Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11114 |
Resumo: | Pain is the most common complaint in the medical field and the identification of compounds that can effectively treat painful states without induction of side-effects remains a major challenge in biomedical research. The aim of the present study was to investigate the antinociceptive effect of a novel compound, 3-(4-fluorophenyl)-5-trifluoromethyl-1H-1-tosylpyrazole (compound A) in several models of pain in mice and compare with those produced by the known trifluoromethyl-containing pyrazole compound celecoxib. Compound A or celecoxib were administrated by oral (78 780 μmol/kg), intrathecal (9 22.5 nmol/site) or intracerebroventricular (9 22.5 nmol/site) routes. Oral administration of either compound A or celecoxib abolished the mechanical allodynia, but not the oedema caused by intraplantar injection of carrageenan. Similarly, compound A reduced the overt nociception, but not the oedema, produced by bradykinin or capsaicin. However, compound A (500 μmol/kg, orally) did not alter nociception nor oedema caused by intraplantar injection of prostaglandin E2 or glutamate, whereas celecoxib reduced only the nociception induced by the former. Moreover, oral and intrathecal administration of compound A or celecoxib also reduced the nociception induced by acetic acid. However, only celecoxib reduced the acetic acid-induced nociception when it was injected by the intracerebroventricular route. Finally, neither compound A nor celecoxib was able to produce antinociceptive effect in the tail-flick test or to alter the motor performance and the body temperature. Besides, compound A or celecoxib did not induce gastric lesion. Thus, compound A seems to be an interesting prototype for the development of novel analgesic drugs. |
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2017-05-022017-05-022009-12-18OLIVEIRA, Sara Marchesan de. Study of the analgesic and antiinflammatory potential of 3-(4-fluorophenyl)-5-trifluoromethyl-1h-1-tosylpyrazole in pain models in mice. 2009. 60 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009.http://repositorio.ufsm.br/handle/1/11114Pain is the most common complaint in the medical field and the identification of compounds that can effectively treat painful states without induction of side-effects remains a major challenge in biomedical research. The aim of the present study was to investigate the antinociceptive effect of a novel compound, 3-(4-fluorophenyl)-5-trifluoromethyl-1H-1-tosylpyrazole (compound A) in several models of pain in mice and compare with those produced by the known trifluoromethyl-containing pyrazole compound celecoxib. Compound A or celecoxib were administrated by oral (78 780 μmol/kg), intrathecal (9 22.5 nmol/site) or intracerebroventricular (9 22.5 nmol/site) routes. Oral administration of either compound A or celecoxib abolished the mechanical allodynia, but not the oedema caused by intraplantar injection of carrageenan. Similarly, compound A reduced the overt nociception, but not the oedema, produced by bradykinin or capsaicin. However, compound A (500 μmol/kg, orally) did not alter nociception nor oedema caused by intraplantar injection of prostaglandin E2 or glutamate, whereas celecoxib reduced only the nociception induced by the former. Moreover, oral and intrathecal administration of compound A or celecoxib also reduced the nociception induced by acetic acid. However, only celecoxib reduced the acetic acid-induced nociception when it was injected by the intracerebroventricular route. Finally, neither compound A nor celecoxib was able to produce antinociceptive effect in the tail-flick test or to alter the motor performance and the body temperature. Besides, compound A or celecoxib did not induce gastric lesion. Thus, compound A seems to be an interesting prototype for the development of novel analgesic drugs.A dor é uma das maiores queixas na área médica e a identificação de compostos que possam tratar efetivamente os estados dolorosos, sem induzir efeitos colaterais, permanece um grande desafio na pesquisa biomédica. O objetivo do presente estudo foi investigar o efeito antinociceptivo de um novo composto 3-(4-fluorfenil)-5-trifluormetil-1H-1-tosilpirazol (composto A) em diferentes modelos de dor em camundongos e comparar os seus efeitos com aqueles produzidos por um pirazol contendo trifluormetil em sua estrutura, o celecoxibe. O composto A ou o celecoxibe foram administrados por via oral (78 780 μmol/kg), intratecal (9 22,5 nmol/sitio) ou intracerebroventricular (9 22,5 nmol/sitio). A administração oral do composto A ou do celecoxibe aboliram a alodínia mecânica, mas não o edema, causado pela injeção intraplantar de carragenina. Do mesmo modo, a administração do composto A reduziu a nocicepção, mas não o edema, produzido pela bradicinina ou capsaicina. Entretanto, o composto A administrado por via oral (500 μmol/kg) não alterou a nocicepção nem o edema causados pela injeção intraplantar de prostaglandina E2 (PGE2) ou glutamato. Já o celecoxibe, reduziu somente a nocicepção induzida pela PGE2. Além disso, a administração oral ou intratecal do composto A ou celecoxibe também reduziu a nocicepção induzida por ácido acético. Quando administrados por via intracerebroventricular, somente o celecoxibe foi capaz de reduzir a nocicepção induzida por ácido acético. Finalmente, o composto A ou o celecoxibe não alteraram a nocicepção térmica aguda, a performance motora ou a temperatura corporal dos animais, nem produziram lesões gástricas quando administrados por via oral. Consequentemente, o composto A parece ser um interessante protótipo para o desenvolvimento de novas drogas analgésicas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaDorNocicepçãoInflamaçãoEdemaPirazóisAnalgésicosPainNociceptionInflammationEdemaPyrazolesAnalgesicsCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEstudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongosStudy of the analgesic and antiinflammatory potential of 3-(4-fluorophenyl)-5-trifluoromethyl-1h-1-tosylpyrazole in pain models in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisFerreira, Julianohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768702Y6Otuki, Michel Fleithhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765550U6Oliveira, Mauro Schneiderhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705848A9http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4268855J0Oliveira, Sara Marchesan de20080000000240050050050050000296dee-06fe-4bc6-a73d-5a0cdd89e3bc30006666-6c66-477b-8960-e63c8b48bf63030b4439-260c-4e8b-bdd3-86c310cdcfabcf98ddcb-3930-42cf-9437-be60eebd8056info:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALOLIVEIRA, SARA MARCHESAN DE.pdfapplication/pdf2513069http://repositorio.ufsm.br/bitstream/1/11114/1/OLIVEIRA%2c%20SARA%20MARCHESAN%20DE.pdfafcfcfb515f7aed15b8f6c8fe6dc08d8MD51TEXTOLIVEIRA, SARA MARCHESAN DE.pdf.txtOLIVEIRA, SARA MARCHESAN DE.pdf.txtExtracted texttext/plain73678http://repositorio.ufsm.br/bitstream/1/11114/2/OLIVEIRA%2c%20SARA%20MARCHESAN%20DE.pdf.txt9b849c25493b1ce02c8deb4ae6fe1e73MD52THUMBNAILOLIVEIRA, SARA MARCHESAN DE.pdf.jpgOLIVEIRA, SARA MARCHESAN DE.pdf.jpgIM Thumbnailimage/jpeg6219http://repositorio.ufsm.br/bitstream/1/11114/3/OLIVEIRA%2c%20SARA%20MARCHESAN%20DE.pdf.jpg8cc0c30f59dd5c94de8e891f28d91286MD531/111142017-07-25 12:09:34.645oai:repositorio.ufsm.br:1/11114Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132017-07-25T15:09:34Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos |
dc.title.alternative.eng.fl_str_mv |
Study of the analgesic and antiinflammatory potential of 3-(4-fluorophenyl)-5-trifluoromethyl-1h-1-tosylpyrazole in pain models in mice |
title |
Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos |
spellingShingle |
Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos Oliveira, Sara Marchesan de Dor Nocicepção Inflamação Edema Pirazóis Analgésicos Pain Nociception Inflammation Edema Pyrazoles Analgesics CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos |
title_full |
Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos |
title_fullStr |
Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos |
title_full_unstemmed |
Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos |
title_sort |
Estudo do potencial analgésico e antiinflamatório do 3-(4-fluorfenil)-5-trifluormetil-1h-1-tosilpirazol em modelos de dor em camundongos |
author |
Oliveira, Sara Marchesan de |
author_facet |
Oliveira, Sara Marchesan de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferreira, Juliano |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768702Y6 |
dc.contributor.referee1.fl_str_mv |
Otuki, Michel Fleith |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765550U6 |
dc.contributor.referee2.fl_str_mv |
Oliveira, Mauro Schneider |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705848A9 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4268855J0 |
dc.contributor.author.fl_str_mv |
Oliveira, Sara Marchesan de |
contributor_str_mv |
Ferreira, Juliano Otuki, Michel Fleith Oliveira, Mauro Schneider |
dc.subject.por.fl_str_mv |
Dor Nocicepção Inflamação Edema Pirazóis Analgésicos |
topic |
Dor Nocicepção Inflamação Edema Pirazóis Analgésicos Pain Nociception Inflammation Edema Pyrazoles Analgesics CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Pain Nociception Inflammation Edema Pyrazoles Analgesics |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Pain is the most common complaint in the medical field and the identification of compounds that can effectively treat painful states without induction of side-effects remains a major challenge in biomedical research. The aim of the present study was to investigate the antinociceptive effect of a novel compound, 3-(4-fluorophenyl)-5-trifluoromethyl-1H-1-tosylpyrazole (compound A) in several models of pain in mice and compare with those produced by the known trifluoromethyl-containing pyrazole compound celecoxib. Compound A or celecoxib were administrated by oral (78 780 μmol/kg), intrathecal (9 22.5 nmol/site) or intracerebroventricular (9 22.5 nmol/site) routes. Oral administration of either compound A or celecoxib abolished the mechanical allodynia, but not the oedema caused by intraplantar injection of carrageenan. Similarly, compound A reduced the overt nociception, but not the oedema, produced by bradykinin or capsaicin. However, compound A (500 μmol/kg, orally) did not alter nociception nor oedema caused by intraplantar injection of prostaglandin E2 or glutamate, whereas celecoxib reduced only the nociception induced by the former. Moreover, oral and intrathecal administration of compound A or celecoxib also reduced the nociception induced by acetic acid. However, only celecoxib reduced the acetic acid-induced nociception when it was injected by the intracerebroventricular route. Finally, neither compound A nor celecoxib was able to produce antinociceptive effect in the tail-flick test or to alter the motor performance and the body temperature. Besides, compound A or celecoxib did not induce gastric lesion. Thus, compound A seems to be an interesting prototype for the development of novel analgesic drugs. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-12-18 |
dc.date.accessioned.fl_str_mv |
2017-05-02 |
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2017-05-02 |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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dc.identifier.citation.fl_str_mv |
OLIVEIRA, Sara Marchesan de. Study of the analgesic and antiinflammatory potential of 3-(4-fluorophenyl)-5-trifluoromethyl-1h-1-tosylpyrazole in pain models in mice. 2009. 60 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11114 |
identifier_str_mv |
OLIVEIRA, Sara Marchesan de. Study of the analgesic and antiinflammatory potential of 3-(4-fluorophenyl)-5-trifluoromethyl-1h-1-tosylpyrazole in pain models in mice. 2009. 60 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009. |
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