Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5968 |
Resumo: | This work aimed to develop nanoemulsions and polymeric nanocapsules containing tioconazole for the topical treatment of superficial mycoses. Initially, a method was validated by liquid chromatography with UV detection to quantify the tioconazole in the nanostructured formulations. The method proved to be specific, linear, precise, accurate and robust. The nanoemulsions and tioconazole-loaded PCL nanocapsules (1.0 mg/mL) were prepared by spontaneous emulsification and interfacial deposition of preformed polymer methods, respectively, and using as oily core medium chain triglycerides or green coffee oil. The physicochemical parameters evaluated were: particle size, polydispersity index, pH, zeta potential, drug content and encapsulation efficiency. Formulations presented nanometric mean size (150-200 nm), polydispersity index below 0.15, acid pH (5.5 to 6.3), negative zeta potential (-3.5 to -11.3 mV), drug content close to the theoretical and encapsulation efficiency close to 100%. With the exception of pH, all these parameters remained the same after 30 days of storage, proving the stability of systems. The study of photodegradation of tioconazole against UVC light has shown the importance of nanostructured photoprotection in the drug and the greater protection was observed for the nanocapsules containing green coffee oil. The antifungal activity of the nanostructures was evaluated by agar diffusion and the results showed that the formulations presented antifungal activity against the yeast C. albicans, C. glabrata and clinical isolate of C. albicans. The nanostructures were incorporated into Aristoflex® AVC hydrogels and those were characterized by size of particles when dispersed in water, polydispersity index, drug content, spreadability, rheological properties and stability. The hydrogels had nanometer size (196-203 nm), polydispersity index below 0.25, pH 6,3 to 6.5, tioconazole content close to theoretical (0.99 to 1.01 mg/g) plastic behavior (model Casson) and similar spreadability profiles. The formulations were stable for 30 days at room temperature, evidencing the importance of the polymeric wall in the nanocapsules to preserve the characteristics of the formulations. The study of the in vitro release of drug from the hydrogel was carried out using diffusion Franz cell. The results demonstrated the ability of nanostructures to control the release of tioconazole, with greater control observed for hydrogels containing polymeric nanocapsules. |
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2015-02-092015-02-092013-06-21HÄRTER, Andréia Pisching Garcia. DEVELOPMENT OF NANOEMULSIONS AND POLYMERIC NANOCAPSULES CONTAINING TIOCONAZOLE AND INCORPORATION IN HYDROGELS. 2013. 127 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2013.http://repositorio.ufsm.br/handle/1/5968This work aimed to develop nanoemulsions and polymeric nanocapsules containing tioconazole for the topical treatment of superficial mycoses. Initially, a method was validated by liquid chromatography with UV detection to quantify the tioconazole in the nanostructured formulations. The method proved to be specific, linear, precise, accurate and robust. The nanoemulsions and tioconazole-loaded PCL nanocapsules (1.0 mg/mL) were prepared by spontaneous emulsification and interfacial deposition of preformed polymer methods, respectively, and using as oily core medium chain triglycerides or green coffee oil. The physicochemical parameters evaluated were: particle size, polydispersity index, pH, zeta potential, drug content and encapsulation efficiency. Formulations presented nanometric mean size (150-200 nm), polydispersity index below 0.15, acid pH (5.5 to 6.3), negative zeta potential (-3.5 to -11.3 mV), drug content close to the theoretical and encapsulation efficiency close to 100%. With the exception of pH, all these parameters remained the same after 30 days of storage, proving the stability of systems. The study of photodegradation of tioconazole against UVC light has shown the importance of nanostructured photoprotection in the drug and the greater protection was observed for the nanocapsules containing green coffee oil. The antifungal activity of the nanostructures was evaluated by agar diffusion and the results showed that the formulations presented antifungal activity against the yeast C. albicans, C. glabrata and clinical isolate of C. albicans. The nanostructures were incorporated into Aristoflex® AVC hydrogels and those were characterized by size of particles when dispersed in water, polydispersity index, drug content, spreadability, rheological properties and stability. The hydrogels had nanometer size (196-203 nm), polydispersity index below 0.25, pH 6,3 to 6.5, tioconazole content close to theoretical (0.99 to 1.01 mg/g) plastic behavior (model Casson) and similar spreadability profiles. The formulations were stable for 30 days at room temperature, evidencing the importance of the polymeric wall in the nanocapsules to preserve the characteristics of the formulations. The study of the in vitro release of drug from the hydrogel was carried out using diffusion Franz cell. The results demonstrated the ability of nanostructures to control the release of tioconazole, with greater control observed for hydrogels containing polymeric nanocapsules.Este trabalho objetivou o desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol para o tratamento tópico de micoses superficiais. Inicialmente, foi validado um método por cromatografia líquida com detecção UV para quantificação do tioconazol nas formulações nanoestruturadas. O método apresentou-se específico, linear, preciso, exato e robusto. As nanoemulsões e nanocápsulas de PCL contendo o tioconazol (1,0 mg/mL) foram preparadas pelos métodos de emulsificação espontânea e deposição interfacial do polímero pré-formado, respectivamente, utilizando como núcleo oleoso os triglicerídeos de cadeia média ou o óleo de café verde. Os parâmetros físico-químicos avaliados foram tamanho de partículas, índice de polidispersão, pH, potencial zeta, teor e eficiência de encapsulamento. As formulações apresentaram tamanho nanométrico (150 - 200 nm), índice de polidispersão abaixo de 0,15, pH ácido (5,5 a 6,3), potencial zeta negativo (-3,5 a -11,3 mV), teor de fármaco próximo ao teórico e eficiência de encapsulamento próximo a 100%. Com exceção do pH, todos esses parâmetros mantiveram-se iguais após os 30 dias de armazenamento a temperatura ambiente, comprovando a estabilidade dos sistemas. O estudo de fotodegradação do tioconazol frente à luz UVC demonstrou a importância dos sistemas nanoestruturados na fotoproteção do fármaco e uma maior proteção foi verificada para as nanocápsulas contendo óleo de café verde. A atividade antifúngica das nanoestruturas foi avaliada pelo método de difusão em ágar e os resultados demonstraram que as formulações apresentaram ação frente à C. albicans, C. glabrata e isolado clínico de C. albicans. As nanoestruturas foram incorporadas em hidrogéis de Aristoflex® AVC e estes caracterizados quanto ao tamanho de partículas após dispersão em água, índice de polidispersão, teor, espalhabilidade, propriedades reológicas e estabilidade frente ao armazenamento. Os hidrogéis apresentaram tamanho nanométrico (196 - 203 nm), índice de polidispersão abaixo de 0,25, pH de 6,3 a 6,5, teor de tioconazol próximo ao teórico (0,99 a 1,01 mg/g), comportamento plástico (modelo de Casson) e perfis de espalhabilidade semelhantes. As formulações mantiveram-se estáveis por 30 dias à temperatura ambiente, sendo evidenciada a importância da parede polimérica nas nanocápsulas em preservar as características das formulações. O estudo de liberação in vitro do fármaco a partir dos hidrogéis foi realizado empregando célula de difusão do tipo Franz. Os resultados obtidos demonstraram a capacidade das nanoestruturas em controlar a liberação do tioconazol, sendo que um maior controle foi observado para os hidrogéis contendo as nanocápsulas poliméricas.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBRFarmáciaHidrogéisNanocápsulasNanoemulsõesTioconazolHydrogelsNanocapsulesNanoemulsionsTioconazoleCNPQ::CIENCIAS DA SAUDE::FARMACIADesenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéisDevelopment of nanoemulsions and polymeric nanocapsules containing tioconazole and incorporation in hydrogelsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSilva, Cristiane de Bona dahttp://lattes.cnpq.br/6029111646602279Angeli, Valéria Weisshttp://lattes.cnpq.br/8514911514485464Rolim, Clarice Madalena Buenohttp://lattes.cnpq.br/2270654658839508http://lattes.cnpq.br/3507748842622293Härter, Andréia Pisching Garcia20100000000040030030030050077eeaef8-05f4-4cee-b949-c09342e7eb6167458976-d834-460b-a94a-787bc108db929e819cfd-a84f-43fb-b280-b03cf5a2b4b779341831-9e72-4135-835f-74dc53162e0einfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALHARTER, ANDREIA PISCHING GARCIA.pdfapplication/pdf1562718http://repositorio.ufsm.br/bitstream/1/5968/1/HARTER%2c%20ANDREIA%20PISCHING%20GARCIA.pdff0fbe01976ce5b66f7e68b08deece1b6MD51TEXTHARTER, ANDREIA PISCHING GARCIA.pdf.txtHARTER, ANDREIA PISCHING GARCIA.pdf.txtExtracted texttext/plain247697http://repositorio.ufsm.br/bitstream/1/5968/2/HARTER%2c%20ANDREIA%20PISCHING%20GARCIA.pdf.txt29aab2f02c5823601b098903679828a1MD52THUMBNAILHARTER, ANDREIA PISCHING GARCIA.pdf.jpgHARTER, ANDREIA PISCHING GARCIA.pdf.jpgIM Thumbnailimage/jpeg5387http://repositorio.ufsm.br/bitstream/1/5968/3/HARTER%2c%20ANDREIA%20PISCHING%20GARCIA.pdf.jpg25bcc85c73e639b83d09129e13f391b4MD531/59682022-10-14 15:51:07.815oai:repositorio.ufsm.br:1/5968Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-10-14T18:51:07Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis |
dc.title.alternative.eng.fl_str_mv |
Development of nanoemulsions and polymeric nanocapsules containing tioconazole and incorporation in hydrogels |
title |
Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis |
spellingShingle |
Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis Härter, Andréia Pisching Garcia Hidrogéis Nanocápsulas Nanoemulsões Tioconazol Hydrogels Nanocapsules Nanoemulsions Tioconazole CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis |
title_full |
Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis |
title_fullStr |
Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis |
title_full_unstemmed |
Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis |
title_sort |
Desenvolvimento de nanoemulsões e nanocápsulas poliméricas contendo tioconazol e incorporação em hidrogéis |
author |
Härter, Andréia Pisching Garcia |
author_facet |
Härter, Andréia Pisching Garcia |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Cristiane de Bona da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6029111646602279 |
dc.contributor.referee1.fl_str_mv |
Angeli, Valéria Weiss |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8514911514485464 |
dc.contributor.referee2.fl_str_mv |
Rolim, Clarice Madalena Bueno |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2270654658839508 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3507748842622293 |
dc.contributor.author.fl_str_mv |
Härter, Andréia Pisching Garcia |
contributor_str_mv |
Silva, Cristiane de Bona da Angeli, Valéria Weiss Rolim, Clarice Madalena Bueno |
dc.subject.por.fl_str_mv |
Hidrogéis Nanocápsulas Nanoemulsões Tioconazol |
topic |
Hidrogéis Nanocápsulas Nanoemulsões Tioconazol Hydrogels Nanocapsules Nanoemulsions Tioconazole CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Hydrogels Nanocapsules Nanoemulsions Tioconazole |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
This work aimed to develop nanoemulsions and polymeric nanocapsules containing tioconazole for the topical treatment of superficial mycoses. Initially, a method was validated by liquid chromatography with UV detection to quantify the tioconazole in the nanostructured formulations. The method proved to be specific, linear, precise, accurate and robust. The nanoemulsions and tioconazole-loaded PCL nanocapsules (1.0 mg/mL) were prepared by spontaneous emulsification and interfacial deposition of preformed polymer methods, respectively, and using as oily core medium chain triglycerides or green coffee oil. The physicochemical parameters evaluated were: particle size, polydispersity index, pH, zeta potential, drug content and encapsulation efficiency. Formulations presented nanometric mean size (150-200 nm), polydispersity index below 0.15, acid pH (5.5 to 6.3), negative zeta potential (-3.5 to -11.3 mV), drug content close to the theoretical and encapsulation efficiency close to 100%. With the exception of pH, all these parameters remained the same after 30 days of storage, proving the stability of systems. The study of photodegradation of tioconazole against UVC light has shown the importance of nanostructured photoprotection in the drug and the greater protection was observed for the nanocapsules containing green coffee oil. The antifungal activity of the nanostructures was evaluated by agar diffusion and the results showed that the formulations presented antifungal activity against the yeast C. albicans, C. glabrata and clinical isolate of C. albicans. The nanostructures were incorporated into Aristoflex® AVC hydrogels and those were characterized by size of particles when dispersed in water, polydispersity index, drug content, spreadability, rheological properties and stability. The hydrogels had nanometer size (196-203 nm), polydispersity index below 0.25, pH 6,3 to 6.5, tioconazole content close to theoretical (0.99 to 1.01 mg/g) plastic behavior (model Casson) and similar spreadability profiles. The formulations were stable for 30 days at room temperature, evidencing the importance of the polymeric wall in the nanocapsules to preserve the characteristics of the formulations. The study of the in vitro release of drug from the hydrogel was carried out using diffusion Franz cell. The results demonstrated the ability of nanostructures to control the release of tioconazole, with greater control observed for hydrogels containing polymeric nanocapsules. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-06-21 |
dc.date.accessioned.fl_str_mv |
2015-02-09 |
dc.date.available.fl_str_mv |
2015-02-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
HÄRTER, Andréia Pisching Garcia. DEVELOPMENT OF NANOEMULSIONS AND POLYMERIC NANOCAPSULES CONTAINING TIOCONAZOLE AND INCORPORATION IN HYDROGELS. 2013. 127 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5968 |
identifier_str_mv |
HÄRTER, Andréia Pisching Garcia. DEVELOPMENT OF NANOEMULSIONS AND POLYMERIC NANOCAPSULES CONTAINING TIOCONAZOLE AND INCORPORATION IN HYDROGELS. 2013. 127 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
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http://repositorio.ufsm.br/handle/1/5968 |
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por |
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201000000000 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Santa Maria |
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UFSM |
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BR |
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Farmácia |
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Universidade Federal de Santa Maria |
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