Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/30281 |
Resumo: | Mercury (Hg) is a toxic element that can accumulate in the environment and in the food chain due to its biomagnification and bioaccumulation. A high concentration of Hg in the human body can lead to health complications, compromising mainly the nervous system and the functioning of some organs. One of the forms of contamination by Hg is from the ingestion of contaminated food. Several studies report the contamination of the Madeira River, located in Porto Velho - Rondônia, by Hg that is used in gold mining. It should be noted that there is a considerable population of riverine people who use the river for fishing. The consumption of contaminated fish can be a public health issue, justifying the development of analytical methods to monitor Hg concentration levels in fish. In this sense, a sample introduction system with chemical vapor generation coupled to inductively coupled plasma mass spectrometry was developed for Hg determination in fish muscle. The reaction conditions and physical variables of the proposed system were evaluated and eighteen species of fish with different eating habits were analyzed, such as piscivores, scavengers, carnivores, omnivores and herbivores. The results were analyzed in accordance with current legislation in Brazil (ANVISA), which specifies maximum Hg values of 1.0 g g-1 for predatory species and 0.5 g g-1 for non-predatory species. The accuracy of the proposed method was evaluated using certified reference material Dogfish Liver Certified Reference Material for Trace Metals (DOLT-4) where an agreement of 95% (t-test) with certified value was achieved. The precision of the method is better than 5% (relative standard deviation) for ten consecutive measurements of Hg. The method detection and quantification limits were 0.04 ug g-1 and 0.06 ug g-1, respectively, which meets the maximum concentration of Hg in fish established by ANVISA. |
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2023-09-28T13:12:04Z2023-09-28T13:12:04Z2023-06-26http://repositorio.ufsm.br/handle/1/30281Mercury (Hg) is a toxic element that can accumulate in the environment and in the food chain due to its biomagnification and bioaccumulation. A high concentration of Hg in the human body can lead to health complications, compromising mainly the nervous system and the functioning of some organs. One of the forms of contamination by Hg is from the ingestion of contaminated food. Several studies report the contamination of the Madeira River, located in Porto Velho - Rondônia, by Hg that is used in gold mining. It should be noted that there is a considerable population of riverine people who use the river for fishing. The consumption of contaminated fish can be a public health issue, justifying the development of analytical methods to monitor Hg concentration levels in fish. In this sense, a sample introduction system with chemical vapor generation coupled to inductively coupled plasma mass spectrometry was developed for Hg determination in fish muscle. The reaction conditions and physical variables of the proposed system were evaluated and eighteen species of fish with different eating habits were analyzed, such as piscivores, scavengers, carnivores, omnivores and herbivores. The results were analyzed in accordance with current legislation in Brazil (ANVISA), which specifies maximum Hg values of 1.0 g g-1 for predatory species and 0.5 g g-1 for non-predatory species. The accuracy of the proposed method was evaluated using certified reference material Dogfish Liver Certified Reference Material for Trace Metals (DOLT-4) where an agreement of 95% (t-test) with certified value was achieved. The precision of the method is better than 5% (relative standard deviation) for ten consecutive measurements of Hg. The method detection and quantification limits were 0.04 ug g-1 and 0.06 ug g-1, respectively, which meets the maximum concentration of Hg in fish established by ANVISA.O mercúrio (Hg) é um elemento tóxico que pode ficar retido no meio ambiente e na cadeia alimentar, resultado da sua biomagnificação e bioacumulação. Uma elevada concentração de Hg no organismo humano pode levar à sério risco para a saúde, comprometendo o sistema nervoso e o funcionamento de alguns órgãos. Uma das formas de contaminação por Hg é a partir da ingestão de alimentos contaminados. Diversos estudos relatam a contaminação do rio Madeira, localizado em Porto Velho – Rondônia, por Hg, o qual é utilizado na mineração do ouro em garimpos. Cabe destacar que há uma considerável população de ribeirinhos que utiliza o rio para a pesca. Portanto, o consumo de peixe contaminado pode ser uma questão de saúde pública, justificando o desenvolvimento de métodos analíticos para monitorar os níveis de concentração do Hg em peixe. Neste sentido, foi desenvolvido um sistema de introdução de amostra com geração química de vapor acoplado a espectrometria de massa com plasma indutivamente acoplado, para a determinação de Hg em músculo de peixes. No trabalho, foram avaliadas as condições reacionais e físicas do sistema proposto e analisadas 18 espécies de peixe com hábitos alimentares diferentes, tais como piscívoras, necrófagas, carnívoras, onívoras e herbívoras. Os resultados da concentração de Hg foram analisados de acordo com a legislação vigente no Brasil (ANVISA), onde são estipulados valores máximos de Hg de 1,0 g g-1 para espécies predadoras e 0,5 g g-1 para espécies não predadoras. A exatidão do método proposto foi avaliada utilizado material de referência certificado Dogfish Liver Certified Reference Material for Trace Metals (DOLT-4), obtendo-se uma concordância de 95% (teste t) com valor certificado. A precisão do método é melhor do que 5% (desvio padrão relativo) para dez medições consecutivas de Hg. Limites de detecção de 0,04 ug g-1 e quantificação de 0,06 ug g-1 foram obtidos pelo método proposto, o que atende a concentração máxima de Hg em peixe estabelecida pela ANVISA.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMercúrioPeixeRio MadeiraCVGICP-MSMercuryFishMadeira RiverCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICADesenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixeDevelopment of A FI-CVG-ICP-MS system for mercury determination in fishinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisDressler, Valderi Luizhttp://lattes.cnpq.br/4054740296547580Müller, Aline Lima HermesMortari, Sergio Robertohttp://lattes.cnpq.br/4214752797940876Viana, Ana Barbosa10060000000060060060060060029c59cfe-a489-425c-88a5-18fdf7cbf258a4023256-b7af-43a1-8b4e-5395967e64e13a5b86e4-a4fc-4124-b40b-b860a9eed50b3c621a6d-38be-4e61-b644-40b0cc92afcbreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/30281/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53ORIGINALDIS_PPGQUIMICA_2023_VIANA_ANA.pdfDIS_PPGQUIMICA_2023_VIANA_ANA.pdfDissertaçãoapplication/pdf1743488http://repositorio.ufsm.br/bitstream/1/30281/1/DIS_PPGQUIMICA_2023_VIANA_ANA.pdfc88d870c0665e93e63bb26ba998ba3d6MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/30281/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD521/302812023-09-28 10:12:04.286oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132023-09-28T13:12:04Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe |
dc.title.alternative.eng.fl_str_mv |
Development of A FI-CVG-ICP-MS system for mercury determination in fish |
title |
Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe |
spellingShingle |
Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe Viana, Ana Barbosa Mercúrio Peixe Rio Madeira CVG ICP-MS Mercury Fish Madeira River CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe |
title_full |
Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe |
title_fullStr |
Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe |
title_full_unstemmed |
Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe |
title_sort |
Desenvolvimento de sistema FI-CVG-ICP-MS para determinação de mercúrio em peixe |
author |
Viana, Ana Barbosa |
author_facet |
Viana, Ana Barbosa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Dressler, Valderi Luiz |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4054740296547580 |
dc.contributor.referee1.fl_str_mv |
Müller, Aline Lima Hermes |
dc.contributor.referee2.fl_str_mv |
Mortari, Sergio Roberto |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4214752797940876 |
dc.contributor.author.fl_str_mv |
Viana, Ana Barbosa |
contributor_str_mv |
Dressler, Valderi Luiz Müller, Aline Lima Hermes Mortari, Sergio Roberto |
dc.subject.por.fl_str_mv |
Mercúrio Peixe Rio Madeira CVG ICP-MS |
topic |
Mercúrio Peixe Rio Madeira CVG ICP-MS Mercury Fish Madeira River CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Mercury Fish Madeira River |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
Mercury (Hg) is a toxic element that can accumulate in the environment and in the food chain due to its biomagnification and bioaccumulation. A high concentration of Hg in the human body can lead to health complications, compromising mainly the nervous system and the functioning of some organs. One of the forms of contamination by Hg is from the ingestion of contaminated food. Several studies report the contamination of the Madeira River, located in Porto Velho - Rondônia, by Hg that is used in gold mining. It should be noted that there is a considerable population of riverine people who use the river for fishing. The consumption of contaminated fish can be a public health issue, justifying the development of analytical methods to monitor Hg concentration levels in fish. In this sense, a sample introduction system with chemical vapor generation coupled to inductively coupled plasma mass spectrometry was developed for Hg determination in fish muscle. The reaction conditions and physical variables of the proposed system were evaluated and eighteen species of fish with different eating habits were analyzed, such as piscivores, scavengers, carnivores, omnivores and herbivores. The results were analyzed in accordance with current legislation in Brazil (ANVISA), which specifies maximum Hg values of 1.0 g g-1 for predatory species and 0.5 g g-1 for non-predatory species. The accuracy of the proposed method was evaluated using certified reference material Dogfish Liver Certified Reference Material for Trace Metals (DOLT-4) where an agreement of 95% (t-test) with certified value was achieved. The precision of the method is better than 5% (relative standard deviation) for ten consecutive measurements of Hg. The method detection and quantification limits were 0.04 ug g-1 and 0.06 ug g-1, respectively, which meets the maximum concentration of Hg in fish established by ANVISA. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-09-28T13:12:04Z |
dc.date.available.fl_str_mv |
2023-09-28T13:12:04Z |
dc.date.issued.fl_str_mv |
2023-06-26 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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http://repositorio.ufsm.br/handle/1/30281 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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Programa de Pós-Graduação em Química |
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UFSM |
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Brasil |
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Química |
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Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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