Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/19100 |
Resumo: | The present dissertation aims to contribute to correct knowledge of biological activities of Nectandra grandiflora Nees & Mart. ex Nees essential oil (EO) and its major compound, (+)-dehydrofukinone (DHF). So, the potential this extract was verified to act as biostimulant in native woody species explants cultivated in vitro. Additionally, the antifungal potential of both N. grandiflora OE and its major constituent against wood rot fungi was determined, as well as its action on ergosterol content in the plasma membrane of phytopathogens. The EO was obtained from N. grandiflora leaves by hydrodistillation with modified Clevenger apparatus. Then, extraction yield was determined, and chemical composition was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The major constituent of OE was isolated by 4 chromatographic columns, carried out in sequence. For tests with the explants, Eugenia involucrata De Candolle nodal segments were inoculated in ½ MS medium, supplemented with N. grandiflora EO, previously diluted in ethanol, at .0.00, 0.05, 0.075, 0.1, 0.125, 0.15, 0.175 e 0.2 μL mL-1. The results showed that, when exposed to EO at 0.075 μL mL-1, the explants emitted a greater number of leaves when compared to the controls. For determination of N. grandiflora OE and its major constituent antifungal potential, the extractives previously diluted in ethanol were added to PDA medium. The antifungal potential was verified against white-rot fungi Ganoderma applanatum (Pers.) Pat. and Trametes versicolor (L.: Fr) Pilát, as well as brown-rot fungi Lentinus lepideus (Fr.) Fr. and Gloeophyllum trabeum (Pers.: Fr) Murrill. The EO was evaluated at 0.0, 0.5, 1.0 and 2.0 μL mL-1, whereas DHF was evaluated at the corresponding concentration to that found in 0.5 μL mL-1 EO (0.0971 μL mL-1). Regarding the results of mycelium growth inhibition, N. grandiflora EO completely inhibited growth of white-rot fungi T. versicolor, at 1.0 μL mL-1 EO, presenting the same behavior as positive control. The other fungal species did not have their growth affected by the extractives at the tested concentrations. Additionally, the results for the EO treatments did not differ from those obtained with the isolated substance, evidencing that the DHF is one of the substances responsible for the antifungal activity detected. Regarding the influence of the extractives on ergosterol content in plasma membrane of phytopathogens, the results indicated that both extractives evaluated caused a reduction of this sterol in T. versicolor and G. applanatum, indicating that their action target was the plasma membrane of whiterot fungi. Thus, the potential of N. grandiflora EO to act as possible biostimulant in the development of in vitro nodal segments of E. involucrata is highlighted, as well as to inhibit the growth of white-rot fungus T. versicolor. |
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2019-12-04T20:56:22Z2019-12-04T20:56:22Z2017-12-20http://repositorio.ufsm.br/handle/1/19100The present dissertation aims to contribute to correct knowledge of biological activities of Nectandra grandiflora Nees & Mart. ex Nees essential oil (EO) and its major compound, (+)-dehydrofukinone (DHF). So, the potential this extract was verified to act as biostimulant in native woody species explants cultivated in vitro. Additionally, the antifungal potential of both N. grandiflora OE and its major constituent against wood rot fungi was determined, as well as its action on ergosterol content in the plasma membrane of phytopathogens. The EO was obtained from N. grandiflora leaves by hydrodistillation with modified Clevenger apparatus. Then, extraction yield was determined, and chemical composition was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The major constituent of OE was isolated by 4 chromatographic columns, carried out in sequence. For tests with the explants, Eugenia involucrata De Candolle nodal segments were inoculated in ½ MS medium, supplemented with N. grandiflora EO, previously diluted in ethanol, at .0.00, 0.05, 0.075, 0.1, 0.125, 0.15, 0.175 e 0.2 μL mL-1. The results showed that, when exposed to EO at 0.075 μL mL-1, the explants emitted a greater number of leaves when compared to the controls. For determination of N. grandiflora OE and its major constituent antifungal potential, the extractives previously diluted in ethanol were added to PDA medium. The antifungal potential was verified against white-rot fungi Ganoderma applanatum (Pers.) Pat. and Trametes versicolor (L.: Fr) Pilát, as well as brown-rot fungi Lentinus lepideus (Fr.) Fr. and Gloeophyllum trabeum (Pers.: Fr) Murrill. The EO was evaluated at 0.0, 0.5, 1.0 and 2.0 μL mL-1, whereas DHF was evaluated at the corresponding concentration to that found in 0.5 μL mL-1 EO (0.0971 μL mL-1). Regarding the results of mycelium growth inhibition, N. grandiflora EO completely inhibited growth of white-rot fungi T. versicolor, at 1.0 μL mL-1 EO, presenting the same behavior as positive control. The other fungal species did not have their growth affected by the extractives at the tested concentrations. Additionally, the results for the EO treatments did not differ from those obtained with the isolated substance, evidencing that the DHF is one of the substances responsible for the antifungal activity detected. Regarding the influence of the extractives on ergosterol content in plasma membrane of phytopathogens, the results indicated that both extractives evaluated caused a reduction of this sterol in T. versicolor and G. applanatum, indicating that their action target was the plasma membrane of whiterot fungi. Thus, the potential of N. grandiflora EO to act as possible biostimulant in the development of in vitro nodal segments of E. involucrata is highlighted, as well as to inhibit the growth of white-rot fungus T. versicolor.A presente dissertação visa contribuir com o conhecimento acerca das atividades biológicas do óleo essencial (OE) de Nectandra grandiflora Nees & Mart. ex Nees e seu constituinte majoritário, a (+)-deidrofuquinona (DHF). Para isto, verificou-se o potencial deste extrativo para atuar como bioestimulante em explantes de uma espécie lenhosa nativa cultivada in vitro. Adicionalmente, determinou-se o potencial antifúngico tanto do OE de N. grandiflora quanto do seu constituinte majoritário frente a fungos apodrecedores da madeira, além de verificar sua ação sobre o conteúdo de ergosterol na membrana plasmática dos fitopatógenos. O OE foi obtido a partir das folhas de N. grandiflora por meio de hidrodestilação, com o auxílio do aparelho tipo Clevenger modificado. Em seguida, determinou-se o rendimento de extração e realizou-se a análise da composição química por cromatografia gasosa acoplada a espectrometria de massas (CG-EM). O constituinte majoritário do OE foi isolado por meio de 4 colunas cromatográficas, realizadas em sequência. Para os testes com os explantes, segmentos nodais de Eugenia involucrata De Candolle foram inoculados em meio nutritivo ½ MS, suplementado com o OE de N. grandiflora, previamente diluído em etanol, nas concentrações de 0,00, 0,05, 0,075, 0,10, 0,125, 0,150, 0,175 e 0,200 μL mL-1. Os resultados evidenciaram que, na concentração de 0,075 μL mL-1, os explantes emitiram um maior número de folhas quando comparados aos controles. Para a determinação do potencial antifúngico do OE de N. grandiflora e de seu constituinte majoritário, os extrativos, previamente diluídos em etanol, foram adicionados ao meio nutritivo BDA. O potencial antifúngico foi verificado frente aos fungos causadores de podridão-branca Ganoderma applanatum (Pers.) Pat. e Trametes versicolor (L.: Fr) Pilát, bem como frente aos fungos causadores de podridão-parda Lentinus lepideus (Fr.) Fr. e Gloeophyllum trabeum (Pers.: Fr) Murrill. O OE foi avaliado nas concentrações de 0,0, 0,5, 1,0 e 2,0 μL mL-1, enquanto que a DHF foi avaliada na concentração correspondente à encontrada em 0,5 μL mL-1 de OE (0,0971 μL mL-1). Quanto aos resultados de porcentagem de inibição do crescimento micelial, destaca-se que o OE de N. grandiflora inibiu totalmente o crescimento do fungo de podridão-branca T. versicolor a 1,0 μL mL-1, apresentando o mesmo comportamento que o controle positivo. As demais espécies fúngicas não tiveram seu crescimento afetado pelos extrativos nas concentrações avaliadas. Adicionalmente, os resultados encontrados nos tratamentos com OE não diferiram daqueles realizados com a substância isolada, evidenciando que a DHF é uma das substâncias responsáveis pela atividade antifúngica detectada. No que tange à influência dos extrativos sobre o conteúdo de ergosterol na membrana plasmática dos fitopatógenos, os resultados indicaram que ambos os extrativos avaliados causaram uma redução deste esterol para os fungos T. versicolor e G. applanatum, indicando ter como alvo de ação a membrana plasmática dos fungos causadores de podridão-branca. Com base no exposto, destaca-se o potencial do OE de N. grandiflora para atuar como um possível bioestimulante no desenvolvimento de segmentos nodais cultivados in vitro de E. involucrata, bem como para inibir o crescimento do fungo apodrecedor da madeira T. versicolor.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Engenharia FlorestalUFSMBrasilRecursos Florestais e Engenharia FlorestalAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCanela-amarelaMicropropagaçãoEugenia involucrata De CandolleAtividade antifúngicaPreservante da madeiraErgosterolLauraceaeMicropropagationAntifungal activityWood preservativeCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALÓleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitroNectandra grandiflora Nees & Mart. ex Nees essential oil: antifungal and bio-stimulating effect in in vitro culturesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisHeinzmann, Berta Mariahttp://lattes.cnpq.br/0786124562427815Santini, Elio Joséhttp://lattes.cnpq.br/1939844237237675Poletto, Igorhttp://lattes.cnpq.br/5851156257793439http://lattes.cnpq.br/1962877501336147Bianchini, Nadia Helena500200000003600ef52d431-0698-40fb-ab56-1dbd415cc5d7040d9383-dcbc-4639-a080-7cda07c2fe60c205518d-4830-438e-a329-e05916e246655dfc8864-aaf7-44f5-b487-910c74a8a17areponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro |
dc.title.alternative.eng.fl_str_mv |
Nectandra grandiflora Nees & Mart. ex Nees essential oil: antifungal and bio-stimulating effect in in vitro cultures |
title |
Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro |
spellingShingle |
Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro Bianchini, Nadia Helena Canela-amarela Micropropagação Eugenia involucrata De Candolle Atividade antifúngica Preservante da madeira Ergosterol Lauraceae Micropropagation Antifungal activity Wood preservative CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
title_short |
Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro |
title_full |
Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro |
title_fullStr |
Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro |
title_full_unstemmed |
Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro |
title_sort |
Óleo essencial de Nectandra grandiflora Nees & Mart. ex Nees: efeito antifúngico e bioestimulante em cultivos in vitro |
author |
Bianchini, Nadia Helena |
author_facet |
Bianchini, Nadia Helena |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Heinzmann, Berta Maria |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0786124562427815 |
dc.contributor.referee1.fl_str_mv |
Santini, Elio José |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1939844237237675 |
dc.contributor.referee2.fl_str_mv |
Poletto, Igor |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5851156257793439 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1962877501336147 |
dc.contributor.author.fl_str_mv |
Bianchini, Nadia Helena |
contributor_str_mv |
Heinzmann, Berta Maria Santini, Elio José Poletto, Igor |
dc.subject.por.fl_str_mv |
Canela-amarela Micropropagação Eugenia involucrata De Candolle Atividade antifúngica Preservante da madeira Ergosterol |
topic |
Canela-amarela Micropropagação Eugenia involucrata De Candolle Atividade antifúngica Preservante da madeira Ergosterol Lauraceae Micropropagation Antifungal activity Wood preservative CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
dc.subject.eng.fl_str_mv |
Lauraceae Micropropagation Antifungal activity Wood preservative |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
description |
The present dissertation aims to contribute to correct knowledge of biological activities of Nectandra grandiflora Nees & Mart. ex Nees essential oil (EO) and its major compound, (+)-dehydrofukinone (DHF). So, the potential this extract was verified to act as biostimulant in native woody species explants cultivated in vitro. Additionally, the antifungal potential of both N. grandiflora OE and its major constituent against wood rot fungi was determined, as well as its action on ergosterol content in the plasma membrane of phytopathogens. The EO was obtained from N. grandiflora leaves by hydrodistillation with modified Clevenger apparatus. Then, extraction yield was determined, and chemical composition was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The major constituent of OE was isolated by 4 chromatographic columns, carried out in sequence. For tests with the explants, Eugenia involucrata De Candolle nodal segments were inoculated in ½ MS medium, supplemented with N. grandiflora EO, previously diluted in ethanol, at .0.00, 0.05, 0.075, 0.1, 0.125, 0.15, 0.175 e 0.2 μL mL-1. The results showed that, when exposed to EO at 0.075 μL mL-1, the explants emitted a greater number of leaves when compared to the controls. For determination of N. grandiflora OE and its major constituent antifungal potential, the extractives previously diluted in ethanol were added to PDA medium. The antifungal potential was verified against white-rot fungi Ganoderma applanatum (Pers.) Pat. and Trametes versicolor (L.: Fr) Pilát, as well as brown-rot fungi Lentinus lepideus (Fr.) Fr. and Gloeophyllum trabeum (Pers.: Fr) Murrill. The EO was evaluated at 0.0, 0.5, 1.0 and 2.0 μL mL-1, whereas DHF was evaluated at the corresponding concentration to that found in 0.5 μL mL-1 EO (0.0971 μL mL-1). Regarding the results of mycelium growth inhibition, N. grandiflora EO completely inhibited growth of white-rot fungi T. versicolor, at 1.0 μL mL-1 EO, presenting the same behavior as positive control. The other fungal species did not have their growth affected by the extractives at the tested concentrations. Additionally, the results for the EO treatments did not differ from those obtained with the isolated substance, evidencing that the DHF is one of the substances responsible for the antifungal activity detected. Regarding the influence of the extractives on ergosterol content in plasma membrane of phytopathogens, the results indicated that both extractives evaluated caused a reduction of this sterol in T. versicolor and G. applanatum, indicating that their action target was the plasma membrane of whiterot fungi. Thus, the potential of N. grandiflora EO to act as possible biostimulant in the development of in vitro nodal segments of E. involucrata is highlighted, as well as to inhibit the growth of white-rot fungus T. versicolor. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-12-20 |
dc.date.accessioned.fl_str_mv |
2019-12-04T20:56:22Z |
dc.date.available.fl_str_mv |
2019-12-04T20:56:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/19100 |
url |
http://repositorio.ufsm.br/handle/1/19100 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500200000003 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Engenharia Florestal |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Recursos Florestais e Engenharia Florestal |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
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Universidade Federal de Santa Maria (UFSM) |
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UFSM |
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UFSM |
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Manancial - Repositório Digital da UFSM |
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Manancial - Repositório Digital da UFSM |
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repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
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