Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/14004 |
Resumo: | The equine market is constantly growing, so the search for perfection in breeding and for better specimens intensifies and the freezing of semen helps in this process, being an important tool to preserve the genetics of animals considered superior within their breeds. In this study the sperm selection through glass wool, centrifugation and Sperm Filter® were evaluated on the motility, vigor, pH, concentration, morphology and membrane integrity of the frozen equine semen. The semen of five Brazilian ponies was diluted in an extender based on skim milk powder at a dilution of 1:1 and distributed in three groups: centrifugation (C), glass wool filtration + centrifugation (LC) and glass wool filtration + Sperm Filter® (LS). Four ejaculates of each pony were used. In the three groups evaluated after thawing there was no difference in the parameters of local motility, vigor and pH. The highest percentage of total motility (52.50% ± 7.16 and 48.50% ± 5.87) (P <0.01) and progressive motility (41.75% ± 7.30 and 38.25% ± 6.93) (P <0.01) was detected in groups C and LC, respectively. When evaluating sperm membrane integrity, superior results were detected in groups C and LC in relation to LS (43.25% ± 7.48, 42.35% ± 8.39 and 29.10% ± 12.31) (P <0.01). Sperm concentration was higher in group C (79.65 ± 27.15 x106sptz/mL) than LC and LS (43.97 ± 18.67 x106sptz/ml and 25.95 ± 21.84 x106sptz/mL) (P <0.01). In the computerized evaluation of sperm motility there was no difference between groups. When evaluating sperm morphology, we found fewer morphological defects after sperm selection in relation to immediate sperm morphology after collection. It suggested that, when preparing the semen for freezing, the use of centrifugation or glass wool + centrifugation is the protocol of choice. |
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2018-08-03T19:03:12Z2018-08-03T19:03:12Z2018-02-28http://repositorio.ufsm.br/handle/1/14004The equine market is constantly growing, so the search for perfection in breeding and for better specimens intensifies and the freezing of semen helps in this process, being an important tool to preserve the genetics of animals considered superior within their breeds. In this study the sperm selection through glass wool, centrifugation and Sperm Filter® were evaluated on the motility, vigor, pH, concentration, morphology and membrane integrity of the frozen equine semen. The semen of five Brazilian ponies was diluted in an extender based on skim milk powder at a dilution of 1:1 and distributed in three groups: centrifugation (C), glass wool filtration + centrifugation (LC) and glass wool filtration + Sperm Filter® (LS). Four ejaculates of each pony were used. In the three groups evaluated after thawing there was no difference in the parameters of local motility, vigor and pH. The highest percentage of total motility (52.50% ± 7.16 and 48.50% ± 5.87) (P <0.01) and progressive motility (41.75% ± 7.30 and 38.25% ± 6.93) (P <0.01) was detected in groups C and LC, respectively. When evaluating sperm membrane integrity, superior results were detected in groups C and LC in relation to LS (43.25% ± 7.48, 42.35% ± 8.39 and 29.10% ± 12.31) (P <0.01). Sperm concentration was higher in group C (79.65 ± 27.15 x106sptz/mL) than LC and LS (43.97 ± 18.67 x106sptz/ml and 25.95 ± 21.84 x106sptz/mL) (P <0.01). In the computerized evaluation of sperm motility there was no difference between groups. When evaluating sperm morphology, we found fewer morphological defects after sperm selection in relation to immediate sperm morphology after collection. It suggested that, when preparing the semen for freezing, the use of centrifugation or glass wool + centrifugation is the protocol of choice.O mercado de equinos está em constante crescimento, com isso a busca pelo aperfeiçoamento na criação e por melhores exemplares se intensifica e o congelamento de sêmen auxilia nesse processo, sendo uma importante ferramenta para preservar a genética de animais considerados superiores dentro de suas raças. Neste estudo avaliou-se a técnica de seleção espermática através da lã de vidro, centrifugação e Sperm Filter® sobre a motilidade, vigor, pH, concentração, morfologia e integridade de membrana do sêmen equino congelado. O sêmen de cinco pôneis da raça Brasileira foi diluído em diluente a base de leite em pó desnatado na diluição de 1:1 e distribuído em três grupos: centrifugação (C), filtração em lã de vidro + centrifugação (LC) e filtração em lã de vidro + Sperm Filter® (LS). Foram utilizados quatro ejaculados de cada pônei. Nos três grupos avaliados após o descongelamento não houve diferença nos parâmetros de motilidade local, vigor e pH. O percentual maior de motilidade total (52,50% ± 7,16 e 48,50% ± 5,87) (P<0,01) e motilidade progressiva (41,75% ± 7,30 e 38,25% ± 6,93) (P< 0,01) foi detectado nos grupos C e LC, respectivamente. Ao avaliar a integridade da membrana espermática, resultados superiores foram detectados nos grupos C e LC em relação ao LS (43,25% ± 7,48, 42,35% ± 8,39 e 29,10% ± 12,31) (P<0,01). A concentração espermática foi superior no grupo C (79,65 ± 27,15 x106sptz/mL) em relação ao LC e LS (43,97 ± 18,67 x 106sptz/ml e 25,95 ± 21,84 x106sptz/mL) (P<0,01). Na avaliação computadorizada da motilidade espermática, não houve diferença entre os grupos. Ao avaliar a morfologia espermática, encontrou-se menos defeitos morfológicos após as etapas de seleção espermática em relação a morfologia espermática imediata após a coleta. Sugere-se que, quando da preparação do sêmen para congelamento, o uso da centrifugação ou lã de vidro + centrifugação seja o protocolo de eleição.porUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCriopreservaçãoSeleção espermáticaGaranhãoCryopreservationSperm selectionstallionCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAAvaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidroEvaluation of parameters pre and post-freeze equine semen after spermatozoa separation with the use of glass woolinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAntoniazzi, Alfredo Quiteshttp://lattes.cnpq.br/7258679373419746Pozzobon, Ricardohttp://lattes.cnpq.br/4294670606057446Löf, Henrique Kurtzhttp://lattes.cnpq.br/5671763020019533http://lattes.cnpq.br/7994516888104567Silva, Rômulo Oliveira Fernandes da500500000007600a338fc55-084a-47a0-8cef-889209277d3df3780311-1a9c-4972-b7b0-2f0213a1ec7937653533-ef4b-4dce-aa33-e55cfe63b0ccdd1bd923-1fa8-44bd-9af5-41aa17195022reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGMV_2018_SILVA_ROMULO.pdfDIS_PPGMV_2018_SILVA_ROMULO.pdfDissertação de Mestradoapplication/pdf942821http://repositorio.ufsm.br/bitstream/1/14004/1/DIS_PPGMV_2018_SILVA_ROMULO.pdfb4fef873d9fc92ecf78e15a3ff983016MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro |
dc.title.alternative.eng.fl_str_mv |
Evaluation of parameters pre and post-freeze equine semen after spermatozoa separation with the use of glass wool |
title |
Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro |
spellingShingle |
Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro Silva, Rômulo Oliveira Fernandes da Criopreservação Seleção espermática Garanhão Cryopreservation Sperm selection stallion CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro |
title_full |
Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro |
title_fullStr |
Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro |
title_full_unstemmed |
Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro |
title_sort |
Avaliação dos parâmetros do sêmen equino pré e pós-congelamento após separação dos espermatozoides com a utilização de lã de vidro |
author |
Silva, Rômulo Oliveira Fernandes da |
author_facet |
Silva, Rômulo Oliveira Fernandes da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Antoniazzi, Alfredo Quites |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7258679373419746 |
dc.contributor.referee1.fl_str_mv |
Pozzobon, Ricardo |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4294670606057446 |
dc.contributor.referee2.fl_str_mv |
Löf, Henrique Kurtz |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5671763020019533 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7994516888104567 |
dc.contributor.author.fl_str_mv |
Silva, Rômulo Oliveira Fernandes da |
contributor_str_mv |
Antoniazzi, Alfredo Quites Pozzobon, Ricardo Löf, Henrique Kurtz |
dc.subject.por.fl_str_mv |
Criopreservação Seleção espermática Garanhão |
topic |
Criopreservação Seleção espermática Garanhão Cryopreservation Sperm selection stallion CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Cryopreservation Sperm selection stallion |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The equine market is constantly growing, so the search for perfection in breeding and for better specimens intensifies and the freezing of semen helps in this process, being an important tool to preserve the genetics of animals considered superior within their breeds. In this study the sperm selection through glass wool, centrifugation and Sperm Filter® were evaluated on the motility, vigor, pH, concentration, morphology and membrane integrity of the frozen equine semen. The semen of five Brazilian ponies was diluted in an extender based on skim milk powder at a dilution of 1:1 and distributed in three groups: centrifugation (C), glass wool filtration + centrifugation (LC) and glass wool filtration + Sperm Filter® (LS). Four ejaculates of each pony were used. In the three groups evaluated after thawing there was no difference in the parameters of local motility, vigor and pH. The highest percentage of total motility (52.50% ± 7.16 and 48.50% ± 5.87) (P <0.01) and progressive motility (41.75% ± 7.30 and 38.25% ± 6.93) (P <0.01) was detected in groups C and LC, respectively. When evaluating sperm membrane integrity, superior results were detected in groups C and LC in relation to LS (43.25% ± 7.48, 42.35% ± 8.39 and 29.10% ± 12.31) (P <0.01). Sperm concentration was higher in group C (79.65 ± 27.15 x106sptz/mL) than LC and LS (43.97 ± 18.67 x106sptz/ml and 25.95 ± 21.84 x106sptz/mL) (P <0.01). In the computerized evaluation of sperm motility there was no difference between groups. When evaluating sperm morphology, we found fewer morphological defects after sperm selection in relation to immediate sperm morphology after collection. It suggested that, when preparing the semen for freezing, the use of centrifugation or glass wool + centrifugation is the protocol of choice. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-08-03T19:03:12Z |
dc.date.available.fl_str_mv |
2018-08-03T19:03:12Z |
dc.date.issued.fl_str_mv |
2018-02-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/14004 |
url |
http://repositorio.ufsm.br/handle/1/14004 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500500000007 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional Manancial UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
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Universidade Federal de Santa Maria (UFSM) |
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UFSM |
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UFSM |
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