Cultive tissue of Salvia hispanica L.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET) |
Texto Completo: | https://periodicos.ufsm.br/reget/article/view/15566 |
Resumo: | Chia (Salvia hispanica L.) is an annual herbaceous plant native to Mexico in pre-Columbian times was used a lot with food and medicinal purpose, but during the process of domestication suffered habitat loss due to degradation of natural resources. It has high contente of omega-6 and omega-3, potent antioxidants, protein, dietary fiber, α-linoleic, widely used in weight reduction treatments, and reduction of cholesterol and its price is always high (100 g seed costs on average £ 25.00). The objective was to evaluate the production of seedlings of S. hispanica from embryos in culture media with and without sucrose. Held at 120 seed disinfection detergent Tween 80 for 2 minutes soaking in 1% sodium hypochlorite for 5 minutes in 70% ethanol for 2 min followed by rinsing three times with sterile distilled water. After this procedure, the seeds were placed in glass vials with 5 mL of liquid MS medium plus 30 g L-1 sucrose 100 mg L-inositol 1 pH adjusted to 5.8 without and without phytoregulator agar. Was used as control MS medium without sucrose and without agar. The cultures were kept in a growth chamber at 25 ± 2 ° C under a photoperiod of 16 hours. Each vial contained 30 seeds with 4 replications. We evaluated the germination and early growth of the seedling root and hypocotyl. 60 seedlings per treatment were used for evaluation of measures with a millimeter ruler on the length of the shoot and root. Germination was always higher in cultures with sucrose. Plantlets from 14 days had higher growth of shoots and roots in MS medium with sucrose. Sucrose directly affects seedling development of chia and may impair normal growth and promote somaclonal variation. |
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Cultive tissue of Salvia hispanica L.Cultivo in vitro de Salvia hispanica L.Culture mediumSacaroseDevelopment in vitroMeio de cultivoSacaroseDesenvolvimento in vitroChia (Salvia hispanica L.) is an annual herbaceous plant native to Mexico in pre-Columbian times was used a lot with food and medicinal purpose, but during the process of domestication suffered habitat loss due to degradation of natural resources. It has high contente of omega-6 and omega-3, potent antioxidants, protein, dietary fiber, α-linoleic, widely used in weight reduction treatments, and reduction of cholesterol and its price is always high (100 g seed costs on average £ 25.00). The objective was to evaluate the production of seedlings of S. hispanica from embryos in culture media with and without sucrose. Held at 120 seed disinfection detergent Tween 80 for 2 minutes soaking in 1% sodium hypochlorite for 5 minutes in 70% ethanol for 2 min followed by rinsing three times with sterile distilled water. After this procedure, the seeds were placed in glass vials with 5 mL of liquid MS medium plus 30 g L-1 sucrose 100 mg L-inositol 1 pH adjusted to 5.8 without and without phytoregulator agar. Was used as control MS medium without sucrose and without agar. The cultures were kept in a growth chamber at 25 ± 2 ° C under a photoperiod of 16 hours. Each vial contained 30 seeds with 4 replications. We evaluated the germination and early growth of the seedling root and hypocotyl. 60 seedlings per treatment were used for evaluation of measures with a millimeter ruler on the length of the shoot and root. Germination was always higher in cultures with sucrose. Plantlets from 14 days had higher growth of shoots and roots in MS medium with sucrose. Sucrose directly affects seedling development of chia and may impair normal growth and promote somaclonal variation.A chia (Salvia hispanica L.) é uma planta herbácea anual originária do México que em tempos pré colombianos foi muito utilizada com finalidade alimentar e medicinal, mas durante o processo de domesticação sofreu perda de habitat devido a degradação de recursos naturais. Tem alto teor de ômega-6 e ômega-3, potentes antioxidantes, proteínas, fibra alimentar, α-linolêico, muito utilizada nos tratamentos de redução de peso, e redução de colesterol e seu preço está sempre em alta (100 g de sementes custa em média R$ 25,00).O objetivo foi avaliar a produção de plântulas de S. hispanica a partir de embriões em meios de cultivo com e sem sacarose. Realizou-se assepsia em 120 sementes com detergente Tween 80 por 2 minutos, imersão em solução de hipoclorito de sódio 1% por 5 minutos, em álcool 70% por 2 minutos, seguido de triplo enxágüe com água destilada esterilizada. Após esse procedimento, as sementes foram colocadas em frascos de vidro com 5 mL de meio MS liquido, acrescido de 30 g L-1 de sacarose, 100 mg L-1 de inositol com o pH ajustado para 5,8, sem fitorregulador e sem ágar. Como controle usou-se meio de cultivo MS sem sacarose e sem ágar. Os cultivos foram mantidos em sala de crescimento, a 25 ± 2 ºC, sob fotoperíodo de 16 horas. Cada frasco continha 30 sementes com 4 repetições. Avaliou-se a germinação e o crescimento inicial de hipocótilo e raiz das plântulas. Foram utilizadas 60 plântulas por tratamento para avaliação de medidas com régua milimetrada quanto ao comprimento caulinar e de raiz. A germinação sempre foi maior em cultivo com sacarose. Plântulas cultivadas partir de 14 dias tiveram maior crescimento da parte aérea e radicular em meio MS com sacarose. A sacarose afeta diretamente o desenvolvimento de plântulas de chia e pode comprometer o crescimento normal e promover variação somaclonal.Universidade Federal de Santa Maria2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/reget/article/view/1556610.5902/2236117015566Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental; REGET, V. 19, N. 2, MAY-AUG., 2015; 1555-1560Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental; REGET, V. 19, N. 2, MAIO-AGO., 2015; 1555-15602236-11702236-1170reponame:Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/reget/article/view/15566/pdfhttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessArfux, Claudia Regina BaurMenegati, Celia de FátimaSilva, Renata Moreira daRondon, Josimara NolascoGabriel, ValdinezCosta, Francilina AraujoCarvalho, Cristiano Marcelo EspinolaSouza, Pricila Fatima deSilva, Cristiane Bezerra da2022-09-14T16:51:31Zoai:ojs.pkp.sfu.ca:article/15566Revistahttps://periodicos.ufsm.br/regetPUBhttps://periodicos.ufsm.br/reget/oaimarcelobdarosa@gmail.com||reget.ufsm@gmail.com2236-11702236-1170opendoar:2022-09-14T16:51:31Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Cultive tissue of Salvia hispanica L. Cultivo in vitro de Salvia hispanica L. |
title |
Cultive tissue of Salvia hispanica L. |
spellingShingle |
Cultive tissue of Salvia hispanica L. Arfux, Claudia Regina Baur Culture medium Sacarose Development in vitro Meio de cultivo Sacarose Desenvolvimento in vitro |
title_short |
Cultive tissue of Salvia hispanica L. |
title_full |
Cultive tissue of Salvia hispanica L. |
title_fullStr |
Cultive tissue of Salvia hispanica L. |
title_full_unstemmed |
Cultive tissue of Salvia hispanica L. |
title_sort |
Cultive tissue of Salvia hispanica L. |
author |
Arfux, Claudia Regina Baur |
author_facet |
Arfux, Claudia Regina Baur Menegati, Celia de Fátima Silva, Renata Moreira da Rondon, Josimara Nolasco Gabriel, Valdinez Costa, Francilina Araujo Carvalho, Cristiano Marcelo Espinola Souza, Pricila Fatima de Silva, Cristiane Bezerra da |
author_role |
author |
author2 |
Menegati, Celia de Fátima Silva, Renata Moreira da Rondon, Josimara Nolasco Gabriel, Valdinez Costa, Francilina Araujo Carvalho, Cristiano Marcelo Espinola Souza, Pricila Fatima de Silva, Cristiane Bezerra da |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Arfux, Claudia Regina Baur Menegati, Celia de Fátima Silva, Renata Moreira da Rondon, Josimara Nolasco Gabriel, Valdinez Costa, Francilina Araujo Carvalho, Cristiano Marcelo Espinola Souza, Pricila Fatima de Silva, Cristiane Bezerra da |
dc.subject.por.fl_str_mv |
Culture medium Sacarose Development in vitro Meio de cultivo Sacarose Desenvolvimento in vitro |
topic |
Culture medium Sacarose Development in vitro Meio de cultivo Sacarose Desenvolvimento in vitro |
description |
Chia (Salvia hispanica L.) is an annual herbaceous plant native to Mexico in pre-Columbian times was used a lot with food and medicinal purpose, but during the process of domestication suffered habitat loss due to degradation of natural resources. It has high contente of omega-6 and omega-3, potent antioxidants, protein, dietary fiber, α-linoleic, widely used in weight reduction treatments, and reduction of cholesterol and its price is always high (100 g seed costs on average £ 25.00). The objective was to evaluate the production of seedlings of S. hispanica from embryos in culture media with and without sucrose. Held at 120 seed disinfection detergent Tween 80 for 2 minutes soaking in 1% sodium hypochlorite for 5 minutes in 70% ethanol for 2 min followed by rinsing three times with sterile distilled water. After this procedure, the seeds were placed in glass vials with 5 mL of liquid MS medium plus 30 g L-1 sucrose 100 mg L-inositol 1 pH adjusted to 5.8 without and without phytoregulator agar. Was used as control MS medium without sucrose and without agar. The cultures were kept in a growth chamber at 25 ± 2 ° C under a photoperiod of 16 hours. Each vial contained 30 seeds with 4 replications. We evaluated the germination and early growth of the seedling root and hypocotyl. 60 seedlings per treatment were used for evaluation of measures with a millimeter ruler on the length of the shoot and root. Germination was always higher in cultures with sucrose. Plantlets from 14 days had higher growth of shoots and roots in MS medium with sucrose. Sucrose directly affects seedling development of chia and may impair normal growth and promote somaclonal variation. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/reget/article/view/15566 10.5902/2236117015566 |
url |
https://periodicos.ufsm.br/reget/article/view/15566 |
identifier_str_mv |
10.5902/2236117015566 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/reget/article/view/15566/pdf |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental; REGET, V. 19, N. 2, MAY-AUG., 2015; 1555-1560 Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental; REGET, V. 19, N. 2, MAIO-AGO., 2015; 1555-1560 2236-1170 2236-1170 reponame:Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET) |
collection |
Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET) |
repository.name.fl_str_mv |
Revista Eletrônica em Gestão Educação e Tecnologia Ambiental (REGET) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
marcelobdarosa@gmail.com||reget.ufsm@gmail.com |
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1799950575581790208 |