Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/18973 |
Resumo: | Hyperlipidemia (HL) is characterized by changes in lipid metabolism, being one of the main metabolic disorders. HL is recognized as a risk factor for the development of atherosclerosis, characterized by the presence of endothelial dysfunction and an inflammatory process. Extracellular nucleotides and nucleosides are important signaling molecules that modulate the actions of lymphocytes and are essential for the initiation and maintenance of inflammatory reactions. Extracellular levels of these nucleotides are physiologically controlled by the action of an enzyme complex formed by enzymes E-NTPDase, E-5'nucleotidase and E-ADA. These enzymes are located on the surface of lymphocytes and play an important role in maintaining homeostasis. The inflammatory process and the immune response are involved in the formation of atherosclerotic lesions. Cytokines are responsible for modulating aspects of vascular inflammation by altering the proliferation, differentiation and vascular function of a variety of cell types in which intercellular communication occurs. Moreover, changes in cholesterol homeostasis also act negatively on the central nervous system. Acetylcholine is one of the main neurotransmitters of the cholinergic system and is hydrolyzed by acetylcholinesterase, an important regulatory enzyme. AChE is very important in controlling the transmission of nerve impulses through synapses and is therefore considered a good indicator of cholinergic activity. Changes in AChE activity have been associated with memory and learning deficits. In view of this, the interest in disease prevention through food is increasing. Quercetin, present in apples, onions, broccoli, among others, has been studied for presenting antioxidant, anti-inflammatory and neuroprotective actions. Thus, the objective of this study was to evaluate whether the preventive treatment of quercetin is able to prevent the changes caused by HL in the purinergic and cholinergic systems. Male Wistar rats were used and divided into ten groups (n=7), five of them without induction of HL and five with induction. Groups were pretreated with quercetin at doses of 5, 25 and 50 mg / kg for 30 days. After 30 days, P407 (500 mg / kg) was administrated intraperitoneally for induction of HL. Simvastatin (0.04 mg / kg) was also administered after induction as a positive control. The results showed that the preventive treatment with quercetin could reduce the elevated lipid levels induced by HL (P˂0.05). It was also observed in the object recognition test, that there was a significant decrease in the memory of hyperlipidemic rats (P˂0.001), and this alteration was smoothed with the prevention of the preventive treatment of quercetin (P˂0.05). The HL also decreased acetylcholinesterase activity in 52.6% in the hippocampus of hyperlipidemic rats. An increase in E-NTPDase activity of 72% in the ATP hydrolysis) and 98.7% in the ADP hydrolysis the 78% in the E-ADA activity in lymphocytes of hyperlipidemic rats was also demonstrated and pretreatment with quercetin avoided this increase (P˂0.01). Levels of IFN-γ and IL-4 were observed in hiperlipidemic rats (P˂0.01), and that these levels were decreased with quercetin pretreatment (P˂0.05), demonstrating its anti-inflammatory effect. Thus, we conclude that the preventive treatment of quercetin possibly inhibits the inflammatory pathways, reducing the levels of proinflammatory cytokines, modulating the purinergic system and also reversing the changes caused in the HL-induced cholinergic system. |
id |
UFSM-20_3ad890ce5fc1c796ec117d42d00df360 |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/18973 |
network_acronym_str |
UFSM-20 |
network_name_str |
Repositório Institucional Manancial UFSM |
repository_id_str |
3913 |
spelling |
2019-11-19T18:55:54Z2019-11-19T18:55:54Z2017-08-30http://repositorio.ufsm.br/handle/1/18973Hyperlipidemia (HL) is characterized by changes in lipid metabolism, being one of the main metabolic disorders. HL is recognized as a risk factor for the development of atherosclerosis, characterized by the presence of endothelial dysfunction and an inflammatory process. Extracellular nucleotides and nucleosides are important signaling molecules that modulate the actions of lymphocytes and are essential for the initiation and maintenance of inflammatory reactions. Extracellular levels of these nucleotides are physiologically controlled by the action of an enzyme complex formed by enzymes E-NTPDase, E-5'nucleotidase and E-ADA. These enzymes are located on the surface of lymphocytes and play an important role in maintaining homeostasis. The inflammatory process and the immune response are involved in the formation of atherosclerotic lesions. Cytokines are responsible for modulating aspects of vascular inflammation by altering the proliferation, differentiation and vascular function of a variety of cell types in which intercellular communication occurs. Moreover, changes in cholesterol homeostasis also act negatively on the central nervous system. Acetylcholine is one of the main neurotransmitters of the cholinergic system and is hydrolyzed by acetylcholinesterase, an important regulatory enzyme. AChE is very important in controlling the transmission of nerve impulses through synapses and is therefore considered a good indicator of cholinergic activity. Changes in AChE activity have been associated with memory and learning deficits. In view of this, the interest in disease prevention through food is increasing. Quercetin, present in apples, onions, broccoli, among others, has been studied for presenting antioxidant, anti-inflammatory and neuroprotective actions. Thus, the objective of this study was to evaluate whether the preventive treatment of quercetin is able to prevent the changes caused by HL in the purinergic and cholinergic systems. Male Wistar rats were used and divided into ten groups (n=7), five of them without induction of HL and five with induction. Groups were pretreated with quercetin at doses of 5, 25 and 50 mg / kg for 30 days. After 30 days, P407 (500 mg / kg) was administrated intraperitoneally for induction of HL. Simvastatin (0.04 mg / kg) was also administered after induction as a positive control. The results showed that the preventive treatment with quercetin could reduce the elevated lipid levels induced by HL (P˂0.05). It was also observed in the object recognition test, that there was a significant decrease in the memory of hyperlipidemic rats (P˂0.001), and this alteration was smoothed with the prevention of the preventive treatment of quercetin (P˂0.05). The HL also decreased acetylcholinesterase activity in 52.6% in the hippocampus of hyperlipidemic rats. An increase in E-NTPDase activity of 72% in the ATP hydrolysis) and 98.7% in the ADP hydrolysis the 78% in the E-ADA activity in lymphocytes of hyperlipidemic rats was also demonstrated and pretreatment with quercetin avoided this increase (P˂0.01). Levels of IFN-γ and IL-4 were observed in hiperlipidemic rats (P˂0.01), and that these levels were decreased with quercetin pretreatment (P˂0.05), demonstrating its anti-inflammatory effect. Thus, we conclude that the preventive treatment of quercetin possibly inhibits the inflammatory pathways, reducing the levels of proinflammatory cytokines, modulating the purinergic system and also reversing the changes caused in the HL-induced cholinergic system.A hiperlipidemia (HL) se caracteriza por alterações no metabolismo lipídico, sendo um dos principais distúrbios metabólicos. A HL é reconhecida como um dos fatores de risco para o desenvolvimento da aterosclerose, sendo esta caracterizada pela presença de disfunção endotelial e por um processo inflamatório. Os nucleotídeos e nucleosídeo extracelulares são importantes moléculas sinalizadoras que modulam as ações dos linfócitos, sendo essenciais para o início e manutenção das reações inflamatórias. Os níveis extracelulares destes nucleotídeos são fisiologicamente controlados pela ação de um complexo enzimático formado pelas enzimas E-NTPDase, E-5‘nucleotidase e E-ADA. Estas enzimas estão localizadas na superfície de linfócitos e desempenham um papel importante na manutenção da homeostasia. O processo inflamatório e a resposta imune estão envolvidos na formação da lesão aterosclerótica. As citocinas são responsáveis pela modulação de aspectos da inflamação vascular, alterando a proliferação, diferenciação e função vascular de uma variedade de tipos celulares em que ocorre a comunicação intercelular. Além disto, alterações na homeostase do colesterol também agem negativamente no sistema nervoso central. A acetilcolina é um dos principais neurotransmissores do sistema colinérgico, e é hidrolisada pela acetilcolinesterase, importante enzima regulatória. A AChE é muito importante no controle da transmissão dos impulsos nervosos através das sinapses, sendo por isso considerada um bom indicador da atividade colinérgica. Alterações na atividade da AChE tem sido associadas a déficits de memória e aprendizagem. Diante disto, o interesse pela prevenção de doenças através da alimentação vem aumentando. A quercetina, presente em maçãs, cebolas, brócolis, entre outras, vem sendo estudada por apresentar ações antioxidantes, anti-inflamatórias e neuroprotetoras. Sendo assim, o objetivo deste trabalho foi avaliar se o tratamento preventivo de quercetina é capaz de prevenir as alterações causadas pela HL nos sistemas purinérgico e colinérgico. Foram utilizados ratos Wistar machos, e divididos em 10 grupos (n=7), sendo 5 sem indução da HL e 5 com a indução. Os grupos foram tratados preventivamente com quercetina nas doses de 5, 25 e 50 mg/kg, durante 30 dias. Após os 30 dias, foi administrado o P407 (500 mg/kg) via intraperitoneal, para indução da HL. Sinvastatina (0,04 mg/kg) também foi administrada após a indução como controle positivo. Os resultados monstraram que o tratamento preventivo de quercetina conseguiu amenizar os níveis elevados dos lipídios induzidos pela HL (P˂0,05). Também foi observado no teste do reconhecimento de objeto, que houve uma diminuição significativa na memória de ratos hiperlipidêmicos (P˂0,001), e esta alteração foi amenizada com o tratamento preventivo de quercetina (P˂0,05). A HL também diminuiu a atividade da acetilcolinesterase em 52,6% no hipocampo de ratos hiperlipidêmicos. Também demonstrou-se um aumento na atividade da E-NTPDase, em 72% na hidrólise de ATP e 98,7% na hidrólise de ADP e 78% na atividade da E-ADA em linfócitos dos ratos hiperlipidêmicos e o pré-tratamento com quercetina fez com que este aumento fosse evitado (P˂0,01). Níveis elevados de IFN-γ e IL-4 foram observados em ratos com HL (P˂0,01), e estes níveis foram diminuídos com o pré-tratamento de quercetina (P<0.05), demonstrando seu efeito anti-inflamatório. Dessa forma, podemos concluir que o tratamento preventivo de quercetina possivelmente inibe as vias inflamatórias, reduzindo os níveis de citocinas pró-inflamatórias, modulando o sistema purinérgico e também revertendo as alterações causadas no sistema colinérgico induzido pela HL.porUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessQuercetinaHiperlipidemiaSinalização purinérgicaSinalização colinérgicaQuercetinHyperlipidemiaPurinergic signalingCholinergic signalingCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemiaPreventive effect of quercetin flavonoid in purinergic and colinergic systems in experimental model of hyperlipidemicinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisLeal, Daniela Bitencourt Rosahttp://lattes.cnpq.br/3639683273462361Garcia, Solange Cristinahttp://lattes.cnpq.br/6687355709603379Jaques, Jeandre Augusto dos Santoshttp://lattes.cnpq.br/9733439439501163Bauermann, Liliane de Freitashttp://lattes.cnpq.br/5849925846135968Cruz, Ivana Beatrice Mânica Dahttp://lattes.cnpq.br/3426369324110716http://lattes.cnpq.br/0217671314699365Braun, Josiane Bizzi Schlemmer200800000002600ebd6d0bc-4e14-43fd-8d4e-ad9ebe640af2db6d2b95-5dca-4844-a673-a306a5dddf2308641410-3b67-4735-9a1f-8563f344fcd6e8092a1b-7197-4baf-bbef-7ceef075d117fd911925-2f77-4c9b-90e0-fe5f4b3b868455d5df61-bdaf-4677-bad9-6430c03f8250reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTES_PPGBT_2017_BRAUN_JOSIANE.pdfTES_PPGBT_2017_BRAUN_JOSIANE.pdfTese de Doutoradoapplication/pdf4071697http://repositorio.ufsm.br/bitstream/1/18973/1/TES_PPGBT_2017_BRAUN_JOSIANE.pdf804ad2340a3e1782c956b0f9f5e44c53MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/18973/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/18973/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53TEXTTES_PPGBT_2017_BRAUN_JOSIANE.pdf.txtTES_PPGBT_2017_BRAUN_JOSIANE.pdf.txtExtracted texttext/plain199877http://repositorio.ufsm.br/bitstream/1/18973/4/TES_PPGBT_2017_BRAUN_JOSIANE.pdf.txt75f916aa426111cf00fe3531f86e5599MD54THUMBNAILTES_PPGBT_2017_BRAUN_JOSIANE.pdf.jpgTES_PPGBT_2017_BRAUN_JOSIANE.pdf.jpgIM Thumbnailimage/jpeg4433http://repositorio.ufsm.br/bitstream/1/18973/5/TES_PPGBT_2017_BRAUN_JOSIANE.pdf.jpgc0c86251a41f04102637bde7e6d858c9MD551/189732019-11-20 03:02:22.641oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132019-11-20T06:02:22Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia |
dc.title.alternative.eng.fl_str_mv |
Preventive effect of quercetin flavonoid in purinergic and colinergic systems in experimental model of hyperlipidemic |
title |
Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia |
spellingShingle |
Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia Braun, Josiane Bizzi Schlemmer Quercetina Hiperlipidemia Sinalização purinérgica Sinalização colinérgica Quercetin Hyperlipidemia Purinergic signaling Cholinergic signaling CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia |
title_full |
Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia |
title_fullStr |
Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia |
title_full_unstemmed |
Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia |
title_sort |
Efeito preventivo do flavonoide quercetina nos sistemas purinérgico e colinérgico de modelo experimental de hiperlipidemia |
author |
Braun, Josiane Bizzi Schlemmer |
author_facet |
Braun, Josiane Bizzi Schlemmer |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Leal, Daniela Bitencourt Rosa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3639683273462361 |
dc.contributor.referee1.fl_str_mv |
Garcia, Solange Cristina |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6687355709603379 |
dc.contributor.referee2.fl_str_mv |
Jaques, Jeandre Augusto dos Santos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9733439439501163 |
dc.contributor.referee3.fl_str_mv |
Bauermann, Liliane de Freitas |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/5849925846135968 |
dc.contributor.referee4.fl_str_mv |
Cruz, Ivana Beatrice Mânica Da |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/3426369324110716 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0217671314699365 |
dc.contributor.author.fl_str_mv |
Braun, Josiane Bizzi Schlemmer |
contributor_str_mv |
Leal, Daniela Bitencourt Rosa Garcia, Solange Cristina Jaques, Jeandre Augusto dos Santos Bauermann, Liliane de Freitas Cruz, Ivana Beatrice Mânica Da |
dc.subject.por.fl_str_mv |
Quercetina Hiperlipidemia Sinalização purinérgica Sinalização colinérgica |
topic |
Quercetina Hiperlipidemia Sinalização purinérgica Sinalização colinérgica Quercetin Hyperlipidemia Purinergic signaling Cholinergic signaling CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Quercetin Hyperlipidemia Purinergic signaling Cholinergic signaling |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Hyperlipidemia (HL) is characterized by changes in lipid metabolism, being one of the main metabolic disorders. HL is recognized as a risk factor for the development of atherosclerosis, characterized by the presence of endothelial dysfunction and an inflammatory process. Extracellular nucleotides and nucleosides are important signaling molecules that modulate the actions of lymphocytes and are essential for the initiation and maintenance of inflammatory reactions. Extracellular levels of these nucleotides are physiologically controlled by the action of an enzyme complex formed by enzymes E-NTPDase, E-5'nucleotidase and E-ADA. These enzymes are located on the surface of lymphocytes and play an important role in maintaining homeostasis. The inflammatory process and the immune response are involved in the formation of atherosclerotic lesions. Cytokines are responsible for modulating aspects of vascular inflammation by altering the proliferation, differentiation and vascular function of a variety of cell types in which intercellular communication occurs. Moreover, changes in cholesterol homeostasis also act negatively on the central nervous system. Acetylcholine is one of the main neurotransmitters of the cholinergic system and is hydrolyzed by acetylcholinesterase, an important regulatory enzyme. AChE is very important in controlling the transmission of nerve impulses through synapses and is therefore considered a good indicator of cholinergic activity. Changes in AChE activity have been associated with memory and learning deficits. In view of this, the interest in disease prevention through food is increasing. Quercetin, present in apples, onions, broccoli, among others, has been studied for presenting antioxidant, anti-inflammatory and neuroprotective actions. Thus, the objective of this study was to evaluate whether the preventive treatment of quercetin is able to prevent the changes caused by HL in the purinergic and cholinergic systems. Male Wistar rats were used and divided into ten groups (n=7), five of them without induction of HL and five with induction. Groups were pretreated with quercetin at doses of 5, 25 and 50 mg / kg for 30 days. After 30 days, P407 (500 mg / kg) was administrated intraperitoneally for induction of HL. Simvastatin (0.04 mg / kg) was also administered after induction as a positive control. The results showed that the preventive treatment with quercetin could reduce the elevated lipid levels induced by HL (P˂0.05). It was also observed in the object recognition test, that there was a significant decrease in the memory of hyperlipidemic rats (P˂0.001), and this alteration was smoothed with the prevention of the preventive treatment of quercetin (P˂0.05). The HL also decreased acetylcholinesterase activity in 52.6% in the hippocampus of hyperlipidemic rats. An increase in E-NTPDase activity of 72% in the ATP hydrolysis) and 98.7% in the ADP hydrolysis the 78% in the E-ADA activity in lymphocytes of hyperlipidemic rats was also demonstrated and pretreatment with quercetin avoided this increase (P˂0.01). Levels of IFN-γ and IL-4 were observed in hiperlipidemic rats (P˂0.01), and that these levels were decreased with quercetin pretreatment (P˂0.05), demonstrating its anti-inflammatory effect. Thus, we conclude that the preventive treatment of quercetin possibly inhibits the inflammatory pathways, reducing the levels of proinflammatory cytokines, modulating the purinergic system and also reversing the changes caused in the HL-induced cholinergic system. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-08-30 |
dc.date.accessioned.fl_str_mv |
2019-11-19T18:55:54Z |
dc.date.available.fl_str_mv |
2019-11-19T18:55:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18973 |
url |
http://repositorio.ufsm.br/handle/1/18973 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
ebd6d0bc-4e14-43fd-8d4e-ad9ebe640af2 db6d2b95-5dca-4844-a673-a306a5dddf23 08641410-3b67-4735-9a1f-8563f344fcd6 e8092a1b-7197-4baf-bbef-7ceef075d117 fd911925-2f77-4c9b-90e0-fe5f4b3b8684 55d5df61-bdaf-4677-bad9-6430c03f8250 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional Manancial UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Repositório Institucional Manancial UFSM |
collection |
Repositório Institucional Manancial UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/18973/1/TES_PPGBT_2017_BRAUN_JOSIANE.pdf http://repositorio.ufsm.br/bitstream/1/18973/2/license_rdf http://repositorio.ufsm.br/bitstream/1/18973/3/license.txt http://repositorio.ufsm.br/bitstream/1/18973/4/TES_PPGBT_2017_BRAUN_JOSIANE.pdf.txt http://repositorio.ufsm.br/bitstream/1/18973/5/TES_PPGBT_2017_BRAUN_JOSIANE.pdf.jpg |
bitstream.checksum.fl_str_mv |
804ad2340a3e1782c956b0f9f5e44c53 4460e5956bc1d1639be9ae6146a50347 2f0571ecee68693bd5cd3f17c1e075df 75f916aa426111cf00fe3531f86e5599 c0c86251a41f04102637bde7e6d858c9 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
ouvidoria@ufsm.br |
_version_ |
1808854714919419904 |