de FátimaSilva, Renata Moreira daRondon, Josimara NolascoGabriel, ValdinezCosta, Francilina AraujoCarvalho, Cristiano Marcelo EspinolaSouza, Pricila Fatima deSilva, Cristiane Bezerra da2022-09-14T16:51:31Zoai:ojs.pkp.sfu.ca:article/15566Revistahttps://periodicos.ufsm.br/regetPUBhttps://periodicos.ufsm.br/reget/oaimarcelobdarosa@gmail.com||reget.ufsm@gmail.com2236-11702236-1170opendoar:2022-09-14T16:51:31Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Cultive tissue of Salvia hispanica L.
Cultivo in vitro de Salvia hispanica L.
title Cultive tissue of Salvia hispanica L.
spellingShingle Cultive tissue of Salvia hispanica L.
Arfux, Claudia Regina Baur
Culture medium
Sacarose
Development in vitro
Meio de cultivo
Sacarose
Desenvolvimento in vitro
title_short Cultive tissue of Salvia hispanica L.
title_full Cultive tissue of Salvia hispanica L.
title_fullStr Cultive tissue of Salvia hispanica L.
title_full_unstemmed Cultive tissue of Salvia hispanica L.
title_sort Cultive tissue of Salvia hispanica L.
author Arfux, Claudia Regina Baur
author_facet Arfux, Claudia Regina Baur
Menegati, Celia de Fátima
Silva, Renata Moreira da
Rondon, Josimara Nolasco
Gabriel, Valdinez
Costa, Francilina Araujo
Carvalho, Cristiano Marcelo Espinola
Souza, Pricila Fatima de
Silva, Cristiane Bezerra da
author_role author
author2 Menegati, Celia de Fátima
Silva, Renata Moreira da
Rondon, Josimara Nolasco
Gabriel, Valdinez
Costa, Francilina Araujo
Carvalho, Cristiano Marcelo Espinola
Souza, Pricila Fatima de
Silva, Cristiane Bezerra da
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Arfux, Claudia Regina Baur
Menegati, Celia de Fátima
Silva, Renata Moreira da
Rondon, Josimara Nolasco
Gabriel, Valdinez
Costa, Francilina Araujo
Carvalho, Cristiano Marcelo Espinola
Souza, Pricila Fatima de
Silva, Cristiane Bezerra da
dc.subject.por.fl_str_mv Culture medium
Sacarose
Development in vitro
Meio de cultivo
Sacarose
Desenvolvimento in vitro
topic Culture medium
Sacarose
Development in vitro
Meio de cultivo
Sacarose
Desenvolvimento in vitro
description Chia (Salvia hispanica L.) is an annual herbaceous plant native to Mexico in pre-Columbian times was used a lot with food and medicinal purpose, but during the process of domestication suffered habitat loss due to degradation of natural resources. It has high contente of omega-6 and omega-3, potent antioxidants, protein, dietary fiber, α-linoleic, widely used in weight reduction treatments, and reduction of cholesterol and its price is always high (100 g seed costs on average £ 25.00). The objective was to evaluate the production of seedlings of S. hispanica from embryos in culture media with and without sucrose. Held at 120 seed disinfection detergent Tween 80 for 2 minutes soaking in 1% sodium hypochlorite for 5 minutes in 70% ethanol for 2 min followed by rinsing three times with sterile distilled water. After this procedure, the seeds were placed in glass vials with 5 mL of liquid MS medium plus 30 g L-1 sucrose 100 mg L-inositol 1 pH adjusted to 5.8 without and without phytoregulator agar. Was used as control MS medium without sucrose and without agar. The cultures were kept in a growth chamber at 25 ± 2 ° C under a photoperiod of 16 hours. Each vial contained 30 seeds with 4 replications. We evaluated the germination and early growth of the seedling root and hypocotyl. 60 seedlings per treatment were used for evaluation of measures with a millimeter ruler on the length of the shoot and root. Germination was always higher in cultures with sucrose. Plantlets from 14 days had higher growth of shoots and roots in MS medium with sucrose. Sucrose directly affects seedling development of chia and may impair normal growth and promote somaclonal variation.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/reget/article/view/15566
10.5902/2236117015566
url https://periodicos.ufsm.br/reget/article/view/15566
identifier_str_mv 10.5902/2236117015566
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/reget/article/view/15566/pdf
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental; REGET, V. 19, N. 2, MAY-AUG., 2015; 1555-1560
Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental; REGET, V. 19, N. 2, MAIO-AGO., 2015; 1555-1560
2236-1170
2236-1170
reponame:Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET)
collection Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET)
repository.name.fl_str_mv Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv marcelobdarosa@gmail.com||reget.ufsm@gmail.com
_version_ 1799950575581790208