Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5935 |
Resumo: | Oomycete Pythium insisiosum is the etiologic agent of pythiosis, chronic and granulomatous disease, which affects humans and animals in tropical and subtropical areas of the world. Due to ergosterol absence in the plasmatic membrane of this microorganism, treatments based on antifungal agents have been ineffective. Immunotherapy has emerged showing promising results. In this context, the improvement of the immunotherapy was the main goal of this study, whose first purpose was to characterize the immunodominant antigens from this species (P. insidosum ATCC 58637 and P. insidiosum 210-LAPEMI) through sodium dodecyl sulfate polyacrylamide gel eletrophoresis (SDS-PAGE). Some proteins showing 15 kDa to 120 kDa were detected, and bands were transferred to nitrocellulose membranes in order to develop the western blot tests. The second purpose was to characterize the humoral response to pythiosis in equine, in bovine and in experimental pythiosis in rabbits, considering the no treated disease, the disease cured by immunotherapy, the disease treated with immunotherapy but not cured, and the disease cured spontaneously. To encompass all these cases we have included sera from: a) horses cured by immunotherapy; b) horses with pythiosis but not responsive to immunotherapy; c) horses with pythiosis but not treated; d) bovines with pythiosis and showing spontaneous cure; e) rabbits with experimental pythiosis but not treated; and f) rabbits with experimental pythiosis treated with immunotherapy. All specimens recognized three immunodominant proteins: 74, 33 and 32 kDa. The sera from horses cured by immunotherapy (a) and bovine sera (d) recognized another immunodominant protein, 55 kDa, which was weakly positive or negative in the other groups. Thus, the 74, 33 and 32 kDa immunodominant proteins suggest a relevant function in the humoral response to pythiosis because they were recognized by horses, rabbits and bovines. In addition, the 55 kDa antigen, which is being reported here for the first time, is likely to be involved in the cure mechanisms stimulated by immunotherapy. |
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2013-05-202013-05-202010-07-26CAVALHEIRO, Patrícia Bernardes. CHARACTERIZATION OF IMMUNODOMINANT ANTIGEN OF PYTHIUM INSIDIOSUM RECOGNIZED BY ANTIBODIES OF HORSES, RABBITS AND CATTLE. 2010. 86 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/5935Oomycete Pythium insisiosum is the etiologic agent of pythiosis, chronic and granulomatous disease, which affects humans and animals in tropical and subtropical areas of the world. Due to ergosterol absence in the plasmatic membrane of this microorganism, treatments based on antifungal agents have been ineffective. Immunotherapy has emerged showing promising results. In this context, the improvement of the immunotherapy was the main goal of this study, whose first purpose was to characterize the immunodominant antigens from this species (P. insidosum ATCC 58637 and P. insidiosum 210-LAPEMI) through sodium dodecyl sulfate polyacrylamide gel eletrophoresis (SDS-PAGE). Some proteins showing 15 kDa to 120 kDa were detected, and bands were transferred to nitrocellulose membranes in order to develop the western blot tests. The second purpose was to characterize the humoral response to pythiosis in equine, in bovine and in experimental pythiosis in rabbits, considering the no treated disease, the disease cured by immunotherapy, the disease treated with immunotherapy but not cured, and the disease cured spontaneously. To encompass all these cases we have included sera from: a) horses cured by immunotherapy; b) horses with pythiosis but not responsive to immunotherapy; c) horses with pythiosis but not treated; d) bovines with pythiosis and showing spontaneous cure; e) rabbits with experimental pythiosis but not treated; and f) rabbits with experimental pythiosis treated with immunotherapy. All specimens recognized three immunodominant proteins: 74, 33 and 32 kDa. The sera from horses cured by immunotherapy (a) and bovine sera (d) recognized another immunodominant protein, 55 kDa, which was weakly positive or negative in the other groups. Thus, the 74, 33 and 32 kDa immunodominant proteins suggest a relevant function in the humoral response to pythiosis because they were recognized by horses, rabbits and bovines. In addition, the 55 kDa antigen, which is being reported here for the first time, is likely to be involved in the cure mechanisms stimulated by immunotherapy.O oomiceto Pythium insidiosum é o agente etiológico da pitiose, doença granulomatosa crônica que afeta animais domésticos (eqüinos, bovinos, etc) e o homem, em regiões tropicais e subtropicais de todo mundo. A ausência de ergosterol na membrana plasmática deste microorganismo torna a antifungicoterapia muito limitada, donde a imunoterapia (a base de hifas rompidas) tem emergido com resultados promissores. A identificação de antígenos imunodominantes é fundamental para a consolidação dos imunoterápicos utilizados no tratamento da pitiose. Objetivando-se o refinamento destes imunoterápicos, o presente estudo teve como proposta inicial, caracterizar os antígenos imunodominantes desta espécie (P. insidosum ATCC 58637 e P. insidiosum 210-LAPEMI) através de eletroforese em gel de poliacrilamida (SDS-PAGE). Diversas proteínas de 120 a 15 kDa foram detectadas e, a seguir, eletrotransferidas para membranas de nitrocelulose para realização de western blot. O segundo objetivo foi caracterizar a resposta humoral à doença em eqüinos, bovinos e na infecção experimental em coelhos, considerando-se a doença curada pela imunoterapia, doença tratada pela imunoterapia e não curada, doença não tratada, e na doença evidenciando cura espontânea. Para tanto, utilizou-se o soro de: a) eqüinos doentes e curados pela imunoterapia; b) eqüinos doentes e sem resposta a imunoterapia; c) eqüinos doentes e não tratados; d) bovinos doentes e com cura espontânea; e) coelhos infectados e tratados pela imunoterapia; f) coelhos infectados e não tratados. Todos os soros reconheceram três proteínas imunodominantes: 74, 33 e 32 kDa. O soro dos eqüinos doentes curados pela imunoterapia (a) e o soro dos bovinos (d) reconheceram também outra proteína imunodominante de 55 kDa, a qual, foi fracamente reconhecida ou ausente nos demais grupos. Assim, comprova-se que as proteínas de 74, 33 e 32 kDa desempenham relevante papel na resposta imune à pitiose, pois foram imunogênicas nas três espécies avaliadas. Em adição, sugere-se que a proteína de 55 kDa possa estar envolvida no mecanismo de cura promovido pela imunoterapia; ressalta-se que esta proteína é aqui pela primeira vez relatada.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBRAnálises Clínicas e ToxicológicasPythium insidiosumImunoterapiaProteínas imunodominantesPythium insidiosumImmunotherapyImmunodominant proteinsCNPQ::CIENCIAS DA SAUDE::FARMACIACaracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinosCharacterization of immunodominant antigen of Pythium insidiosum recognized by antibodies of horses, rabbits and cattleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAlves, Sydney Hartzhttp://lattes.cnpq.br/0330782478769631Santurio, Janio Moraishttp://lattes.cnpq.br/6316012260769979Pereira, Daniela Isabel Brayerhttp://lattes.cnpq.br/3382450720179401http://lattes.cnpq.br/4911392125230611Cavalheiro, Patrícia Bernardes201000000000400500500300300bfe6016c-9c1e-42ca-ab1d-db5453ed090c4dd4e59f-61cf-47f3-95a2-fc2b3d1dcb1ab3be69bf-7acc-480d-86ef-841f8e956de5d09ffaf4-4202-45bc-9969-4464c5afe88ainfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALCAVALHEIRO, PATRICIA BERNARDES.pdfapplication/pdf955804http://repositorio.ufsm.br/bitstream/1/5935/1/CAVALHEIRO%2c%20PATRICIA%20BERNARDES.pdf2e0dc6ff4500667f2b45c6cdd2038896MD51TEXTCAVALHEIRO, PATRICIA BERNARDES.pdf.txtCAVALHEIRO, PATRICIA BERNARDES.pdf.txtExtracted texttext/plain152898http://repositorio.ufsm.br/bitstream/1/5935/2/CAVALHEIRO%2c%20PATRICIA%20BERNARDES.pdf.txt9fa792e5b1cbc088f6c5b2e1301b68aeMD52THUMBNAILCAVALHEIRO, PATRICIA BERNARDES.pdf.jpgCAVALHEIRO, PATRICIA BERNARDES.pdf.jpgIM Thumbnailimage/jpeg4993http://repositorio.ufsm.br/bitstream/1/5935/3/CAVALHEIRO%2c%20PATRICIA%20BERNARDES.pdf.jpg80a457d1f1a179752ed3eebedbe81138MD531/59352022-10-11 11:56:21.516oai:repositorio.ufsm.br:1/5935Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-10-11T14:56:21Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos |
dc.title.alternative.eng.fl_str_mv |
Characterization of immunodominant antigen of Pythium insidiosum recognized by antibodies of horses, rabbits and cattle |
title |
Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos |
spellingShingle |
Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos Cavalheiro, Patrícia Bernardes Pythium insidiosum Imunoterapia Proteínas imunodominantes Pythium insidiosum Immunotherapy Immunodominant proteins CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos |
title_full |
Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos |
title_fullStr |
Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos |
title_full_unstemmed |
Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos |
title_sort |
Caracterização de antígenos imunodominantes de Pythium insidiosum reconhecidos por anticorpos de eqüinos, coelhos e bovinos |
author |
Cavalheiro, Patrícia Bernardes |
author_facet |
Cavalheiro, Patrícia Bernardes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Alves, Sydney Hartz |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0330782478769631 |
dc.contributor.referee1.fl_str_mv |
Santurio, Janio Morais |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6316012260769979 |
dc.contributor.referee2.fl_str_mv |
Pereira, Daniela Isabel Brayer |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3382450720179401 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4911392125230611 |
dc.contributor.author.fl_str_mv |
Cavalheiro, Patrícia Bernardes |
contributor_str_mv |
Alves, Sydney Hartz Santurio, Janio Morais Pereira, Daniela Isabel Brayer |
dc.subject.por.fl_str_mv |
Pythium insidiosum Imunoterapia Proteínas imunodominantes |
topic |
Pythium insidiosum Imunoterapia Proteínas imunodominantes Pythium insidiosum Immunotherapy Immunodominant proteins CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Pythium insidiosum Immunotherapy Immunodominant proteins |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Oomycete Pythium insisiosum is the etiologic agent of pythiosis, chronic and granulomatous disease, which affects humans and animals in tropical and subtropical areas of the world. Due to ergosterol absence in the plasmatic membrane of this microorganism, treatments based on antifungal agents have been ineffective. Immunotherapy has emerged showing promising results. In this context, the improvement of the immunotherapy was the main goal of this study, whose first purpose was to characterize the immunodominant antigens from this species (P. insidosum ATCC 58637 and P. insidiosum 210-LAPEMI) through sodium dodecyl sulfate polyacrylamide gel eletrophoresis (SDS-PAGE). Some proteins showing 15 kDa to 120 kDa were detected, and bands were transferred to nitrocellulose membranes in order to develop the western blot tests. The second purpose was to characterize the humoral response to pythiosis in equine, in bovine and in experimental pythiosis in rabbits, considering the no treated disease, the disease cured by immunotherapy, the disease treated with immunotherapy but not cured, and the disease cured spontaneously. To encompass all these cases we have included sera from: a) horses cured by immunotherapy; b) horses with pythiosis but not responsive to immunotherapy; c) horses with pythiosis but not treated; d) bovines with pythiosis and showing spontaneous cure; e) rabbits with experimental pythiosis but not treated; and f) rabbits with experimental pythiosis treated with immunotherapy. All specimens recognized three immunodominant proteins: 74, 33 and 32 kDa. The sera from horses cured by immunotherapy (a) and bovine sera (d) recognized another immunodominant protein, 55 kDa, which was weakly positive or negative in the other groups. Thus, the 74, 33 and 32 kDa immunodominant proteins suggest a relevant function in the humoral response to pythiosis because they were recognized by horses, rabbits and bovines. In addition, the 55 kDa antigen, which is being reported here for the first time, is likely to be involved in the cure mechanisms stimulated by immunotherapy. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-07-26 |
dc.date.accessioned.fl_str_mv |
2013-05-20 |
dc.date.available.fl_str_mv |
2013-05-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
CAVALHEIRO, Patrícia Bernardes. CHARACTERIZATION OF IMMUNODOMINANT ANTIGEN OF PYTHIUM INSIDIOSUM RECOGNIZED BY ANTIBODIES OF HORSES, RABBITS AND CATTLE. 2010. 86 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5935 |
identifier_str_mv |
CAVALHEIRO, Patrícia Bernardes. CHARACTERIZATION OF IMMUNODOMINANT ANTIGEN OF PYTHIUM INSIDIOSUM RECOGNIZED BY ANTIBODIES OF HORSES, RABBITS AND CATTLE. 2010. 86 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
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